Restriction map for pF1KB7296
 (Circular) (Six-base) MAPSORT of: pF1KB7296.seq  Check: 9787  from: 1  to: 7112



pF1KB7296  7112 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:   4150    4150 
   Size:       7112
 
Acc65I G'GTAC_C

Cuts at:   3250    4128    3250 
   Size:        878    6234
 
AclI AA'CG_TT

Cuts at:   6952    6952 
   Size:       7112
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    226     293    1721    1874    4937    5369    5722    6384     226 
   Size:         67    1428     153    3063     432     353     662     954

  Fragments arranged by size: 

                3063    1428     954     662     432     353     153      67
            
 
AflIII A'CryG_T

Cuts at:    324    1459    1461    4464    6264    6545     324 
   Size:       1135       2    3003    1800     281     891

  Fragments arranged by size: 

                3003    1800    1135     891     281       2
 
AlwNI CAG_nnn'CTG

Cuts at:    123    1846    1921    2065    2080    3340    3589    4417    5855 
   Size:       1723      75     144      15    1260     249     828    1438

Cuts at:   5855     123 
   Size:       1380

  Fragments arranged by size: 

                1723    1438    1380    1260     828     249     144      75
                  15
 
ApaLI G'TGCA_C

Cuts at:   5950    5950 
   Size:       7112
 
ApoI r'AATT_y

Cuts at:    434     740    1435    1898    2054    2254    2637    2742    2804 
   Size:        306     695     463     156     200     383     105      62

Cuts at:   2804    2900    3118    3348    4116    6465    6721     434 
   Size:         96     218     230     768    2349     256     825

  Fragments arranged by size: 

                2349     825     768     695     463     383     306     256
                 230     218     200     156     105      96      62
 
AseI AT'TA_AT

Cuts at:     20     259     656    1610    2873      20 
   Size:        239     397     954    1263    4259

  Fragments arranged by size: 

                4259    1263     954     397     239
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7112
 
AvaI C'yCGr_G

Cuts at:    220    1004    4132     220 
   Size:        784    3128    3200

  Fragments arranged by size: 

                3200    3128     784
 
AvrII C'CTAG_G

Cuts at:    459    3658     459 
   Size:       3199    3913
 
BamHI G'GATC_C

Cuts at:   3308    4137    3308 
   Size:        829    6283
 
BanI G'GyrC_C

Cuts at:    177    3250    4128    4542    4790    4825     177 
   Size:       3073     878     414     248      35    2464

  Fragments arranged by size: 

                3073    2464     878     414     248      35
 
BanII G_rGCy'C

Cuts at:    794    3442    4126    5156     794 
   Size:       2648     684    1030    2750

  Fragments arranged by size: 

                2750    2648    1030     684
 
BbeI G_GCGC'C

Cuts at:   4546    4794    4546 
   Size:        248    6864
 
BbsI GAAGACnn'nnnn_

Cuts at:    965    1217    1308    2549    3582     965 
   Size:        252      91    1241    1033    4495

  Fragments arranged by size: 

                4495    1241    1033     252      91
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    7078
 
BciVI GTATCCnnnnn_n'

Cuts at:   5010    6066    7112    5010 
   Size:       1056    1046    5010

  Fragments arranged by size: 

                5010    1056    1046
 
BclI T'GATC_A

Cuts at:   4632    4632 
   Size:       7112
 
BfrBI ATG'CAT

Cuts at:   2230    2230 
   Size:       7112
 
BglI GCCn_nnn'nGGC

Cuts at:    112     162     112 
   Size:         50    7062
 
BglII A'GATC_T

Cuts at:   2487    3499    4627    6664    2487 
   Size:       1012    1128    2037    2935

  Fragments arranged by size: 

                2935    2037    1128    1012
 
BlpI GC'TnA_GC

Cuts at:    293    4224     293 
   Size:       3931    3181
 
Bme1580I G_kGCm'C

Cuts at:   2417    4737    4830    5954    2417 
   Size:       2320      93    1124    3575

  Fragments arranged by size: 

                3575    2320    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    418     895    2202    4324    4405    4740    6884     418 
   Size:        477    1307    2122      81     335    2144     646

  Fragments arranged by size: 

                2144    2122    1307     646     477     335      81
 
BmtI G_CTAG'C

Cuts at:   1559    1559 
   Size:       7112
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1866    2396    5479    1866 
   Size:        530    3083    3499

  Fragments arranged by size: 

                3499    3083     530
 
Bpu10I CC'TnA_GC

Cuts at:   2066    3170    2066 
   Size:       1104    6008
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2003    2136    2743    3435    3771    4288    5167    5881    6179 
   Size:        133     607     692     336     517     879     714     298

Cuts at:   6179    2003 
   Size:       2936

  Fragments arranged by size: 

