Restriction map for pF1KB7557
 (Circular) (Six-base) MAPSORT of: pF1KB7557.seq  Check: 3906  from: 1  to: 3719



pF1KB7557  3719 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    667     757     667 
   Size:         90    3629
 
Acc65I G'GTAC_C

Cuts at:    735     735 
   Size:       3719
 
AclI AA'CG_TT

Cuts at:    253    3559     253 
   Size:       3306     413
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     521    1544    1976    2723    2991     199 
   Size:        322    1023     432     747     268     927

  Fragments arranged by size: 

                1023     927     747     432     322     268
 
AflIII A'CryG_T

Cuts at:   1071    2175    3152    1071 
   Size:       1104     977    1638

  Fragments arranged by size: 

                1638    1104     977
 
AgeI A'CCGG_T

Cuts at:   2107    2107 
   Size:       3719
 
AleI CACnn'nnGTG

Cuts at:    157     157 
   Size:       3719
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    530     530 
   Size:       3719
 
AlwNI CAG_nnn'CTG

Cuts at:   1024    2591    1024 
   Size:       1567    2152
 
ApaI G_GGCC'C

Cuts at:    595     595 
   Size:       3719
 
ApaLI G'TGCA_C

Cuts at:   2489    2489 
   Size:       3719
 
ApoI r'AATT_y

Cuts at:    454     723    3072    3328     454 
   Size:        269    2349     256     845

  Fragments arranged by size: 

                2349     845     269     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3719
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3719
 
AvaI C'yCGr_G

Cuts at:    739     739 
   Size:       3719
 
BamHI G'GATC_C

Cuts at:    744     744 
   Size:       3719
 
BanI G'GyrC_C

Cuts at:    151     735    1149    1397    1432     151 
   Size:        584     414     248      35    2438

  Fragments arranged by size: 

                2438     584     414     248      35
 
BanII G_rGCy'C

Cuts at:    595     733    1763     595 
   Size:        138    1030    2551

  Fragments arranged by size: 

                2551    1030     138
 
BbeI G_GCGC'C

Cuts at:   1153    1401    1153 
   Size:        248    3471
 
BbsI GAAGACnn'nnnn_

Cuts at:    400     400 
   Size:       3719
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    237     271     237 
   Size:         34    3685
 
BciVI GTATCCnnnnn_n'

Cuts at:   1617    2378    3719    1617 
   Size:        761    1341    1617

  Fragments arranged by size: 

                1617    1341     761
 
BclI T'GATC_A

Cuts at:   1239    1239 
   Size:       3719
 
BglII A'GATC_T

Cuts at:    220    1234    3271     220 
   Size:       1014    2037     668

  Fragments arranged by size: 

                2037    1014     668
 
BlpI GC'TnA_GC

Cuts at:    831     831 
   Size:       3719
 
Bme1580I G_kGCm'C

Cuts at:    595    1344    1437    2493     595 
   Size:        749      93    1056    1821

  Fragments arranged by size: 

                1821    1056     749      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    931    1012    1347    3491     931 
   Size:         81     335    2144    1159

  Fragments arranged by size: 

                2144    1159     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    559    2086     559 
   Size:       1527    2192
 
Bpu10I CC'TnA_GC

Cuts at:    167     167 
   Size:       3719
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    209     549     895    1774    2266    2564     209 
   Size:        340     346     879     492     298    1364

  Fragments arranged by size: 

                1364     879     492     346     340     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3423    3423 
   Size:       3719
 
BsaAI yAC'GTr

Cuts at:   1072    1072 
   Size:       3719
 
BsaBI GATnn'nnATC

Cuts at:      6     437    1233    3270       6 
   Size:        431     796    2037     455

  Fragments arranged by size: 

                2037     796     455     431
 
BsaHI Gr'CG_yC

Cuts at:   1150    1398    2100    3596    1150 
   Size:        248     702    1496    1273

  Fragments arranged by size: 

                1496    1273     702     248
 
BsaWI w'CCGG_w

Cuts at:    908    1429    2107    2381    2528    2939     908 
   Size:        521     678     274     147     411    1688

  Fragments arranged by size: 

                1688     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    574     574 
   Size:       3719
 
BseYI C'CCAG_C

Cuts at:    183     588    1144    1902    2479    2975     183 
   Size:        405     556     758     577     496     927

  Fragments arranged by size: 

