Restriction map for pF1KB7631
 (Linear) (Six-base) MAPSORT of: KIBB7631.seq  Check: 3557  from: 1  to: 960



KIBB7631.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0      32     960 
   Size:         32     928
 
AfeI AGC'GCT

Cuts at:      0     400     960 
   Size:        400     560
 
AflIII A'CryG_T

Cuts at:      0     593     960 
   Size:        593     367
 
AhdI GACnn_n'nnGTC

Cuts at:      0     753     960 
   Size:        753     207
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     869     938     960 
   Size:        869      69      22

  Fragments arranged by size: 

                 869      69      22
 
AlwNI CAG_nnn'CTG

Cuts at:      0     486     960 
   Size:        486     474
 
ApaI G_GGCC'C

Cuts at:      0     547     960 
   Size:        547     413
 
ApaLI G'TGCA_C

Cuts at:      0     205     960 
   Size:        205     755
 
AvaI C'yCGr_G

Cuts at:      0     216     555     648     718     960 
   Size:        216     339      93      70     242

  Fragments arranged by size: 

                 339     242     216      93      70
 
BanI G'GyrC_C

Cuts at:      0     257     356     517     655     682     699     795     960 
   Size:        257      99     161     138      27      17      96     165

  Fragments arranged by size: 

                 257     165     161     138      99      96      27      17
            
 
BanII G_rGCy'C

Cuts at:      0     547     790     960 
   Size:        547     243     170

  Fragments arranged by size: 

                 547     243     170
 
BbeI G_GCGC'C

Cuts at:      0     360     521     659     686     703     799     960 
   Size:        360     161     138      27      17      96     161

  Fragments arranged by size: 

                 360     161     161     138      96      27      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     137     960 
   Size:        137     823
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     772     806     960 
   Size:        772      34     154

  Fragments arranged by size: 

                 772     154      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     255     295     566     960 
   Size:        255      40     271     394

  Fragments arranged by size: 

                 394     271     255      40
 
Bme1580I G_kGCm'C

Cuts at:      0     209     260     547     922     960 
   Size:        209      51     287     375      38

  Fragments arranged by size: 

                 375     287     209      51      38
 
BmgBI CAC'GTC

Cuts at:      0     407     819     960 
   Size:        407     412     141

  Fragments arranged by size: 

                 412     407     141
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     888     960 
   Size:        888      72
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     792     960 
   Size:        792     168
 
Bpu10I CC'TnA_GC

Cuts at:      0     364     960 
   Size:        364     596
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     960 
   Size:        379     581
 
BsaAI yAC'GTr

Cuts at:      0     156     226     960 
   Size:        156      70     734

  Fragments arranged by size: 

                 734     156      70
 
BsaHI Gr'CG_yC

Cuts at:      0     357     503     518     656     683     700     796     905 
   Size:        357     146      15     138      27      17      96     109

Cuts at:    905     960 
   Size:         55

  Fragments arranged by size: 

                 357     146     138     109      96      55      27      17
                  15
 
BsaWI w'CCGG_w

Cuts at:      0     900     960 
   Size:        900      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     960 
   Size:         25     935
 
BseYI C'CCAG_C

Cuts at:      0     250     703     862     960 
   Size:        250     453     159      98

  Fragments arranged by size: 

                 453     250     159      98
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387     960 
   Size:        387     573
 
BsiEI CG_ry'CG

Cuts at:      0     327     960 
   Size:        327     633
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     209     575     959     960 
   Size:        185      24     366     384       1

  Fragments arranged by size: 

                 384     366     185      24       1
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     209     260     547     575     790     922     959 
   Size:        185      24      51     287      28     215     132      37

Cuts at:    959     960 
   Size:          1

  Fragments arranged by size: 

                 287     215     185     132      51      37      28      24
                   1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     960 
   Size:        125     835
 
BsrBI CCG'CTC

Cuts at:      0      21     354     653     960 
   Size:         21     333     299     307

  Fragments arranged by size: 

                 333     307     299      21
 
BssHII G'CGCG_C

Cuts at:      0     161     243     245     960 
   Size:        161      82       2     715

  Fragments arranged by size: 

                 715     161      82       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     299     486     960 
   Size:        299     187     474

  Fragments arranged by size: 

                 474     299     187
 
BstBI TT'CG_AA

Cuts at:      0     128     960 
   Size:        128     832
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     765     960 
   Size:        765     195
 
