Restriction map for pF1KB7595
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstF5I      
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HgaI        HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MseI        MunI        MvnI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          676  GTCTAC               GT^[AC][GT]AC
AcyI          127  GGCGCC               G[GA]^CG[CT]C
AfaI          393  GTAC                 GT^AC
AlwNI         307  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       625  AGCGCT               AGC^GCT
ApaI           67  GGGCCC               GGGCC^C
AvaI          249  CCCGAG               C^[CT]CG[GA]G
BalI          338  TGGCCA               TGG^CCA
BbeI          127  GGCGCC               GGCGC^C
BsaHI         127  GGCGCC               G[GA]^CG[CT]C
BsaI          538  GAGACC               GGTCTC(1/5)
BsgI          151  CTGCAC               GTGCAG(16/14)
BsmBI         480  CGTCTC               CGTCTC(1/5)
BsoBI         249  CCCGAG               C^[CT]CG[GA]G
BspMI         672  GCAGGT               ACCTGC(4/8)
BsrDI         690  CATTGC               GCAATG(2/0)
BsrI          306  CCAGT                ACTGG(1/-1)
BstEII        613  GGTGACC              G^GT[ACGT]ACC
BstPI         613  GGTGACC              G^GT[ACGT]ACC
Eco47III      625  AGCGCT               AGC^GCT
EcoO65I       613  GGTGACC              G^GT[ACGT]ACC
Hin1I         127  GGCGCC               G[GA]^CG[CT]C
HincII        499  GTCAAC               GT[CT]^[GA]AC
HindII        499  GTCAAC               GT[CT]^[GA]AC
KasI          127  GGCGCC               G^GCGCC
MaeII          26  ACGT                 A^CGT
MluNI         338  TGGCCA               TGG^CCA
MscI          338  TGGCCA               TGG^CCA
MslI          441  CACCCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          127  GGCGCC               GG^CGCC
PshAI         492  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          393  GTAC                 GT^AC
SapI          138  GCTCTTC              GCTCTTC(1/4)
SfaNI         473  GCATC                GCATC(5/9)
SfcI          263  CTGTAG               C^T[GA][CT]AG
SfoI          127  GGCGCC               GGC^GCC
SmlI          287  CTCAAG               C^T[CT][GA]AG
XcmI          326  CCAATGCCCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AlwI           18  GATCC                GGATC(4/5)
              686  GGATC               
AspHI           3  GAGCAC               G[AT]GC[AT]^C
              448  GTGCTC              
AvaII         195  GGACC                G^G[AT]CC
              411  GGTCC               
BanI          127  GGCGCC               G^G[CT][GA]CC
              375  GGTGCC              
BbsI           57  GAAGAC               GAAGAC(2/6)
              595  GTCTTC              
BcnI           21  CCGGG                CC^[GC]GG
              321  CCGGG               
BglI           37  GCCGAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              331  GCCCTCCTGGC         
BpuAI          57  GAAGAC               GAAGAC(2/6)
              595  GTCTTC              
BsiHKAI         3  GAGCAC               G[AT]GC[AT]^C
              448  GTGCTC              
BsmAI         538  GAGAC                GTCTC(1/5)
              481  GTCTC               
BsmFI          23  GGGAC                GGGAC(10/14)
              194  GGGAC               
BstXI         341  CCAATGGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              578  CCATCTATCTGG        
EaeI           35  TGGCCG               [CT]^GGCC[GA]
              338  TGGCCA              
EcoNI         414  CCTCTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              527  CCTGCCAGAGG         
EcoT14I       426  CCAAGG               C^C[AT][AT]GG
              494  CCAAGG              
HapII          21  CCGG                 C^CGG
              321  CCGG                
HinfI         620  GACTC                G^A[ACGT]TC
              664  GAGTC               
HpaII          21  CCGG                 C^CGG
              321  CCGG                
HphI          613  GGTGA                GGTGA(8/7)
              455  TCACC               
MaeIII        254  GTGAC                ^GT[ACGT]AC
              614  GTGAC               
MspA1I        367  CAGCTG               C[AC]G^C[GT]G
              601  CAGCTG              
MspI           21  CCGG                 C^CGG
              321  CCGG                
NciI           21  CCGGG                CC^[GC]GG
              321  CCGGG               
PflMI         366  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              560  CCAAGCCCTGG         
PleI          620  GACTC                GAGTC(4/5)
              664  GAGTC               
PpuMI         194  GGGACCT              [GA]G^G[AT]CC[CT]
              410  AGGTCCT             
PvuII         367  CAGCTG               CAG^CTG
              601  CAGCTG              
StyI          426  CCAAGG               C^C[AT][AT]GG
              494  CCAAGG              
TaqI          243  TCGA                 T^CGA
              653  TCGA                
Tsp45I        254  GTGAC                ^GT[GC]AC
              614  GTGAC               
TspRI           6  CACTG                CA[GC]TG[ACGT][ACGT]^
              307  CAGTG               
TthHB8I       243  TCGA                 T^CGA
              653  TCGA                
Van91I        366  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              560  CCAAGCCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        BanII       BbvCI       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      Fnu4HI      HaeII       
HaeIII      HhaI        HinP1I      ItaI        Ksp632I     
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       TseI        
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