Restriction map for pF1KB7391
 (Circular) (Six-base) MAPSORT of: pF1KB7391.seq  Check: 2692  from: 1  to: 3806



pF1KB7391  3806 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    586     586 
   Size:       3806
 
AccI GT'mk_AC

Cuts at:    844     844 
   Size:       3806
 
Acc65I G'GTAC_C

Cuts at:    822     822 
   Size:       3806
 
AclI AA'CG_TT

Cuts at:   3646    3646 
   Size:       3806
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    154    1631    2063    2810    3078     154 
   Size:       1477     432     747     268     882

  Fragments arranged by size: 

                1477     882     747     432     268
 
AflIII A'CryG_T

Cuts at:    388    1158    2262    3239     388 
   Size:        770    1104     977     955

  Fragments arranged by size: 

                1104     977     955     770
 
AgeI A'CCGG_T

Cuts at:   2194    2194 
   Size:       3806
 
AlwNI CAG_nnn'CTG

Cuts at:    462    1111    2678     462 
   Size:        649    1567    1590

  Fragments arranged by size: 

                1590    1567     649
 
ApaLI G'TGCA_C

Cuts at:   2576    2576 
   Size:       3806
 
ApoI r'AATT_y

Cuts at:    810    3159    3415     810 
   Size:       2349     256    1201

  Fragments arranged by size: 

                2349    1201     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3806
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3806
 
AvaI C'yCGr_G

Cuts at:    826     826 
   Size:       3806
 
AvrII C'CTAG_G

Cuts at:    337     337 
   Size:       3806
 
BamHI G'GATC_C

Cuts at:    831     831 
   Size:       3806
 
BanI G'GyrC_C

Cuts at:    795     822    1236    1484    1519     795 
   Size:         27     414     248      35    3082

  Fragments arranged by size: 

                3082     414     248      35      27
 
BanII G_rGCy'C

Cuts at:    820    1850     820 
   Size:       1030    2776
 
BbeI G_GCGC'C

Cuts at:   1240    1488    1240 
   Size:        248    3558
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    564     598     564 
   Size:         34    3772
 
BciVI GTATCCnnnnn_n'

Cuts at:   1704    2465    3806    1704 
   Size:        761    1341    1704

  Fragments arranged by size: 

                1704    1341     761
 
BclI T'GATC_A

Cuts at:   1326    1326 
   Size:       3806
 
BfrBI ATG'CAT

Cuts at:    529     529 
   Size:       3806
 
BglII A'GATC_T

Cuts at:   1321    3358    1321 
   Size:       2037    1769
 
BlpI GC'TnA_GC

Cuts at:    918     918 
   Size:       3806
 
Bme1580I G_kGCm'C

Cuts at:    800    1431    1524    2580     800 
   Size:        631      93    1056    2026

  Fragments arranged by size: 

                2026    1056     631      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1018    1099    1434    3578    1018 
   Size:         81     335    2144    1246

  Fragments arranged by size: 

                2144    1246     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2173    2173 
   Size:       3806
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    698     698 
   Size:       3806
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    982    1861    2353    2651     982 
   Size:        879     492     298    2137

  Fragments arranged by size: 

                2137     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    161    3510     161 
   Size:       3349     457
 
BsaAI yAC'GTr

Cuts at:   1159    1159 
   Size:       3806
 
BsaBI GATnn'nnATC

Cuts at:      6    1320    3357       6 
   Size:       1314    2037     455

  Fragments arranged by size: 

                2037    1314     455
 
BsaHI Gr'CG_yC

Cuts at:   1237    1485    2187    3683    1237 
   Size:        248     702    1496    1360

  Fragments arranged by size: 

                1496    1360     702     248
 
BsaWI w'CCGG_w

Cuts at:    995    1516    2194    2468    2615    3026     995 
   Size:        521     678     274     147     411    1775

  Fragments arranged by size: 

                1775     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    523     523 
   Size:       3806
 
BseYI C'CCAG_C

Cuts at:   1231    1989    2566    3062    1231 
   Size:        758     577     496    1975

  Fragments arranged by size: 

