Restriction map for pF1KB7587
 (Linear) (Six-base) MAPSORT of: KIBB7587.seq  Check: 9851  from: 1  to: 672



KIBB7587.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     672 
   Size:        331     341
 
AlwNI CAG_nnn'CTG

Cuts at:      0     195     345     402     672 
   Size:        195     150      57     270

  Fragments arranged by size: 

                 270     195     150      57
 
ApaI G_GGCC'C

Cuts at:      0     146     461     672 
   Size:        146     315     211

  Fragments arranged by size: 

                 315     211     146
 
ApaLI G'TGCA_C

Cuts at:      0     524     672 
   Size:        524     148
 
AvaI C'yCGr_G

Cuts at:      0     130     172     672 
   Size:        130      42     500

  Fragments arranged by size: 

                 500     130      42
 
BanI G'GyrC_C

Cuts at:      0     471     672 
   Size:        471     201
 
BanII G_rGCy'C

Cuts at:      0     119     130     146     461     672 
   Size:        119      11      16     315     211

  Fragments arranged by size: 

                 315     211     119      16      11
 
BglI GCCn_nnn'nGGC

Cuts at:      0     304     672 
   Size:        304     368
 
BlpI GC'TnA_GC

Cuts at:      0     406     672 
   Size:        406     266
 
Bme1580I G_kGCm'C

Cuts at:      0     146     461     476     528     672 
   Size:        146     315      15      52     144

  Fragments arranged by size: 

                 315     146     144      52      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     379     550     672 
   Size:         64     315     171     122

  Fragments arranged by size: 

                 315     171     122      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     124     672 
   Size:        124     548
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     265     672 
   Size:        169      96     407

  Fragments arranged by size: 

                 407     169      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     484     514     580     610     672 
   Size:        484      30      66      30      62

  Fragments arranged by size: 

                 484      66      62      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439     672 
   Size:        439     233
 
BseYI C'CCAG_C

Cuts at:      0     162     461     475     672 
   Size:        162     299      14     197

  Fragments arranged by size: 

                 299     197     162      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     672 
   Size:        577      95
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     179     528     672 
   Size:        119      60     349     144

  Fragments arranged by size: 

                 349     144     119      60
 
BsmI GAATG_Cn'

Cuts at:      0     487     672 
   Size:        487     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     130     146     179     461     476     528     672 
   Size:        119      11      16      33     282      15      52     144

  Fragments arranged by size: 

                 282     144     119      52      33      16      15      11
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     599     672 
   Size:         74     525      73

  Fragments arranged by size: 

                 525      74      73
 
BsrBI CCG'CTC

Cuts at:      0     108     672 
   Size:        108     564
 
BsrFI r'CCGG_y

Cuts at:      0     242     672 
   Size:        242     430
 
BstBI TT'CG_AA

Cuts at:      0      89     672 
   Size:         89     583
 
BstYI r'GATC_y

Cuts at:      0     359     672 
   Size:        359     313
 
BtgI C'CryG_G

Cuts at:      0     199     236     672 
   Size:        199      37     436

  Fragments arranged by size: 

                 436     199      37
 
BtsI GCAGTG_nn'

Cuts at:      0     375     528     672 
   Size:        375     153     144

  Fragments arranged by size: 

                 375     153     144
 
EaeI y'GGCC_r

Cuts at:      0     254     672 
   Size:        254     418
 
EarI CTCTTCn'nnn_

Cuts at:      0     307     672 
   Size:        307     365
 
EcoICRI GAG'CTC

Cuts at:      0     117     672 
   Size:        117     555
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     331     379     550     672 
   Size:         64     267      48     171     122

  Fragments arranged by size: 

                 267     171     122      64      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     418     672 
   Size:        418     254
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135     142     143     167     399     458     672 
   Size:        135       7       1      24     232      59     214

  Fragments arranged by size: 

                 232     214     135      59      24       7       1
 
FspI TGC'GCA

Cuts at:      0     367     672 
   Size:        367     305
 
HaeII r_GCGC'y

Cuts at:      0     393     500     542     672 
   Size:        393     107      42     130

  Fragments arranged by size: 

                 393     130     107      42
 
MslI CAynn'nnrTG

Cuts at:      0     625     672 
   Size:        625      47
 
MspA1I CmG'CkG

Cuts at:      0     342     672 
   Size:        342     330
 
PasI CC'CwG_GG

Cuts at:      0     139     547     672 
   Size:        139     408     125

  Fragments arranged by size: 

                 408     139     125
 
PpuMI rG'GwC_Cy

Cuts at:      0     167     672 
   Size:        167     505
 
PspOMI G'GGCC_C

Cuts at:      0     142     457     672 
   Size:        142     315     215

  Fragments arranged by size: 

                 315     215     142
 
PstI C_TGCA'G

Cuts at:      0     508     672 
   Size:        508     164
 
SacI G_AGCT'C

Cuts at:      0     119     672 
   Size:        119     553
 
SanDI GG'GwC_CC

Cuts at:      0     167     672 
   Size:        167     505
 
SfcI C'TryA_G

Cuts at:      0     504     575     672 
   Size:        504      71      97

  Fragments arranged by size: 

                 504      97      71
 
SmlI C'TyrA_G

Cuts at:      0     148     280     672 
   Size:        148     132     392

  Fragments arranged by size: 

                 392     148     132
 
StuI AGG'CCT

Cuts at:      0     298     672 
   Size:        298     374
 
StyI C'CwwG_G

Cuts at:      0     350     672 
   Size:        350     322
 
TatI w'GTAC_w

Cuts at:      0     380     672 
   Size:        380     292
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     484     516     672 
   Size:        484      32     156

  Fragments arranged by size: 

                 484     156      32
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BglI
     BlpI Bme1580I     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI    BstBI
    BstYI     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FspI    HaeII     MslI   MspA1I     PasI    PpuMI   PspOMI
     PstI     SacI    SanDI     SfcI     SmlI     StuI     StyI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoRI    EcoRV     FalI     FseI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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