Restriction map for pF1KB7549
 (Circular) (Six-base) MAPSORT of: pF1KB7549.seq  Check: 5620  from: 1  to: 3692



pF1KB7549  3692 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    730     730 
   Size:       3692
 
Acc65I G'GTAC_C

Cuts at:    708     708 
   Size:       3692
 
AclI AA'CG_TT

Cuts at:   3532    3532 
   Size:       3692
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    376    1517    1949    2696    2964     376 
   Size:       1141     432     747     268    1104

  Fragments arranged by size: 

                1141    1104     747     432     268
 
AflIII A'CryG_T

Cuts at:    119    1044    2148    3125     119 
   Size:        925    1104     977     686

  Fragments arranged by size: 

                1104     977     925     686
 
AgeI A'CCGG_T

Cuts at:   2080    2080 
   Size:       3692
 
AhdI GACnn_n'nnGTC

Cuts at:    234     234 
   Size:       3692
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    187     593     187 
   Size:        406    3286
 
AlwNI CAG_nnn'CTG

Cuts at:    240     997    2564     240 
   Size:        757    1567    1368

  Fragments arranged by size: 

                1567    1368     757
 
ApaLI G'TGCA_C

Cuts at:   2462    2462 
   Size:       3692
 
ApoI r'AATT_y

Cuts at:    696    3045    3301     696 
   Size:       2349     256    1087

  Fragments arranged by size: 

                2349    1087     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3692
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3692
 
AvaI C'yCGr_G

Cuts at:    443     712     443 
   Size:        269    3423
 
BamHI G'GATC_C

Cuts at:    717     717 
   Size:       3692
 
BanI G'GyrC_C

Cuts at:    541     708    1122    1370    1405     541 
   Size:        167     414     248      35    2828

  Fragments arranged by size: 

                2828     414     248     167      35
 
BanII G_rGCy'C

Cuts at:    706    1736     706 
   Size:       1030    2662
 
BbeI G_GCGC'C

Cuts at:   1126    1374    1126 
   Size:        248    3444
 
BbsI GAAGACnn'nnnn_

Cuts at:    313     313 
   Size:       3692
 
BciVI GTATCCnnnnn_n'

Cuts at:   1590    2351    3692    1590 
   Size:        761    1341    1590

  Fragments arranged by size: 

                1590    1341     761
 
BclI T'GATC_A

Cuts at:   1212    1212 
   Size:       3692
 
BglI GCCn_nnn'nGGC

Cuts at:    477     477 
   Size:       3692
 
BglII A'GATC_T

Cuts at:   1207    3244    1207 
   Size:       2037    1655
 
BlpI GC'TnA_GC

Cuts at:    804     804 
   Size:       3692
 
Bme1580I G_kGCm'C

Cuts at:   1317    1410    2466    1317 
   Size:         93    1056    2543

  Fragments arranged by size: 

                2543    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    658     904     985    1320    3464     658 
   Size:        246      81     335    2144     886

  Fragments arranged by size: 

                2144     886     335     246      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2059    2059 
   Size:       3692
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    868    1747    2239    2537     868 
   Size:        879     492     298    2023

  Fragments arranged by size: 

                2023     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    550    3396     550 
   Size:       2846     846
 
BsaAI yAC'GTr

Cuts at:   1045    1045 
   Size:       3692
 
BsaBI GATnn'nnATC

Cuts at:      6    1206    3243       6 
   Size:       1200    2037     455

  Fragments arranged by size: 

                2037    1200     455
 
BsaHI Gr'CG_yC

Cuts at:   1123    1371    2073    3569    1123 
   Size:        248     702    1496    1246

  Fragments arranged by size: 

                1496    1246     702     248
 
BsaWI w'CCGG_w

Cuts at:    881    1402    2080    2354    2501    2912     881 
   Size:        521     678     274     147     411    1661

  Fragments arranged by size: 

                1661     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    644     674     644 
   Size:         30    3662
 
