(Circular) (Six-base) MAPSORT of: pF1KB7403.seq Check: 476 from: 1 to: 4583 pF1KB7403 4583 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AccI GT'mk_AC Cuts at: 917 1294 1621 917 Size: 377 327 3879 Fragments arranged by size: 3879 377 327 Acc65I G'GTAC_C Cuts at: 1599 1599 Size: 4583 AclI AA'CG_TT Cuts at: 4423 4423 Size: 4583 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 553 1232 2408 2840 3587 3855 553 Size: 679 1176 432 747 268 1281 Fragments arranged by size: 1281 1176 747 679 432 268 AflIII A'CryG_T Cuts at: 1935 3039 4016 1935 Size: 1104 977 2502 Fragments arranged by size: 2502 1104 977 AgeI A'CCGG_T Cuts at: 2971 2971 Size: 4583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1148 1148 Size: 4583 AlwNI CAG_nnn'CTG Cuts at: 1070 1888 3455 1070 Size: 818 1567 2198 Fragments arranged by size: 2198 1567 818 ApaLI G'TGCA_C Cuts at: 3353 3353 Size: 4583 ApoI r'AATT_y Cuts at: 310 663 714 854 1276 1587 3936 4192 310 Size: 353 51 140 422 311 2349 256 701 Fragments arranged by size: 2349 701 422 353 311 256 140 51 AseI AT'TA_AT Cuts at: 20 20 Size: 4583 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4583 AvaI C'yCGr_G Cuts at: 1603 1603 Size: 4583 BamHI G'GATC_C Cuts at: 685 1608 685 Size: 923 3660 BanI G'GyrC_C Cuts at: 961 1599 2013 2261 2296 961 Size: 638 414 248 35 3248 Fragments arranged by size: 3248 638 414 248 35 BanII G_rGCy'C Cuts at: 1597 2627 1597 Size: 1030 3553 BbeI G_GCGC'C Cuts at: 2017 2265 2017 Size: 248 4335 BbsI GAAGACnn'nnnn_ Cuts at: 922 922 Size: 4583 BciVI GTATCCnnnnn_n' Cuts at: 2481 3242 4583 2481 Size: 761 1341 2481 Fragments arranged by size: 2481 1341 761 BclI T'GATC_A Cuts at: 2103 2103 Size: 4583 BglII A'GATC_T Cuts at: 2098 4135 2098 Size: 2037 2546 BlpI GC'TnA_GC Cuts at: 1695 1695 Size: 4583 Bme1580I G_kGCm'C Cuts at: 567 2208 2301 3357 567 Size: 1641 93 1056 1793 Fragments arranged by size: 1793 1641 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1795 1876 2211 4355 1795 Size: 81 335 2144 2023 Fragments arranged by size: 2144 2023 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2950 2950 Size: 4583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 762 1290 762 Size: 528 4055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1759 2638 3130 3428 1759 Size: 879 492 298 2914 Fragments arranged by size: 2914 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4287 4287 Size: 4583 BsaAI yAC'GTr Cuts at: 1936 1936 Size: 4583 BsaBI GATnn'nnATC Cuts at: 6 690 2097 4134 6 Size: 684 1407 2037 455 Fragments arranged by size: 2037 1407 684 455 BsaHI Gr'CG_yC Cuts at: 2014 2262 2964 4460 2014 Size: 248 702 1496 2137 Fragments arranged by size: 2137 1496 702 248 BsaWI w'CCGG_w Cuts at: 1772 2293 2971 3245 3392 3803 1772 Size: 521 678 274 147 411 2552 Fragments arranged by size: 2552 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1208 1238 1208 Size: 30 4553 BseYI C'CCAG_C Cuts at: 1499 2008 2766 3343 3839 1499 Size: 509 758 577 496 2243 Fragments arranged by size: 2243 758 577 509 496 BsiEI CG_ry'CG Cuts at: 104 2125 2171 3379 104 Size: 2021 46 1208 1308 Fragments arranged by size: 2021 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1597 2375 2565 3357 1597 Size: 778 190 792 2823 Fragments arranged by