Restriction map for pF1KB7298
 (Linear) (Six-base) MAPSORT of: KIBB7298.seq  Check: 1507  from: 1  to: 3051



KIBB7298.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0     128    3051 
   Size:        128    2923
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    2882    3051 
   Size:       1027    1855     169

  Fragments arranged by size: 

                1855    1027     169
 
AflIII A'CryG_T

Cuts at:      0     587    1331    3041    3051 
   Size:        587     744    1710      10

  Fragments arranged by size: 

                1710     744     587      10
 
AleI CACnn'nnGTG

Cuts at:      0     856    1315    3051 
   Size:        856     459    1736

  Fragments arranged by size: 

                1736     856     459
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     103    3051 
   Size:        103    2948
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246    1050    1688    1968    2905    2956    3051 
   Size:        246     804     638     280     937      51      95

  Fragments arranged by size: 

                 937     804     638     280     246      95      51
 
ApaI G_GGCC'C

Cuts at:      0    1701    3051 
   Size:       1701    1350
 
ApaLI G'TGCA_C

Cuts at:      0     997    1501    3051 
   Size:        997     504    1550

  Fragments arranged by size: 

                1550     997     504
 
ApoI r'AATT_y

Cuts at:      0     490    3051 
   Size:        490    2561
 
AvaI C'yCGr_G

Cuts at:      0     613     750    2042    2261    3051 
   Size:        613     137    1292     219     790

  Fragments arranged by size: 

                1292     790     613     219     137
 
BanI G'GyrC_C

Cuts at:      0     323    1151    1227    1387    2571    3051 
   Size:        323     828      76     160    1184     480

  Fragments arranged by size: 

                1184     828     480     323     160      76
 
BanII G_rGCy'C

Cuts at:      0     155    1701    2074    2269    3051 
   Size:        155    1546     373     195     782

  Fragments arranged by size: 

                1546     782     373     195     155
 
BbeI G_GCGC'C

Cuts at:      0    1155    1391    2575    3051 
   Size:       1155     236    1184     476

  Fragments arranged by size: 

                1184    1155     476     236
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     193     513    1015    3051 
   Size:        193     320     502    2036

  Fragments arranged by size: 

                2036     502     320     193
 
BbvCI CC'TCA_GC

Cuts at:      0    1988    2089    2245    2423    2513    2618    2794    3051 
   Size:       1988     101     156     178      90     105     176     257

  Fragments arranged by size: 

                1988     257     178     176     156     105     101      90
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     922     956    1624    1658    3051 
   Size:        922      34     668      34    1393

  Fragments arranged by size: 

                1393     922     668      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2841    3051 
   Size:       2841     210
 
BglI GCCn_nnn'nGGC

Cuts at:      0     548     557     566    1771    2029    2223    2285    2614 
   Size:        548       9       9    1205     258     194      62     329

Cuts at:   2614    3051 
   Size:        437

  Fragments arranged by size: 

                1205     548     437     329     258     194      62       9
                   9
 
BglII A'GATC_T

Cuts at:      0    1358    2657    3051 
   Size:       1358    1299     394

  Fragments arranged by size: 

                1358    1299     394
 
BlpI GC'TnA_GC

Cuts at:      0     312    1969    2689    3051 
   Size:        312    1657     720     362

  Fragments arranged by size: 

                1657     720     362     312
 
Bme1580I G_kGCm'C

Cuts at:      0     109     614    1001    1232    1505    1701    3051 
   Size:        109     505     387     231     273     196    1350

  Fragments arranged by size: 

                1350     505     387     273     231     196     109
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     858    2452    3051 
   Size:        858    1594     599

  Fragments arranged by size: 

                1594     858     599
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1077    3051 
   Size:       1077    1974
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280    1072    1243    2827    2960    3051 
   Size:        280     792     171    1584     133      91

  Fragments arranged by size: 

                1584     792     280     171     133      91
 
Bpu10I CC'TnA_GC

Cuts at:      0    1871    1988    2089    2245    2423    2513    2618    2794 
   Size:       1871     117     101     156     178      90     105     176

Cuts at:   2794    3051 
   Size:        257

  Fragments arranged by size: 

                1871     257     178     176     156     117     105     101
                  90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1468    1507    1822    2940    3033    3051 
   Size:       1468      39     315    1118      93      18

  Fragments arranged by size: 