                 927     758     577     556     496     405
 
BsiEI CG_ry'CG

Cuts at:    104    1261    1307    2515     104 
   Size:       1157      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1157      46
 
BsiHKAI G_wGCw'C

Cuts at:    509     733    1511    1701    2493     509 
   Size:        224     778     190     792    1735

  Fragments arranged by size: 

                1735     792     778     224     190
 
BsmI GAATG_Cn'

Cuts at:    659     659 
   Size:       3719
 
Bsp1286I G_dGCh'C

Cuts at:    509     595     733    1344    1437    1511    1701    1763    2493 
   Size:         86     138     611      93      74     190      62     730

Cuts at:   2493     509 
   Size:       1735

  Fragments arranged by size: 

                1735     730     611     190     138      93      86      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2939    2939 
   Size:       3719
 
BspHI T'CATG_A

Cuts at:    545    2895     545 
   Size:       2350    1369
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    503     769     983    1285    1666     503 
   Size:        266     214     302     381    2556

  Fragments arranged by size: 

                2556     381     302     266     214
 
BsrBI CCG'CTC

Cuts at:     50    2011    2065    3549    3714      50 
   Size:       1961      54    1484     165      55

  Fragments arranged by size: 

                1961    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1631    1631 
   Size:       3719
 
BsrFI r'CCGG_y

Cuts at:   1717    1898    2107    1717 
   Size:        181     209    3329

  Fragments arranged by size: 

                3329     209     181
 
BssSI C'ACGA_G

Cuts at:   1990    2348    1990 
   Size:        358    3361
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1096    1096 
   Size:       3719
 
BstBI TT'CG_AA

Cuts at:   2080    2080 
   Size:       3719
 
BstYI r'GATC_y

Cuts at:      1     220     744    1211    1234    1569    1815    2827    2913 
   Size:        219     524     467      23     335     246    1012      86

Cuts at:   2913    2925    3271       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     524     467     449     346     335     246     219
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     625     107 
   Size:        518    3201
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1669    1850    2013    1669 
   Size:        181     163    3375

  Fragments arranged by size: 

                3375     181     163
 
BtsI GCAGTG_nn'

Cuts at:    962     962 
   Size:       3719
 
DraI TTT'AAA

Cuts at:    718     718 
   Size:       3719
 
DrdI GACnn_nn'nnGTC

Cuts at:   1425    2283    1425 
   Size:        858    2861
 
EaeI y'GGCC_r

Cuts at:   1304    1478    1869    1896    3241    1304 
   Size:        174     391      27    1345    1782

  Fragments arranged by size: 

                1782    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1304    1304 
   Size:       3719
 
EarI CTCTTCn'nnn_

Cuts at:    497    1742    1952     497 
   Size:       1245     210    2264

  Fragments arranged by size: 

                2264    1245     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1927    2237    2383    3529    1927 
   Size:        310     146    1146    2117

  Fragments arranged by size: 

                2117    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    731     731 
   Size:       3719
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     521    1544    1976    2723    2991     199 
   Size:        322    1023     432     747     268     927

  Fragments arranged by size: 

                1023     927     747     432     322     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    556     556 
   Size:       3719
 
EcoO109I rG'GnC_Cy

Cuts at:    343     592     858     343 
   Size:        249     266    3204

  Fragments arranged by size: 

                3204     266     249
 
EcoRI G'AATT_C

Cuts at:    723     723 
   Size:       3719
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1738    1738 
   Size:       3719
 
FspI TGC'GCA

Cuts at:    518    1500     518 
   Size:        982    2737
 
HaeII r_GCGC'y

Cuts at:   1153    1401    2423    3402    1153 
   Size:        248    1022     979    1470

  Fragments arranged by size: 

                1470    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    185     205     217     237     603     635    1561    1591    1593 
   Size:         20      12      20     366      32     926      30       2

Cuts at:   1593    1623     185 
   Size:         30    2281

  Fragments arranged by size: 

                2281     926     366      32      30      30      20      20
                  12       2
 
HincII GTy'rAC

Cuts at:    309     668     758     309 
   Size:        359      90    3270

  Fragments arranged by size: 

                3270     359      90
 
HindIII A'AGCT_T

Cuts at:    366     366 
   Size:       3719
 
KasI G'GCGC_C

Cuts at:   1149    1397    1149 
   Size:        248    3471
 
KpnI G_GTAC'C

Cuts at:    739     739 
   Size:       3719
 
MluI A'CGCG_T

Cuts at:   3152    3152 
   Size:       3719
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2390    2574    2390 
   Size:        184    3535
 
