Restriction map for pF1KB7417
 (Circular) (Six-base) MAPSORT of: pF1KB7417.seq  Check: 1283  from: 1  to: 4208



pF1KB7417  4208 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    580     580 
   Size:       4208
 
AccI GT'mk_AC

Cuts at:   1246    1246 
   Size:       4208
 
Acc65I G'GTAC_C

Cuts at:   1224    1224 
   Size:       4208
 
AclI AA'CG_TT

Cuts at:   4048    4048 
   Size:       4208
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    581    2033    2465    3212    3480     581 
   Size:       1452     432     747     268    1309

  Fragments arranged by size: 

                1452    1309     747     432     268
 
AflIII A'CryG_T

Cuts at:   1560    2664    3641    1560 
   Size:       1104     977    2127

  Fragments arranged by size: 

                2127    1104     977
 
AgeI A'CCGG_T

Cuts at:   2596    2596 
   Size:       4208
 
AleI CACnn'nnGTG

Cuts at:    318     412     695    1180     318 
   Size:         94     283     485    3346

  Fragments arranged by size: 

                3346     485     283      94
 
AlwNI CAG_nnn'CTG

Cuts at:   1513    3080    1513 
   Size:       1567    2641
 
ApaLI G'TGCA_C

Cuts at:   2978    2978 
   Size:       4208
 
ApoI r'AATT_y

Cuts at:   1212    3561    3817    1212 
   Size:       2349     256    1603

  Fragments arranged by size: 

                2349    1603     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4208
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4208
 
AvaI C'yCGr_G

Cuts at:   1128    1228    1128 
   Size:        100    4108
 
BamHI G'GATC_C

Cuts at:   1233    1233 
   Size:       4208
 
BanI G'GyrC_C

Cuts at:    625    1224    1638    1886    1921     625 
   Size:        599     414     248      35    2912

  Fragments arranged by size: 

                2912     599     414     248      35
 
BanII G_rGCy'C

Cuts at:   1222    2252    1222 
   Size:       1030    3178
 
BbeI G_GCGC'C

Cuts at:   1642    1890    1642 
   Size:        248    3960
 
BbsI GAAGACnn'nnnn_

Cuts at:    780     780 
   Size:       4208
 
BciVI GTATCCnnnnn_n'

Cuts at:    128    2106    2867    4208     128 
   Size:       1978     761    1341     128

  Fragments arranged by size: 

                1978    1341     761     128
 
BclI T'GATC_A

Cuts at:   1728    1728 
   Size:       4208
 
BglII A'GATC_T

Cuts at:   1723    3760    1723 
   Size:       2037    2171
 
BlpI GC'TnA_GC

Cuts at:   1320    1320 
   Size:       4208
 
Bme1580I G_kGCm'C

Cuts at:    530    1833    1926    2982     530 
   Size:       1303      93    1056    1756

  Fragments arranged by size: 

                1756    1303    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    689    1420    1501    1836    3980     689 
   Size:        731      81     335    2144     917

  Fragments arranged by size: 

                2144     917     731     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1081    2575    1081 
   Size:       1494    2714
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    526     738    1105     526 
   Size:        212     367    3629

  Fragments arranged by size: 

                3629     367     212
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    449    1384    2263    2755    3053     449 
   Size:        935     879     492     298    1604

  Fragments arranged by size: 

                1604     935     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    845    3912     845 
   Size:       3067    1141
 
BsaAI yAC'GTr

Cuts at:    473    1164    1561     473 
   Size:        691     397    3120

  Fragments arranged by size: 

                3120     691     397
 
BsaBI GATnn'nnATC

Cuts at:      6    1722    3759       6 
   Size:       1716    2037     455

  Fragments arranged by size: 

                2037    1716     455
 
BsaHI Gr'CG_yC

Cuts at:   1639    1887    2589    4085    1639 
   Size:        248     702    1496    1762

  Fragments arranged by size: 

                1762    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1397    1918    2596    2870    3017    3428    1397 
   Size:        521     678     274     147     411    2177

  Fragments arranged by size: 

                2177     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    763     793    1141    1171     763 
   Size:         30     348      30    3800

  Fragments arranged by size: 

                3800     348      30      30
 
BseYI C'CCAG_C

Cuts at:   1168    1633    2391    2968    3464    1168 
   Size:        465     758     577     496    1912

  Fragments arranged by size: 

