Restriction map for pF1KB7619
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# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MfeI        MluI        MroI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   29 enzymes) #
###################################

BalI          410  TGGCCA               TGG^CCA
BamHI         403  GGATCC               G^GATCC
BanI          648  GGTGCC               G^G[CT][GA]CC
BanII         601  GGGCTC               G[GA]GC[CT]^C
BbsI          502  GAAGAC               GAAGAC(2/6)
BbvCI         314  CCTCAGC              CCTCAGC(-5/-2)
BmyI          601  GGGCTC               G[AGT]GC[ACT]^C
BpmI          881  CTCCAG               CTGGAG(16/14)
BpuAI         502  GAAGAC               GAAGAC(2/6)
BsaAI         849  TACGTG               [CT]AC^GT[GA]
BsaBI         700  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          248  GAGACC               GGTCTC(1/5)
BseRI         604  CTCCTC               GAGGAG(10/8)
Bsp1286I      601  GGGCTC               G[AGT]GC[ACT]^C
BstAPI        672  GCAGTATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          55  CCAATTTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          865  TTTAAA               TTT^AAA
EaeI          410  TGGCCA               [CT]^GGCC[GA]
EcoNI         606  CCTCCAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       108  ATGCAT               ATGCA^T
HaeIII        411  GGCC                 GG^CC
HphI          486  GGTGA                GGTGA(8/7)
MaeII         850  ACGT                 A^CGT
MamI          700  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         410  TGGCCA               TGG^CCA
MscI          410  TGGCCA               TGG^CCA
NsiI          108  ATGCAT               ATGCA^T
TaqI          774  TCGA                 T^CGA
TthHB8I       774  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          639  GTAC                 GT^AC
              845  GTAC                
AlwI          404  GATCC                GGATC(4/5)
              403  GGATC               
BglII         348  AGATCT               A^GATCT
              712  AGATCT              
BsaJI         252  CCCTGG               C^C[ACGT][ACGT]GG
              407  CCCTGG              
BsiYI         593  CCGACTCAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              606  CCTCCAACAGG         
BslI          593  CCGACTCAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              606  CCTCCAACAGG         
BspHI         149  TCATGA               T^CATGA
              530  TCATGA              
BsrI          736  CCAGT                ACTGG(1/-1)
              883  CCAGT               
EarI          665  CTCTTC               CTCTTC(1/4)
              337  GAAGAG              
HincII        377  GTCAAC               GT[CT]^[GA]AC
              745  GTTGAC              
HindII        377  GTCAAC               GT[CT]^[GA]AC
              745  GTTGAC              
Ksp632I       665  CTCTTC               CTCTTC(1/4)
              337  GAAGAG              
PleI          589  GACTC                GAGTC(4/5)
              595  GACTC               
PstI          497  CTGCAG               CTGCA^G
              670  CTGCAG              
RcaI          149  TCATGA               T^CATGA
              530  TCATGA              
RsaI          639  GTAC                 GT^AC
              845  GTAC                
SspI          562  AATATT               AAT^ATT
              819  AATATT              
TspRI         507  CAGTG                CA[GC]TG[ACGT][ACGT]^
              884  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AcsI        ApoI        BbvI        BsmAI       BssKI       
BstNI       BstYI       Cac8I       DdeI        DpnII       
EcoRII      Fnu4HI      HinfI       ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      ScrFI       SfaNI       SfcI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     XhoII       
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