Restriction map for pF1KB7501
 (Circular) (Six-base) MAPSORT of: pF1KB7501.seq  Check: 6892  from: 1  to: 3287



pF1KB7501  3287 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    325     325 
   Size:       3287
 
Acc65I G'GTAC_C

Cuts at:    303     303 
   Size:       3287
 
AclI AA'CG_TT

Cuts at:   3127    3127 
   Size:       3287
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1112    1544    2291    2559    1112 
   Size:        432     747     268    1840

  Fragments arranged by size: 

                1840     747     432     268
 
AflIII A'CryG_T

Cuts at:    639    1743    2720     639 
   Size:       1104     977    1206

  Fragments arranged by size: 

                1206    1104     977
 
AgeI A'CCGG_T

Cuts at:   1675    1675 
   Size:       3287
 
AlwNI CAG_nnn'CTG

Cuts at:    592    2159     592 
   Size:       1567    1720
 
ApaLI G'TGCA_C

Cuts at:   2057    2057 
   Size:       3287
 
ApoI r'AATT_y

Cuts at:    291    2640    2896     291 
   Size:       2349     256     682

  Fragments arranged by size: 

                2349     682     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3287
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3287
 
AvaI C'yCGr_G

Cuts at:    278     307     278 
   Size:         29    3258
 
BamHI G'GATC_C

Cuts at:    201     312     201 
   Size:        111    3176
 
BanI G'GyrC_C

Cuts at:    303     717     965    1000     303 
   Size:        414     248      35    2590

  Fragments arranged by size: 

                2590     414     248      35
 
BanII G_rGCy'C

Cuts at:    301    1331     301 
   Size:       1030    2257
 
BbeI G_GCGC'C

Cuts at:    721     969     721 
   Size:        248    3039
 
BciVI GTATCCnnnnn_n'

Cuts at:    220    1185    1946    3287     220 
   Size:        965     761    1341     220

  Fragments arranged by size: 

                1341     965     761     220
 
BclI T'GATC_A

Cuts at:    807     807 
   Size:       3287
 
BglII A'GATC_T

Cuts at:    802    2839     802 
   Size:       2037    1250
 
BlpI GC'TnA_GC

Cuts at:    399     399 
   Size:       3287
 
Bme1580I G_kGCm'C

Cuts at:    912    1005    2061     912 
   Size:         93    1056    2138

  Fragments arranged by size: 

                2138    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    499     580     915    3059     499 
   Size:         81     335    2144     727

  Fragments arranged by size: 

                2144     727     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1654    1654 
   Size:       3287
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    463    1342    1834    2132     463 
   Size:        879     492     298    1618

  Fragments arranged by size: 

                1618     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2991    2991 
   Size:       3287
 
BsaAI yAC'GTr

Cuts at:    640     640 
   Size:       3287
 
BsaBI GATnn'nnATC

Cuts at:      6     157     801    2838       6 
   Size:        151     644    2037     455

  Fragments arranged by size: 

                2037     644     455     151
 
BsaHI Gr'CG_yC

Cuts at:    718     966    1668    3164     718 
   Size:        248     702    1496     841

  Fragments arranged by size: 

                1496     841     702     248
 
BsaWI w'CCGG_w

Cuts at:    476     997    1675    1949    2096    2507     476 
   Size:        521     678     274     147     411    1256

  Fragments arranged by size: 

                1256     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    712    1470    2047    2543     712 
   Size:        758     577     496    1456

  Fragments arranged by size: 

                1456     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     829     875    2083     104 
   Size:        725      46    1208    1308

  Fragments arranged by size: 

                1308    1208     725      46
 
BsiHKAI G_wGCw'C

Cuts at:    301    1079    1269    2061     301 
   Size:        778     190     792    1527

  Fragments arranged by size: 

                1527     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    301     912    1005    1079    1269    1331    2061     301 
   Size:        611      93      74     190      62     730    1527

  Fragments arranged by size: 

                1527     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2507    2507 
   Size:       3287
 
BspHI T'CATG_A

Cuts at:   2463    2463 
   Size:       3287
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    337     551     853    1234     337 
   Size:        214     302     381    2390

  Fragments arranged by size: 

                2390     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1579    1633    3117    3282      50 
   Size:       1529      54    1484     165      55

  Fragments arranged by size: 

                1529    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1199    1199 
   Size:       3287
 
BsrFI r'CCGG_y

Cuts at:   1285    1466    1675    1285 
   Size:        181     209    2897

  Fragments arranged by size: 

                2897     209     181
 
BsrGI T'GTAC_A

Cuts at:    239     239 
   Size:       3287
 
BssSI C'ACGA_G

Cuts at:   1558    1916    1558 
   Size:        358    2929
 
BstAPI GCAn_nnn'nTGC

Cuts at:    664     664 
   Size:       3287
 
BstBI TT'CG_AA

Cuts at:   1648    1648 
   Size:       3287
 
BstYI r'GATC_y

Cuts at:      1     201     312     779     802    1137    1383    2395    2481 
   Size:        200     111     467      23     335     246    1012      86

Cuts at:   2481    2493    2839       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     246     200     111
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3287
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1237    1418    1581    1237 
   Size:        181     163    2943

  Fragments arranged by size: 

                2943     181     163
 
BtsI GCAGTG_nn'