                1912     758     577     496     465
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1110    1204    1110 
   Size:         94    4114
 
BsiEI CG_ry'CG

Cuts at:    104    1750    1796    3004     104 
   Size:       1646      46    1208    1308

  Fragments arranged by size: 

                1646    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1222    2000    2190    2982    1222 
   Size:        778     190     792    2448

  Fragments arranged by size: 

                2448     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    530    1222    1833    1926    2000    2190    2252    2982     530 
   Size:        692     611      93      74     190      62     730    1756

  Fragments arranged by size: 

                1756     730     692     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3428    3428 
   Size:       4208
 
BspHI T'CATG_A

Cuts at:   3384    3384 
   Size:       4208
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    580    1258    1472    1774    2155     580 
   Size:        678     214     302     381    2633

  Fragments arranged by size: 

                2633     678     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2500    2554    4038    4203      50 
   Size:       2450      54    1484     165      55

  Fragments arranged by size: 

                2450    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1110    2120    1110 
   Size:       1010    3198
 
BsrFI r'CCGG_y

Cuts at:   2206    2387    2596    2206 
   Size:        181     209    3818

  Fragments arranged by size: 

                3818     209     181
 
BssSI C'ACGA_G

Cuts at:   2479    2837    2479 
   Size:        358    3850
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1121    1585    1121 
   Size:        464    3744
 
BstBI TT'CG_AA

Cuts at:   2569    2569 
   Size:       4208
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1168    1168 
   Size:       4208
 
BstYI r'GATC_y

Cuts at:      1     389    1233    1700    1723    2058    2304    3316    3402 
   Size:        388     844     467      23     335     246    1012      86

Cuts at:   3402    3414    3760       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     844     467     449     388     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    556     556 
   Size:       4208
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4208
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2158    2339    2502    2158 
   Size:        181     163    3864

  Fragments arranged by size: 

                3864     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1098    1451    1098 
   Size:        353    3855
 
DraI TTT'AAA

Cuts at:   1207    1207 
   Size:       4208
 
DrdI GACnn_nn'nnGTC

Cuts at:   1914    2772    1914 
   Size:        858    3350
 
EaeI y'GGCC_r

Cuts at:   1793    1967    2358    2385    3730    1793 
   Size:        174     391      27    1345    2271

  Fragments arranged by size: 

                2271    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1793    1793 
   Size:       4208
 
EarI CTCTTCn'nnn_

Cuts at:   2231    2441    2231 
   Size:        210    3998
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2416    2726    2872    4018    2416 
   Size:        310     146    1146    2606

  Fragments arranged by size: 

                2606    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1220    1220 
   Size:       4208
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    526     581     738    1105    2033    2465    3212    3480     526 
   Size:         55     157     367     928     432     747     268    1254

  Fragments arranged by size: 

                1254     928     747     432     367     268     157      55
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    505     505 
   Size:       4208
 
EcoO109I rG'GnC_Cy

Cuts at:   1347    1347 
   Size:       4208
 
EcoRI G'AATT_C

Cuts at:   1212    1212 
   Size:       4208
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2227    2227 
   Size:       4208
 
FspI TGC'GCA

Cuts at:   1989    1989 
   Size:       4208
 
HaeII r_GCGC'y

Cuts at:   1191    1642    1890    2912    3891    1191 
   Size:        451     248    1022     979    1508

  Fragments arranged by size: 

                1508    1022     979     451     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1092    1124    1141    1173    2050    2080    2082    2112    1092 
   Size:         32      17      32     877      30       2      30    3188

  Fragments arranged by size: 

                3188     877      32      32      30      30      17       2
            
 
HincII GTy'rAC

Cuts at:    185    1247     185 
   Size:       1062    3146
 
KasI G'GCGC_C

Cuts at:   1638    1886    1638 
   Size:        248    3960
 
KpnI G_GTAC'C

Cuts at:   1228    1228 
   Size:       4208
 
MluI A'CGCG_T

Cuts at:   3641    3641 
   Size:       4208
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1001    2879    3063    1001 
   Size:       1878     184    2146

  Fragments arranged by size: 

                2146    1878     184
 
MscI TGG'CCA

Cuts at:   1969    1969 
   Size:       4208
 
MslI CAynn'nnrTG

Cuts at:    318     412     695    1180    2197    2324     318 
   Size:         94     283     485    1017     127    2202

  Fragments arranged by size: 