MscI TGG'CCA

Cuts at:   1480    1480 
   Size:       3719
 
MslI CAynn'nnrTG

Cuts at:    157    1708    1835     157 
   Size:       1551     127    2041

  Fragments arranged by size: 

                2041    1551     127
 
MspA1I CmG'CkG

Cuts at:    588     830    1144    1504    2517    2762     588 
   Size:        242     314     360    1013     245    1545

  Fragments arranged by size: 

                1545    1013     360     314     245     242
 
NaeI GCC'GGC

Cuts at:   1900    1900 
   Size:       3719
 
NarI GG'CG_CC

Cuts at:   1150    1398    1150 
   Size:        248    3471
 
NcoI C'CATG_G

Cuts at:    107     625     107 
   Size:        518    3201
 
NgoMIV G'CCGG_C

Cuts at:   1898    1898 
   Size:       3719
 
NspI r_CATG'y

Cuts at:    772    1272    2179     772 
   Size:        500     907    2312

  Fragments arranged by size: 

                2312     907     500
 
PciI A'CATG_T

Cuts at:   2175    2175 
   Size:       3719
 
PflMI CCAn_nnn'nTGG

Cuts at:    244     244 
   Size:       3719
 
PmeI GTTT'AAAC

Cuts at:    718     718 
   Size:       3719
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2858    2890    2858 
   Size:         32    3687
 
PpuMI rG'GwC_Cy

Cuts at:    343     343 
   Size:       3719
 
PspOMI G'GGCC_C

Cuts at:    591     591 
   Size:       3719
 
PstI C_TGCA'G

Cuts at:    766    1451     766 
   Size:        685    3034
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3719
 
PvuII CAG'CTG

Cuts at:    588    1144    1504     588 
   Size:        556     360    2803

  Fragments arranged by size: 

                2803     556     360
 
RsrII CG'GwC_CG

Cuts at:   1914    1914 
   Size:       3719
 
SacI G_AGCT'C

Cuts at:    733     733 
   Size:       3719
 
SalI G'TCGA_C

Cuts at:    666     756     666 
   Size:         90    3629
 
SanDI GG'GwC_CC

Cuts at:    343     343 
   Size:       3719
 
SapI GCTCTTCn'nnn_

Cuts at:    497    1742    1952     497 
   Size:       1245     210    2264

  Fragments arranged by size: 

                2264    1245     210
 
SbfI CC_TGCA'GG

Cuts at:    766     766 
   Size:       3719
 
ScaI AGT'ACT

Cuts at:   3236    3236 
   Size:       3719
 
SfcI C'TryA_G

Cuts at:     33     317     762    1447    2440    2631      33 
   Size:        284     445     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     445     284     191
 
SfoI GGC'GCC

Cuts at:   1151    1399    1151 
   Size:        248    3471
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3719
 
SmaI CCC'GGG

Cuts at:    741     741 
   Size:       3719
 
SmlI C'TyrA_G

Cuts at:    224     528     874    1789    2281    2543     224 
   Size:        304     346     915     492     262    1400

  Fragments arranged by size: 

                1400     915     492     346     304     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3719
 
SphI G_CATG'C

Cuts at:    772    1272     772 
   Size:        500    3219
 
StyI C'CwwG_G

Cuts at:    107     338     625     853    1207     107 
   Size:        231     287     228     354    2619

  Fragments arranged by size: 

                2619     354     287     231     228
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1302    1435    1894    2103    1302 
   Size:        133     459     209    2918

  Fragments arranged by size: 

                2918     459     209     133
 
TatI w'GTAC_w

Cuts at:   3234    3234 
   Size:       3719
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3236    3268    3236 
   Size:         32    3687
 
Tth111I GACn'n_nGTC

Cuts at:   1516    1516 
   Size:       3719
 
XbaI T'CTAG_A

Cuts at:    474     750     474 
   Size:        276    3443
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1214    1214 
   Size:       3719
 
XmaI C'CCGG_G

Cuts at:    739     739 
   Size:       3719
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     AscI    AvrII
     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI     BpmI    BsaXI
     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmlI    PshAI     PsiI    PspXI     PsrI    SacII    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com