Restriction map for pF1KB7611
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApoI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BciVI       BclI        BfrI        BglII       BlnI        
BpmI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HindIII     HpaI        KpnI        MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        NaeI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        Sau3AI      
ScaI        SexAI       SfaNI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       Tth111I     XbaI        XcmI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          402  GGCGCC               G[GA]^CG[CT]C
Alw44I        844  GTGCAC               G^TGCAC
ApaLI         844  GTGCAC               G^TGCAC
AscI          338  GGCGCGCC             GG^CGCGCC
BalI          791  TGGCCA               TGG^CCA
BbeI          402  GGCGCC               GGCGC^C
BbsI          559  GTCTTC               GAAGAC(2/6)
BcgI          276  CGAGGAGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          229  CTAG                 C^TAG
BglI          733  GCCGCCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           15  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       15  GCTCAGC              GC^T[ACGT]AGC
BpuAI         559  GTCTTC               GAAGAC(2/6)
BsaHI         402  GGCGCC               G[GA]^CG[CT]C
BsiWI         455  CGTACG               C^GTACG
BsmFI         770  GGGAC                GGGAC(10/14)
BsrFI         335  ACCGGC               [GA]^CCGG[CT]
BssHII        339  GCGCGC               G^CGCGC
CelII          15  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        335  ACCGGC               [GA]^CCGG[CT]
EarI          750  CTCTTC               CTCTTC(1/4)
HaeII         402  GGCGCC               [GA]GCGC^[CT]
HgaI           58  GACGC                GACGC(5/10)
Hin1I         402  GGCGCC               G[GA]^CG[CT]C
HincII        529  GTCAAC               GT[CT]^[GA]AC
HindII        529  GTCAAC               GT[CT]^[GA]AC
HinfI         118  GACTC                G^A[ACGT]TC
KasI          402  GGCGCC               G^GCGCC
Ksp632I       750  CTCTTC               CTCTTC(1/4)
MaeI          229  CTAG                 C^TAG
MaeIII        779  GTAAC                ^GT[ACGT]AC
MluNI         791  TGGCCA               TGG^CCA
MscI          791  TGGCCA               TGG^CCA
NarI          402  GGCGCC               GG^CGCC
NcoI          590  CCATGG               C^CATGG
PaeR7I        783  CTCGAG               C^TCGAG
PflMI         628  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          118  GACTC                GAGTC(4/5)
PpuMI         770  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          643  CTGCAG               CTGCA^G
SbfI          642  CCTGCAGG             CCTGCA^GG
SfoI          402  GGCGCC               GGC^GCC
SmlI          783  CTCGAG               C^T[CT][GA]AG
Sse8387I      642  CCTGCAGG             CCTGCA^GG
StuI          128  AGGCCT               AGG^CCT
TaqI          784  TCGA                 T^CGA
TthHB8I       784  TCGA                 T^CGA
Van91I        628  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          783  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

ApaI          638  GGGCCC               GGGCC^C
              660  GGGCCC              
AvaII         293  GGACC                G^G[AT]CC
              771  GGACC               
BseRI         277  GAGGAG               GAGGAG(10/8)
              433  GAGGAG              
BsiEI         680  CGGCCG               CG[GA][CT]^CG
              722  CGGCCG              
BsrBI         406  CCGCTC               CCGCTC(-3/-3)
              490  CCGCTC              
BsrI          464  ACTGG                ACTGG(1/-1)
              882  CCAGT               
EagI          680  CGGCCG               C^GGCCG
              722  CGGCCG              
EclXI         680  CGGCCG               C^GGCCG
              722  CGGCCG              
Eco52I        680  CGGCCG               C^GGCCG
              722  CGGCCG              
EcoNI         239  CCTTCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              300  CCTCACCGAGG         
EcoT14I       473  CCAAGG               C^C[AT][AT]GG
              590  CCATGG              
HphI          206  TCACC                GGTGA(8/7)
              302  TCACC               
KspI          605  CCGCGG               CCGC^GG
              737  CCGCGG              
MaeII         533  ACGT                 A^CGT
              761  ACGT                
MboII         560  TCTTC                GAAGA(8/7)
              751  TCTTC               
NlaIII        264  CATG                 CATG^
              591  CATG                
SacII         605  CCGCGG               CCGC^GG
              737  CCGCGG              
SfcI          220  CTACAG               C^T[GA][CT]AG
              643  CTGCAG              
SmaI          615  CCCGGG               CCC^GGG
              667  CCCGGG              
StyI          473  CCAAGG               C^C[AT][AT]GG
              590  CCATGG              
XmaCI         615  CCCGGG               C^CCGGG
              667  CCCGGG              
XmaI          615  CCCGGG               C^CCGGG
              667  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaI        BanI        BanII       BbvI        
BcnI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsoBI       Bsp1286I    BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIV       RsaI        Sau96I      ScrFI       TseI        
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