BseYI C'CCAG_C

Cuts at:    312    1117    1875    2452    2948     312 
   Size:        805     758     577     496    1056

  Fragments arranged by size: 

                1056     805     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    400     447     589     400 
   Size:         47     142    3503

  Fragments arranged by size: 

                3503     142      47
 
BsiEI CG_ry'CG

Cuts at:    104    1234    1280    2488     104 
   Size:       1130      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1130      46
 
BsiHKAI G_wGCw'C

Cuts at:    439     706    1484    1674    2466     439 
   Size:        267     778     190     792    1665

  Fragments arranged by size: 

                1665     792     778     267     190
 
Bsp1286I G_dGCh'C

Cuts at:    439     706    1317    1410    1484    1674    1736    2466     439 
   Size:        267     611      93      74     190      62     730    1665

  Fragments arranged by size: 

                1665     730     611     267     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2912    2912 
   Size:       3692
 
BspHI T'CATG_A

Cuts at:    338    2868     338 
   Size:       2530    1162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    372     742     956    1258    1639     372 
   Size:        370     214     302     381    2425

  Fragments arranged by size: 

                2425     381     370     302     214
 
BsrBI CCG'CTC

Cuts at:     50     131    1984    2038    3522    3687      50 
   Size:         81    1853      54    1484     165      55

  Fragments arranged by size: 

                1853    1484     165      81      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1604    1604 
   Size:       3692
 
BsrFI r'CCGG_y

Cuts at:    639    1690    1871    2080     639 
   Size:       1051     181     209    2251

  Fragments arranged by size: 

                2251    1051     209     181
 
BssSI C'ACGA_G

Cuts at:   1963    2321    1963 
   Size:        358    3334
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1069    1069 
   Size:       3692
 
BstBI TT'CG_AA

Cuts at:    374    2053     374 
   Size:       1679    2013
 
BstXI CCAn_nnnn'nTGG

Cuts at:    480     480 
   Size:       3692
 
BstYI r'GATC_y

Cuts at:      1     572     717    1184    1207    1542    1788    2800    2886 
   Size:        571     145     467      23     335     246    1012      86

Cuts at:   2886    2898    3244       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     571     467     449     346     335     246     145
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1642    1823    1986    1642 
   Size:        181     163    3348

  Fragments arranged by size: 

                3348     181     163
 
BtsI GCAGTG_nn'

Cuts at:    935     935 
   Size:       3692
 
DraI TTT'AAA

Cuts at:    691     691 
   Size:       3692
 
DrdI GACnn_nn'nnGTC

Cuts at:   1398    2256    1398 
   Size:        858    2834
 
EaeI y'GGCC_r

Cuts at:    201    1277    1451    1842    1869    3214     201 
   Size:       1076     174     391      27    1345     679

  Fragments arranged by size: 

                1345    1076     679     391     174      27
 
EagI C'GGCC_G

Cuts at:   1277    1277 
   Size:       3692
 
EarI CTCTTCn'nnn_

Cuts at:   1715    1925    1715 
   Size:        210    3482
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1900    2210    2356    3502    1900 
   Size:        310     146    1146    2090

  Fragments arranged by size: 

                2090    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    704     704 
   Size:       3692
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    376    1517    1949    2696    2964     376 
   Size:       1141     432     747     268    1104

  Fragments arranged by size: 

                1141    1104     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    831     831 
   Size:       3692
 
EcoRI G'AATT_C

Cuts at:    696     696 
   Size:       3692
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1711    1711 
   Size:       3692
 
FspI TGC'GCA

Cuts at:   1473    1473 
   Size:       3692
 
HaeII r_GCGC'y

Cuts at:   1126    1374    2396    3375    1126 
   Size:        248    1022     979    1443

  Fragments arranged by size: 

                1443    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    220     252     557     589    1534    1564    1566    1596     220 
   Size:         32     305      32     945      30       2      30    2316

  Fragments arranged by size: 