BstYI r'GATC_y

Cuts at:      0     443     960 
   Size:        443     517
 
BtgI C'CryG_G

Cuts at:      0     197     561     960 
   Size:        197     364     399

  Fragments arranged by size: 

                 399     364     197
 
BtsI GCAGTG_nn'

Cuts at:      0     922     960 
   Size:        922      38
 
EarI CTCTTCn'nnn_

Cuts at:      0     173     718     960 
   Size:        173     545     242

  Fragments arranged by size: 

                 545     242     173
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     681     829     960 
   Size:        681     148     131

  Fragments arranged by size: 

                 681     148     131
 
EcoO109I rG'GnC_Cy

Cuts at:      0     474     960 
   Size:        474     486
 
FspI TGC'GCA

Cuts at:      0     451     960 
   Size:        451     509
 
HaeII r_GCGC'y

Cuts at:      0     360     402     521     659     686     703     799     960 
   Size:        360      42     119     138      27      17      96     161

  Fragments arranged by size: 

                 360     161     138     119      96      42      27      17
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     739     771     960 
   Size:         27     712      32     189

  Fragments arranged by size: 

                 712     189      32      27
 
KasI G'GCGC_C

Cuts at:      0     356     517     655     682     699     795     960 
   Size:        356     161     138      27      17      96     165

  Fragments arranged by size: 

                 356     165     161     138      96      27      17
 
MluI A'CGCG_T

Cuts at:      0     593     960 
   Size:        593     367
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     635     644     960 
   Size:        614      21       9     316

  Fragments arranged by size: 

                 614     316      21       9
 
MspA1I CmG'CkG

Cuts at:      0     199     254     275     426     549     563     581     641 
   Size:        199      55      21     151     123      14      18      60

Cuts at:    641     881     960 
   Size:        240      79

  Fragments arranged by size: 

                 240     199     151     123      79      60      55      21
                  18      14
 
NarI GG'CG_CC

Cuts at:      0     357     518     656     683     700     796     960 
   Size:        357     161     138      27      17      96     164

  Fragments arranged by size: 

                 357     164     161     138      96      27      17
 
NspI r_CATG'y

Cuts at:      0     241     960 
   Size:        241     719
 
PmlI CAC'GTG

Cuts at:      0     156     226     960 
   Size:        156      70     734

  Fragments arranged by size: 

                 734     156      70
 
PspOMI G'GGCC_C

Cuts at:      0     543     960 
   Size:        543     417
 
PstI C_TGCA'G

Cuts at:      0     482     960 
   Size:        482     478
 
SacII CC_GC'GG

Cuts at:      0     200     564     960 
   Size:        200     364     396

  Fragments arranged by size: 

                 396     364     200
 
SapI GCTCTTCn'nnn_

Cuts at:      0     173     960 
   Size:        173     787
 
SbfI CC_TGCA'GG

Cuts at:      0     482     960 
   Size:        482     478
 
SexAI A'CCwGG_T

Cuts at:      0     950     960 
   Size:        950      10
 
SfcI C'TryA_G

Cuts at:      0     478     576     636     960 
   Size:        478      98      60     324

  Fragments arranged by size: 

                 478     324      98      60
 
SfoI GGC'GCC

Cuts at:      0     358     519     657     684     701     797     960 
   Size:        358     161     138      27      17      96     163

  Fragments arranged by size: 

                 358     163     161     138      96      27      17
 
SmaI CCC'GGG

Cuts at:      0     218     557     720     960 
   Size:        218     339     163     240

  Fragments arranged by size: 

                 339     240     218     163
 
SmlI C'TyrA_G

Cuts at:      0     394     960 
   Size:        394     566
 
StuI AGG'CCT

Cuts at:      0     382     960 
   Size:        382     578
 
StyI C'CwwG_G

Cuts at:      0     434     960 
   Size:        434     526
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     907     960 
   Size:        907      53
 
XmaI C'CCGG_G

Cuts at:      0     216     555     718     960 
   Size:        216     339     163     242

  Fragments arranged by size: 

                 339     242     216     163
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AhdI     AlfI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BglI Bme1580I
    BmgBI     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI
   BssHII   BstAPI    BstBI    BstXI    BstYI     BtgI     BtsI     EarI
     EciI EcoO109I     FspI    HaeII    Hin4I     KasI     MluI     MmeI
   MspA1I     NarI     NspI     PmlI   PspOMI     PstI    SacII     SapI
     SbfI    SexAI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AleI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BpmI
     BsaI    BsaBI    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI    BssSI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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