Restriction map for pF1KB7287
 (Linear) (Six-base) MAPSORT of: KIBB7287.seq  Check: 4124  from: 1  to: 3753



KIBB7287.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     316    1199    3753 
   Size:        316     883    2554

  Fragments arranged by size: 

                2554     883     316
 
AccI GT'mk_AC

Cuts at:      0    2627    3753 
   Size:       2627    1126
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1814    1877    2590    3439    3746    3753 
   Size:       1814      63     713     849     307       7

  Fragments arranged by size: 

                1814     849     713     307      63       7
 
AfeI AGC'GCT

Cuts at:      0     145     514    3753 
   Size:        145     369    3239

  Fragments arranged by size: 

                3239     369     145
 
AflIII A'CryG_T

Cuts at:      0     716    3753 
   Size:        716    3037
 
AleI CACnn'nnGTG

Cuts at:      0     748    1442    2518    3753 
   Size:        748     694    1076    1235

  Fragments arranged by size: 

                1235    1076     748     694
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     916    3753 
   Size:        916    2837
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2116    2148    3753 
   Size:       2116      32    1605

  Fragments arranged by size: 

                2116    1605      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105     308     771    1302    2754    3712    3753 
   Size:        105     203     463     531    1452     958      41

  Fragments arranged by size: 

                1452     958     531     463     203     105      41
 
ApaI G_GGCC'C

Cuts at:      0     286    1035    3753 
   Size:        286     749    2718

  Fragments arranged by size: 

                2718     749     286
 
ApoI r'AATT_y

Cuts at:      0    1723    1932    3753 
   Size:       1723     209    1821

  Fragments arranged by size: 

                1821    1723     209
 
AseI AT'TA_AT

Cuts at:      0    2333    2384    3753 
   Size:       2333      51    1369

  Fragments arranged by size: 

                2333    1369      51
 
AvaI C'yCGr_G

Cuts at:      0     192     285     488    3753 
   Size:        192      93     203    3265

  Fragments arranged by size: 

                3265     203     192      93
 
BamHI G'GATC_C

Cuts at:      0    1861    3386    3753 
   Size:       1861    1525     367

  Fragments arranged by size: 

                1861    1525     367
 
BanI G'GyrC_C

Cuts at:      0     158     313     892    2395    3730    3753 
   Size:        158     155     579    1503    1335      23

  Fragments arranged by size: 

                1503    1335     579     158     155      23
 
BanII G_rGCy'C

Cuts at:      0       8     286     797    1035    1325    1421    1822    1873 
   Size:          8     278     511     238     290      96     401      51

Cuts at:   1873    3720    3753 
   Size:       1847      33

  Fragments arranged by size: 

                1847     511     401     290     278     238      96      51
                  33       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     315    2502    3077    3167    3558    3753 
   Size:        315    2187     575      90     391     195

  Fragments arranged by size: 

                2187     575     391     315     195      90
 
BbvCI CC'TCA_GC

Cuts at:      0    2466    2827    3753 
   Size:       2466     361     926

  Fragments arranged by size: 

                2466     926     361
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1309    1343    3753 
   Size:       1309      34    2410

  Fragments arranged by size: 

                2410    1309      34
 
BclI T'GATC_A

Cuts at:      0    1157    1466    2588    3753 
   Size:       1157     309    1122    1165

  Fragments arranged by size: 

                1165    1157    1122     309
 
BglI GCCn_nnn'nGGC

Cuts at:      0      29     102     880    3753 
   Size:         29      73     778    2873

  Fragments arranged by size: 

                2873     778      73      29
 
BglII A'GATC_T

Cuts at:      0    3455    3753 
   Size:       3455     298
 
Bme1580I G_kGCm'C

Cuts at:      0     161     286     405     895    1035    3753 
   Size:        161     125     119     490     140    2718

  Fragments arranged by size: 