Cuts at:    530     530 
   Size:       3287
 
DraI TTT'AAA

Cuts at:    286     286 
   Size:       3287
 
DrdI GACnn_nn'nnGTC

Cuts at:    993    1851     993 
   Size:        858    2429
 
EaeI y'GGCC_r

Cuts at:    872    1046    1437    1464    2809     872 
   Size:        174     391      27    1345    1350

  Fragments arranged by size: 

                1350    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    872     872 
   Size:       3287
 
EarI CTCTTCn'nnn_

Cuts at:   1310    1520    1310 
   Size:        210    3077
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1495    1805    1951    3097    1495 
   Size:        310     146    1146    1685

  Fragments arranged by size: 

                1685    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    299     299 
   Size:       3287
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1112    1544    2291    2559    1112 
   Size:        432     747     268    1840

  Fragments arranged by size: 

                1840     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    426     426 
   Size:       3287
 
EcoRI G'AATT_C

Cuts at:    291     291 
   Size:       3287
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1306    1306 
   Size:       3287
 
FspI TGC'GCA

Cuts at:   1068    1068 
   Size:       3287
 
HaeII r_GCGC'y

Cuts at:    721     969    1991    2970     721 
   Size:        248    1022     979    1038

  Fragments arranged by size: 

                1038    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1129    1159    1161    1191    1129 
   Size:         30       2      30    3225

  Fragments arranged by size: 

                3225      30      30       2
 
HincII GTy'rAC

Cuts at:    326     326 
   Size:       3287
 
KasI G'GCGC_C

Cuts at:    717     965     717 
   Size:        248    3039
 
KpnI G_GTAC'C

Cuts at:    307     307 
   Size:       3287
 
MluI A'CGCG_T

Cuts at:   2720    2720 
   Size:       3287
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1958    2142    1958 
   Size:        184    3103
 
MscI TGG'CCA

Cuts at:   1048    1048 
   Size:       3287
 
MslI CAynn'nnrTG

Cuts at:   1276    1403    1276 
   Size:        127    3160
 
MspA1I CmG'CkG

Cuts at:    398     712    1072    2085    2330     398 
   Size:        314     360    1013     245    1355

  Fragments arranged by size: 

                1355    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1468    1468 
   Size:       3287
 
NarI GG'CG_CC

Cuts at:    718     966     718 
   Size:        248    3039
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3287
 
NgoMIV G'CCGG_C

Cuts at:   1466    1466 
   Size:       3287
 
NspI r_CATG'y

Cuts at:    340     840    1747     340 
   Size:        500     907    1880

  Fragments arranged by size: 

                1880     907     500
 
PciI A'CATG_T

Cuts at:   1743    1743 
   Size:       3287
 
PmeI GTTT'AAAC

Cuts at:    286     286 
   Size:       3287
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2426    2458    2426 
   Size:         32    3255
 
PspXI vC'TCGA_Gb

Cuts at:    278     278 
   Size:       3287
 
PstI C_TGCA'G

Cuts at:    334    1019     334 
   Size:        685    2602
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3287
 
PvuII CAG'CTG

Cuts at:    712    1072     712 
   Size:        360    2927
 
RsrII CG'GwC_CG

Cuts at:   1482    1482 
   Size:       3287
 
SacI G_AGCT'C

Cuts at:    301     301 
   Size:       3287
 
SalI G'TCGA_C

Cuts at:    324     324 
   Size:       3287
 
SapI GCTCTTCn'nnn_

Cuts at:   1310    1520    1310 
   Size:        210    3077
 
SbfI CC_TGCA'GG

Cuts at:    334     334 
   Size:       3287
 
ScaI AGT'ACT

Cuts at:   2804    2804 
   Size:       3287
 
SfcI C'TryA_G

Cuts at:     33     330    1015    2008    2199      33 
   Size:        297     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     297     191
 
SfoI GGC'GCC

Cuts at:    719     967     719 
   Size:        248    3039
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3287
 
SmaI CCC'GGG

Cuts at:    309     309 
   Size:       3287
 
SmlI C'TyrA_G

Cuts at:    278     442    1357    1849    2111     278 
   Size:        164     915     492     262    1454

  Fragments arranged by size: 

                1454     915     492     262     164
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3287
 
SphI G_CATG'C

Cuts at:    340     840     340 
   Size:        500    2787
 
StyI C'CwwG_G

Cuts at:    107     421     775     107 
   Size:        314     354    2619

  Fragments arranged by size: 

                2619     354     314
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    130     870    1003    1462    1671     130 
   Size:        740     133     459     209    1746

  Fragments arranged by size: 

                1746     740     459     209     133
 
TatI w'GTAC_w

Cuts at:    239    2802     239 
   Size:       2563     724
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2804    2836    2804 
   Size:         32    3255
 
Tth111I GACn'n_nGTC

Cuts at:   1084    1084 
   Size:       3287
 
XbaI T'CTAG_A

Cuts at:    318     318 
   Size:       3287
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    782     782 
   Size:       3287
 
XhoI C'TCGA_G

Cuts at:    278     278 
   Size:       3287
 
XmaI C'CCGG_G

Cuts at:    307     307 
   Size:       3287
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PmeI     PpiI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI
    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XmnI     ZraI
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