size: 2823 792 778 190 Bsp1286I G_dGCh'C Cuts at: 567 1597 2208 2301 2375 2565 2627 3357 567 Size: 1030 611 93 74 190 62 730 1793 Fragments arranged by size: 1793 1030 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3803 3803 Size: 4583 BspHI T'CATG_A Cuts at: 342 3759 342 Size: 3417 1166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 588 916 1116 1633 1847 2149 2530 588 Size: 328 200 517 214 302 381 2641 Fragments arranged by size: 2641 517 381 328 302 214 200 BsrBI CCG'CTC Cuts at: 50 2875 2929 4413 4578 50 Size: 2825 54 1484 165 55 Fragments arranged by size: 2825 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1263 2495 1263 Size: 1232 3351 BsrFI r'CCGG_y Cuts at: 593 2581 2762 2971 593 Size: 1988 181 209 2205 Fragments arranged by size: 2205 1988 209 181 BssSI C'ACGA_G Cuts at: 2854 3212 2854 Size: 358 4225 BstAPI GCAn_nnn'nTGC Cuts at: 1960 1960 Size: 4583 BstBI TT'CG_AA Cuts at: 2944 2944 Size: 4583 BstXI CCAn_nnnn'nTGG Cuts at: 681 681 Size: 4583 BstYI r'GATC_y Cuts at: 1 685 1608 2075 2098 2433 2679 3691 3777 Size: 684 923 467 23 335 246 1012 86 Cuts at: 3777 3789 4135 1 Size: 12 346 449 Fragments arranged by size: 1012 923 684 467 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 918 918 Size: 4583 Bsu36I CC'TnA_GG Cuts at: 581 581 Size: 4583 BtgI C'CryG_G Cuts at: 107 107 Size: 4583 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2533 2714 2877 2533 Size: 181 163 4239 Fragments arranged by size: 4239 181 163 BtsI GCAGTG_nn' Cuts at: 1826 1826 Size: 4583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 971 1006 971 Size: 35 4548 DraI TTT'AAA Cuts at: 1265 1582 1265 Size: 317 4266 DrdI GACnn_nn'nnGTC Cuts at: 2289 3147 2289 Size: 858 3725 EaeI y'GGCC_r Cuts at: 773 2168 2342 2733 2760 4105 773 Size: 1395 174 391 27 1345 1251 Fragments arranged by size: 1395 1345 1251 391 174 27 EagI C'GGCC_G Cuts at: 2168 2168 Size: 4583 EarI CTCTTCn'nnn_ Cuts at: 1364 2606 2816 1364 Size: 1242 210 3131 Fragments arranged by size: 3131 1242 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2791 3101 3247 4393 2791 Size: 310 146 1146 2981 Fragments arranged by size: 2981 1146 310 146 EcoICRI GAG'CTC Cuts at: 1595 1595 Size: 4583 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 553 762 1232 1290 2408 2840 3587 3855 553 Size: 209 470 58 1118 432 747 268 1281 Fragments arranged by size: 1281 1118 747 470 432 268 209 58 EcoO109I rG'GnC_Cy Cuts at: 414 577 1722 414 Size: 163 1145 3275 Fragments arranged by size: 3275 1145 163 EcoRI G'AATT_C Cuts at: 1276 1587 1276 Size: 311 4272 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2602 2602 Size: 4583 FspI TGC'GCA Cuts at: 2364 2364 Size: 4583 HaeII r_GCGC'y Cuts at: 2017 2265 3287 4266 2017 Size: 248 1022 979 2334 Fragments arranged by size: 2334 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 670 702 2425 2455 2457 2487 670 Size: 32 1723 30 2 30 2766 Fragments arranged by size: 2766 1723 32 30 30 2 HincII GTy'rAC Cuts at: 1622 1622 Size: 4583 HindIII A'AGCT_T Cuts at: 768 768 Size: 4583 KasI G'GCGC_C Cuts at: 2013 2261 2013 Size: 248 4335 KpnI G_GTAC'C Cuts at: 1603 1603 Size: 4583 MfeI C'AATT_G Cuts at: 1427 1427 Size: 4583 MluI A'CGCG_T Cuts at: 4016 4016 Size: 