Restriction map for pF1KB7721
 (Linear) (Six-base) MAPSORT of: KIBB7721.seq  Check: 1697  from: 1  to: 1284



KIBB7721.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1117    1284 
   Size:       1117     167
 
AflIII A'CryG_T

Cuts at:      0     170     614    1284 
   Size:        170     444     670

  Fragments arranged by size: 

                 670     444     170
 
AleI CACnn'nnGTG

Cuts at:      0     518    1284 
   Size:        518     766
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1062    1284 
   Size:       1062     222
 
AvaI C'yCGr_G

Cuts at:      0     292     485    1284 
   Size:        292     193     799

  Fragments arranged by size: 

                 799     292     193
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1042    1075    1284 
   Size:       1042      33     209

  Fragments arranged by size: 

                1042     209      33
 
BanI G'GyrC_C

Cuts at:      0    1224    1284 
   Size:       1224      60
 
BanII G_rGCy'C

Cuts at:      0     299    1284 
   Size:        299     985
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     132    1284 
   Size:        132    1152
 
BbvCI CC'TCA_GC

Cuts at:      0     811    1284 
   Size:        811     473
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     782     816     976    1010    1017    1051    1284 
   Size:        782      34     160      34       7      34     233

  Fragments arranged by size: 

                 782     233     160      34      34      34       7
 
BglII A'GATC_T

Cuts at:      0     362    1284 
   Size:        362     922
 
Bme1580I G_kGCm'C

Cuts at:      0     665    1284 
   Size:        665     619
 
BmgBI CAC'GTC

Cuts at:      0      36    1137    1284 
   Size:         36    1101     147

  Fragments arranged by size: 

                1101     147      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     277     393    1284 
   Size:        277     116     891

  Fragments arranged by size: 

                 891     277     116
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     327    1284 
   Size:        327     957
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     745    1284 
   Size:        484     261     539

  Fragments arranged by size: 

                 539     484     261
 
Bpu10I CC'TnA_GC

Cuts at:      0     811    1284 
   Size:        811     473
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457    1284 
   Size:        457     827
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     124    1241    1284 
   Size:        124    1117      43

  Fragments arranged by size: 

                1117     124      43
 
BsaAI yAC'GTr

Cuts at:      0     749    1284 
   Size:        749     535
 
BsaBI GATnn'nnATC

Cuts at:      0     457    1284 
   Size:        457     827
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     142     172    1284 
   Size:        142      30    1112

  Fragments arranged by size: 

                1112     142      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     976    1201    1216    1284 
   Size:        976     225      15      68

  Fragments arranged by size: 

                 976     225      68      15
 
BseYI C'CCAG_C

Cuts at:      0     347     876    1284 
   Size:        347     529     408

  Fragments arranged by size: 

                 529     408     347
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382    1284 
   Size:        382     902
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     761    1284 
   Size:        494     267     523

  Fragments arranged by size: 

                 523     494     267
 
BsmI GAATG_Cn'

Cuts at:      0     838    1284 
   Size:        838     446
 
Bsp1286I G_dGCh'C

Cuts at:      0     299     494     665     761    1284 
   Size:        299     195     171      96     523

  Fragments arranged by size: 

                 523     299     195     171      96
 
BspHI T'CATG_A

Cuts at:      0     560    1131    1284 
   Size:        560     571     153

  Fragments arranged by size: 

                 571     560     153
 
BsrFI r'CCGG_y

Cuts at:      0     711    1284 
   Size:        711     573
 
BssSI C'ACGA_G

Cuts at:      0     871    1284 
   Size:        871     413
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12    1284 
   Size:         12    1272
 
BstYI r'GATC_y

Cuts at:      0     362    1070    1284 
   Size:        362     708     214

  Fragments arranged by size: 

                 708     362     214
 
Bsu36I CC'TnA_GG

Cuts at:      0    1199    1284 
   Size:       1199      85
 
BtgI C'CryG_G

Cuts at:      0      11     516    1284 
   Size:         11     505     768

  Fragments arranged by size: 

                 768     505      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      64     287     917    1284 
   Size:         64     223     630     367

  Fragments arranged by size: 

                 630     367     223      64
 
ClaI AT'CG_AT

Cuts at:      0     792    1284 
   Size:        792     492
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     735     770    1284 
   Size:        735      35     514

  Fragments arranged by size: 

                 735     514      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1120    1284 
   Size:       1120     164
 
