Restriction map for pF1KB7569
 (Linear) (Six-base) MAPSORT of: KIBB7569.seq  Check: 7900  from: 1  to: 633



KIBB7569.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AleI CACnn'nnGTG

Cuts at:      0     433     633 
   Size:        433     200
 
AvaI C'yCGr_G

Cuts at:      0      73      81     172     633 
   Size:         73       8      91     461

  Fragments arranged by size: 

                 461      91      73       8
 
BanI G'GyrC_C

Cuts at:      0     427     633 
   Size:        427     206
 
BanII G_rGCy'C

Cuts at:      0     173     633 
   Size:        173     460
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     221     633 
   Size:        221     412
 
BlpI GC'TnA_GC

Cuts at:      0     378     633 
   Size:        378     255
 
Bme1580I G_kGCm'C

Cuts at:      0      74     633 
   Size:         74     559
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      39     633 
   Size:         39     594
 
BsaBI GATnn'nnATC

Cuts at:      0     319     633 
   Size:        319     314
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     633 
   Size:        358     275
 
BsiEI CG_ry'CG

Cuts at:      0      65     633 
   Size:         65     568
 
BsiHKAI G_wGCw'C

Cuts at:      0     173     633 
   Size:        173     460
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      52     119     633 
   Size:         52      67     514

  Fragments arranged by size: 

                 514      67      52
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     173     633 
   Size:         74      99     460

  Fragments arranged by size: 

                 460      99      74
 
BsrBI CCG'CTC

Cuts at:      0     377     633 
   Size:        377     256
 
BssSI C'ACGA_G

Cuts at:      0      13     633 
   Size:         13     620
 
BstYI r'GATC_y

Cuts at:      0     596     633 
   Size:        596      37
 
Bsu36I CC'TnA_GG

Cuts at:      0     389     633 
   Size:        389     244
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      48     633 
   Size:         48     585
 
EaeI y'GGCC_r

Cuts at:      0      62     633 
   Size:         62     571
 
EagI C'GGCC_G

Cuts at:      0      62     633 
   Size:         62     571
 
EcoICRI GAG'CTC

Cuts at:      0     171     633 
   Size:        171     462
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     633 
   Size:         78     555
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      50     633 
   Size:         32      18     583

  Fragments arranged by size: 

                 583      32      18
 
MslI CAynn'nnrTG

Cuts at:      0     433     633 
   Size:        433     200
 
MspA1I CmG'CkG

Cuts at:      0      67     157     351     633 
   Size:         67      90     194     282

  Fragments arranged by size: 

                 282     194      90      67
 
NdeI CA'TA_TG

Cuts at:      0     557     633 
   Size:        557      76
 
PpuMI rG'GwC_Cy

Cuts at:      0      78     633 
   Size:         78     555
 
PstI C_TGCA'G

Cuts at:      0     156     240     633 
   Size:        156      84     393

  Fragments arranged by size: 

                 393     156      84
 
PvuII CAG'CTG

Cuts at:      0     351     633 
   Size:        351     282
 
SacI G_AGCT'C

Cuts at:      0     173     633 
   Size:        173     460
 
SanDI GG'GwC_CC

Cuts at:      0      78     633 
   Size:         78     555
 
SfcI C'TryA_G

Cuts at:      0     152     236     633 
   Size:        152      84     397

  Fragments arranged by size: 

                 397     152      84
 
SmaI CCC'GGG

Cuts at:      0      75      83     633 
   Size:         75       8     550

  Fragments arranged by size: 

                 550      75       8
 
SmlI C'TyrA_G

Cuts at:      0     172     633 
   Size:        172     461
 
StyI C'CwwG_G

Cuts at:      0     473     633 
   Size:        473     160
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     421     633 
   Size:        421     212
 
XhoI C'TCGA_G

Cuts at:      0     172     633 
   Size:        172     461
 
XmaI C'CCGG_G

Cuts at:      0      73      81     633 
   Size:         73       8     552

  Fragments arranged by size: 

                 552      73       8
 

 Enzymes that do cut:

     AleI     AvaI     BanI    BanII     BbsI     BlpI Bme1580I     BplI
    BsaBI    BseRI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BssSI
    BstYI   Bsu36I     DrdI     EaeI     EagI  EcoICRI EcoO109I     MmeI
     MslI   MspA1I     NdeI    PpuMI     PstI    PvuII     SacI    SanDI
     SfcI     SmaI     SmlI     StyI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XmnI     ZraI
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