Restriction map for pF1KB7410
 (Linear) (Six-base) MAPSORT of: KIBB7410.seq  Check: 7742  from: 1  to: 1152



KIBB7410.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     813    1152 
   Size:        813     339
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     638    1152 
   Size:        638     514
 
AflIII A'CryG_T

Cuts at:      0     646    1152 
   Size:        646     506
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1036    1068    1152 
   Size:       1036      32      84

  Fragments arranged by size: 

                1036      84      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     147    1152 
   Size:        147    1005
 
ApoI r'AATT_y

Cuts at:      0     502    1152 
   Size:        502     650
 
AvaI C'yCGr_G

Cuts at:      0       9    1152 
   Size:          9    1143
 
BanI G'GyrC_C

Cuts at:      0      14     539    1152 
   Size:         14     525     613

  Fragments arranged by size: 

                 613     525      14
 
BanII G_rGCy'C

Cuts at:      0     194     316    1152 
   Size:        194     122     836

  Fragments arranged by size: 

                 836     194     122
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     111     236     783    1152 
   Size:        111     125     547     369

  Fragments arranged by size: 

                 547     369     125     111
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     430     975    1152 
   Size:        430     545     177

  Fragments arranged by size: 

                 545     430     177
 
BglII A'GATC_T

Cuts at:      0     688    1152 
   Size:        688     464
 
Bme1580I G_kGCm'C

Cuts at:      0      17     544    1152 
   Size:         17     527     608

  Fragments arranged by size: 

                 608     527      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232    1152 
   Size:        232     920
 
Bpu10I CC'TnA_GC

Cuts at:      0     310    1152 
   Size:        310     842
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12    1152 
   Size:         12    1140
 
BsaBI GATnn'nnATC

Cuts at:      0     607    1152 
   Size:        607     545
 
BsaWI w'CCGG_w

Cuts at:      0     567    1152 
   Size:        567     585
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     230     260     904     934    1036    1066    1152 
   Size:        230      30     644      30     102      30      86

  Fragments arranged by size: 

                 644     230     102      86      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     517     759    1152 
   Size:        202     315     242     393

  Fragments arranged by size: 

                 393     315     242     202
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315     908    1152 
   Size:        315     593     244

  Fragments arranged by size: 

                 593     315     244
 
BsiHKAI G_wGCw'C

Cuts at:      0     194     316    1152 
   Size:        194     122     836

  Fragments arranged by size: 

                 836     194     122
 
BsmI GAATG_Cn'

Cuts at:      0     939    1152 
   Size:        939     213
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     194     316     544    1152 
   Size:         17     177     122     228     608

  Fragments arranged by size: 

                 608     228     177     122      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     813    1152 
   Size:        813     339
 
BsrDI GCAATG_nn'

Cuts at:      0     854    1055    1152 
   Size:        854     201      97

  Fragments arranged by size: 

                 854     201      97
 
BssSI C'ACGA_G

Cuts at:      0     753    1152 
   Size:        753     399
 
BstEII G'GTnAC_C

Cuts at:      0      90    1152 
   Size:         90    1062
 
BstYI r'GATC_y

Cuts at:      0     243     688    1152 
   Size:        243     445     464

  Fragments arranged by size: 

                 464     445     243
 
BtgI C'CryG_G

Cuts at:      0     347     737    1152 
   Size:        347     390     415

  Fragments arranged by size: 

                 415     390     347
 
BtsI GCAGTG_nn'

Cuts at:      0     219     715    1152 
   Size:        219     496     437

  Fragments arranged by size: 

                 496     437     219
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     142    1152 
   Size:        142    1010
 
EaeI y'GGCC_r

Cuts at:      0     138    1152 
   Size:        138    1014
 
EarI CTCTTCn'nnn_

Cuts at:      0      64     559     614    1152 
   Size:         64     495      55     538

  Fragments arranged by size: 

                 538     495      64      55
 
EcoICRI GAG'CTC

Cuts at:      0     192     314    1152 
   Size:        192     122     838

  Fragments arranged by size: 

                 838     192     122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     638    1152 
   Size:        232     406     514

  Fragments arranged by size: 

                 514     406     232
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     144     631    1152 
   Size:         45      99     487     521

  Fragments arranged by size: 

                 521     487      99      45
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     795     815     902    1017    1152 
   Size:        795      20      87     115     135

  Fragments arranged by size: 

                 795     135     115      87      20
 
MspA1I CmG'CkG

Cuts at:      0     892    1152 
   Size:        892     260
 
NcoI C'CATG_G

Cuts at:      0     737    1152 
   Size:        737     415
 
NspI r_CATG'y

Cuts at:      0     402     650    1152 
   Size:        402     248     502

  Fragments arranged by size: 

                 502     402     248
 
PasI CC'CwG_GG

Cuts at:      0      48    1152 
   Size:         48    1104
 
PciI A'CATG_T

Cuts at:      0     646    1152 
   Size:        646     506
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     147     299    1152 
   Size:        147     152     853

  Fragments arranged by size: 

                 853     152     147
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1036    1068    1152 
   Size:       1036      32      84

  Fragments arranged by size: 

                1036      84      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     144    1152 
   Size:        144    1008
 
PsiI TTA'TAA

Cuts at:      0     488    1152 
   Size:        488     664
 
PstI C_TGCA'G

Cuts at:      0     199     214     263     670    1152 
   Size:        199      15      49     407     482

  Fragments arranged by size: 

                 482     407     199      49      15
 
PvuII CAG'CTG

Cuts at:      0     892    1152 
   Size:        892     260
 
SacI G_AGCT'C

Cuts at:      0     194     316    1152 
   Size:        194     122     836

  Fragments arranged by size: 

                 836     194     122
 
SapI GCTCTTCn'nnn_

Cuts at:      0      64     614    1152 
   Size:         64     550     538

  Fragments arranged by size: 

                 550     538      64
 
ScaI AGT'ACT

Cuts at:      0     391    1152 
   Size:        391     761
 
SfcI C'TryA_G

Cuts at:      0     195     210     259     666    1152 
   Size:        195      15      49     407     486

  Fragments arranged by size: 

                 486     407     195      49      15
 
SphI G_CATG'C

Cuts at:      0     402    1152 
   Size:        402     750
 
StyI C'CwwG_G

Cuts at:      0     737    1152 
   Size:        737     415
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      97    1152 
   Size:         97    1055
 
TatI w'GTAC_w

Cuts at:      0     389    1152 
   Size:        389     763
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     809     841    1152 
   Size:        809      32     311

  Fragments arranged by size: 

                 809     311      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     623     937    1152 
   Size:        623     314     215

  Fragments arranged by size: 

                 623     314     215
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AloI    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI    BciVI    BglII Bme1580I     BpmI   Bpu10I     BsaI
    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BssSI   BstEII    BstYI     BtgI     BtsI     DrdI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     MmeI   MspA1I     NcoI
     NspI     PasI     PciI    PflMI     PpiI    PpuMI     PsiI     PstI
    PvuII     SacI     SapI     ScaI     SfcI     SphI     StyI    TaqII
     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaHI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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