Restriction map for pF1KB7525
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StyI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        202  GGTACC               G^GTACC
AccI          148  GTAGAC               GT^[AC][GT]AC
AciI            4  GCGG                 CCGC(-3/-1)
AcsI          137  AAATTT               [GA]^AATT[CT]
AflIII        344  ACGTGT               A^C[GA][CT]GT
AhdI           67  GACCGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          111  GGATC                GGATC(4/5)
ApoI          137  AAATTT               [GA]^AATT[CT]
Asp718I       202  GGTACC               G^GTACC
AspEI          67  GACCGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          202  GGTACC               G^G[CT][GA]CC
BcnI           48  CCCGG                CC^[GC]GG
BpmI          414  CTCCAG               CTGGAG(16/14)
BsaAI         343  TACGTG               [CT]AC^GT[GA]
BsiWI         341  CGTACG               C^GTACG
BsiYI          12  CCTGTTTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           12  CCTGTTTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          75  GTCTC                GTCTC(1/5)
Bsp1407I      287  TGTACA               T^GTACA
BspHI         365  TCATGA               T^CATGA
BspMI         392  ACCTGC               ACCTGC(4/8)
BsrGI         287  TGTACA               T^GTACA
BsrI          258  CCAGT                ACTGG(1/-1)
BstYI         111  GGATCT               [GA]^GATC[CT]
Cfr13I        357  GGGCC                G^G[ACGT]CC
DdeI          120  CTTAG                C^T[ACGT]AG
DpnII         112  GATC                 ^GATC
DraII         356  GGGGCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I       67  GACCGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          12  CCTGTTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      356  GGGGCCT              [GA]G^G[ACGT]CC[CT]
FauI            4  GCGGG                CCCGC(4/6)
HapII          49  CCGG                 C^CGG
HpaII          49  CCGG                 C^CGG
KpnI          202  GGTACC               GGTAC^C
MaeII         344  ACGT                 A^CGT
MaeIII        158  GTGAC                ^GT[ACGT]AC
MboI          112  GATC                 ^GATC
MflI          111  GGATCT               [GA]^GATC[CT]
MspI           49  CCGG                 C^CGG
MwoI          363  GCTCATGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           48  CCCGG                CC^[GC]GG
NdeII         112  GATC                 ^GATC
NheI          441  GCTAGC               G^CTAGC
PleI          412  GACTC                GAGTC(4/5)
PstI          373  CTGCAG               CTGCA^G
RcaI          365  TCATGA               T^CATGA
Sau3AI        112  GATC                 ^GATC
Sau96I        357  GGGCC                G^G[ACGT]CC
SspBI         287  TGTACA               T^GTACA
StuI          241  AGGCCT               AGG^CCT
Tsp45I        158  GTGAC                ^GT[GC]AC
Tsp509I       138  AATT                 ^AATT
TspRI         231  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         111  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AluI          180  AGCT                 AG^CT
              440  AGCT                
BbvI          322  GCAGC                GCAGC(8/12)
              372  GCTGC               
BfaI          182  CTAG                 C^TAG
              442  CTAG                
BsaJI         206  CCCTGG               C^C[ACGT][ACGT]GG
              248  CCAGGG              
BstNI         207  CCTGG                CC^[AT]GG
              248  CCAGG               
EcoRII        207  CCTGG                ^CC[AT]GG
              248  CCAGG               
Fnu4HI        322  GCAGC                GC^[ACGT]GC
              372  GCTGC               
HaeIII        242  GGCC                 GG^CC
              358  GGCC                
HphI          157  GGTGA                GGTGA(8/7)
              217  GGTGA               
ItaI          322  GCAGC                GC^[ACGT]GC
              372  GCTGC               
MaeI          182  CTAG                 C^TAG
              442  CTAG                
MboII         196  GAAGA                GAAGA(8/7)
              435  GAAGA               
MvaI          207  CCTGG                CC^[AT]GG
              248  CCAGG               
NlaIII        366  CATG                 CATG^
              397  CATG                
NlaIV         202  GGTACC               GG[ACGT]^[ACGT]CC
              356  GGGGCC              
SfaNI         382  GATGC                GCATC(5/9)
                8  GCATC               
SfcI          146  CTGTAG               C^T[GA][CT]AG
              373  CTGCAG              
TfiI           97  GAATC                G^A[AT]TC
              401  GATTC               
TseI          322  GCAGC                G^C[AT]GC
              372  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AfaI        BssKI       BstF5I      Cac8I       FokI        
HinfI       MnlI        RsaI        ScrFI       TaqI        
TthHB8I     
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