                2718     490     161     140     125     119
 
BmgBI CAC'GTC

Cuts at:      0     567    3753 
   Size:        567    3186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1072    1108    1646    1887    2076    2119    2776    3734 
   Size:       1072      36     538     241     189      43     657     958

Cuts at:   3734    3753 
   Size:         19

  Fragments arranged by size: 

                1072     958     657     538     241     189      43      36
                  19
 
Bpu10I CC'TnA_GC

Cuts at:      0    1295    1415    2466    2827    3753 
   Size:       1295     120    1051     361     926

  Fragments arranged by size: 

                1295    1051     926     361     120
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     621    3753 
   Size:        616       5    3132

  Fragments arranged by size: 

                3132     616       5
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1462    2332    2614    3397    3753 
   Size:       1462     870     282     783     356

  Fragments arranged by size: 

                1462     870     783     356     282
 
BsaAI yAC'GTr

Cuts at:      0     719     819    3753 
   Size:        719     100    2934

  Fragments arranged by size: 

                2934     719     100
 
BsaBI GATnn'nnATC

Cuts at:      0    1216    1525    2599    3268    3753 
   Size:       1216     309    1074     669     485

  Fragments arranged by size: 

                1216    1074     669     485     309
 
BsaHI Gr'CG_yC

Cuts at:      0      61    3753 
   Size:         61    3692
 
BsaWI w'CCGG_w

Cuts at:      0    1829    2312    2852    3260    3753 
   Size:       1829     483     540     408     493

  Fragments arranged by size: 

                1829     540     493     483     408
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     333     363     694     724    1824    1854    2092    2122 
   Size:        333      30     331      30    1100      30     238      30

Cuts at:   2122    3753 
   Size:       1631

  Fragments arranged by size: 

                1631    1100     333     331     238      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1812    2818    2926    3415    3753 
   Size:       1812    1006     108     489     338

  Fragments arranged by size: 

                1812    1006     489     338     108
 
BseYI C'CCAG_C

Cuts at:      0     279     645    1034    2866    3697    3753 
   Size:        279     366     389    1832     831      56

  Fragments arranged by size: 

                1832     831     389     366     279      56
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    2236    2561    3753 
   Size:        373    1863     325    1192

  Fragments arranged by size: 

                1863    1192     373     325
 
BsiEI CG_ry'CG

Cuts at:      0      71      97    3753 
   Size:         71      26    3656

  Fragments arranged by size: 

                3656      71      26
 
BsiHKAI G_wGCw'C

Cuts at:      0       8     824    1133    1190    1325    1822    2105    3186 
   Size:          8     816     309      57     135     497     283    1081

Cuts at:   3186    3753 
   Size:        567

  Fragments arranged by size: 

                1081     816     567     497     309     283     135      57
                   8
 
BsmI GAATG_Cn'

Cuts at:      0    3022    3237    3753 
   Size:       3022     215     516

  Fragments arranged by size: 

                3022     516     215
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     161     286     405     797     824     895    1035 
   Size:          8     153     125     119     392      27      71     140

Cuts at:   1035    1133    1190    1325    1421    1822    1873    2105    3186 
   Size:         98      57     135      96     401      51     232    1081

Cuts at:   3186    3720    3753 
   Size:        534      33

  Fragments arranged by size: 

                1081     534     401     392     232     153     140     135
                 125     119      98      96      71      57      51      33
                  27       8
 
BspEI T'CCGG_A

Cuts at:      0    2312    2852    3260    3753 
   Size:       2312     540     408     493

  Fragments arranged by size: 

                2312     540     493     408
 
BspHI T'CATG_A

Cuts at:      0    2591    3753 
   Size:       2591    1162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     316     731     897    1199    3753 
   Size:        316     415     166     302    2554

  Fragments arranged by size: 

                2554     415     316     302     166
 
BsrBI CCG'CTC

Cuts at:      0     344     630     864    1113    3753 
   Size:        344     286     234     249    2640

  Fragments arranged by size: 

                2640     344     286     249     234
 
BsrDI GCAATG_nn'

