Restriction map for
pF1KB7654
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FbaI FseI FspI HapII Hin1I
HincII HindII HindIII HpaI HpaII
KasI KspI MamI MfeI MluI
MroI MseI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SexAI SfoI SgrAI SmaI SnaBI
SphI SspBI SspI StuI SwaI
Tru9I Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 129 GGTACC G^GTACC
AflIII 456 ACGTGT A^C[GA][CT]GT
AlwI 270 GGATC GGATC(4/5)
AocI 946 CCTCAGG CC^T[ACGT]AGG
ApaI 95 GGGCCC GGGCC^C
Asp700I 442 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 129 GGTACC G^GTACC
AspHI 162 GTGCTC G[AT]GC[AT]^C
BalI 979 TGGCCA TGG^CCA
BanII 95 GGGCCC G[GA]GC[CT]^C
BglI 88 GCCGTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 484 AGATCT A^GATCT
BlpI 769 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 769 GCTGAGC GC^T[ACGT]AGC
BsgI 959 GTGCAG GTGCAG(16/14)
BsiHKAI 162 GTGCTC G[AT]GC[AT]^C
BstBI 473 TTCGAA TT^CGAA
BstEII 22 GGTGACC G^GT[ACGT]ACC
BstPI 22 GGTGACC G^GT[ACGT]ACC
Bsu36I 946 CCTCAGG CC^T[ACGT]AGG
CelII 769 GCTGAGC GC^T[ACGT]AGC
CfoI 112 GCGC GCG^C
EaeI 979 TGGCCA [CT]^GGCC[GA]
Eco81I 946 CCTCAGG CC^T[ACGT]AGG
EcoNI 45 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 22 GGTGACC G^GT[ACGT]ACC
EcoRV 657 GATATC GAT^ATC
EcoT14I 644 CCATGG C^C[AT][AT]GG
FauI 788 CCCGC CCCGC(4/6)
HaeII 111 GGCGCT [GA]GCGC^[CT]
HgaI 184 GCGTC GACGC(5/10)
HhaI 112 GCGC GCG^C
HinP1I 112 GCGC G^CGC
KpnI 129 GGTACC GGTAC^C
MaeII 456 ACGT A^CGT
MaeIII 23 GTGAC ^GT[ACGT]AC
MluNI 979 TGGCCA TGG^CCA
MscI 979 TGGCCA TGG^CCA
MslI 872 CACACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 644 CCATGG C^CATGG
NdeI 322 CATATG CA^TATG
NspI 941 ACATGC [GA]CATG^[CT]
NspV 473 TTCGAA TT^CGAA
SbfI 1032 CCTGCAGG CCTGCA^GG
ScaI 360 AGTACT AGT^ACT
SfiI 87 GGCCGTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 473 TTCGAA TT^CGAA
SmlI 491 CTCAAG C^T[CT][GA]AG
SpeI 702 ACTAGT A^CTAGT
Sse8387I 1032 CCTGCAGG CCTGCA^GG
StyI 644 CCATGG C^C[AT][AT]GG
TaqI 474 TCGA T^CGA
TfiI 471 GATTC G^A[AT]TC
Tsp45I 23 GTGAC ^GT[GC]AC
TthHB8I 474 TCGA T^CGA
XmnI 442 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 446 AAATTC [GA]^AATT[CT]
606 GAATTT
AlwNI 589 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
681 CAGCACCTG
ApoI 446 AAATTC [GA]^AATT[CT]
606 GAATTT
BanI 75 GGCACC G^G[CT][GA]CC
129 GGTACC
BstAPI 680 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
936 GCATTACATGC
BstXI 100 CCACTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
906 CCAGGCCCCTGG
BstYI 270 GGATCT [GA]^GATC[CT]
484 AGATCT
DsaI 250 CCACGG C^C[GA][CT]GG
644 CCATGG
EarI 994 CTCTTC CTCTTC(1/4)
378 GAAGAG
HphI 22 GGTGA GGTGA(8/7)
648 GGTGA
Ksp632I 994 CTCTTC CTCTTC(1/4)
378 GAAGAG
MflI 270 GGATCT [GA]^GATC[CT]
484 AGATCT
MspA1I 767 CAGCTG C[AC]G^C[GT]G
826 CAGCTG
PstI 844 CTGCAG CTGCA^G
1033 CTGCAG
PvuII 767 CAGCTG CAG^CTG
826 CAGCTG
SfaNI 16 GATGC GCATC(5/9)
689 GCATC
XbaI 273 TCTAGA T^CTAGA
618 TCTAGA
XhoII 270 GGATCT [GA]^GATC[CT]
484 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeI MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp509I TspRI Van91I