EaeI y'GGCC_r

Cuts at:      0       2     639    1284 
   Size:          2     637     645

  Fragments arranged by size: 

                 645     637       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     941    1284 
   Size:        941     343
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1063    1284 
   Size:       1063     221
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     745    1117    1284 
   Size:        484     261     372     167

  Fragments arranged by size: 

                 484     372     261     167
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     605    1161    1284 
   Size:        605     556     123

  Fragments arranged by size: 

                 605     556     123
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1195    1284 
   Size:       1195      89
 
HincII GTy'rAC

Cuts at:      0     441    1284 
   Size:        441     843
 
HindIII A'AGCT_T

Cuts at:      0     536    1284 
   Size:        536     748
 
MscI TGG'CCA

Cuts at:      0       4    1284 
   Size:          4    1280
 
MslI CAynn'nnrTG

Cuts at:      0     229     518     565    1019    1256    1284 
   Size:        229     289      47     454     237      28

  Fragments arranged by size: 

                 454     289     237     229      47      28
 
MspA1I CmG'CkG

Cuts at:      0     351     914    1284 
   Size:        351     563     370

  Fragments arranged by size: 

                 563     370     351
 
NcoI C'CATG_G

Cuts at:      0     516    1284 
   Size:        516     768
 
NdeI CA'TA_TG

Cuts at:      0     924    1284 
   Size:        924     360
 
NspI r_CATG'y

Cuts at:      0     174     618    1284 
   Size:        174     444     666

  Fragments arranged by size: 

                 666     444     174
 
PciI A'CATG_T

Cuts at:      0     170     614    1284 
   Size:        170     444     670

  Fragments arranged by size: 

                 670     444     170
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      84     102     383     729    1062    1284 
   Size:         84      18     281     346     333     222

  Fragments arranged by size: 

                 346     333     281     222      84      18
 
PfoI T'CCnGG_A

Cuts at:      0     461    1284 
   Size:        461     823
 
PmlI CAC'GTG

Cuts at:      0     749    1284 
   Size:        749     535
 
PpuMI rG'GwC_Cy

Cuts at:      0    1195    1284 
   Size:       1195      89
 
PstI C_TGCA'G

Cuts at:      0     212    1146    1284 
   Size:        212     934     138

  Fragments arranged by size: 

                 934     212     138
 
PvuII CAG'CTG

Cuts at:      0     351    1284 
   Size:        351     933
 
ScaI AGT'ACT

Cuts at:      0     991    1284 
   Size:        991     293
 
SexAI A'CCwGG_T

Cuts at:      0     722    1284 
   Size:        722     562
 
SfcI C'TryA_G

Cuts at:      0     208     972    1142    1209    1284 
   Size:        208     764     170      67      75

  Fragments arranged by size: 

                 764     208     170      75      67
 
SmaI CCC'GGG

Cuts at:      0     294    1284 
   Size:        294     990
 
SmlI C'TyrA_G

Cuts at:      0     472    1284 
   Size:        472     812
 
StuI AGG'CCT

Cuts at:      0     157     421    1284 
   Size:        157     264     863

  Fragments arranged by size: 

                 863     264     157
 
StyI C'CwwG_G

Cuts at:      0     516    1284 
   Size:        516     768
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     868    1242    1284 
   Size:        868     374      42

  Fragments arranged by size: 

                 868     374      42
 
TatI w'GTAC_w

Cuts at:      0     989    1284 
   Size:        989     295
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     999    1031    1106    1138    1284 
   Size:        999      32      75      32     146

  Fragments arranged by size: 

                 999     146      75      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     556     567    1284 
   Size:        556      11     717

  Fragments arranged by size: 

                 717     556      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      55    1284 
   Size:         55    1229
 
XmaI C'CCGG_G

Cuts at:      0     292    1284 
   Size:        292     992
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     AvaI     BaeI     BanI    BanII
     BbsI    BbvCI     BcgI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrFI    BssSI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I   HincII  HindIII
     MscI     MslI   MspA1I     NcoI     NdeI     NspI     PciI    PflMI
     PfoI     PmlI    PpuMI     PstI    PvuII     ScaI    SexAI     SfcI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI     BbeI    BciVI     BclI    BfrBI     BglI
     BlpI     BmtI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII
  BstZ17I     BtsI     DraI     DrdI     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI
     XmnI     ZraI
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