Restriction map for pF1KB7289
 (Circular) (Six-base) MAPSORT of: pF1KB7289.seq  Check: 1363  from: 1  to: 6077



pF1KB7289  6077 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:   3115    3115 
   Size:       6077
 
Acc65I G'GTAC_C

Cuts at:   3093    3093 
   Size:       6077
 
AclI AA'CG_TT

Cuts at:   1472    5917    1472 
   Size:       4445    1632
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    520     612     736    1162    2525    3902    4334    4687    5349 
   Size:         92     124     426    1363    1377     432     353     662

Cuts at:   5349     520 
   Size:       1248

  Fragments arranged by size: 

                1377    1363    1248     662     432     426     353     124
                  92
 
AfeI AGC'GCT

Cuts at:   1700    1700 
   Size:       6077
 
AflIII A'CryG_T

Cuts at:   2495    3429    5229    5510    2495 
   Size:        934    1800     281    3062

  Fragments arranged by size: 

                3062    1800     934     281
 
AleI CACnn'nnGTG

Cuts at:   1418    1735    1418 
   Size:        317    5760
 
AlwNI CAG_nnn'CTG

Cuts at:    591     830    1824    2386    2418    3382    4820     591 
   Size:        239     994     562      32     964    1438    1848

  Fragments arranged by size: 

                1848    1438     994     964     562     239      32
 
ApaI G_GGCC'C

Cuts at:    808    3025     808 
   Size:       2217    3860
 
ApaLI G'TGCA_C

Cuts at:   4915    4915 
   Size:       6077
 
ApoI r'AATT_y

Cuts at:    850    3081    5430    5686     850 
   Size:       2231    2349     256    1241

  Fragments arranged by size: 

                2349    2231    1241     256
 
AscI GG'CGCG_CC

Cuts at:   2204    2204 
   Size:       6077
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6077
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6077
 
AvaI C'yCGr_G

Cuts at:   1463    2221    3097    1463 
   Size:        758     876    4443

  Fragments arranged by size: 

                4443     876     758
 
BamHI G'GATC_C

Cuts at:   3102    3102 
   Size:       6077
 
BanI G'GyrC_C

Cuts at:   1708    2802    3093    3507    3755    3790    1708 
   Size:       1094     291     414     248      35    3995

  Fragments arranged by size: 

                3995    1094     414     291     248      35
 
BanII G_rGCy'C

Cuts at:    808    1187    2059    2198    2339    2445    2921    3025    3091 
   Size:        379     872     139     141     106     476     104      66

Cuts at:   3091    4121     808 
   Size:       1030    2764

  Fragments arranged by size: 

                2764    1030     872     476     379     141     139     106
                 104      66
 
BbeI G_GCGC'C

Cuts at:   3511    3759    3511 
   Size:        248    5829
 
BbsI GAAGACnn'nnnn_

Cuts at:    355     461     697    2264     355 
   Size:        106     236    1567    4168

  Fragments arranged by size: 

                4168    1567     236     106
 
BbvCI CC'TCA_GC

Cuts at:    844    1252     844 
   Size:        408    5669
 
BciVI GTATCCnnnnn_n'

Cuts at:    424    1295    3975    5031    6077     424 
   Size:        871    2680    1056    1046     424

  Fragments arranged by size: 

                2680    1056    1046     871     424
 
BclI T'GATC_A

Cuts at:   3597    3597 
   Size:       6077
 
BglI GCCn_nnn'nGGC

Cuts at:    745    2343     745 
   Size:       1598    4479
 
BglII A'GATC_T

Cuts at:   3592    5629    3592 
   Size:       2037    4040
 
BlpI GC'TnA_GC

Cuts at:   1268    1900    2536    3189    1268 
   Size:        632     636     653    4156

  Fragments arranged by size: 

                4156     653     636     632
 
Bme1580I G_kGCm'C

Cuts at:    808    2726    2805    3025    3702    3795    4919     808 
   Size:       1918      79     220     677      93    1124    1966

  Fragments arranged by size: 

                1966    1918    1124     677     220      93      79
 
BmrI ACTGGGnnnn_n'

Cuts at:    162    3289    3370    3705    5849     162 
   Size:       3127      81     335    2144     390

  Fragments arranged by size: 

                3127    2144     390     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1002    4444    1002 
   Size:       3442    2635
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    217     342     525    1035    1373    1978    2195    2716    2989 
   Size:        125     183     510     338     605     217     521     273

Cuts at:   2989     217 
   Size:       3305

  Fragments arranged by size: 

                3305     605     521     510     338     273     217     183
                 125
 
Bpu10I CC'TnA_GC

Cuts at:    844    1252    2108    2677     844 
   Size:        408     856     569    4244

  Fragments arranged by size: 

