Restriction map for pF1KB7532
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        HaeII       HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MscI        MunI        
MvnI        NarI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tsp509I     Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccIII         24  TCCGGA               T^CCGGA
AfaI          576  GTAC                 GT^AC
AflIII        362  ACATGT               A^C[GA][CT]GT
AspHI         505  GAGCAC               G[AT]GC[AT]^C
AvaII         190  GGTCC                G^G[AT]CC
BanI          513  GGCACC               G^G[CT][GA]CC
BbsI          661  GAAGAC               GAAGAC(2/6)
BcgI          726  CGACGCCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI          493  CTGGAG               CTGGAG(16/14)
BpuAI         661  GAAGAC               GAAGAC(2/6)
BsaWI          24  TCCGGA               [AT]^CCGG[AT]
BseAI          24  TCCGGA               T^CCGGA
BsiHKAI       505  GAGCAC               G[AT]GC[AT]^C
BspEI          24  TCCGGA               T^CCGGA
BspLU11I      362  ACATGT               A^CATGT
BspMI         132  ACCTGC               ACCTGC(4/8)
BsrFI         253  GCCGGC               [GA]^CCGG[CT]
BstAPI        127  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI          736  GCGC                 GCG^C
Cfr10I        253  GCCGGC               [GA]^CCGG[CT]
EcoT14I       301  CCATGG               C^C[AT][AT]GG
HhaI          736  GCGC                 GCG^C
HinP1I        736  GCGC                 G^CGC
MroI           24  TCCGGA               T^CCGGA
MseI          740  TTAA                 T^TAA
NaeI          253  GCCGGC               GCC^GGC
NcoI          301  CCATGG               C^CATGG
NgoMIV        253  GCCGGC               G^CCGGC
NspI          362  ACATGT               [GA]CATG^[CT]
PpuMI         189  GGGTCCT              [GA]G^G[AT]CC[CT]
RsaI          576  GTAC                 GT^AC
SapI          671  GAAGAGC              GCTCTTC(1/4)
StyI          301  CCATGG               C^C[AT][AT]GG
TaqI          695  TCGA                 T^CGA
Tru9I         740  TTAA                 T^TAA
TthHB8I       695  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI           61  GGCGTC               G[GA]^CG[CT]C
              727  GACGCC              
BbvCI         538  CCTCAGC              CCTCAGC(-5/-2)
               93  GCTGAGG             
BsaHI          61  GGCGTC               G[GA]^CG[CT]C
              727  GACGCC              
BsgI          134  CTGCAC               GTGCAG(16/14)
              370  GTGCAG              
DsaI          301  CCATGG               C^C[GA][CT]GG
              532  CCGTGG              
EaeI           49  CGGCCA               [CT]^GGCC[GA]
              278  CGGCCA              
EarI          671  GAAGAG               CTCTTC(1/4)
              715  GAAGAG              
FauI          475  CCCGC                CCCGC(4/6)
              489  CCCGC               
HgaI          727  GACGC                GACGC(5/10)
               62  GCGTC               
Hin1I          61  GGCGTC               G[GA]^CG[CT]C
              727  GACGCC              
HincII        417  GTTGAC               GT[CT]^[GA]AC
              687  GTTGAC              
HindII        417  GTTGAC               GT[CT]^[GA]AC
              687  GTTGAC              
HphI          443  TCACC                GGTGA(8/7)
              521  TCACC               
Ksp632I       671  GAAGAG               CTCTTC(1/4)
              715  GAAGAG              
MaeIII        229  GTGAC                ^GT[ACGT]AC
              451  GTCAC               
PvuII         342  CAGCTG               CAG^CTG
              356  CAGCTG              
Tsp45I        229  GTGAC                ^GT[GC]AC
              451  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HpaII       ItaI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PstI        Sau96I      ScrFI       
SfcI        TseI        TspRI       XcmI        
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