                2202    1017     485     283     127      94
 
MspA1I CmG'CkG

Cuts at:    492    1319    1633    1993    3006    3251     492 
   Size:        827     314     360    1013     245    1449

  Fragments arranged by size: 

                1449    1013     827     360     314     245
 
NaeI GCC'GGC

Cuts at:   2389    2389 
   Size:       4208
 
NarI GG'CG_CC

Cuts at:   1639    1887    1639 
   Size:        248    3960
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4208
 
NdeI CA'TA_TG

Cuts at:    887     887 
   Size:       4208
 
NgoMIV G'CCGG_C

Cuts at:   2387    2387 
   Size:       4208
 
NspI r_CATG'y

Cuts at:   1261    1761    2668    1261 
   Size:        500     907    2801

  Fragments arranged by size: 

                2801     907     500
 
PciI A'CATG_T

Cuts at:   2664    2664 
   Size:       4208
 
PflMI CCAn_nnn'nTGG

Cuts at:    840     840 
   Size:       4208
 
PmeI GTTT'AAAC

Cuts at:   1207    1207 
   Size:       4208
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3347    3379    3347 
   Size:         32    4176
 
PstI C_TGCA'G

Cuts at:    481    1255    1940     481 
   Size:        774     685    2749

  Fragments arranged by size: 

                2749     774     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4208
 
PvuII CAG'CTG

Cuts at:    492    1633    1993     492 
   Size:       1141     360    2707

  Fragments arranged by size: 

                2707    1141     360
 
RsrII CG'GwC_CG

Cuts at:   2403    2403 
   Size:       4208
 
SacI G_AGCT'C

Cuts at:   1222    1222 
   Size:       4208
 
SalI G'TCGA_C

Cuts at:   1245    1245 
   Size:       4208
 
SapI GCTCTTCn'nnn_

Cuts at:   2231    2441    2231 
   Size:        210    3998
 
SbfI CC_TGCA'GG

Cuts at:   1255    1255 
   Size:       4208
 
ScaI AGT'ACT

Cuts at:   3725    3725 
   Size:       4208
 
SexAI A'CCwGG_T

Cuts at:    632     632 
   Size:       4208
 
SfcI C'TryA_G

Cuts at:     33     477    1251    1936    2929    3120      33 
   Size:        444     774     685     993     191    1121

  Fragments arranged by size: 

                1121     993     774     685     444     191
 
SfoI GGC'GCC

Cuts at:   1640    1888    1640 
   Size:        248    3960
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4208
 
SmaI CCC'GGG

Cuts at:   1130    1230    1130 
   Size:        100    4108
 
SmlI C'TyrA_G

Cuts at:    464    1363    2278    2770    3032     464 
   Size:        899     915     492     262    1640

  Fragments arranged by size: 

                1640     915     899     492     262
 
SnaBI TAC'GTA

Cuts at:    473     473 
   Size:       4208
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4208
 
SphI G_CATG'C

Cuts at:   1261    1761    1261 
   Size:        500    3708
 
SspI AAT'ATT

Cuts at:    298     933     298 
   Size:        635    3573
 
StuI AGG'CCT

Cuts at:    879     901     879 
   Size:         22    4186
 
StyI C'CwwG_G

Cuts at:    107     276     732    1342    1696     107 
   Size:        169     456     610     354    2619

  Fragments arranged by size: 

                2619     610     456     354     169
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    411     424    1791    1924    2383    2592     411 
   Size:         13    1367     133     459     209    2027

  Fragments arranged by size: 

                2027    1367     459     209     133      13
 
TatI w'GTAC_w

Cuts at:    569     836    3723     569 
   Size:        267    2887    1054

  Fragments arranged by size: 

                2887    1054     267
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    132     164    1164    1196    3725    3757     132 
   Size:         32    1000      32    2529      32     583

  Fragments arranged by size: 

                2529    1000     583      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2005    2005 
   Size:       4208
 
XbaI T'CTAG_A

Cuts at:   1239    1239 
   Size:       4208
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    582    1703     582 
   Size:       1121    3087
 
XmaI C'CCGG_G

Cuts at:   1128    1228    1128 
   Size:        100    4108
 
XmnI GAAnn'nnTTC

Cuts at:    286     933     286 
   Size:        647    3561
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
  BstZ17I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI     SfiI    SgrAI     SrfI     SwaI     XhoI     ZraI
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