                4244     856     569     408
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3253    4132    4846    5144    3253 
   Size:        879     714     298    4186

  Fragments arranged by size: 

                4186     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2884    5781    2884 
   Size:       2897    3180
 
BsaAI yAC'GTr

Cuts at:   1939    2658    3430    1939 
   Size:        719     772    4586

  Fragments arranged by size: 

                4586     772     719
 
BsaBI GATnn'nnATC

Cuts at:      6     386    1519    3591    5628       6 
   Size:        380    1133    2072    2037     455

  Fragments arranged by size: 

                2072    2037    1133     455     380
 
BsaHI Gr'CG_yC

Cuts at:   2684    3508    3756    4458    5954    2684 
   Size:        824     248     702    1496    2807

  Fragments arranged by size: 

                2807    1496     824     702     248
 
BsaWI w'CCGG_w

Cuts at:    245    2768    3266    3787    4465    4876    5023    5297     245 
   Size:       2523     498     521     678     411     147     274    1025

  Fragments arranged by size: 

                2523    1025     678     521     498     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1711    1741    1711 
   Size:         30    6047
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    974    2713    2977    3013    3061     974 
   Size:       1739     264      36      48    3990

  Fragments arranged by size: 

                3990    1739     264      48      36
 
BseYI C'CCAG_C

Cuts at:    571    2052    2185    2433    3025    3502    4260    4925    5333 
   Size:       1481     133     248     592     477     758     665     408

Cuts at:   5333     571 
   Size:       1315

  Fragments arranged by size: 

                1481    1315     758     665     592     477     408     248
                 133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    180    2593    2761     180 
   Size:       2413     168    3496

  Fragments arranged by size: 

                3496    2413     168
 
BsiEI CG_ry'CG

Cuts at:    104    2071    3619    3665    4895     104 
   Size:       1967    1548      46    1230    1286

  Fragments arranged by size: 

                1967    1548    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   2445    3091    3869    4059    4919    2445 
   Size:        646     778     190     860    3603

  Fragments arranged by size: 

                3603     860     778     646     190
 
Bsp1286I G_dGCh'C

Cuts at:    808    1187    2059    2198    2339    2445    2726    2805    2921 
   Size:        379     872     139     141     106     281      79     116

Cuts at:   2921    3025    3091    3702    3795    3869    4059    4121    4919 
   Size:        104      66     611      93      74     190      62     798

Cuts at:   4919     808 
   Size:       1966

  Fragments arranged by size: 

                1966     872     798     611     379     281     190     141
                 139     116     106     104      93      79      74      66
                  62
 
BspEI T'CCGG_A

Cuts at:    245    2768     245 
   Size:       2523    3554
 
BspHI T'CATG_A

Cuts at:   1502    4509    1502 
   Size:       3007    3070
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2380    2840    3127    3341    3643    4024    2380 
   Size:        460     287     214     302     381    4433

  Fragments arranged by size: 

                4433     460     381     302     287     214
 
BsrBI CCG'CTC

Cuts at:     50    2067    4369    4423    5907    6072      50 
   Size:       2017    2302      54    1484     165      55

  Fragments arranged by size: 

                2302    2017    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    818    1307    3989     818 
   Size:        489    2682    2906

  Fragments arranged by size: 

                2906    2682     489
 
BsrFI r'CCGG_y

Cuts at:   2216    4075    4256    2216 
   Size:       1859     181    4037

  Fragments arranged by size: 

                4037    1859     181
 
BssHII G'CGCG_C

Cuts at:   2121    2204    2121 
   Size:         83    5994
 
BssSI C'ACGA_G

Cuts at:   4348    5056    4348 
   Size:        708    5369
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1278    3454    1278 
   Size:       2176    3901
 
BstBI TT'CG_AA

Cuts at:   1748    4438    1748 
   Size:       2690    3387
 
BstEII G'GTnAC_C

Cuts at:    600     981     600 
   Size:        381    5696
 
BstXI CCAn_nnnn'nTGG

Cuts at:    667    1788    1812     667 
   Size:       1121      24    4932

  Fragments arranged by size: 

                4932    1121      24
 
BstYI r'GATC_y

Cuts at:      1     461    1661    3102    3569    3592    3927    4173    4479 
   Size:        460    1200    1441     467      23     335     246     306

Cuts at:   4479    4491    4577    5629       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1441    1200    1052     467     460     449     335     306
                 246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2837    2837 
   Size:       6077
 
BtgI C'CryG_G

Cuts at:    107     368     791     107 
   Size:        261     423    5393

  Fragments arranged by size: 

                5393     423     261
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1198    4027    4208    4371    1198 
   Size:       2829     181     163    2904

  Fragments arranged by size: 

                2904    2829     181     163
 
BtsI GCAGTG_nn'

