Restriction map for pF1KB7410
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI          502  AAATTC               [GA]^AATT[CT]
AfaI          390  GTAC                 GT^AC
AflIII        646  ACATGT               A^C[GA][CT]GT
AlwI          243  GGATC                GGATC(4/5)
AlwNI         142  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          502  AAATTC               [GA]^AATT[CT]
AvaI            9  CTCGGG               C^[CT]CG[GA]G
BfaI          700  CTAG                 C^TAG
BglII         688  AGATCT               A^GATCT
BpmI          247  CTCCAG               CTGGAG(16/14)
BsaBI         603  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI            6  GGTCTC               GGTCTC(1/5)
BsaWI         567  ACCGGA               [AT]^CCGG[AT]
BsmI          933  GAATGC               GAATGC(1/-1)
BsoBI           9  CTCGGG               C^[CT]CG[GA]G
BspLU11I      646  ACATGT               A^CATGT
BspMI         822  GCAGGT               ACCTGC(4/8)
BssSI         753  CTCGTG               CACGAG(-5/-1)
BstEII         90  GGTGACC              G^GT[ACGT]ACC
BstPI          90  GGTGACC              G^GT[ACGT]ACC
CfoI          697  GCGC                 GCG^C
DrdI          136  GACGGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          138  CGGCCA               [CT]^GGCC[GA]
EcoO65I        90  GGTGACC              G^GT[ACGT]ACC
EcoT14I       737  CCATGG               C^C[AT][AT]GG
HapII         568  CCGG                 C^CGG
HhaI          697  GCGC                 GCG^C
HinP1I        697  GCGC                 G^CGC
HpaII         568  CCGG                 C^CGG
MaeI          700  CTAG                 C^TAG
MamI          603  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        890  CAGCTG               C[AC]G^C[GT]G
MspI          568  CCGG                 C^CGG
NcoI          737  CCATGG               C^CATGG
PleI          779  GACTC                GAGTC(4/5)
PpuMI         143  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         890  CAGCTG               CAG^CTG
RsaI          390  GTAC                 GT^AC
ScaI          389  AGTACT               AGT^ACT
SphI          398  GCATGC               GCATG^C
StyI          737  CCATGG               C^C[AT][AT]GG
TaqI          929  TCGA                 T^CGA
TfiI          200  GAATC                G^A[AT]TC
Tsp509I       503  AATT                 ^AATT
TthHB8I       929  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AciI          519  CCGC                 CCGC(-3/-1)
              581  CCGC                
Asp700I       619  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              933  GAATGCCTTC          
AspHI         190  GAGCTC               G[AT]GC[AT]^C
              312  GAGCTC              
BanI           14  GGCACC               G^G[CT][GA]CC
              539  GGTGCC              
BanII         190  GAGCTC               G[GA]GC[CT]^C
              312  GAGCTC              
BciVI         981  GGATAC               GTATCC(6/5)
              419  GTATCC              
BsgI          330  CTGCAC               GTGCAG(16/14)
              887  GTGCAG              
BsiHKAI       190  GAGCTC               G[AT]GC[AT]^C
              312  GAGCTC              
BsmAI         913  GAGAC                GTCTC(1/5)
                7  GTCTC               
BsmFI         595  GTCCC                GGGAC(10/14)
              758  GTCCC               
BsrDI         855  CATTGC               GCAATG(2/0)
             1056  CATTGC              
BsrI          299  ACTGG                ACTGG(1/-1)
              765  ACTGG               
BstYI         243  GGATCT               [GA]^GATC[CT]
              688  AGATCT              
DsaI          347  CCGTGG               C^C[GA][CT]GG
              737  CCATGG              
FauI          518  CCCGC                CCCGC(4/6)
              580  CCCGC               
HinfI         200  GAATC                G^A[ACGT]TC
              779  GACTC               
MflI          243  GGATCT               [GA]^GATC[CT]
              688  AGATCT              
MseI          284  TTAA                 T^TAA
             1145  TTAA                
NspI          398  GCATGC               [GA]CATG^[CT]
              646  ACATGT              
PflMI         141  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              293  CCACACACTGG         
SacI          190  GAGCTC               GAGCT^C
              312  GAGCTC              
SapI          619  GAAGAGC              GCTCTTC(1/4)
               57  GCTCTTC             
SfaNI          32  GATGC                GCATC(5/9)
              255  GCATC               
Tru9I         284  TTAA                 T^TAA
             1145  TTAA                
Van91I        141  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              293  CCACACACTGG         
XhoII         243  GGATCT               [GA]^GATC[CT]
              688  AGATCT              
XmnI          619  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              933  GAATGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AluI        AvaII       BbsI        BbvI        BmyI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PstI        Sau3AI      Sau96I      
ScrFI       SfcI        TseI        Tsp45I      TspRI       
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