                2316     945     305      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    731     731 
   Size:       3692
 
KasI G'GCGC_C

Cuts at:   1122    1370    1122 
   Size:        248    3444
 
KpnI G_GTAC'C

Cuts at:    712     712 
   Size:       3692
 
MluI A'CGCG_T

Cuts at:   3125    3125 
   Size:       3692
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    618    2363    2547     618 
   Size:       1745     184    1763

  Fragments arranged by size: 

                1763    1745     184
 
MscI TGG'CCA

Cuts at:    203    1453     203 
   Size:       1250    2442
 
MslI CAynn'nnrTG

Cuts at:    472    1681    1808     472 
   Size:       1209     127    2356

  Fragments arranged by size: 

                2356    1209     127
 
MspA1I CmG'CkG

Cuts at:    803    1117    1477    2490    2735     803 
   Size:        314     360    1013     245    1760

  Fragments arranged by size: 

                1760    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    641    1873     641 
   Size:       1232    2460
 
NarI GG'CG_CC

Cuts at:   1123    1371    1123 
   Size:        248    3444
 
NgoMIV G'CCGG_C

Cuts at:    639    1871     639 
   Size:       1232    2460
 
NspI r_CATG'y

Cuts at:    290     745    1245    2152     290 
   Size:        455     500     907    1830

  Fragments arranged by size: 

                1830     907     500     455
 
PciI A'CATG_T

Cuts at:   2148    2148 
   Size:       3692
 
PflMI CCAn_nnn'nTGG

Cuts at:    240     240 
   Size:       3692
 
PmeI GTTT'AAAC

Cuts at:    691     691 
   Size:       3692
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2831    2863    2831 
   Size:         32    3660
 
PstI C_TGCA'G

Cuts at:    297     739    1424     297 
   Size:        442     685    2565

  Fragments arranged by size: 

                2565     685     442
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3692
 
PvuII CAG'CTG

Cuts at:   1117    1477    1117 
   Size:        360    3332
 
RsrII CG'GwC_CG

Cuts at:   1887    1887 
   Size:       3692
 
SacI G_AGCT'C

Cuts at:    706     706 
   Size:       3692
 
SalI G'TCGA_C

Cuts at:    729     729 
   Size:       3692
 
SapI GCTCTTCn'nnn_

Cuts at:   1715    1925    1715 
   Size:        210    3482
 
SbfI CC_TGCA'GG

Cuts at:    739     739 
   Size:       3692
 
ScaI AGT'ACT

Cuts at:   3209    3209 
   Size:       3692
 
SfcI C'TryA_G

Cuts at:     33     150     293     735    1420    2413    2604      33 
   Size:        117     143     442     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     442     191     143     117
 
SfoI GGC'GCC

Cuts at:   1124    1372    1124 
   Size:        248    3444
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3692
 
SmaI CCC'GGG

Cuts at:    445     714     445 
   Size:        269    3423
 
SmlI C'TyrA_G

Cuts at:    847    1762    2254    2516     847 
   Size:        915     492     262    2023

  Fragments arranged by size: 

                2023     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3692
 
SphI G_CATG'C

Cuts at:    745    1245     745 
   Size:        500    3192
 
StyI C'CwwG_G

Cuts at:    826    1180     826 
   Size:        354    3338
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1275    1408    1867    2076    1275 
   Size:        133     459     209    2891

  Fragments arranged by size: 

                2891     459     209     133
 
TatI w'GTAC_w

Cuts at:   3207    3207 
   Size:       3692
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3209    3241    3209 
   Size:         32    3660
 
Tth111I GACn'n_nGTC

Cuts at:   1489    1489 
   Size:       3692
 
XbaI T'CTAG_A

Cuts at:    723     723 
   Size:       3692
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    309    1187     309 
   Size:        878    2814
 
XmaI C'CCGG_G

Cuts at:    443     712     443 
   Size:        269    3423
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI    PflMI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI     BpmI
   Bpu10I    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI     ZraI
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