Restriction map for pF1KB7595
 (Linear) (Six-base) MAPSORT of: KIBB7595.seq  Check: 1281  from: 1  to: 699



KIBB7595.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     677     699 
   Size:        677      22
 
AfeI AGC'GCT

Cuts at:      0     627     699 
   Size:        627      72
 
AleI CACnn'nnGTG

Cuts at:      0     445     699 
   Size:        445     254
 
AlwNI CAG_nnn'CTG

Cuts at:      0     312     699 
   Size:        312     387
 
ApaI G_GGCC'C

Cuts at:      0      71     699 
   Size:         71     628
 
AvaI C'yCGr_G

Cuts at:      0     249     699 
   Size:        249     450
 
BanI G'GyrC_C

Cuts at:      0     127     375     699 
   Size:        127     248     324

  Fragments arranged by size: 

                 324     248     127
 
BanII G_rGCy'C

Cuts at:      0      71      79     526     578     699 
   Size:         71       8     447      52     121

  Fragments arranged by size: 

                 447     121      71      52       8
 
BbeI G_GCGC'C

Cuts at:      0     131     699 
   Size:        131     568
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      64     588     699 
   Size:         64     524     111

  Fragments arranged by size: 

                 524     111      64
 
BbvCI CC'TCA_GC

Cuts at:      0      97     293     554     699 
   Size:         97     196     261     145

  Fragments arranged by size: 

                 261     196     145      97
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43     337     699 
   Size:         43     294     362

  Fragments arranged by size: 

                 362     294      43
 
Bme1580I G_kGCm'C

Cuts at:      0      71     699 
   Size:         71     628
 
BmgBI CAC'GTC

Cuts at:      0      27     699 
   Size:         27     672
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     289     625     699 
   Size:         63     226     336      74

  Fragments arranged by size: 

                 336     226      74      63
 
Bpu10I CC'TnA_GC

Cuts at:      0      97     293     554     699 
   Size:         97     196     261     145

  Fragments arranged by size: 

                 261     196     145      97
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     699 
   Size:        272     427
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     532     699 
   Size:        532     167
 
BsaHI Gr'CG_yC

Cuts at:      0     128     699 
   Size:        128     571
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     442     496     699 
   Size:         55     387      54     203

  Fragments arranged by size: 

                 387     203      55      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     699 
   Size:        136     563
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     452     699 
   Size:          7     445     247

  Fragments arranged by size: 

                 445     247       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     486     699 
   Size:        486     213
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      71      79     452     526     578     699 
   Size:          7      64       8     373      74      52     121

  Fragments arranged by size: 

                 373     121      74      64      52       8       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     663     699 
   Size:        663      36
 
BsrDI GCAATG_nn'

Cuts at:      0     689     699 
   Size:        689      10
 
BstEII G'GTnAC_C

Cuts at:      0     613     699 
   Size:        613      86
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     348     585     699 
   Size:        348     237     114

  Fragments arranged by size: 

                 348     237     114
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     459     699 
   Size:        459     240
 
EaeI y'GGCC_r

Cuts at:      0      35     338     699 
   Size:         35     303     361

  Fragments arranged by size: 

                 361     303      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     109     145     176     421     699 
   Size:        109      36      31     245     278

  Fragments arranged by size: 

                 278     245     109      36      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     289     625     699 
   Size:         63     226     336      74

  Fragments arranged by size: 

                 336     226      74      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     418     531     699 
   Size:        418     113     168

  Fragments arranged by size: 

                 418     168     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0      67      68     195     387     411     699 
   Size:         67       1     127     192      24     288

  Fragments arranged by size: 

                 288     192     127      67      24       1
 
HaeII r_GCGC'y

Cuts at:      0      49     131     629     699 
   Size:         49      82     498      70

  Fragments arranged by size: 

                 498      82      70      49
 
HincII GTy'rAC

Cuts at:      0     501     699 
   Size:        501     198
 
KasI G'GCGC_C

Cuts at:      0     127     699 
   Size:        127     572
 
MscI TGG'CCA

Cuts at:      0     340     699 
   Size:        340     359
 
MslI CAynn'nnrTG

Cuts at:      0     445     699 
   Size:        445     254
 
MspA1I CmG'CkG

Cuts at:      0     369     603     699 
   Size:        369     234      96

  Fragments arranged by size: 

                 369     234      96
 
NarI GG'CG_CC

Cuts at:      0     128     699 
   Size:        128     571
 
PasI CC'CwG_GG

Cuts at:      0      72     191     699 
   Size:         72     119     508

  Fragments arranged by size: 

                 508     119      72
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     372     566     699 
   Size:        372     194     133

  Fragments arranged by size: 

                 372     194     133
 
PfoI T'CCnGG_A

Cuts at:      0      20     699 
   Size:         20     679
 
PpuMI rG'GwC_Cy

Cuts at:      0     195     411     699 
   Size:        195     216     288

  Fragments arranged by size: 

                 288     216     195
 
PshAI GACnn'nnGTC

Cuts at:      0     496     699 
   Size:        496     203
 
PspOMI G'GGCC_C

Cuts at:      0      67     699 
   Size:         67     632
 
PvuII CAG'CTG

Cuts at:      0     369     603     699 
   Size:        369     234      96

  Fragments arranged by size: 

                 369     234      96
 
SapI GCTCTTCn'nnn_

Cuts at:      0     145     699 
   Size:        145     554
 
SfcI C'TryA_G

Cuts at:      0     263     699 
   Size:        263     436
 
SfoI GGC'GCC

Cuts at:      0     129     699 
   Size:        129     570
 
SmlI C'TyrA_G

Cuts at:      0     287     699 
   Size:        287     412
 
StyI C'CwwG_G

Cuts at:      0     426     494     699 
   Size:        426      68     205

  Fragments arranged by size: 

                 426     205      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     457     472     632     699 
   Size:        457      15     160      67

  Fragments arranged by size: 

                 457     160      67      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     333     699 
   Size:        333     366
 

 Enzymes that do cut:

     AccI     AfeI     AleI    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI Bme1580I    BmgBI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrDI   BstEII    BstXI    BtgZI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    HaeII   HincII     KasI     MscI     MslI   MspA1I
     NarI     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI    PvuII
     SapI     SfcI     SfoI     SmlI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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