Restriction map for pF1KB7559
 (Circular) (Six-base) MAPSORT of: pF1KB7559.seq  Check: 33  from: 1  to: 3731



pF1KB7559  3731 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    346     590     346 
   Size:        244    3487
 
AccI GT'mk_AC

Cuts at:    769     769 
   Size:       3731
 
Acc65I G'GTAC_C

Cuts at:    747     747 
   Size:       3731
 
AclI AA'CG_TT

Cuts at:   3571    3571 
   Size:       3731
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1556    1988    2735    3003    1556 
   Size:        432     747     268    2284

  Fragments arranged by size: 

                2284     747     432     268
 
AfeI AGC'GCT

Cuts at:    280     280 
   Size:       3731
 
AflIII A'CryG_T

Cuts at:   1083    2187    3164    1083 
   Size:       1104     977    1650

  Fragments arranged by size: 

                1650    1104     977
 
AgeI A'CCGG_T

Cuts at:   2119    2119 
   Size:       3731
 
AhdI GACnn_n'nnGTC

Cuts at:    240     240 
   Size:       3731
 
AlwNI CAG_nnn'CTG

Cuts at:   1036    2603    1036 
   Size:       1567    2164
 
ApaLI G'TGCA_C

Cuts at:    625    2501     625 
   Size:       1876    1855
 
ApoI r'AATT_y

Cuts at:    735    3084    3340     735 
   Size:       2349     256    1126

  Fragments arranged by size: 

                2349    1126     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3731
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3731
 
AvaI C'yCGr_G

Cuts at:    660     751     660 
   Size:         91    3640
 
BamHI G'GATC_C

Cuts at:    756     756 
   Size:       3731
 
BanI G'GyrC_C

Cuts at:    516     747    1161    1409    1444     516 
   Size:        231     414     248      35    2803

  Fragments arranged by size: 

                2803     414     248     231      35
 
BanII G_rGCy'C

Cuts at:    121     406     745    1775     121 
   Size:        285     339    1030    2077

  Fragments arranged by size: 

                2077    1030     339     285
 
BbeI G_GCGC'C

Cuts at:   1165    1413    1165 
   Size:        248    3483
 
BbsI GAAGACnn'nnnn_

Cuts at:    672     672 
   Size:       3731
 
BciVI GTATCCnnnnn_n'

Cuts at:   1629    2390    3731    1629 
   Size:        761    1341    1629

  Fragments arranged by size: 

                1629    1341     761
 
BclI T'GATC_A

Cuts at:   1251    1251 
   Size:       3731
 
BglII A'GATC_T

Cuts at:   1246    3283    1246 
   Size:       2037    1694
 
BlpI GC'TnA_GC

Cuts at:    405     475     843     405 
   Size:         70     368    3293

  Fragments arranged by size: 

                3293     368      70
 
Bme1580I G_kGCm'C

Cuts at:    295     462     521     629    1356    1449    2505     295 
   Size:        167      59     108     727      93    1056    1521

  Fragments arranged by size: 

                1521    1056     727     167     108      93      59
 
BmrI ACTGGGnnnn_n'

Cuts at:    224     625     943    1024    1359    3503     224 
   Size:        401     318      81     335    2144     452

  Fragments arranged by size: 

                2144     452     401     335     318      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2098    2098 
   Size:       3731
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    474     474 
   Size:       3731
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    481     907    1786    2278    2576     481 
   Size:        426     879     492     298    1636

  Fragments arranged by size: 

                1636     879     492     426     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    663    3435     663 
   Size:       2772     959
 
BsaAI yAC'GTr

Cuts at:    624    1084     624 
   Size:        460    3271
 
BsaBI GATnn'nnATC

Cuts at:      6    1245    3282       6 
   Size:       1239    2037     455

  Fragments arranged by size: 

                2037    1239     455
 
BsaHI Gr'CG_yC

Cuts at:   1162    1410    2112    3608    1162 
   Size:        248     702    1496    1285

  Fragments arranged by size: 

                1496    1285     702     248
 
BsaWI w'CCGG_w

Cuts at:    132     669     920    1441    2119    2393    2540    2951     132 
   Size:        537     251     521     678     274     147     411     912

  Fragments arranged by size: 

                 912     678     537     521     411     274     251     147
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    151     265     268     286     289     151 
   Size:        114       3      18       3    3593