                1468    1118     315      93      39      18
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     214     647    2916    3051 
   Size:        214     433    2269     135

  Fragments arranged by size: 

                2269     433     214     135
 
BsaAI yAC'GTr

Cuts at:      0    1556    3051 
   Size:       1556    1495
 
BsaBI GATnn'nnATC

Cuts at:      0     343    3051 
   Size:        343    2708
 
BsaHI Gr'CG_yC

Cuts at:      0    1152    1388    1580    2572    3051 
   Size:       1152     236     192     992     479

  Fragments arranged by size: 

                1152     992     479     236     192
 
BsaWI w'CCGG_w

Cuts at:      0    2460    3051 
   Size:       2460     591
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475     655     685    1876    1906    2213    2243 
   Size:        445      30     180      30    1191      30     307      30

Cuts at:   2243    2362    2392    2825    2855    3051 
   Size:        119      30     433      30     196

  Fragments arranged by size: 

                1191     445     433     307     196     180     119      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     643     646     878    2136    2139    2305    2310    2635 
   Size:        643       3     232    1258       3     166       5     325

Cuts at:   2635    3051 
   Size:        416

  Fragments arranged by size: 

                1258     643     416     325     232     166       5       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0    2120    2250    2593    2733    2751    2836    2874    3051 
   Size:       2120     130     343     140      18      85      38     177

  Fragments arranged by size: 

                2120     343     177     140     130      85      38      18
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232    1089    1356    1693    3051 
   Size:        232     857     267     337    1358

  Fragments arranged by size: 

                1358     857     337     267     232
 
BsiEI CG_ry'CG

Cuts at:      0     168    1519    3051 
   Size:        168    1351    1532

  Fragments arranged by size: 

                1532    1351     168
 
BsiHKAI G_wGCw'C

Cuts at:      0     155     278    1001    1085    1505    1811    2404    3051 
   Size:        155     123     723      84     420     306     593     647

  Fragments arranged by size: 

                 723     647     593     420     306     155     123      84
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     675    2671    3051 
   Size:        675    1996     380

  Fragments arranged by size: 

                1996     675     380
 
Bsp1286I G_dGCh'C

Cuts at:      0     109     155     278     614    1001    1085    1232    1505 
   Size:        109      46     123     336     387      84     147     273

Cuts at:   1505    1701    1811    2074    2269    2404    3051 
   Size:        196     110     263     195     135     647

  Fragments arranged by size: 

                 647     387     336     273     263     196     195     147
                 135     123     110     109      84      46
 
BspEI T'CCGG_A

Cuts at:      0    2460    3051 
   Size:       2460     591
 
BspHI T'CATG_A

Cuts at:      0       8    3051 
   Size:          8    3043
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     199     968    2069    3051 
   Size:        199     769    1101     982

  Fragments arranged by size: 

                1101     982     769     199
 
BsrBI CCG'CTC

Cuts at:      0    1056    1454    1521    2129    2564    3051 
   Size:       1056     398      67     608     435     487

  Fragments arranged by size: 

                1056     608     487     435     398      67
 
BsrDI GCAATG_nn'

Cuts at:      0    1625    3051 
   Size:       1625    1426
 
BsrFI r'CCGG_y

Cuts at:      0       3     538    2642    3051 
   Size:          3     535    2104     409

  Fragments arranged by size: 

                2104     535     409       3
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     246    1509    1533    1688    3051 
   Size:        246    1263      24     155    1363

  Fragments arranged by size: 

                1363    1263     246     155      24
 
BstEII G'GTnAC_C

Cuts at:      0     723    3051 
   Size:        723    2328
 
BstYI r'GATC_y

Cuts at:      0    1358    1649    2657    3051 
   Size:       1358     291    1008     394

  Fragments arranged by size: 

                1358    1008     394     291
 
Bsu36I CC'TnA_GG

Cuts at:      0    1996    3051 
   Size:       1996    1055
 
BtgI C'CryG_G

Cuts at:      0     438     632     806    1513    1755    2891    3051 
   Size:        438     194     174     707     242    1136     160

  Fragments arranged by size: 

                1136     707     438     242     194     174     160
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      36     786    1263    3051 
   Size:         36     750     477    1788

  Fragments arranged by size: 

                1788     750     477      36
 
BtsI GCAGTG_nn'

Cuts at:      0    1077    2868    3051 
   Size:       1077    1791     183

  Fragments arranged by size: 