Cuts at:    192     786    1073    1094    2424    3320     192 
   Size:        594     287      21    1330     896    2949

  Fragments arranged by size: 

                2949    1330     896     594     287      21
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    133     168     978    1013     133 
   Size:         35     810      35    5197

  Fragments arranged by size: 

                5197     810      35      35
 
DraI TTT'AAA

Cuts at:   3076    3076 
   Size:       6077
 
DrdI GACnn_nn'nnGTC

Cuts at:   3783    5127    3783 
   Size:       1344    4733
 
EaeI y'GGCC_r

Cuts at:    294     746    1526    1613    1802    2355    3005    3662    3836 
   Size:        452     780      87     189     553     650     657     174

Cuts at:   3836    4227    4254    5599     294 
   Size:        391      27    1345     772

  Fragments arranged by size: 

                1345     780     772     657     650     553     452     391
                 189     174      87      27
 
EagI C'GGCC_G

Cuts at:   3662    3662 
   Size:       6077
 
EarI CTCTTCn'nnn_

Cuts at:    631     643    1498    2327    2501    2990    4100    4310     631 
   Size:         12     855     829     174     489    1110     210    2398

  Fragments arranged by size: 

                2398    1110     855     829     489     210     174      12
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2737    4285    5027    5173    5887    2737 
   Size:       1548     742     146     714    2927

  Fragments arranged by size: 

                2927    1548     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2443    3089    2443 
   Size:        646    5431
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    217     342     520     525     612     736    1035    1162    1373 
   Size:        125     178       5      87     124     299     127     211

Cuts at:   1373    1978    2195    2525    2716    2989    3902    4334    4687 
   Size:        605     217     330     191     273     913     432     353

Cuts at:   4687    5349     217 
   Size:        662     945

  Fragments arranged by size: 

                 945     913     662     605     432     353     330     299
                 273     217     211     191     178     127     125     124
                  87       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:    427    2836     427 
   Size:       2409    3668
 
EcoO109I rG'GnC_Cy

Cuts at:    228     259     804     805    1248    2082    3021    3022    3216 
   Size:         31     545       1     443     834     939       1     194

Cuts at:   3216     228 
   Size:       3089

  Fragments arranged by size: 

                3089     939     834     545     443     194      31       1
                   1
 
EcoRI G'AATT_C

Cuts at:   3081    3081 
   Size:       6077
 
EcoRV GAT'ATC

Cuts at:    903     903 
   Size:       6077
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4096    4096 
   Size:       6077
 
FseI GG_CCGG'CC

Cuts at:   2220    2220 
   Size:       6077
 
FspI TGC'GCA

Cuts at:   3858    3858 
   Size:       6077
 
HaeII r_GCGC'y

Cuts at:    635    1046    1702    2097    3511    3759    4989    5760     635 
   Size:        411     656     395    1414     248    1230     771     952

  Fragments arranged by size: 

                1414    1230     952     771     656     411     395     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2390    2422    2482    2514    2769    2801    3919    3949    3951 
   Size:         32      60      32     255      32    1118      30       2

Cuts at:   3951    3981    2390 
   Size:         30    4486

  Fragments arranged by size: 

                4486    1118     255      60      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1470    2028    3116    1470 
   Size:        558    1088    4431

  Fragments arranged by size: 

                4431    1088     558
 
KasI G'GCGC_C

Cuts at:   3507    3755    3507 
   Size:        248    5829
 
KpnI G_GTAC'C

Cuts at:   3097    3097 
   Size:       6077
 
MluI A'CGCG_T

Cuts at:   5510    5510 
   Size:       6077
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2051    2132    2939    4836    5020    2051 
   Size:         81     807    1897     184    3108

  Fragments arranged by size: 

                3108    1897     807     184      81
 
MscI TGG'CCA

Cuts at:    296     748    1528    1615    1804    2357    3838     296 
   Size:        452     780      87     189     553    1481    2535

  Fragments arranged by size: 

                2535    1481     780     553     452     189      87
 
MslI CAynn'nnrTG

Cuts at:    337     790     859    1418    1534    1564    1615    1735    1786 
   Size:        453      69     559     116      30      51     120      51

Cuts at:   1786    4066    4193     337 
   Size:       2280     127    2221

  Fragments arranged by size: 

                2280    2221     559     453     127     120     116      69
                  51      51      30
 
MspA1I CmG'CkG

Cuts at:    503    1447    1735    2535    2589    3188    3502    3862    4646 
   Size:        944     288     800      54     599     314     360     784

Cuts at:   4646    4891     503 
   Size:        245    1689

  Fragments arranged by size: 

                1689     944     800     784     599     360     314     288
                 245      54
 