                1975     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    261     396     261 
   Size:        135    3671
 
BsiEI CG_ry'CG

Cuts at:    104    1348    1394    2602     104 
   Size:       1244      46    1208    1308

  Fragments arranged by size: 

                1308    1244    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    820    1598    1788    2580     820 
   Size:        778     190     792    2046

  Fragments arranged by size: 

                2046     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    208     208 
   Size:       3806
 
Bsp1286I G_dGCh'C

Cuts at:    800     820    1431    1524    1598    1788    1850    2580     800 
   Size:         20     611      93      74     190      62     730    2026

  Fragments arranged by size: 

                2026     730     611     190      93      74      62      20
            
 
BspEI T'CCGG_A

Cuts at:   3026    3026 
   Size:       3806
 
BspHI T'CATG_A

Cuts at:   2982    2982 
   Size:       3806
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    586     856    1070    1372    1753     586 
   Size:        270     214     302     381    2639

  Fragments arranged by size: 

                2639     381     302     270     214
 
BsrBI CCG'CTC

Cuts at:     50    2098    2152    3636    3801      50 
   Size:       2048      54    1484     165      55

  Fragments arranged by size: 

                2048    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1718    1718 
   Size:       3806
 
BsrFI r'CCGG_y

Cuts at:    562    1804    1985    2194     562 
   Size:       1242     181     209    2174

  Fragments arranged by size: 

                2174    1242     209     181
 
BssSI C'ACGA_G

Cuts at:   2077    2435    2077 
   Size:        358    3448
 
BstAPI GCAn_nnn'nTGC

Cuts at:    145    1183     145 
   Size:       1038    2768
 
BstBI TT'CG_AA

Cuts at:   2167    2167 
   Size:       3806
 
BstYI r'GATC_y

Cuts at:      1     296     788     831    1298    1321    1656    1902    2914 
   Size:        295     492      43     467      23     335     246    1012

Cuts at:   2914    3000    3012    3358       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     492     467     449     346     335     295     246
                  86      43      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    314     314 
   Size:       3806
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1756    1937    2100    1756 
   Size:        181     163    3462

  Fragments arranged by size: 

                3462     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1049    1049 
   Size:       3806
 
DraI TTT'AAA

Cuts at:    805     805 
   Size:       3806
 
DrdI GACnn_nn'nnGTC

Cuts at:    768    1512    2370     768 
   Size:        744     858    2204

  Fragments arranged by size: 

                2204     858     744
 
EaeI y'GGCC_r

Cuts at:    570     705    1391    1565    1956    1983    3328     570 
   Size:        135     686     174     391      27    1345    1048

  Fragments arranged by size: 

                1345    1048     686     391     174     135      27
 
EagI C'GGCC_G

Cuts at:   1391    1391 
   Size:       3806
 
EarI CTCTTCn'nnn_

Cuts at:   1829    2039    1829 
   Size:        210    3596
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2014    2324    2470    3616    2014 
   Size:        310     146    1146    2204

  Fragments arranged by size: 

                2204    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    818     818 
   Size:       3806
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    154     698    1631    2063    2810    3078     154 
   Size:        544     933     432     747     268     882

  Fragments arranged by size: 

                 933     882     747     544     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    945     945 
   Size:       3806
 
EcoRI G'AATT_C

Cuts at:    810     810 
   Size:       3806
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1825    1825 
   Size:       3806
 
FspI TGC'GCA

Cuts at:   1587    1587 
   Size:       3806
 
HaeII r_GCGC'y

Cuts at:   1240    1488    2510    3489    1240 
   Size:        248    1022     979    1557

  Fragments arranged by size: 

                1557    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1648    1678    1680    1710    1648 
   Size:         30       2      30    3744

  Fragments arranged by size: 

                3744      30      30       2
 
HincII GTy'rAC

Cuts at:    845     845 
   Size:       3806
 
KasI G'GCGC_C

Cuts at:   1236    1484    1236 
   Size:        248    3558
 
KpnI G_GTAC'C

Cuts at:    826     826 
   Size:       3806
 
MluI A'CGCG_T

Cuts at:   3239    3239 
   Size:       3806
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    274    2477    2661     274 
   Size:       2203     184    1419