4583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 971 3254 3438 971 Size: 2283 184 2116 Fragments arranged by size: 2283 2116 184 MscI TGG'CCA Cuts at: 775 2344 775 Size: 1569 3014 MslI CAynn'nnrTG Cuts at: 347 821 1573 2572 2699 347 Size: 474 752 999 127 2231 Fragments arranged by size: 2231 999 752 474 127 MspA1I CmG'CkG Cuts at: 940 1694 2008 2368 3381 3626 940 Size: 754 314 360 1013 245 1897 Fragments arranged by size: 1897 1013 754 360 314 245 NaeI GCC'GGC Cuts at: 595 2764 595 Size: 2169 2414 NarI GG'CG_CC Cuts at: 2014 2262 2014 Size: 248 4335 NcoI C'CATG_G Cuts at: 107 107 Size: 4583 NdeI CA'TA_TG Cuts at: 822 822 Size: 4583 NgoMIV G'CCGG_C Cuts at: 593 2762 593 Size: 2169 2414 NspI r_CATG'y Cuts at: 1636 2136 3043 1636 Size: 500 907 3176 Fragments arranged by size: 3176 907 500 PciI A'CATG_T Cuts at: 3039 3039 Size: 4583 PflMI CCAn_nnn'nTGG Cuts at: 1518 1518 Size: 4583 PmeI GTTT'AAAC Cuts at: 1582 1582 Size: 4583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1530 1562 3722 3754 1530 Size: 32 2160 32 2359 Fragments arranged by size: 2359 2160 32 32 PpuMI rG'GwC_Cy Cuts at: 414 577 414 Size: 163 4420 PstI C_TGCA'G Cuts at: 1630 2315 1630 Size: 685 3898 PvuI CG_AT'CG Cuts at: 104 104 Size: 4583 PvuII CAG'CTG Cuts at: 940 2008 2368 940 Size: 1068 360 3155 Fragments arranged by size: 3155 1068 360 RsrII CG'GwC_CG Cuts at: 2778 2778 Size: 4583 SacI G_AGCT'C Cuts at: 1597 1597 Size: 4583 SalI G'TCGA_C Cuts at: 1620 1620 Size: 4583 SapI GCTCTTCn'nnn_ Cuts at: 2606 2816 2606 Size: 210 4373 SbfI CC_TGCA'GG Cuts at: 1630 1630 Size: 4583 ScaI AGT'ACT Cuts at: 1326 1375 4100 1326 Size: 49 2725 1809 Fragments arranged by size: 2725 1809 49 SexAI A'CCwGG_T Cuts at: 997 997 Size: 4583 SfcI C'TryA_G Cuts at: 33 203 860 887 1062 1377 1626 2311 3304 Size: 170 657 27 175 315 249 685 993 Cuts at: 3304 3495 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 657 315 249 191 175 170 27 SfoI GGC'GCC Cuts at: 2015 2263 2015 Size: 248 4335 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4583 SmaI CCC'GGG Cuts at: 1605 1605 Size: 4583 SmlI C'TyrA_G Cuts at: 1738 2653 3145 3407 1738 Size: 915 492 262 2914 Fragments arranged by size: 2914 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4583 SphI G_CATG'C Cuts at: 1636 2136 1636 Size: 500 4083 SspI AAT'ATT Cuts at: 374 502 621 1140 374 Size: 128 119 519 3817 Fragments arranged by size: 3817 519 128 119 StyI C'CwwG_G Cuts at: 107 985 1209 1717 2071 107 Size: 878 224 508 354 2619 Fragments arranged by size: 2619 878 508 354 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2166 2299 2758 2967 2166 Size: 133 459 209 3782 Fragments arranged by size: 3782 459 209 133 TatI w'GTAC_w Cuts at: 1324 1373 4098 1324 Size: 49 2725 1809 Fragments arranged by size: 2725 1809 49 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4100 4132 4100 Size: 32 4551 Tth111I GACn'n_nGTC Cuts at: 2380 2380 Size: 4583 XbaI T'CTAG_A Cuts at: 1614 1614 Size: 4583 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2078 2078 Size: 4583 XmaI C'CCGG_G Cuts at: 1603 1603 Size: 4583 XmnI GAAnn'nnTTC Cuts at: 314 314 Size: 4583 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII ClaI DraIII EcoNI EcoRV FseI FspAI HpaI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI