Restriction map for pF1KB7289
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BclI        
BfrI        BglII       BlnI        BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BsrGI       BssSI       
Bst1107I    BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoRI       EcoT22I     FbaI        FspI        
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MunI        NarI        NcoI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PvuI        RsrII       
SacII       SalI        SexAI       SfiI        SfoI        
SgrAI       SmlI        SnaBI       SphI        SspBI       
SspI        SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AclI         1363  AACGTT               AA^CGTT
AcsI          742  AAATTC               [GA]^AATT[CT]
AcyI         2575  GGCGTC               G[GA]^CG[CT]C
AflIII       2387  ACATGT               A^C[GA][CT]GT
AocI         2728  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1590  AGCGCT               AGC^GCT
ApoI          742  AAATTC               [GA]^AATT[CT]
AscI         2095  GGCGCGCC             GG^CGCGCC
AspHI        2333  GAGCTC               G[AT]GC[AT]^C
AspI          406  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         443  GGTCC                G^G[AT]CC
BsaHI        2575  GGCGTC               G[GA]^CG[CT]C
BsaI         2782  GAGACC               GGTCTC(1/5)
BsiEI        1960  CGGTCG               CG[GA][CT]^CG
BsiHKAI      2333  GAGCTC               G[AT]GC[AT]^C
BspHI        1394  TCATGA               T^CATGA
BspLU11I     2387  ACATGT               A^CATGT
BsrBI        1957  GAGCGG               CCGCTC(-3/-3)
BsrFI        2108  GCCGGC               [GA]^CCGG[CT]
BstAPI       1164  GCACCGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1639  TTCGAA               TT^CGAA
Bsu36I       2728  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       2108  GCCGGC               [GA]^CCGG[CT]
Eco47III     1590  AGCGCT               AGC^GCT
Eco81I       2728  CCTGAGG              CC^T[ACGT]AGG
EcoRV         793  GATATC               GAT^ATC
FseI         2107  GGCCGGCC             GGCCGG^CC
HgaI         2576  GCGTC                GACGC(5/10)
Hin1I        2575  GGCGTC               G[GA]^CG[CT]C
MseI         1645  TTAA                 T^TAA
NaeI         2108  GCCGGC               GCC^GGC
NdeI         1862  CATATG               CA^TATG
NgoMIV       2108  GCCGGC               G^CCGGC
NspI         2387  ACATGT               [GA]CATG^[CT]
NspV         1639  TTCGAA               TT^CGAA
PflFI         406  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     1363  AACGTT               AA^CGTT
RcaI         1394  TCATGA               T^CATGA
SacI         2333  GAGCTC               GAGCT^C
SapI         2224  GAAGAGC              GCTCTTC(1/4)
SbfI         2278  CCTGCAGG             CCTGCA^GG
ScaI         1273  AGTACT               AGT^ACT
SfuI         1639  TTCGAA               TT^CGAA
SmaI         1355  CCCGGG               CCC^GGG
SpeI          504  ACTAGT               A^CTAGT
Sse8387I     2278  CCTGCAGG             CCTGCA^GG
Tru9I        1645  TTAA                 T^TAA
Tth111I       406  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1355  CCCGGG               C^CCGGG
XmaI         1355  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII        137  TCCGGA               T^CCGGA
             2660  TCCGGA              
ApaI          696  GGGCCC               GGGCC^C
             2913  GGGCCC              
AvaI         1355  CCCGGG               C^[CT]CG[GA]G
             2113  CCCGAG              
BanI         1600  GGTGCC               G^G[CT][GA]CC
             2694  GGCACC              
BbvCI         735  CCTCAGC              CCTCAGC(-5/-2)
             1143  CCTCAGC             
BciVI         305  GTATCC               GTATCC(6/5)
             1176  GTATCC              
BfaI          505  CTAG                 C^TAG
             1468  CTAG                
BglI          631  GCCTTTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2229  GCCCAATGGGC         
BsaAI        1829  TACGTG               [CT]AC^GT[GA]
             2548  TACGTG              
BsaBI         274  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1407  GATGTCCATC          
BsaWI         137  TCCGGA               [AT]^CCGG[AT]
             2660  TCCGGA              
BseAI         137  TCCGGA               T^CCGGA
             2660  TCCGGA              
BsoBI        1355  CCCGGG               C^[CT]CG[GA]G
             2113  CCCGAG              
BspEI         137  TCCGGA               T^CCGGA
             2660  TCCGGA              
BspMI        2723  ACCTGC               ACCTGC(4/8)
             2281  GCAGGT              
BsrDI         711  CATTGC               GCAATG(2/0)
             1200  CATTGC              
BssHII       2013  GCGCGC               G^CGCGC
             2096  GCGCGC              
BstEII        492  GGTCACC              G^GT[ACGT]ACC
              873  GGTCACC             
BstPI         492  GGTCACC              G^GT[ACGT]ACC
              873  GGTCACC             
BstYI         353  GGATCT               [GA]^GATC[CT]
             1553  AGATCC              
DsaI          260  CCGTGG               C^C[GA][CT]GG
              683  CCGTGG              
EcoNI         315  CCTGGGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2724  CCTGCCTGAGG         
EcoO65I       492  GGTCACC              G^GT[ACGT]ACC
              873  GGTCACC             
HincII       1360  GTCAAC               GT[CT]^[GA]AC
             1918  GTTGAC              
HindII       1360  GTCAAC               GT[CT]^[GA]AC
             1918  GTTGAC              
MaeI          505  CTAG                 C^TAG
             1468  CTAG                
MamI          274  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1407  GATGTCCATC          
MflI          353  GGATCT               [GA]^GATC[CT]
             1553  AGATCC              
MroI          137  TCCGGA               T^CCGGA
             2660  TCCGGA              
XhoII         353  GGATCT               [GA]^GATC[CT]
             1553  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       BalI        BanII       BbsI        BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        
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