NaeI GCC'GGC

Cuts at:   2218    4258    2218 
   Size:       2040    4037
 
NarI GG'CG_CC

Cuts at:   3508    3756    3508 
   Size:        248    5829
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       6077
 
NdeI CA'TA_TG

Cuts at:   1971    1971 
   Size:       6077
 
NgoMIV G'CCGG_C

Cuts at:   2216    4256    2216 
   Size:       2040    4037
 
NspI r_CATG'y

Cuts at:   2499    3130    3630    5233    2499 
   Size:        631     500    1603    3343

  Fragments arranged by size: 

                3343    1603     631     500
 
PciI A'CATG_T

Cuts at:   2495    5229    2495 
   Size:       2734    3343
 
PflMI CCAn_nnn'nTGG

Cuts at:    423    2136    2592     423 
   Size:       1713     456    3908

  Fragments arranged by size: 

                3908    1713     456
 
PfoI T'CCnGG_A

Cuts at:   1287    1287 
   Size:       6077
 
PmeI GTTT'AAAC

Cuts at:   3076    3076 
   Size:       6077
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4523    4555    4523 
   Size:         32    6045
 
PspOMI G'GGCC_C

Cuts at:    804    3021     804 
   Size:       2217    3860
 
PstI C_TGCA'G

Cuts at:    587    1829    2081    2093    2391    2820    3124    3809     587 
   Size:       1242     252      12     298     429     304     685    2855

  Fragments arranged by size: 

                2855    1242     685     429     304     298     252      12
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6077
 
PvuII CAG'CTG

Cuts at:    503    1447    1735    2535    2589    3502    3862     503 
   Size:        944     288     800      54     913     360    2718

  Fragments arranged by size: 

                2718     944     913     800     360     288      54
 
RsrII CG'GwC_CG

Cuts at:   4272    4272 
   Size:       6077
 
SacI G_AGCT'C

Cuts at:   2445    3091    2445 
   Size:        646    5431
 
SalI G'TCGA_C

Cuts at:   3114    3114 
   Size:       6077
 
SapI GCTCTTCn'nnn_

Cuts at:   2327    4100    4310    2327 
   Size:       1773     210    4094

  Fragments arranged by size: 

                4094    1773     210
 
SbfI CC_TGCA'GG

Cuts at:   2391    3124    2391 
   Size:        733    5344
 
ScaI AGT'ACT

Cuts at:   1383    5594    1383 
   Size:       4211    1866
 
SfcI C'TryA_G

Cuts at:     33     583    1825    2077    2089    2387    2704    2816    3120 
   Size:        550    1242     252      12     298     317     112     304

Cuts at:   3120    3805    4773    4964      33 
   Size:        685     968     191    1146

  Fragments arranged by size: 

                1242    1146     968     685     550     317     304     298
                 252     191     112      12
 
SfoI GGC'GCC

Cuts at:   3509    3757    3509 
   Size:        248    5829
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6077
 
SmaI CCC'GGG

Cuts at:   1465    3099    1465 
   Size:       1634    4443
 
SmlI C'TyrA_G

Cuts at:   3232    4147    4861    5123    3232 
   Size:        915     714     262    4186

  Fragments arranged by size: 

                4186     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67     612      67 
   Size:        545    5532
 
SphI G_CATG'C

Cuts at:   3130    3630    3130 
   Size:        500    5577
 
StuI AGG'CCT

Cuts at:    541    2518    2847     541 
   Size:       1977     329    3771

  Fragments arranged by size: 

                3771    1977     329
 
StyI C'CwwG_G

Cuts at:    107     749    1884    2637    3211    3565     107 
   Size:        642    1135     753     574     354    2619

  Fragments arranged by size: 

                2619    1135     753     642     574     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1395    3660    3793    4252    4461    1395 
   Size:       2265     133     459     209    3011

  Fragments arranged by size: 

                3011    2265     459     209     133
 
TatI w'GTAC_w

Cuts at:   1381    5592    1381 
   Size:       4211    1866
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    419     451    1164    1196    1711    1743    5594    5626     419 
   Size:         32     713      32     515      32    3851      32     870

  Fragments arranged by size: 

                3851     870     713     515      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:    517    3874     517 
   Size:       3357    2720
 
XbaI T'CTAG_A

Cuts at:   3108    3108 
   Size:       6077
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    366    1119    2484    3572     366 
   Size:        753    1365    1088    2871

  Fragments arranged by size: 

                2871    1365    1088     753
 
XmaI C'CCGG_G

Cuts at:   1463    3097    1463 
   Size:       1634    4443
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AleI    AlwNI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AhdI     AlfI     AloI    AvrII
     BaeI     BcgI    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsmBI
    BsrGI  BstZ17I     ClaI   DraIII    FspAI  HindIII     HpaI     MfeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI    PpuMI
    PshAI     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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