  Fragments arranged by size: 

                2203    1419     184
 
MscI TGG'CCA

Cuts at:   1567    1567 
   Size:       3806
 
MslI CAynn'nnrTG

Cuts at:   1795    1922    1795 
   Size:        127    3679
 
MspA1I CmG'CkG

Cuts at:    490     917    1231    1591    2604    2849     490 
   Size:        427     314     360    1013     245    1447

  Fragments arranged by size: 

                1447    1013     427     360     314     245
 
NaeI GCC'GGC

Cuts at:   1987    1987 
   Size:       3806
 
NarI GG'CG_CC

Cuts at:   1237    1485    1237 
   Size:        248    3558
 
NgoMIV G'CCGG_C

Cuts at:   1985    1985 
   Size:       3806
 
NsiI A_TGCA'T

Cuts at:    531     531 
   Size:       3806
 
NspI r_CATG'y

Cuts at:    190     392     859    1359    2266     190 
   Size:        202     467     500     907    1730

  Fragments arranged by size: 

                1730     907     500     467     202
 
PasI CC'CwG_GG

Cuts at:    540     540 
   Size:       3806
 
PciI A'CATG_T

Cuts at:    388    2262     388 
   Size:       1874    1932
 
PfoI T'CCnGG_A

Cuts at:    166     166 
   Size:       3806
 
PmeI GTTT'AAAC

Cuts at:    805     805 
   Size:       3806
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2945    2977    2945 
   Size:         32    3774
 
PstI C_TGCA'G

Cuts at:    180     559     853    1538     180 
   Size:        379     294     685    2448

  Fragments arranged by size: 

                2448     685     379     294
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3806
 
PvuII CAG'CTG

Cuts at:   1231    1591    1231 
   Size:        360    3446
 
RsrII CG'GwC_CG

Cuts at:   2001    2001 
   Size:       3806
 
SacI G_AGCT'C

Cuts at:    820     820 
   Size:       3806
 
SalI G'TCGA_C

Cuts at:    843     843 
   Size:       3806
 
SapI GCTCTTCn'nnn_

Cuts at:   1829    2039    1829 
   Size:        210    3596
 
SbfI CC_TGCA'GG

Cuts at:    853     853 
   Size:       3806
 
ScaI AGT'ACT

Cuts at:   3323    3323 
   Size:       3806
 
SfcI C'TryA_G

Cuts at:     33     176     555     849    1534    2527    2718      33 
   Size:        143     379     294     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     379     294     191     143
 
SfoI GGC'GCC

Cuts at:   1238    1486    1238 
   Size:        248    3558
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3806
 
SmaI CCC'GGG

Cuts at:    828     828 
   Size:       3806
 
SmlI C'TyrA_G

Cuts at:    961    1876    2368    2630     961 
   Size:        915     492     262    2137

  Fragments arranged by size: 

                2137     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3806
 
SphI G_CATG'C

Cuts at:    859    1359     859 
   Size:        500    3306
 
StyI C'CwwG_G

Cuts at:    337     940    1294     337 
   Size:        603     354    2849

  Fragments arranged by size: 

                2849     603     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1389    1522    1981    2190    1389 
   Size:        133     459     209    3005

  Fragments arranged by size: 

                3005     459     209     133
 
TatI w'GTAC_w

Cuts at:   3321    3321 
   Size:       3806
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3323    3355    3323 
   Size:         32    3774
 
Tth111I GACn'n_nGTC

Cuts at:   1603    1603 
   Size:       3806
 
XbaI T'CTAG_A

Cuts at:    837     837 
   Size:       3806
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1301    1301 
   Size:       3806
 
XmaI C'CCGG_G

Cuts at:    826     826 
   Size:       3806
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI   Bsu36I    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI
     NspI     PasI     PciI     PfoI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI     BaeI     BbsI    BbvCI     BglI    BmgBI     BmtI   Bpu10I
    BsaXI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     PacI    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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