  Fragments arranged by size: 

                3593     114      18       3       3
 
BseYI C'CCAG_C

Cuts at:    340    1156    1914    2491    2987     340 
   Size:        816     758     577     496    1084

  Fragments arranged by size: 

                1084     816     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1273    1319    2527     104 
   Size:       1169      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1169      46
 
BsiHKAI G_wGCw'C

Cuts at:    629     745    1523    1713    2505     629 
   Size:        116     778     190     792    1855

  Fragments arranged by size: 

                1855     792     778     190     116
 
Bsp1286I G_dGCh'C

Cuts at:    121     295     406     462     521     629     745    1356    1449 
   Size:        174     111      56      59     108     116     611      93

Cuts at:   1449    1523    1713    1775    2505     121 
   Size:         74     190      62     730    1347

  Fragments arranged by size: 

                1347     730     611     190     174     116     111     108
                  93      74      62      59      56
 
BspEI T'CCGG_A

Cuts at:    132    2951     132 
   Size:       2819     912
 
BspHI T'CATG_A

Cuts at:   2907    2907 
   Size:       3731
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    346     590     781     995    1297    1678     346 
   Size:        244     191     214     302     381    2399

  Fragments arranged by size: 

                2399     381     302     244     214     191
 
BsrBI CCG'CTC

Cuts at:     50    2023    2077    3561    3726      50 
   Size:       1973      54    1484     165      55

  Fragments arranged by size: 

                1973    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1643    1643 
   Size:       3731
 
BsrFI r'CCGG_y

Cuts at:    298    1729    1910    2119     298 
   Size:       1431     181     209    1910

  Fragments arranged by size: 

                1910    1431     209     181
 
BssSI C'ACGA_G

Cuts at:   2002    2360    2002 
   Size:        358    3373
 
BstAPI GCAn_nnn'nTGC

Cuts at:    594    1108     594 
   Size:        514    3217
 
BstBI TT'CG_AA

Cuts at:   2092    2092 
   Size:       3731
 
BstYI r'GATC_y

Cuts at:      1     756    1223    1246    1581    1827    2839    2925    2937 
   Size:        755     467      23     335     246    1012      86      12

Cuts at:   2937    3283       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     755     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    113     201     113 
   Size:         88    3643
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    558    1681    1862    2025     558 
   Size:       1123     181     163    2264

  Fragments arranged by size: 

                2264    1123     181     163
 
BtsI GCAGTG_nn'

Cuts at:    974     974 
   Size:       3731
 
DraI TTT'AAA

Cuts at:    730     730 
   Size:       3731
 
DraIII CAC_nnn'GTG

Cuts at:    633     633 
   Size:       3731
 
DrdI GACnn_nn'nnGTC

Cuts at:   1437    2295    1437 
   Size:        858    2873
 
EaeI y'GGCC_r

Cuts at:    395     440     646    1316    1490    1881    1908    3253     395 
   Size:         45     206     670     174     391      27    1345     873

  Fragments arranged by size: 

                1345     873     670     391     206     174      45      27
            
 
EagI C'GGCC_G

Cuts at:   1316    1316 
   Size:       3731
 
EarI CTCTTCn'nnn_

Cuts at:   1754    1964    1754 
   Size:        210    3521
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1939    2249    2395    3541    1939 
   Size:        310     146    1146    2129

  Fragments arranged by size: 

                2129    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    743     743 
   Size:       3731
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    474    1556    1988    2735    3003     474 
   Size:       1082     432     747     268    1202

  Fragments arranged by size: 

                1202    1082     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    186     489     186 
   Size:        303    3428
 
EcoO109I rG'GnC_Cy

Cuts at:    258     870     258 
   Size:        612    3119
 
EcoRI G'AATT_C

Cuts at:    735     735 
   Size:       3731
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1750    1750 
   Size:       3731
 
FspI TGC'GCA

Cuts at:   1512    1512 
   Size:       3731
 
HaeII r_GCGC'y

Cuts at:    282    1165    1413    2435    3414     282 
   Size:        883     248    1022     979     599

  Fragments arranged by size: 

                1022     979     883     599     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    226     258    1573    1603    1605    1635     226 
   Size:         32    1315      30       2      30    2322

  Fragments arranged by size: 

