Restriction map for pF1KB7740
 (Linear) (Six-base) MAPSORT of: KIBB7740.seq  Check: 2877  from: 1  to: 1446



KIBB7740.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     576    1446 
   Size:        576     870
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     465    1234    1446 
   Size:        281     184     769     212

  Fragments arranged by size: 

                 769     281     212     184
 
AlwNI CAG_nnn'CTG

Cuts at:      0     150    1446 
   Size:        150    1296
 
ApoI r'AATT_y

Cuts at:      0     629     845     859     876     928    1082    1446 
   Size:        629     216      14      17      52     154     364

  Fragments arranged by size: 

                 629     364     216     154      52      17      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     701     977    1446 
   Size:        701     276     469

  Fragments arranged by size: 

                 701     469     276
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     430    1446 
   Size:        430    1016
 
BmgBI CAC'GTC

Cuts at:      0    1440    1446 
   Size:       1440       6
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     288     842    1446 
   Size:        288     554     604

  Fragments arranged by size: 

                 604     554     288
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1190    1446 
   Size:       1190     256
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1148    1446 
   Size:       1148     298
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1183    1446 
   Size:       1183     263
 
BseYI C'CCAG_C

Cuts at:      0     251    1446 
   Size:        251    1195
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     887    1446 
   Size:        887     559
 
BsmI GAATG_Cn'

Cuts at:      0     619    1059    1446 
   Size:        619     440     387

  Fragments arranged by size: 

                 619     440     387
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     121    1446 
   Size:        121    1325
 
BsrDI GCAATG_nn'

Cuts at:      0     186    1446 
   Size:        186    1260
 
BsrGI T'GTAC_A

Cuts at:      0     509     548     912    1446 
   Size:        509      39     364     534

  Fragments arranged by size: 

                 534     509     364      39
 
BstBI TT'CG_AA

Cuts at:      0    1086    1446 
   Size:       1086     360
 
Bsu36I CC'TnA_GG

Cuts at:      0     827    1446 
   Size:        827     619
 
BtsI GCAGTG_nn'

Cuts at:      0      48    1446 
   Size:         48    1398
 
DraI TTT'AAA

Cuts at:      0     851    1446 
   Size:        851     595
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     738    1446 
   Size:        738     708
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     788    1446 
   Size:        257     531     658

  Fragments arranged by size: 

                 658     531     257
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     465    1190    1234    1446 
   Size:        281     184     725      44     212

  Fragments arranged by size: 

                 725     281     212     184      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     133    1446 
   Size:        133    1313
 
EcoRI G'AATT_C

Cuts at:      0     859     928    1082    1446 
   Size:        859      69     154     364

  Fragments arranged by size: 

                 859     364     154      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     415     447    1344    1376    1446 
   Size:        415      32     897      32      70

  Fragments arranged by size: 

                 897     415      70      32      32
 
HincII GTy'rAC

Cuts at:      0     577     623    1446 
   Size:        577      46     823

  Fragments arranged by size: 

                 823     577      46
 
HindIII A'AGCT_T

Cuts at:      0    1246    1446 
   Size:       1246     200
 
HpaI GTT'AAC

Cuts at:      0     623    1446 
   Size:        623     823
 
MfeI C'AATT_G

Cuts at:      0    1190    1446 
   Size:       1190     256
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     257    1446 
   Size:        257    1189
 
MslI CAynn'nnrTG

Cuts at:      0      82     446    1446 
   Size:         82     364    1000

  Fragments arranged by size: 

                1000     364      82
 
MspA1I CmG'CkG

Cuts at:      0      56     307    1027    1446 
   Size:         56     251     720     419

  Fragments arranged by size: 

                 720     419     251      56
 
NspI r_CATG'y

Cuts at:      0     555     676    1446 
   Size:        555     121     770

  Fragments arranged by size: 

                 770     555     121
 
PfoI T'CCnGG_A

Cuts at:      0     276    1446 
   Size:        276    1170
 
PpuMI rG'GwC_Cy

Cuts at:      0     133    1446 
   Size:        133    1313
 
PsiI TTA'TAA

Cuts at:      0    1136    1446 
   Size:       1136     310
 
PstI C_TGCA'G

Cuts at:      0      55    1446 
   Size:         55    1391
 
PvuII CAG'CTG

Cuts at:      0      56     307    1027    1446 
   Size:         56     251     720     419

  Fragments arranged by size: 

                 720     419     251      56
 
SalI G'TCGA_C

Cuts at:      0     575    1446 
   Size:        575     871
 
SfcI C'TryA_G

Cuts at:      0      51    1446 
   Size:         51    1395
 
SmlI C'TyrA_G

Cuts at:      0    1163    1446 
   Size:       1163     283
 
SphI G_CATG'C

Cuts at:      0     676    1446 
   Size:        676     770
 
TatI w'GTAC_w

Cuts at:      0     509     548     912    1446 
   Size:        509      39     364     534

  Fragments arranged by size: 

                 534     509     364      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     391     423    1446 
   Size:        391      32    1023

  Fragments arranged by size: 

                1023     391      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     878    1082    1446 
   Size:        878     204     364

  Fragments arranged by size: 

                 878     364     204
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     BbsI    BciVI    BmgBI     BplI
     BpmI    BpuEI    BseRI    BseYI     BsgI     BsmI    BspMI    BsrDI
    BsrGI    BstBI   Bsu36I     BtsI     DraI     DrdI     EarI  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI
     MslI   MspA1I     NspI     PfoI    PpuMI     PsiI     PstI    PvuII
     SalI     SfcI     SmlI     SphI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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