                2936     879     714     692     607     517     336     298
                 133
 
BsaI GGTCTCn'nnnn_

Cuts at:   6816    6816 
   Size:       7112
 
BsaAI yAC'GTr

Cuts at:   1462    1949    4465    1462 
   Size:        487    2516    4109

  Fragments arranged by size: 

                4109    2516     487
 
BsaBI GATnn'nnATC

Cuts at:      6    2231    3428    4626    6663       6 
   Size:       2225    1197    1198    2037     455

  Fragments arranged by size: 

                2225    2037    1198    1197     455
 
BsaHI Gr'CG_yC

Cuts at:   4543    4791    5493    6989    4543 
   Size:        248     702    1496    4666

  Fragments arranged by size: 

                4666    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1745    3707    4301    4822    5500    5911    6058    6332    1745 
   Size:       1962     594     521     678     411     147     274    2525

  Fragments arranged by size: 

                2525    1962     678     594     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   3788    3818    3867    3885    3897    3915    3788 
   Size:         30      49      18      12      18    6985

  Fragments arranged by size: 

                6985      49      30      18      18      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1813    3548    3875    3878    3893    1813 
   Size:       1735     327       3      15    5032

  Fragments arranged by size: 

                5032    1735     327      15       3
 
BseYI C'CCAG_C

Cuts at:   3594    3645    3970    4537    5295    5960    6368    3594 
   Size:         51     325     567     758     665     408    4338

  Fragments arranged by size: 

                4338     758     665     567     408     325      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2141    3326    2141 
   Size:       1185    5927
 
BsiEI CG_ry'CG

Cuts at:    104    4654    4700    5930     104 
   Size:       4550      46    1230    1286

  Fragments arranged by size: 

                4550    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   2204    4049    4126    4904    5094    5954    2204 
   Size:       1845      77     778     190     860    3362

  Fragments arranged by size: 

                3362    1845     860     778     190      77
 
BsmI GAATG_Cn'

Cuts at:   4098    4098 
   Size:       7112
 
Bsp1286I G_dGCh'C

Cuts at:    794    2204    2417    3442    4049    4126    4737    4830    4904 
   Size:       1410     213    1025     607      77     611      93      74

Cuts at:   4904    5094    5156    5954     794 
   Size:        190      62     798    1952

  Fragments arranged by size: 

                1952    1410    1025     798     611     607     213     190
                  93      77      74      62
 
BspHI T'CATG_A

Cuts at:   1029    2820    5544    1029 
   Size:       1791    2724    2597

  Fragments arranged by size: 

                2724    2597    1791
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2441    4162    4376    4678    5059    2441 
   Size:       1721     214     302     381    4494

  Fragments arranged by size: 

                4494    1721     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    5404    5458    6942    7107      50 
   Size:       5354      54    1484     165      55

  Fragments arranged by size: 

                5354    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    608    1566    2288    3462    3849    3965    5024     608 
   Size:        958     722    1174     387     116    1059    2696

  Fragments arranged by size: 

                2696    1174    1059     958     722     387     116
 
BsrFI r'CCGG_y

Cuts at:   5110    5291    5110 
   Size:        181    6931
 
BsrGI T'GTAC_A

Cuts at:   2502    2502 
   Size:       7112
 
BssSI C'ACGA_G

Cuts at:   3755    5383    6091    3755 
   Size:       1628     708    4776

  Fragments arranged by size: 

                4776    1628     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    123     607    4489     123 
   Size:        484    3882    2746

  Fragments arranged by size: 

                3882    2746     484
 
BstBI TT'CG_AA

Cuts at:   5473    5473 
   Size:       7112
 
BstYI r'GATC_y

Cuts at:      1    1965    2002    2487    3017    3308    3499    4137    4604 
   Size:       1964      37     485     530     291     191     638     467

Cuts at:   4604    4627    4962    5208    5514    5526    5612    6664       1 
   Size:         23     335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1964    1052     638     530     485     467     449     335
                 306     291     246     191      86      37      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:    693    2942     693 
   Size:       2249    4863
 
BtgI C'CryG_G

Cuts at:   3489    3837    3489 
   Size:        348    6764
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   5062    5243    5406    5062 
   Size:        181     163    6768

  Fragments arranged by size: 

                6768     181     163
 
BtsI GCAGTG_nn'

Cuts at:   3420    4355    3420 
   Size:        935    6177
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   4067    4102    4067 
   Size:         35    7077
 
DraI TTT'AAA

Cuts at:    533    1226    1661    4111     533 
   Size:        693     435    2450    3534

  Fragments arranged by size: 

