Restriction map for pF1KB7287
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        Asp718I     
AspEI       AvrII       BbeI        BbrPI       BciVI       
BfrI        BlnI        BlpI        Bpu1102I    BsiWI       
BsmBI       Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
ClaI        CpoI        DraII       DraIII      Eam1105I    
EcoO109I    EcoT22I     HpaI        KasI        KpnI        
MfeI        MluI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RsrII       
SalI        SbfI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI         2626  GTCTAC               GT^[AC][GT]AC
AcyI           60  GACGCC               G[GA]^CG[CT]C
AflIII        716  ACACGT               A^C[GA][CT]GT
Asp700I      1719  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1284  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         470  TGCGCA               TGC^GCA
BcgI         1320  CGAGCTCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        3455  AGATCT               A^GATCT
BsaHI          60  GACGCC               G[GA]^CG[CT]C
BspHI        2591  TCATGA               T^CATGA
BssSI        1117  CTCGTG               CACGAG(-5/-1)
BstAPI         99  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2867  CCAGCCATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         2083  TTTAAA               TTT^AAA
DrdI         3684  GACTTTGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2889  CCTAGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1723  GAATTC               G^AATTC
FseI          882  GGCCGGCC             GGCCGG^CC
FspI          470  TGCGCA               TGC^GCA
HgaI           60  GACGC                GACGC(5/10)
Hin1I          60  GACGCC               G[GA]^CG[CT]C
HincII       1510  GTTGAC               GT[CT]^[GA]AC
HindII       1510  GTTGAC               GT[CT]^[GA]AC
KspI         3578  CCGCGG               CCGC^GG
PacI         1756  TTAATTAA             TTAAT^TAA
PflFI        1284  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2591  TCATGA               T^CATGA
SacII        3578  CCGCGG               CCGC^GG
SexAI         956  ACCAGGT              A^CC[AT]GGT
SfiI          873  GGCCATCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          285  CCCGGG               CCC^GGG
Tth111I      1284  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         285  CCCGGG               C^CCGGG
XmaI          285  CCCGGG               C^CCGGG
XmnI         1719  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccII         237  CGCG                 CG^CG
             3579  CGCG                
AcsI         1723  GAATTC               [GA]^AATT[CT]
             1932  AAATTC              
Aor51HI       143  AGCGCT               AGC^GCT
              512  AGCGCT              
ApaI          282  GGGCCC               GGGCC^C
             1031  GGGCCC              
ApoI         1723  GAATTC               [GA]^AATT[CT]
             1932  AAATTC              
AseI         2332  ATTAAT               AT^TAAT
             2383  ATTAAT              
AsnI         2332  ATTAAT               AT^TAAT
             2383  ATTAAT              
BamHI        1861  GGATCC               G^GATCC
             3386  GGATCC              
BbvCI        2465  CCTCAGC              CCTCAGC(-5/-2)
             2826  GCTGAGG             
BfaI          407  CTAG                 C^TAG
             2890  CTAG                
BsaAI         717  CACGTA               [CT]AC^GT[GA]
              817  TACGTG              
BsiEI          68  CGGCCG               CG[GA][CT]^CG
               94  CGGCCG              
BsmI         3016  GAATGC               GAATGC(1/-1)
             3237  GCATTC              
BsrDI        3042  CATTGC               GCAATG(2/0)
             3153  GCAATG              
BstEII        741  GGTCACC              G^GT[ACGT]ACC
             2532  GGTGACC             
BstPI         741  GGTCACC              G^GT[ACGT]ACC
             2532  GGTGACC             
BstUI         237  CGCG                 CG^CG
             3579  CGCG                
EagI           68  CGGCCG               C^GGCCG
               94  CGGCCG              
EclXI          68  CGGCCG               C^GGCCG
               94  CGGCCG              
Eco47III      143  AGCGCT               AGC^GCT
              512  AGCGCT              
Eco52I         68  CGGCCG               C^GGCCG
               94  CGGCCG              
EcoO65I       741  GGTCACC              G^GT[ACGT]ACC
             2532  GGTGACC             
EcoRV        1798  GATATC               GAT^ATC
             2482  GATATC              
HindIII      1043  AAGCTT               A^AGCTT
             1967  AAGCTT              
MaeI          407  CTAG                 C^TAG
             2890  CTAG                
MvnI          237  CGCG                 CG^CG
             3579  CGCG                
NaeI           70  GCCGGC               GCC^GGC
              883  GCCGGC              
NgoMIV         70  GCCGGC               G^CCGGC
              883  GCCGGC              
NspI         1974  GCATGT               [GA]CATG^[CT]
             2846  ACATGC              
PshBI        2332  ATTAAT               AT^TAAT
             2383  ATTAAT              
SapI          822  GCTCTTC              GCTCTTC(1/4)
              831  GCTCTTC             
ScaI         2501  AGTACT               AGT^ACT
             3566  AGTACT              
SmlI          600  CTTGAG               C^T[CT][GA]AG
              631  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccIII      AciI        AfaI        AluI        AlwI        
AlwNI       AocI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BclI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaBI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspEI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EarI        Eco81I      
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MroI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       
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