                1791    1077     183
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      45      80    3051 
   Size:         45      35    2971

  Fragments arranged by size: 

                2971      45      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1404    3051 
   Size:       1404    1647
 
EaeI y'GGCC_r

Cuts at:      0      92     540     549     870     914    1516    1829    2361 
   Size:         92     448       9     321      44     602     313     532

Cuts at:   2361    2606    3051 
   Size:        245     445

  Fragments arranged by size: 

                 602     532     448     445     321     313     245      92
                  44       9
 
EagI C'GGCC_G

Cuts at:      0    1516    3051 
   Size:       1516    1535
 
EarI CTCTTCn'nnn_

Cuts at:      0     823    3051 
   Size:        823    2228
 
EcoICRI GAG'CTC

Cuts at:      0     153    3051 
   Size:        153    2898
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280    1027    1072    1243    2827    2882    2960    3051 
   Size:        280     747      45     171    1584      55      78      91

  Fragments arranged by size: 

                1584     747     280     171      91      78      55      45
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1840    2065    2722    3051 
   Size:       1840     225     657     329

  Fragments arranged by size: 

                1840     657     329     225
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1479    1698    1848    1992    2046    2216    2517    2558 
   Size:       1479     219     150     144      54     170     301      41

Cuts at:   2558    2737    2968    3051 
   Size:        179     231      83

  Fragments arranged by size: 

                1479     301     231     219     179     170     150     144
                  83      54      41
 
EcoRV GAT'ATC

Cuts at:      0     396     831    1866    3051 
   Size:        396     435    1035    1185

  Fragments arranged by size: 

                1185    1035     435     396
 
HaeII r_GCGC'y

Cuts at:      0    1155    1274    1391    2575    3051 
   Size:       1155     119     117    1184     476

  Fragments arranged by size: 

                1184    1155     476     119     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1850    1882    1989    2021    2213    2245    2649    2681 
   Size:       1850      32     107      32     192      32     404      32

Cuts at:   2681    3051 
   Size:        370

  Fragments arranged by size: 

                1850     404     370     192     107      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1566    3051 
   Size:       1566    1485
 
KasI G'GCGC_C

Cuts at:      0    1151    1387    2571    3051 
   Size:       1151     236    1184     480

  Fragments arranged by size: 

                1184    1151     480     236
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     161    3051 
   Size:        161    2890
 
MscI TGG'CCA

Cuts at:      0      94     916    1831    2363    2608    3051 
   Size:         94     822     915     532     245     443

  Fragments arranged by size: 

                 915     822     532     443     245      94
 
MslI CAynn'nnrTG

Cuts at:      0     805     856     982    1315    1498    1630    1781    3051 
   Size:        805      51     126     333     183     132     151    1270

  Fragments arranged by size: 

                1270     805     333     183     151     132     126      51
            
 
MspA1I CmG'CkG

Cuts at:      0     197     440    1179    1515    1571    1659    1968    2512 
   Size:        197     243     739     336      56      88     309     544

Cuts at:   2512    2793    3051 
   Size:        281     258

  Fragments arranged by size: 

                 739     544     336     309     281     258     243     197
                  88      56
 
NaeI GCC'GGC

Cuts at:      0    2644    3051 
   Size:       2644     407
 
NarI GG'CG_CC

Cuts at:      0    1152    1388    2572    3051 
   Size:       1152     236    1184     479

  Fragments arranged by size: 

                1184    1152     479     236
 
NcoI C'CATG_G

Cuts at:      0    2891    3051 
   Size:       2891     160
 
NgoMIV G'CCGG_C

Cuts at:      0    2642    3051 
   Size:       2642     409
 
NotI GC'GGCC_GC

Cuts at:      0    1516    3051 
   Size:       1516    1535
 
NruI TCG'CGA

Cuts at:      0     780    3051 
   Size:        780    2271
 
NspI r_CATG'y

Cuts at:      0    1443    1536    3051 
   Size:       1443      93    1515

  Fragments arranged by size: 

                1515    1443      93
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     421     912    1125    3051 
   Size:        421     491     213    1926

  Fragments arranged by size: 

                1926     491     421     213
 
PfoI T'CCnGG_A

Cuts at:      0      41     329    2843    2912    3051 
   Size:         41     288    2514      69     139

  Fragments arranged by size: 

