Restriction map for pF1KB7532
 (Linear) (Six-base) MAPSORT of: KIBB7532.seq  Check: 1176  from: 1  to: 768



KIBB7532.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     141     768 
   Size:        141     627
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     768 
   Size:        634     134
 
AflIII A'CryG_T

Cuts at:      0     362     768 
   Size:        362     406
 
AleI CACnn'nnGTG

Cuts at:      0     184     397     768 
   Size:        184     213     371

  Fragments arranged by size: 

                 371     213     184
 
AlwNI CAG_nnn'CTG

Cuts at:      0     133     168     294     768 
   Size:        133      35     126     474

  Fragments arranged by size: 

                 474     133     126      35
 
ApaI G_GGCC'C

Cuts at:      0     407     488     502     608     768 
   Size:        407      81      14     106     160

  Fragments arranged by size: 

                 407     160     106      81      14
 
BanI G'GyrC_C

Cuts at:      0     513     768 
   Size:        513     255
 
BanII G_rGCy'C

Cuts at:      0     407     488     502     608     768 
   Size:        407      81      14     106     160

  Fragments arranged by size: 

                 407     160     106      81      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     668     768 
   Size:        668     100
 
BbvCI CC'TCA_GC

Cuts at:      0      94     539     768 
   Size:         94     445     229

  Fragments arranged by size: 

                 445     229      94
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     715     749     768 
   Size:        715      34      19

  Fragments arranged by size: 

                 715      34      19
 
Bme1580I G_kGCm'C

Cuts at:      0     329     385     407     488     502     608     768 
   Size:        329      56      22      81      14     106     160

  Fragments arranged by size: 

                 329     160     106      81      56      22      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     603     768 
   Size:        603     165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     768 
   Size:        514     254
 
Bpu10I CC'TnA_GC

Cuts at:      0      94     539     768 
   Size:         94     445     229

  Fragments arranged by size: 

                 445     229      94
 
BsaHI Gr'CG_yC

Cuts at:      0      62     728     768 
   Size:         62     666      40

  Fragments arranged by size: 

                 666      62      40
 
BsaWI w'CCGG_w

Cuts at:      0      24     768 
   Size:         24     744
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     111     250     399     768 
   Size:        111     139     149     369

  Fragments arranged by size: 

                 369     149     139     111
 
BseYI C'CCAG_C

Cuts at:      0      36     266     385     591     768 
   Size:         36     230     119     206     177

  Fragments arranged by size: 

                 230     206     177     119      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     391     768 
   Size:        119     272     377

  Fragments arranged by size: 

                 377     272     119
 
BsiHKAI G_wGCw'C

Cuts at:      0     509     768 
   Size:        509     259
 
Bsp1286I G_dGCh'C

Cuts at:      0     329     385     407     488     502     509     608     768 
   Size:        329      56      22      81      14       7      99     160

  Fragments arranged by size: 

                 329     160      99      81      56      22      14       7
            
 
BspEI T'CCGG_A

Cuts at:      0      24     768 
   Size:         24     744
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     141     768 
   Size:        141     627
 
BsrFI r'CCGG_y

Cuts at:      0     253     768 
   Size:        253     515
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     133     768 
   Size:        133     635
 
BtgI C'CryG_G

Cuts at:      0     301     532     768 
   Size:        301     231     236

  Fragments arranged by size: 

                 301     236     231
 
BtsI GCAGTG_nn'

Cuts at:      0     126     233     485     768 
   Size:        126     107     252     283

  Fragments arranged by size: 

                 283     252     126     107
 
EaeI y'GGCC_r

Cuts at:      0      49     278     768 
   Size:         49     229     490

  Fragments arranged by size: 

                 490     229      49
 
EarI CTCTTCn'nnn_

Cuts at:      0     666     710     768 
   Size:        666      44      58

  Fragments arranged by size: 

                 666      58      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     422     456     768 
   Size:        422      34     312

  Fragments arranged by size: 

                 422     312      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     634     768 
   Size:        514     120     134

  Fragments arranged by size: 

                 514     134     120
 
EcoO109I rG'GnC_Cy

Cuts at:      0     190     403     404     484     485     498     605     768 
   Size:        190     213       1      80       1      13     107     163

  Fragments arranged by size: 

                 213     190     163     107      80      13       1       1
            
 
HincII GTy'rAC

Cuts at:      0     419     689     768 
   Size:        419     270      79

  Fragments arranged by size: 

                 419     270      79
 
MslI CAynn'nnrTG

Cuts at:      0     184     367     397     768 
   Size:        184     183      30     371

  Fragments arranged by size: 

                 371     184     183      30
 
MspA1I CmG'CkG

Cuts at:      0     344     358     492     768 
   Size:        344      14     134     276

  Fragments arranged by size: 

                 344     276     134      14
 
NaeI GCC'GGC

Cuts at:      0     255     768 
   Size:        255     513
 
NcoI C'CATG_G

Cuts at:      0     301     768 
   Size:        301     467
 
NgoMIV G'CCGG_C

Cuts at:      0     253     768 
   Size:        253     515
 
NspI r_CATG'y

Cuts at:      0     366     768 
   Size:        366     402
 
PciI A'CATG_T

Cuts at:      0     362     768 
   Size:        362     406
 
PpuMI rG'GwC_Cy

Cuts at:      0     190     768 
   Size:        190     578
 
PspOMI G'GGCC_C

Cuts at:      0     403     484     498     604     768 
   Size:        403      81      14     106     164

  Fragments arranged by size: 

                 403     164     106      81      14
 
PstI C_TGCA'G

Cuts at:      0     228     290     575     768 
   Size:        228      62     285     193

  Fragments arranged by size: 

                 285     228     193      62
 
PvuII CAG'CTG

Cuts at:      0     344     358     768 
   Size:        344      14     410

  Fragments arranged by size: 

                 410     344      14
 
SapI GCTCTTCn'nnn_

Cuts at:      0     666     768 
   Size:        666     102
 
SfcI C'TryA_G

Cuts at:      0     224     286     571     768 
   Size:        224      62     285     197

  Fragments arranged by size: 

                 285     224     197      62
 
StyI C'CwwG_G

Cuts at:      0     301     768 
   Size:        301     467
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     174     206     768 
   Size:        174      32     562

  Fragments arranged by size: 

                 562     174      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     118     393     477     768 
   Size:        118     275      84     291

  Fragments arranged by size: 

                 291     275     118      84
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApaI     BanI    BanII
     BbsI    BbvCI     BcgI Bme1580I     BmrI     BpmI   Bpu10I    BsaHI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI
    BsrFI   BstAPI     BtgI     BtsI     EaeI     EarI     EciI  Eco57MI
 EcoO109I   HincII     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PciI    PpuMI   PspOMI     PstI    PvuII     SapI     SfcI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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