Restriction map for pF1KB7523
##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvaI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AccII         358  CGCG                 CG^CG
Alw44I        399  GTGCAC               G^TGCAC
ApaLI         399  GTGCAC               G^TGCAC
AspHI         399  GTGCAC               G[AT]GC[AT]^C
BamHI         152  GGATCC               G^GATCC
BsiHKAI       399  GTGCAC               G[AT]GC[AT]^C
BsmI          314  GCATTC               GAATGC(1/-1)
BsrI           63  ACTGG                ACTGG(1/-1)
BstF5I         66  GGATG                GGATG(2/0)
BstUI         358  CGCG                 CG^CG
BstXI          34  CCAGATGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         152  GGATCC               [GA]^GATC[CT]
DraIII        404  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
FauI          170  CCCGC                CCCGC(4/6)
FokI           66  GGATG                GGATG(9/13)
HinfI         130  GAATC                G^A[ACGT]TC
MflI          152  GGATCC               [GA]^GATC[CT]
MseI          236  TTAA                 T^TAA
MvnI          358  CGCG                 CG^CG
NlaIII        109  CATG                 CATG^
NlaIV         152  GGATCC               GG[ACGT]^[ACGT]CC
SspI          227  AATATT               AAT^ATT
TfiI          130  GAATC                G^A[AT]TC
Tru9I         236  TTAA                 T^TAA
Tsp509I        71  AATT                 ^AATT
XcmI          365  CCAAAAGCAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         152  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AluI           93  AGCT                 AG^CT
              374  AGCT                
AlwI          153  GATCC                GGATC(4/5)
              152  GGATC               
BbvI          302  GCTGC                GCAGC(8/12)
              422  GCTGC               
BmyI          361  GTGCCC               G[AGT]GC[ACT]^C
              399  GTGCAC              
BsmFI         326  GGGAC                GGGAC(10/14)
              340  GGGAC               
Bsp1286I      361  GTGCCC               G[AGT]GC[ACT]^C
              399  GTGCAC              
Cfr13I        140  GGCCC                G^G[ACGT]CC
              384  GGGCC               
DpnII          76  GATC                 ^GATC
              153  GATC                
HaeIII        140  GGCC                 GG^CC
              385  GGCC                
HapII          21  CCGG                 C^CGG
              428  CCGG                
HpaII          21  CCGG                 C^CGG
              428  CCGG                
MboI           76  GATC                 ^GATC
              153  GATC                
MboII         146  TCTTC                GAAGA(8/7)
              179  TCTTC               
MspI           21  CCGG                 C^CGG
              428  CCGG                
NdeII          76  GATC                 ^GATC
              153  GATC                
Sau3AI         76  GATC                 ^GATC
              153  GATC                
Sau96I        140  GGCCC                G^G[ACGT]CC
              384  GGGCC               
SfaNI         350  GCATC                GCATC(5/9)
              389  GCATC               
TaqI          182  TCGA                 T^CGA
              395  TCGA                
TseI          302  GCTGC                G^C[AT]GC
              422  GCTGC               
TspRI          62  CACTG                CA[GC]TG[ACGT][ACGT]^
              406  CACTG               
TthHB8I       182  TCGA                 T^CGA
              395  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AciI        BsiYI       BslI        Cac8I       Fnu4HI      
ItaI        MnlI        MwoI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com