Restriction map for pF1KB8111
 (Linear) (Six-base) MAPSORT of: KIBB8111.seq  Check: 8814  from: 1  to: 1668



KIBB8111.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1149    1668 
   Size:       1149     519
 
AleI CACnn'nnGTG

Cuts at:      0    1599    1668 
   Size:       1599      69
 
ApaI G_GGCC'C

Cuts at:      0     145    1384    1668 
   Size:        145    1239     284

  Fragments arranged by size: 

                1239     284     145
 
ApoI r'AATT_y

Cuts at:      0     332     910    1490    1527    1668 
   Size:        332     578     580      37     141

  Fragments arranged by size: 

                 580     578     332     141      37
 
AvaI C'yCGr_G

Cuts at:      0     296    1668 
   Size:        296    1372
 
BamHI G'GATC_C

Cuts at:      0    1461    1668 
   Size:       1461     207
 
BanI G'GyrC_C

Cuts at:      0     181    1343    1668 
   Size:        181    1162     325

  Fragments arranged by size: 

                1162     325     181
 
BanII G_rGCy'C

Cuts at:      0     145     801    1384    1668 
   Size:        145     656     583     284

  Fragments arranged by size: 

                 656     583     284     145
 
BbeI G_GCGC'C

Cuts at:      0     185    1668 
   Size:        185    1483
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1106    1157    1668 
   Size:       1106      51     511

  Fragments arranged by size: 

                1106     511      51
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     988    1668 
   Size:        988     680
 
BfrBI ATG'CAT

Cuts at:      0     809    1668 
   Size:        809     859
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1148    1668 
   Size:       1148     520
 
Bme1580I G_kGCm'C

Cuts at:      0      38     145    1384    1668 
   Size:         38     107    1239     284

  Fragments arranged by size: 

                1239     284     107      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1378    1668 
   Size:       1378     290
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     358     734    1668 
   Size:        297      61     376     934

  Fragments arranged by size: 

                 934     376     297      61
 
Bpu10I CC'TnA_GC

Cuts at:      0    1138    1668 
   Size:       1138     530
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     529     923    1668 
   Size:        529     394     745

  Fragments arranged by size: 

                 745     529     394
 
BsaAI yAC'GTr

Cuts at:      0    1037    1668 
   Size:       1037     631
 
BsaHI Gr'CG_yC

Cuts at:      0      29     182    1060    1655    1668 
   Size:         29     153     878     595      13

  Fragments arranged by size: 

                 878     595     153      29      13
 
BsaWI w'CCGG_w

Cuts at:      0     621    1668 
   Size:        621    1047
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1249    1279    1668 
   Size:       1249      30     389

  Fragments arranged by size: 

                1249     389      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1164    1167    1668 
   Size:       1164       3     501

  Fragments arranged by size: 

                1164     501       3
 
BseYI C'CCAG_C

Cuts at:      0     203    1582    1668 
   Size:        203    1379      86

  Fragments arranged by size: 

                1379     203      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1033    1626    1668 
   Size:       1033     593      42

  Fragments arranged by size: 

                1033     593      42
 
BsmI GAATG_Cn'

Cuts at:      0     119    1668 
   Size:        119    1549
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     145     801    1384    1668 
   Size:         38     107     656     583     284

  Fragments arranged by size: 

                 656     583     284     107      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1055    1091    1149    1668 
   Size:       1055      36      58     519

  Fragments arranged by size: 

                1055     519      58      36
 
BsrBI CCG'CTC

Cuts at:      0     390    1668 
   Size:        390    1278
 
BsrFI r'CCGG_y

Cuts at:      0     184    1086    1668 
   Size:        184     902     582

  Fragments arranged by size: 

                 902     582     184
 
BssSI C'ACGA_G

Cuts at:      0     934    1668 
   Size:        934     734
 
BstYI r'GATC_y

Cuts at:      0    1461    1613    1668 
   Size:       1461     152      55

  Fragments arranged by size: 

                1461     152      55
 
Bsu36I CC'TnA_GG

Cuts at:      0     236     920    1668 
   Size:        236     684     748

  Fragments arranged by size: 

                 748     684     236
 
BtgI C'CryG_G

Cuts at:      0     149     281    1668 
   Size:        149     132    1387

  Fragments arranged by size: 

                1387     149     132
 
BtsI GCAGTG_nn'

Cuts at:      0     158    1668 
   Size:        158    1510
 
DraI TTT'AAA

Cuts at:      0    1533    1668 
   Size:       1533     135
 
EaeI y'GGCC_r

Cuts at:      0       2    1668 
   Size:          2    1666
 
EarI CTCTTCn'nnn_

Cuts at:      0    1297    1668 
   Size:       1297     371
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     200     762    1346    1668 
   Size:        200     562     584     322

  Fragments arranged by size: 

                 584     562     322     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     358     734    1668 
   Size:        297      61     376     934

  Fragments arranged by size: 

                 934     376     297      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     141     142     369     612    1668 
   Size:         89      52       1     227     243    1056

  Fragments arranged by size: 