                2514     288     139      69      41
 
PmlI CAC'GTG

Cuts at:      0    1556    3051 
   Size:       1556    1495
 
PpuMI rG'GwC_Cy

Cuts at:      0    1479    1848    1992    2046    2737    2968    3051 
   Size:       1479     369     144      54     691     231      83

  Fragments arranged by size: 

                1479     691     369     231     144      83      54
 
PshAI GACnn'nnGTC

Cuts at:      0    2468    3051 
   Size:       2468     583
 
PspOMI G'GGCC_C

Cuts at:      0    1697    3051 
   Size:       1697    1354
 
PstI C_TGCA'G

Cuts at:      0     196     242    1046    1658    1772    1964    2726    3051 
   Size:        196      46     804     612     114     192     762     325

  Fragments arranged by size: 

                 804     762     612     325     196     192     114      46
            
 
PvuII CAG'CTG

Cuts at:      0     197    1659    1968    2512    2793    3051 
   Size:        197    1462     309     544     281     258

  Fragments arranged by size: 

                1462     544     309     281     258     197
 
SacI G_AGCT'C

Cuts at:      0     155    3051 
   Size:        155    2896
 
SacII CC_GC'GG

Cuts at:      0     441    1516    3051 
   Size:        441    1075    1535

  Fragments arranged by size: 

                1535    1075     441
 
SanDI GG'GwC_CC

Cuts at:      0    2737    2968    3051 
   Size:       2737     231      83

  Fragments arranged by size: 

                2737     231      83
 
SapI GCTCTTCn'nnn_

Cuts at:      0     823    3051 
   Size:        823    2228
 
SbfI CC_TGCA'GG

Cuts at:      0    1772    3051 
   Size:       1772    1279
 
SexAI A'CCwGG_T

Cuts at:      0     919    3051 
   Size:        919    2132
 
SfcI C'TryA_G

Cuts at:      0     192     238     663    1042    1654    1768    1960    2579 
   Size:        192      46     425     379     612     114     192     619

Cuts at:   2579    2722    3051 
   Size:        143     329

  Fragments arranged by size: 

                 619     612     425     379     329     192     192     143
                 114      46
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     548     557    2223    3051 
   Size:        548       9    1666     828

  Fragments arranged by size: 

                1666     828     548       9
 
SfoI GGC'GCC

Cuts at:      0    1153    1389    2573    3051 
   Size:       1153     236    1184     478

  Fragments arranged by size: 

                1184    1153     478     236
 
SmaI CCC'GGG

Cuts at:      0    2044    3051 
   Size:       2044    1007
 
SmlI C'TyrA_G

Cuts at:      0    1483    1522    1801    2919    3012    3051 
   Size:       1483      39     279    1118      93      39

  Fragments arranged by size: 

                1483    1118     279      93      39      39
 
SphI G_CATG'C

Cuts at:      0    1536    3051 
   Size:       1536    1515
 
StuI AGG'CCT

Cuts at:      0    2544    2989    3051 
   Size:       2544     445      62

  Fragments arranged by size: 

                2544     445      62
 
StyI C'CwwG_G

Cuts at:      0     429     465     674    2554    2891    3051 
   Size:        429      36     209    1880     337     160

  Fragments arranged by size: 

                1880     429     337     209     160      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     485    1089    2765    2850    3051 
   Size:        485     604    1676      85     201

  Fragments arranged by size: 

                1676     604     485     201      85
 
TatI w'GTAC_w

Cuts at:      0     617    1289    1442    3051 
   Size:        617     672     153    1609

  Fragments arranged by size: 

                1609     672     617     153
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     445     477    2362    2394    2825    2857    3051 
   Size:        445      32    1885      32     431      32     194

  Fragments arranged by size: 

                1885     445     431     194      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2601    3051 
   Size:       2601     450
 
XmaI C'CCGG_G

Cuts at:      0    2042    3051 
   Size:       2042    1009
 
XmnI GAAnn'nnTTC

Cuts at:      0    1360    3051 
   Size:       1360    1691
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NotI     NruI     NspI    PflMI     PfoI     PmlI
    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BclI
    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsrGI   BssHII    BssSI
    BstBI    BstXI  BstZ17I     ClaI     DraI     DrdI     EciI    EcoRI
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NsiI     PacI     PasI     PciI     PmeI
     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     ScaI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I
     XbaI     XhoI     ZraI
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