(Linear) (Six-base) MAPSORT of: KIBB7417.seq  Check: 5786  from: 1  to: 1095



KIBB7417.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     472    1095 
   Size:        472     623
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473    1095 
   Size:        473     622
 
AleI CACnn'nnGTG

Cuts at:      0     210     304     587    1072    1095 
   Size:        210      94     283     485      23

  Fragments arranged by size: 

                 485     283     210      94      23
 
AvaI C'yCGr_G

Cuts at:      0    1020    1095 
   Size:       1020      75
 
BanI G'GyrC_C

Cuts at:      0     517    1095 
   Size:        517     578
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     672    1095 
   Size:        672     423
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      20    1095 
   Size:         20    1075
 
Bme1580I G_kGCm'C

Cuts at:      0     422    1095 
   Size:        422     673
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     581    1095 
   Size:        581     514
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     973    1095 
   Size:        973     122
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     630     997    1095 
   Size:        418     212     367      98

  Fragments arranged by size: 

                 418     367     212      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341    1095 
   Size:        341     754
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     737    1095 
   Size:        737     358
 
BsaAI yAC'GTr

Cuts at:      0     365    1056    1095 
   Size:        365     691      39

  Fragments arranged by size: 

                 691     365      39
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     655     685    1033    1063    1095 
   Size:        655      30     348      30      32

  Fragments arranged by size: 

                 655     348      32      30      30
 
BseYI C'CCAG_C

Cuts at:      0    1060    1095 
   Size:       1060      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1002    1095 
   Size:       1002      93
 
Bsp1286I G_dGCh'C

Cuts at:      0     422    1095 
   Size:        422     673
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     472    1095 
   Size:        472     623
 
BsrDI GCAATG_nn'

Cuts at:      0    1002    1095 
   Size:       1002      93
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1013    1095 
   Size:       1013      82
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1060    1095 
   Size:       1060      35
 
BstYI r'GATC_y

Cuts at:      0     281    1095 
   Size:        281     814
 
Bsu36I CC'TnA_GG

Cuts at:      0     448    1095 
   Size:        448     647
 
BtsI GCAGTG_nn'

Cuts at:      0     990    1095 
   Size:        990     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     473     630     997    1095 
   Size:        418      55     157     367      98

  Fragments arranged by size: 

                 418     367     157      98      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     397    1095 
   Size:        397     698
 
HaeII r_GCGC'y

Cuts at:      0    1083    1095 
   Size:       1083      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     984    1016    1033    1065    1095 
   Size:        984      32      17      32      30

  Fragments arranged by size: 

                 984      32      32      30      17
 
HincII GTy'rAC

Cuts at:      0      77    1095 
   Size:         77    1018
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     893    1095 
   Size:        893     202
 
MslI CAynn'nnrTG

Cuts at:      0     210     304     587    1072    1095 
   Size:        210      94     283     485      23

  Fragments arranged by size: 

                 485     283     210      94      23
 
MspA1I CmG'CkG

Cuts at:      0     384    1095 
   Size:        384     711
 
NdeI CA'TA_TG

Cuts at:      0     779    1095 
   Size:        779     316
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     732    1095 
   Size:        732     363
 
PstI C_TGCA'G

Cuts at:      0     373    1095 
   Size:        373     722
 
PvuII CAG'CTG

Cuts at:      0     384    1095 
   Size:        384     711
 
SexAI A'CCwGG_T

Cuts at:      0     524    1095 
   Size:        524     571
 
SfcI C'TryA_G

Cuts at:      0     369    1095 
   Size:        369     726
 
SmaI CCC'GGG

Cuts at:      0    1022    1095 
   Size:       1022      73
 
SmlI C'TyrA_G

Cuts at:      0     356    1095 
   Size:        356     739
 
SnaBI TAC'GTA

Cuts at:      0     365    1095 
   Size:        365     730
 
SspI AAT'ATT

Cuts at:      0     190     825    1095 
   Size:        190     635     270

  Fragments arranged by size: 

                 635     270     190
 
StuI AGG'CCT

Cuts at:      0     771     793    1095 
   Size:        771      22     302

  Fragments arranged by size: 

                 771     302      22
 
StyI C'CwwG_G

Cuts at:      0     168     624    1095 
   Size:        168     456     471

  Fragments arranged by size: 

                 471     456     168
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     303     316    1095 
   Size:        303      13     779

  Fragments arranged by size: 

                 779     303      13
 
TatI w'GTAC_w

Cuts at:      0     461     728    1095 
   Size:        461     267     367

  Fragments arranged by size: 

                 461     367     267
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      24      56    1056    1088    1095 
   Size:         24      32    1000      32       7

  Fragments arranged by size: 

                1000      32      32      24       7
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     474    1095 
   Size:        474     621
 
XmaI C'CCGG_G

Cuts at:      0    1020    1095 
   Size:       1020      75
 
XmnI GAAnn'nnTTC

Cuts at:      0     178     825    1095 
   Size:        178     647     270

  Fragments arranged by size: 

                 647     270     178
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AvaI     BanI     BbsI    BciVI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaXI    BseYI
     BsgI Bsp1286I    BspMI    BsrDI   BstAPI    BstXI    BstYI   Bsu36I
     BtsI  Eco57MI    EcoNI    HaeII    Hin4I   HincII     MmeI     MslI
   MspA1I     NdeI    PflMI     PstI    PvuII    SexAI     SfcI     SmaI
     SmlI    SnaBI     SspI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
   Bpu10I    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI
     ZraI