                1056     243     227      89      52       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     453    1668 
   Size:        453    1215
 
HaeII r_GCGC'y

Cuts at:      0     185    1668 
   Size:        185    1483
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     385     417     553     585    1243    1249    1275    1281 
   Size:        385      32     136      32     658       6      26       6

Cuts at:   1281    1554    1586    1668 
   Size:        273      32      82

  Fragments arranged by size: 

                 658     385     273     136      82      32      32      32
                  26       6       6
 
HincII GTy'rAC

Cuts at:      0    1470    1668 
   Size:       1470     198
 
HindIII A'AGCT_T

Cuts at:      0     430    1668 
   Size:        430    1238
 
KasI G'GCGC_C

Cuts at:      0     181    1668 
   Size:        181    1487
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     869    1076    1223    1295    1668 
   Size:        869     207     147      72     373

  Fragments arranged by size: 

                 869     373     207     147      72
 
MscI TGG'CCA

Cuts at:      0       4    1668 
   Size:          4    1664
 
MslI CAynn'nnrTG

Cuts at:      0    1159    1599    1668 
   Size:       1159     440      69

  Fragments arranged by size: 

                1159     440      69
 
MspA1I CmG'CkG

Cuts at:      0      21    1425    1586    1660    1668 
   Size:         21    1404     161      74       8

  Fragments arranged by size: 

                1404     161      74      21       8
 
NaeI GCC'GGC

Cuts at:      0     186    1088    1668 
   Size:        186     902     580

  Fragments arranged by size: 

                 902     580     186
 
NarI GG'CG_CC

Cuts at:      0     182    1668 
   Size:        182    1486
 
NcoI C'CATG_G

Cuts at:      0     149    1668 
   Size:        149    1519
 
NgoMIV G'CCGG_C

Cuts at:      0     184    1086    1668 
   Size:        184     902     582

  Fragments arranged by size: 

                 902     582     184
 
NsiI A_TGCA'T

Cuts at:      0     811    1668 
   Size:        811     857
 
NspI r_CATG'y

Cuts at:      0     202    1158    1668 
   Size:        202     956     510

  Fragments arranged by size: 

                 956     510     202
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     825    1668 
   Size:        825     843
 
PfoI T'CCnGG_A

Cuts at:      0    1616    1668 
   Size:       1616      52
 
PmlI CAC'GTG

Cuts at:      0    1037    1668 
   Size:       1037     631
 
PpuMI rG'GwC_Cy

Cuts at:      0      89     369     612    1668 
   Size:         89     280     243    1056

  Fragments arranged by size: 

                1056     280     243      89
 
PspOMI G'GGCC_C

Cuts at:      0     141    1380    1668 
   Size:        141    1239     288

  Fragments arranged by size: 

                1239     288     141
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1265    1297    1668 
   Size:       1265      32     371

  Fragments arranged by size: 

                1265     371      32
 
PstI C_TGCA'G

Cuts at:      0     165     197    1668 
   Size:        165      32    1471

  Fragments arranged by size: 

                1471     165      32
 
PvuII CAG'CTG

Cuts at:      0      21    1425    1668 
   Size:         21    1404     243

  Fragments arranged by size: 

                1404     243      21
 
ScaI AGT'ACT

Cuts at:      0     583    1668 
   Size:        583    1085
 
SfcI C'TryA_G

Cuts at:      0     161     193    1668 
   Size:        161      32    1475

  Fragments arranged by size: 

                1475     161      32
 
SfoI GGC'GCC

Cuts at:      0     183    1668 
   Size:        183    1485
 
SmlI C'TyrA_G

Cuts at:      0     296    1668 
   Size:        296    1372
 
SphI G_CATG'C

Cuts at:      0     202    1158    1668 
   Size:        202     956     510

  Fragments arranged by size: 

                 956     510     202
 
StyI C'CwwG_G

Cuts at:      0     149     261    1339    1495    1668 
   Size:        149     112    1078     156     173

  Fragments arranged by size: 

                1078     173     156     149     112
 
TatI w'GTAC_w

Cuts at:      0     581    1241    1668 
   Size:        581     660     427

  Fragments arranged by size: 

                 660     581     427
 
Tth111I GACn'n_nGTC

Cuts at:      0      99    1668 
   Size:         99    1569
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     288     723    1668 
   Size:        288     435     945

  Fragments arranged by size: 

                 945     435     288
 
XhoI C'TCGA_G

Cuts at:      0     296    1668 
   Size:        296    1372
 

 Enzymes that do cut:

     AarI     AleI     ApaI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI    BfrBI     BglI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI    BstYI
   Bsu36I     BtgI     BtsI     DraI     EaeI     EarI     EciI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PsrI     PstI
    PvuII     ScaI     SfcI     SfoI     SmlI     SphI     StyI     TatI
  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BbvCI     BcgI     BclI    BglII     BlpI
    BmgBI     BmtI     BplI    BpuEI    BsaBI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     PacI     PasI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TstI     XbaI     XmaI     XmnI
     ZraI
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