                2322    1315      32      30      30       2
 
HincII GTy'rAC

Cuts at:    770     770 
   Size:       3731
 
KasI G'GCGC_C

Cuts at:   1161    1409    1161 
   Size:        248    3483
 
KpnI G_GTAC'C

Cuts at:    751     751 
   Size:       3731
 
MluI A'CGCG_T

Cuts at:   3164    3164 
   Size:       3731
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2402    2586    2402 
   Size:        184    3547
 
MscI TGG'CCA

Cuts at:    648    1492     648 
   Size:        844    2887
 
MslI CAynn'nnrTG

Cuts at:   1720    1847    1720 
   Size:        127    3604
 
MspA1I CmG'CkG

Cuts at:    162     203     432     591     724     842    1156    1516    2529 
   Size:         41     229     159     133     118     314     360    1013

Cuts at:   2529    2774     162 
   Size:        245    1119

  Fragments arranged by size: 

                1119    1013     360     314     245     229     159     133
                 118      41
 
NaeI GCC'GGC

Cuts at:    300    1912     300 
   Size:       1612    2119
 
NarI GG'CG_CC

Cuts at:   1162    1410    1162 
   Size:        248    3483
 
NgoMIV G'CCGG_C

Cuts at:    298    1910     298 
   Size:       1612    2119
 
NspI r_CATG'y

Cuts at:    784    1284    2191     784 
   Size:        500     907    2324

  Fragments arranged by size: 

                2324     907     500
 
PasI CC'CwG_GG

Cuts at:    512     512 
   Size:       3731
 
PciI A'CATG_T

Cuts at:   2187    2187 
   Size:       3731
 
PmeI GTTT'AAAC

Cuts at:    730     730 
   Size:       3731
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2870    2902    2870 
   Size:         32    3699
 
PpuMI rG'GwC_Cy

Cuts at:    258     258 
   Size:       3731
 
PstI C_TGCA'G

Cuts at:    161     778    1463     161 
   Size:        617     685    2429

  Fragments arranged by size: 

                2429     685     617
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3731
 
PvuII CAG'CTG

Cuts at:    591    1156    1516     591 
   Size:        565     360    2806

  Fragments arranged by size: 

                2806     565     360
 
RsrII CG'GwC_CG

Cuts at:   1926    1926 
   Size:       3731
 
SacI G_AGCT'C

Cuts at:    745     745 
   Size:       3731
 
SacII CC_GC'GG

Cuts at:    204     204 
   Size:       3731
 
SalI G'TCGA_C

Cuts at:    768     768 
   Size:       3731
 
SapI GCTCTTCn'nnn_

Cuts at:   1754    1964    1754 
   Size:        210    3521
 
SbfI CC_TGCA'GG

Cuts at:    778     778 
   Size:       3731
 
ScaI AGT'ACT

Cuts at:   3248    3248 
   Size:       3731
 
SfcI C'TryA_G

Cuts at:     33     157     774    1459    2452    2643      33 
   Size:        124     617     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     617     191     124
 
SfoI GGC'GCC

Cuts at:   1163    1411    1163 
   Size:        248    3483
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3731
 
SmaI CCC'GGG

Cuts at:    753     753 
   Size:       3731
 
SmlI C'TyrA_G

Cuts at:    496     886    1801    2293    2555     496 
   Size:        390     915     492     262    1672

  Fragments arranged by size: 

                1672     915     492     390     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3731
 
SphI G_CATG'C

Cuts at:    784    1284     784 
   Size:        500    3231
 
StyI C'CwwG_G

Cuts at:    398     865    1219     398 
   Size:        467     354    2910

  Fragments arranged by size: 

                2910     467     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    504     644    1314    1447    1906    2115     504 
   Size:        140     670     133     459     209    2120

  Fragments arranged by size: 

                2120     670     459     209     140     133
 
TatI w'GTAC_w

Cuts at:   3246    3246 
   Size:       3731
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3248    3280    3248 
   Size:         32    3699
 
Tth111I GACn'n_nGTC

Cuts at:   1528    1528 
   Size:       3731
 
XbaI T'CTAG_A

Cuts at:    762     762 
   Size:       3731
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1226    1226 
   Size:       3731
 
XmaI C'CCGG_G

Cuts at:    751     751 
   Size:       3731
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PasI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AleI     AlfI     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI   Bpu10I
    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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