Restriction map for pF1KB7556
 (Linear) (Six-base) MAPSORT of: KIBB7556.seq  Check: 6991  from: 1  to: 813



KIBB7556.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     147     622     813 
   Size:        147     475     191

  Fragments arranged by size: 

                 475     191     147
 
ApaI G_GGCC'C

Cuts at:      0     182     255     813 
   Size:        182      73     558

  Fragments arranged by size: 

                 558     182      73
 
AvaI C'yCGr_G

Cuts at:      0      27     813 
   Size:         27     786
 
BanI G'GyrC_C

Cuts at:      0     111     562     813 
   Size:        111     451     251

  Fragments arranged by size: 

                 451     251     111
 
BanII G_rGCy'C

Cuts at:      0      34     182     255     813 
   Size:         34     148      73     558

  Fragments arranged by size: 

                 558     148      73      34
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     693     813 
   Size:        693     120
 
BbvCI CC'TCA_GC

Cuts at:      0     735     813 
   Size:        735      78
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     494     528     813 
   Size:        494      34     285

  Fragments arranged by size: 

                 494     285      34
 
Bme1580I G_kGCm'C

Cuts at:      0     182     255     813 
   Size:        182      73     558

  Fragments arranged by size: 

                 558     182      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     813 
   Size:        251     562
 
Bpu10I CC'TnA_GC

Cuts at:      0     722     735     813 
   Size:        722      13      78

  Fragments arranged by size: 

                 722      78      13
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     761     813 
   Size:        761      52
 
BsaWI w'CCGG_w

Cuts at:      0     371     813 
   Size:        371     442
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     136     166     813 
   Size:        136      30     647

  Fragments arranged by size: 

                 647     136      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328     469     793     813 
   Size:        328     141     324      20

  Fragments arranged by size: 

                 328     324     141      20
 
BseYI C'CCAG_C

Cuts at:      0      92     181     691     712     813 
   Size:         92      89     510      21     101

  Fragments arranged by size: 

                 510     101      92      89      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     384     628     813 
   Size:        384     244     185

  Fragments arranged by size: 

                 384     244     185
 
BsiEI CG_ry'CG

Cuts at:      0      77     813 
   Size:         77     736
 
BsiHKAI G_wGCw'C

Cuts at:      0      34     813 
   Size:         34     779
 
Bsp1286I G_dGCh'C

Cuts at:      0      34     182     255     813 
   Size:         34     148      73     558

  Fragments arranged by size: 

                 558     148      73      34
 
BspHI T'CATG_A

Cuts at:      0     659     813 
   Size:        659     154
 
BsrBI CCG'CTC

Cuts at:      0     339     813 
   Size:        339     474
 
Bsu36I CC'TnA_GG

Cuts at:      0     635     813 
   Size:        635     178
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     727     813 
   Size:        727      86
 
BtsI GCAGTG_nn'

Cuts at:      0     157     640     813 
   Size:        157     483     173

  Fragments arranged by size: 

                 483     173     157
 
DraIII CAC_nnn'GTG

Cuts at:      0     111     813 
   Size:        111     702
 
EaeI y'GGCC_r

Cuts at:      0      74     235     356     813 
   Size:         74     161     121     457

  Fragments arranged by size: 

                 457     161     121      74
 
EagI C'GGCC_G

Cuts at:      0      74     813 
   Size:         74     739
 
EarI CTCTTCn'nnn_

Cuts at:      0     305     813 
   Size:        305     508
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     418     813 
   Size:        418     395
 
EcoICRI GAG'CTC

Cuts at:      0      32     813 
   Size:         32     781
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     813 
   Size:        251     562
 
EcoO109I rG'GnC_Cy

Cuts at:      0     178     252     619     813 
   Size:        178      74     367     194

  Fragments arranged by size: 

                 367     194     178      74
 
HaeII r_GCGC'y

Cuts at:      0     321     480     813 
   Size:        321     159     333

  Fragments arranged by size: 

                 333     321     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     123     155     195     227     415     447     760     792 
   Size:        123      32      40      32     188      32     313      32

Cuts at:    792     813 
   Size:         21

  Fragments arranged by size: 

                 313     188     123      40      32      32      32      32
                  21
 
MslI CAynn'nnrTG

Cuts at:      0     658     813 
   Size:        658     155
 
MspA1I CmG'CkG

Cuts at:      0     764     813 
   Size:        764      49
 
PasI CC'CwG_GG

Cuts at:      0     255     616     813 
   Size:        255     361     197

  Fragments arranged by size: 

                 361     255     197
 
PfoI T'CCnGG_A

Cuts at:      0      34     521     813 
   Size:         34     487     292

  Fragments arranged by size: 

                 487     292      34
 
PspOMI G'GGCC_C

Cuts at:      0     178     251     813 
   Size:        178      73     562

  Fragments arranged by size: 

                 562     178      73
 
PstI C_TGCA'G

Cuts at:      0     152     401     443     464     813 
   Size:        152     249      42      21     349

  Fragments arranged by size: 

                 349     249     152      42      21
 
SacI G_AGCT'C

Cuts at:      0      34     813 
   Size:         34     779
 
SbfI CC_TGCA'GG

Cuts at:      0     401     813 
   Size:        401     412
 
SexAI A'CCwGG_T

Cuts at:      0     107     813 
   Size:        107     706
 
SfcI C'TryA_G

Cuts at:      0     148     397     439     460     813 
   Size:        148     249      42      21     353

  Fragments arranged by size: 

                 353     249     148      42      21
 
StuI AGG'CCT

Cuts at:      0     198     285     813 
   Size:        198      87     528

  Fragments arranged by size: 

                 528     198      87
 
StyI C'CwwG_G

Cuts at:      0     533     786     813 
   Size:        533     253      27

  Fragments arranged by size: 

                 533     253      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     403     813 
   Size:        403     410
 
TatI w'GTAC_w

Cuts at:      0     215     813 
   Size:        215     598
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     136     168     813 
   Size:        136      32     645

  Fragments arranged by size: 

                 645     136      32
 

 Enzymes that do cut:

    AlwNI     ApaI     AvaI     BanI    BanII     BbsI    BbvCI     BcgI
 Bme1580I     BpmI   Bpu10I     BsaI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrBI   Bsu36I    BtgZI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    HaeII    Hin4I     MslI   MspA1I     PasI     PfoI   PspOMI
     PstI     SacI     SbfI    SexAI     SfcI     StuI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI     ClaI    CspCI
     DraI     DrdI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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