                3534    2450     693     435
 
DraIII CAC_nnn'GTG

Cuts at:   2606    2884    2606 
   Size:        278    6834
 
DrdI GACnn_nn'nnGTC

Cuts at:   4818    6162    4818 
   Size:       1344    5768
 
EaeI y'GGCC_r

Cuts at:   4697    4871    5262    5289    6634    4697 
   Size:        174     391      27    1345    5175

  Fragments arranged by size: 

                5175    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   4697    4697 
   Size:       7112
 
EarI CTCTTCn'nnn_

Cuts at:    140     245     674    1697    1784    1850    1868    5135    5345 
   Size:        105     429    1023      87      66      18    3267     210

Cuts at:   5345     140 
   Size:       1907

  Fragments arranged by size: 

                3267    1907    1023     429     210     105      87      66
                  18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3365    5320    6062    6208    6922    3365 
   Size:       1955     742     146     714    3555

  Fragments arranged by size: 

                3555    1955     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   4124    4124 
   Size:       7112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    226     293    1721    1874    4937    5369    5722    6384     226 
   Size:         67    1428     153    3063     432     353     662     954

  Fragments arranged by size: 

                3063    1428     954     662     432     353     153      67
            
 
EcoO109I rG'GnC_Cy

Cuts at:    456    2946    4251     456 
   Size:       2490    1305    3317

  Fragments arranged by size: 

                3317    2490    1305
 
EcoRI G'AATT_C

Cuts at:   1898    2742    4116    1898 
   Size:        844    1374    4894

  Fragments arranged by size: 

                4894    1374     844
 
EcoRV GAT'ATC

Cuts at:   1733    3066    1733 
   Size:       1333    5779
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    428    1471    3521    3973    5131     428 
   Size:       1043    2050     452    1158    2409

  Fragments arranged by size: 

                2409    2050    1158    1043     452
 
FspI TGC'GCA

Cuts at:   4893    4893 
   Size:       7112
 
HaeII r_GCGC'y

Cuts at:   4546    4794    6024    6795    4546 
   Size:        248    1230     771    4863

  Fragments arranged by size: 

                4863    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    364     396    2820    2852    3697    3729    3769    3801    4954 
   Size:         32    2424      32     845      32      40      32    1153

Cuts at:   4954    4984    4986    5016     364 
   Size:         30       2      30    2460

  Fragments arranged by size: 

                2460    2424    1153     845      40      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   3368    4151    3368 
   Size:        783    6329
 
HpaI GTT'AAC

Cuts at:   3368    3368 
   Size:       7112
 
KasI G'GCGC_C

Cuts at:   4542    4790    4542 
   Size:        248    6864
 
KpnI G_GTAC'C

Cuts at:   3254    4132    3254 
   Size:        878    6234
 
MfeI C'AATT_G

Cuts at:    350    1713     350 
   Size:       1363    5749
 
MluI A'CGCG_T

Cuts at:   6545    6545 
   Size:       7112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2502    3831    4067    5871    6055    2502 
   Size:       1329     236    1804     184    3559

  Fragments arranged by size: 

                3559    1804    1329     236     184
 
MscI TGG'CCA

Cuts at:   4873    4873 
   Size:       7112
 
MslI CAynn'nnrTG

Cuts at:    641    2210    3842    4070    5101    5228     641 
   Size:       1569    1632     228    1031     127    2525

  Fragments arranged by size: 

                2525    1632    1569    1031     228     127
 
MspA1I CmG'CkG

Cuts at:    123    1699    4223    4537    4897    5681    5926     123 
   Size:       1576    2524     314     360     784     245    1309

  Fragments arranged by size: 

                2524    1576    1309     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   5293    5293 
   Size:       7112
 
NarI GG'CG_CC

Cuts at:   4543    4791    4543 
   Size:        248    6864
 
NcoI C'CATG_G

Cuts at:   3489    3837    3489 
   Size:        348    6764
 
NdeI CA'TA_TG

Cuts at:    990    1955     990 
   Size:        965    6147
 
NgoMIV G'CCGG_C

Cuts at:   5291    5291 
   Size:       7112
 
NheI G'CTAG_C

Cuts at:   1555    1555 
   Size:       7112
 
NsiI A_TGCA'T

Cuts at:   2232    2232 
   Size:       7112
 
NspI r_CATG'y

Cuts at:   1322    1645    1884    4165    4665    6268    1322 
   Size:        323     239    2281     500    1603    2166

  Fragments arranged by size: 

                2281    2166    1603     500     323     239
 
PciI A'CATG_T

Cuts at:   6264    6264 
   Size:       7112
 
PmeI GTTT'AAAC

Cuts at:   4111    4111 
   Size:       7112
 
PmlI CAC'GTG

Cuts at:   1462    1462 
   Size:       7112
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5558    5590    5558 
   Size:         32    7080
 
