Restriction map for pF1KB7569
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BpmI        BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DsaI        
Eam1105I    EarI        Eco47III    EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
MwoI        NaeI        NarI        NcoI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AocI          388  CCTAAGG              CC^T[ACGT]AGG
AspHI         169  GAGCTC               G[AT]GC[AT]^C
AvaII          78  GGACC                G^G[AT]CC
BanI          427  GGCACC               G^G[CT][GA]CC
BanII         169  GAGCTC               G[GA]GC[CT]^C
BbsI          214  GAAGAC               GAAGAC(2/6)
BlpI          377  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      377  GCTCAGC              GC^T[ACGT]AGC
BpuAI         214  GAAGAC               GAAGAC(2/6)
BsaBI         315  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         343  GAGGAG               GAGGAG(10/8)
BsiEI          62  CGGCCG               CG[GA][CT]^CG
BsiHKAI       169  GAGCTC               G[AT]GC[AT]^C
BsmFI          77  GGGAC                GGGAC(10/14)
BsrBI         375  CCGCTC               CCGCTC(-3/-3)
BssSI          13  CACGAG               CACGAG(-5/-1)
BstNI         324  CCTGG                CC^[AT]GG
BstYI         596  AGATCC               [GA]^GATC[CT]
Bsu36I        388  CCTAAGG              CC^T[ACGT]AGG
Cac8I         167  GCGAGC               GC[ACGT]^[ACGT]GC
CelII         377  GCTCAGC              GC^T[ACGT]AGC
Cfr13I         78  GGACC                G^G[ACGT]CC
DraII          77  GGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI           42  GACAGGGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           62  CGGCCG               [CT]^GGCC[GA]
EagI           62  CGGCCG               C^GGCCG
EclXI          62  CGGCCG               C^GGCCG
Eco52I         62  CGGCCG               C^GGCCG
Eco81I        388  CCTAAGG              CC^T[ACGT]AGG
EcoO109I       77  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRII        324  CCTGG                ^CC[AT]GG
EcoT14I       473  CCAAGG               C^C[AT][AT]GG
HaeIII         63  GGCC                 GG^CC
HgaI          127  GACGC                GACGC(5/10)
MamI          315  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          596  AGATCC               [GA]^GATC[CT]
MslI          429  CACCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          324  CCTGG                CC^[AT]GG
NdeI          556  CATATG               CA^TATG
PaeR7I        172  CTCGAG               C^TCGAG
PpuMI          77  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         349  CAGCTG               CAG^CTG
SacI          169  GAGCTC               GAGCT^C
Sau96I         78  GGACC                G^G[ACGT]CC
SmlI          172  CTCGAG               C^T[CT][GA]AG
StyI          473  CCAAGG               C^C[AT][AT]GG
XcmI          414  CCAGTCCATCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          172  CTCGAG               C^TCGAG
XhoII         596  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwI          411  GATCC                GGATC(4/5)
              597  GATCC               
BmyI           70  GTGCCC               G[AGT]GC[ACT]^C
              169  GAGCTC              
BsiYI         398  CCATCAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              569  CCGTTAGAAGG         
BslI          398  CCATCAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              569  CCGTTAGAAGG         
BsmBI          58  GAGACG               CGTCTC(1/5)
              125  GAGACG              
Bsp1286I       70  GTGCCC               G[AGT]GC[ACT]^C
              169  GAGCTC              
DpnII         411  GATC                 ^GATC
              597  GATC                
HapII          74  CCGG                 C^CGG
               82  CCGG                
HpaII          74  CCGG                 C^CGG
               82  CCGG                
MboI          411  GATC                 ^GATC
              597  GATC                
MspI           74  CCGG                 C^CGG
               82  CCGG                
NdeII         411  GATC                 ^GATC
              597  GATC                
PstI          152  CTGCAG               CTGCA^G
              236  CTGCAG              
Sau3AI        411  GATC                 ^GATC
              597  GATC                
SfaNI           7  GATGC                GCATC(5/9)
              315  GATGC               
SfcI          152  CTGCAG               C^T[GA][CT]AG
              236  CTGCAG              
SmaI           73  CCCGGG               CCC^GGG
               81  CCCGGG              
TaqI          173  TCGA                 T^CGA
              607  TCGA                
TfiI          104  GAATC                G^A[AT]TC
              306  GATTC               
TspRI         423  CACTG                CA[GC]TG[ACGT][ACGT]^
              506  CACTG               
TthHB8I       173  TCGA                 T^CGA
              607  TCGA                
XmaCI          73  CCCGGG               C^CCGGG
               81  CCCGGG              
XmaI           73  CCCGGG               C^CCGGG
               81  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AciI        AluI        AvaI        BbvI        BcnI        
BsaJI       BsmAI       BsoBI       BsrI        BssKI       
BstF5I      DdeI        Fnu4HI      FokI        HinfI       
ItaI        MboII       MnlI        MseI        MspA1I      
NciI        NlaIV       PleI        ScrFI       Tru9I       
TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com