Restriction map for pF1KB7298
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BclI        BfrI        BlnI        BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco47III    EcoRI       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
MfeI        MluI        MseI        MunI        NdeI        
NheI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SalI        ScaI        SfuI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        


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# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          127  GTCTAC               GT^[AC][GT]AC
AccIII       2460  TCCGGA               T^CCGGA
AcsI          490  AAATTT               [GA]^AATT[CT]
AocI         1995  CCTGAGG              CC^T[ACGT]AGG
ApaI         1697  GGGCCC               GGGCC^C
ApoI          490  AAATTT               [GA]^AATT[CT]
Asp700I      1356  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI        1554  CACGTG               CAC^GTG
BciVI        2847  GGATAC               GTATCC(6/5)
BfaI          826  CTAG                 C^TAG
BsaAI        1554  CACGTG               [CT]AC^GT[GA]
BsaBI         339  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2460  TCCGGA               [AT]^CCGG[AT]
BseAI        2460  TCCGGA               T^CCGGA
BspEI        2460  TCCGGA               T^CCGGA
BspHI           8  TCATGA               T^CATGA
BsrDI        1626  CATTGC               GCAATG(2/0)
BstEII        723  GGTCACC              G^GT[ACGT]ACC
BstPI         723  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1995  CCTGAGG              CC^T[ACGT]AGG
DraIII       1399  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1516  CGGCCG               C^GGCCG
EarI          817  CTCTTC               CTCTTC(1/4)
EclXI        1516  CGGCCG               C^GGCCG
Eco52I       1516  CGGCCG               C^GGCCG
Eco81I       1995  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       723  GGTCACC              G^GT[ACGT]ACC
HincII       1564  GTCAAC               GT[CT]^[GA]AC
HindII       1564  GTCAAC               GT[CT]^[GA]AC
Ksp632I       817  CTCTTC               CTCTTC(1/4)
MaeI          826  CTAG                 C^TAG
MamI          339  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2460  TCCGGA               T^CCGGA
NaeI         2642  GCCGGC               GCC^GGC
NcoI         2891  CCATGG               C^CATGG
NgoMIV       2642  GCCGGC               G^CCGGC
NotI         1515  GCGGCCGC             GC^GGCCGC
NruI          778  TCGCGA               TCG^CGA
PleI         2497  GACTC                GAGTC(4/5)
PmaCI        1554  CACGTG               CAC^GTG
PmlI         1554  CACGTG               CAC^GTG
PshAI        2464  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI            8  TCATGA               T^CATGA
SacI          151  GAGCTC               GAGCT^C
SapI          816  GCTCTTC              GCTCTTC(1/4)
SbfI         1767  CCTGCAGG             CCTGCA^GG
SexAI         919  ACCTGGT              A^CC[AT]GGT
SmaI         2042  CCCGGG               CCC^GGG
SphI         1532  GCATGC               GCATG^C
Sse8387I     1767  CCTGCAGG             CCTGCA^GG
XcmI         2594  CCAGCCCAGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2042  CCCGGG               C^CCGGG
XmaI         2042  CCCGGG               C^CCGGG
XmnI         1356  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

Alw44I        997  GTGCAC               G^TGCAC
             1501  GTGCAC              
AlwI         1650  GATCC                GGATC(4/5)
             2105  GATCC               
ApaLI         997  GTGCAC               G^TGCAC
             1501  GTGCAC              
BcgI          933  CGACCTTATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1635  GCACCTGAATCG        
BglII        1358  AGATCT               A^GATCT
             2657  AGATCT              
BsiEI         165  CGGTCG               CG[GA][CT]^CG
             1516  CGGCCG              
BsmBI         669  CGTCTC               CGTCTC(1/5)
             2665  CGTCTC              
KspI          438  CCGCGG               CCGC^GG
             1513  CCGCGG              
NspI         1439  GCATGT               [GA]CATG^[CT]
             1532  GCATGC              
SacII         438  CCGCGG               CCGC^GG
             1513  CCGCGG              
StuI         2542  AGGCCT               AGG^CCT
             2987  AGGCCT              
TfiI         1641  GAATC                G^A[AT]TC
             2531  GATTC               
Tsp509I       491  AATT                 ^AATT
             2603  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoNI       EcoO109I    EcoRII      EcoRV       
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfiI        SfoI        SmlI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       
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