PpuMI rG'GwC_Cy

Cuts at:    456    2946     456 
   Size:       2490    4622
 
PsiI TTA'TAA

Cuts at:    467     775    1441    2038    2974     467 
   Size:        308     666     597     936    4605

  Fragments arranged by size: 

                4605     936     666     597     308
 
PstI C_TGCA'G

Cuts at:    525    3278    4159    4844     525 
   Size:       2753     881     685    2793

  Fragments arranged by size: 

                2793    2753     881     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7112
 
PvuII CAG'CTG

Cuts at:    123    1699    4537    4897     123 
   Size:       1576    2838     360    2338

  Fragments arranged by size: 

                2838    2338    1576     360
 
RsrII CG'GwC_CG

Cuts at:   5307    5307 
   Size:       7112
 
SacI G_AGCT'C

Cuts at:   4126    4126 
   Size:       7112
 
SalI G'TCGA_C

Cuts at:   4149    4149 
   Size:       7112
 
SapI GCTCTTCn'nnn_

Cuts at:    245     674    5135    5345     245 
   Size:        429    4461     210    2012

  Fragments arranged by size: 

                4461    2012     429     210
 
SbfI CC_TGCA'GG

Cuts at:   4159    4159 
   Size:       7112
 
ScaI AGT'ACT

Cuts at:   6629    6629 
   Size:       7112
 
SexAI A'CCwGG_T

Cuts at:    479    1020    2349    3246     479 
   Size:        541    1329     897    4345

  Fragments arranged by size: 

                4345    1329     897     541
 
SfcI C'TryA_G

Cuts at:     33     521    1783    2093    3274    4155    4840    5808    5999 
   Size:        488    1262     310    1181     881     685     968     191

Cuts at:   5999      33 
   Size:       1146

  Fragments arranged by size: 

                1262    1181    1146     968     881     685     488     310
                 191
 
SfoI GGC'GCC

Cuts at:   4544    4792    4544 
   Size:        248    6864
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7112
 
SmaI CCC'GGG

Cuts at:   4134    4134 
   Size:       7112
 
SmlI C'TyrA_G

Cuts at:    220    1004    2018    2115    2722    3450    3786    4267    5182 
   Size:        784    1014      97     607     728     336     481     915

Cuts at:   5182    5896    6158     220 
   Size:        714     262    1174

  Fragments arranged by size: 

                1174    1014     915     784     728     714     607     481
                 336     262      97
 
SnaBI TAC'GTA

Cuts at:   1949    1949 
   Size:       7112
 
SpeI A'CTAG_T

Cuts at:     67     939      67 
   Size:        872    6240
 
SphI G_CATG'C

Cuts at:   1645    4165    4665    1645 
   Size:       2520     500    4092

  Fragments arranged by size: 

                4092    2520     500
 
SspI AAT'ATT

Cuts at:   1392    1863    2361    2851    1392 
   Size:        471     498     490    5653

  Fragments arranged by size: 

                5653     498     490     471
 
StyI C'CwwG_G

Cuts at:    459    1889    3286    3489    3658    3837    4246    4600     459 
   Size:       1430    1397     203     169     179     409     354    2971

  Fragments arranged by size: 

                2971    1430    1397     409     354     203     179     169
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2462    4070    4695    4828    5287    5496    2462 
   Size:       1608     625     133     459     209    4078

  Fragments arranged by size: 

                4078    1608     625     459     209     133
 
TatI w'GTAC_w

Cuts at:   1750    2502    3331    6627    1750 
   Size:        752     829    3296    2235

  Fragments arranged by size: 

                3296    2235     829     752
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    161     193    3788    3820    6629    6661     161 
   Size:         32    3595      32    2809      32     612

  Fragments arranged by size: 

                3595    2809     612      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2351    4909    2351 
   Size:       2558    4554
 
XbaI T'CTAG_A

Cuts at:   4143    4143 
   Size:       7112
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    356    1134    4607     356 
   Size:        778    3473    2861

  Fragments arranged by size: 

                3473    2861     778
 
XhoI C'TCGA_G

Cuts at:    220    1004     220 
   Size:        784    6328
 
XmaI C'CCGG_G

Cuts at:   4132    4132 
   Size:       7112
 
XmnI GAAnn'nnTTC

Cuts at:    306     326     434    1109     306 
   Size:         20     108     675    6309

  Fragments arranged by size: 

                6309     675     108      20
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NsiI     NspI     PciI     PmeI     PmlI     PpiI
    PpuMI     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI     BaeI    BbvCI    BmgBI     BpmI    BsiWI
    BsmBI    BspEI   BssHII   BstEII    BstXI  BstZ17I     ClaI    EcoNI
     FseI    FspAI  HindIII     NotI     NruI     PacI     PasI    PflMI
     PfoI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI     SrfI     StuI     SwaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com