Restriction map for pF1KB7556
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        DsaI        
Eam1105I    Eco47III    EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AocI          634  CCTGAGG              CC^T[ACGT]AGG
AspHI          30  GAGCTC               G[AT]GC[AT]^C
AvaI           27  CCCGAG               C^[CT]CG[GA]G
BbsI          700  GTCTTC               GAAGAC(2/6)
BbvCI         734  CCTCAGC              CCTCAGC(-5/-2)
BcgI          505  CGACCCATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          481  CTAG                 C^TAG
BpmI          266  CTCCAG               CTGGAG(16/14)
BpuAI         700  GTCTTC               GAAGAC(2/6)
BsaI          767  GAGACC               GGTCTC(1/5)
BsaWI         371  ACCGGA               [AT]^CCGG[AT]
BsiEI          74  CGGCCG               CG[GA][CT]^CG
BsiHKAI        30  GAGCTC               G[AT]GC[AT]^C
BsmFI         542  GGGAC                GGGAC(10/14)
BsoBI          27  CCCGAG               C^[CT]CG[GA]G
BspHI         659  TCATGA               T^CATGA
BsrBI         337  GAGCGG               CCGCTC(-3/-3)
Bsu36I        634  CCTGAGG              CC^T[ACGT]AGG
DpnII         297  GATC                 ^GATC
DraIII        106  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           74  CGGCCG               C^GGCCG
EarI          310  GAAGAG               CTCTTC(1/4)
EclXI          74  CGGCCG               C^GGCCG
Eco52I         74  CGGCCG               C^GGCCG
Eco81I        634  CCTGAGG              CC^T[ACGT]AGG
HinfI         242  GAGTC                G^A[ACGT]TC
Ksp632I       310  GAAGAG               CTCTTC(1/4)
MaeI          481  CTAG                 C^TAG
MaeII         276  ACGT                 A^CGT
MaeIII        188  GTTAC                ^GT[ACGT]AC
MboI          297  GATC                 ^GATC
MslI          654  CACACTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        762  CAGCGG               C[AC]G^C[GT]G
NdeII         297  GATC                 ^GATC
PleI          242  GAGTC                GAGTC(4/5)
RcaI          659  TCATGA               T^CATGA
SacI           30  GAGCTC               GAGCT^C
Sau3AI        297  GATC                 ^GATC
SbfI          396  CCTGCAGG             CCTGCA^GG
SexAI         107  ACCTGGT              A^CC[AT]GGT
SfaNI         119  GCATC                GCATC(5/9)
Sse8387I      396  CCTGCAGG             CCTGCA^GG
TaqI          279  TCGA                 T^CGA
TthHB8I       279  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccII          63  CGCG                 CG^CG
              334  CGCG                
AlwNI         142  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              617  CAGGGCCTG           
ApaI          178  GGGCCC               GGGCC^C
              251  GGGCCC              
BanI          111  GGTGCC               G^G[CT][GA]CC
              562  GGCACC              
BsgI          643  CTGCAC               GTGCAG(16/14)
              363  GTGCAG              
BsrI          417  ACTGG                ACTGG(1/-1)
              557  CCAGT               
BstUI          63  CGCG                 CG^CG
              334  CGCG                
EcoT14I       533  CCAAGG               C^C[AT][AT]GG
              786  CCTTGG              
FauI           61  CCCGC                CCCGC(4/6)
               47  GCGGG               
HaeII         317  AGCGCC               [GA]GCGC^[CT]
              476  AGCGCC              
HphI          686  TCACC                GGTGA(8/7)
              704  TCACC               
MvnI           63  CGCG                 CG^CG
              334  CGCG                
NlaIII        129  CATG                 CATG^
              660  CATG                
StuI          196  AGGCCT               AGG^CCT
              283  AGGCCT              
StyI          533  CCAAGG               C^C[AT][AT]GG
              786  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AluI        BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       EaeI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NlaIV       PstI        
RsaI        Sau96I      ScrFI       SfcI        TseI        
TspRI       
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