Cuts at:      0    3041    3160    3753 
   Size:       3041     119     593

  Fragments arranged by size: 

                3041     593     119
 
BsrFI r'CCGG_y

Cuts at:      0      70     658     883    3677    3753 
   Size:         70     588     225    2794      76

  Fragments arranged by size: 

                2794     588     225      76      70
 
BssSI C'ACGA_G

Cuts at:      0    1117    3753 
   Size:       1117    2636
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     105    3753 
   Size:        105    3648
 
BstEII G'GTnAC_C

Cuts at:      0     741    2532    3753 
   Size:        741    1791    1221

  Fragments arranged by size: 

                1791    1221     741
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2874    3753 
   Size:       2874     879
 
BstYI r'GATC_y

Cuts at:      0     767    1211    1861    3386    3455    3753 
   Size:        767     444     650    1525      69     298

  Fragments arranged by size: 

                1525     767     650     444     298      69
 
Bsu36I CC'TnA_GG

Cuts at:      0    1729    1769    1991    3753 
   Size:       1729      40     222    1762

  Fragments arranged by size: 

                1762    1729     222      40
 
BtgI C'CryG_G

Cuts at:      0     620    1704    2528    2742    3276    3578    3753 
   Size:        620    1084     824     214     534     302     175

  Fragments arranged by size: 

                1084     824     620     534     302     214     175
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1207    3753 
   Size:       1207    2546
 
BtsI GCAGTG_nn'

Cuts at:      0     354     980    1359    1437    2585    2701    3753 
   Size:        354     626     379      78    1148     116    1052

  Fragments arranged by size: 

                1148    1052     626     379     354     116      78
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2649    2684    3753 
   Size:       2649      35    1069

  Fragments arranged by size: 

                2649    1069      35
 
DraI TTT'AAA

Cuts at:      0    2085    3753 
   Size:       2085    1668
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3690    3753 
   Size:       3690      63
 
EaeI y'GGCC_r

Cuts at:      0      68      94     752     881    2525    2714    3122    3381 
   Size:         68      26     658     129    1644     189     408     259

Cuts at:   3381    3575    3753 
   Size:        194     178

  Fragments arranged by size: 

                1644     658     408     259     194     189     178     129
                  68      26
 
EagI C'GGCC_G

Cuts at:      0      68      94    3753 
   Size:         68      26    3659

  Fragments arranged by size: 

                3659      68      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     829     838    1691    2046    3510    3753 
   Size:        829       9     853     355    1464     243

  Fragments arranged by size: 

                1464     853     829     355     243       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3696    3753 
   Size:       3696      57
 
EcoICRI GAG'CTC

Cuts at:      0       6    1323    1820    3753 
   Size:          6    1317     497    1933

  Fragments arranged by size: 

                1933    1317     497       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1072    1108    1646    1814    1877    1887    2076    2119 
   Size:       1072      36     538     168      63      10     189      43

Cuts at:   2119    2590    2776    3439    3734    3746    3753 
   Size:        471     186     663     295      12       7

  Fragments arranged by size: 

                1072     663     538     471     295     189     186     168
                  63      43      36      12      10       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2893    3753 
   Size:       2893     860
 
EcoRI G'AATT_C

Cuts at:      0    1723    3753 
   Size:       1723    2030
 
EcoRV GAT'ATC

Cuts at:      0    1800    2484    3753 
   Size:       1800     684    1269

  Fragments arranged by size: 

                1800    1269     684
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2160    3753 
   Size:       2160    1593
 
FseI GG_CCGG'CC

Cuts at:      0     887    3753 
   Size:        887    2866
 
FspI TGC'GCA

Cuts at:      0     472    3753 
   Size:        472    3281
 
HaeII r_GCGC'y

Cuts at:      0      89     147     265     516    1041    3308    3753 
   Size:         89      58     118     251     525    2267     445

  Fragments arranged by size: 

                2267     525     445     251     118      89      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     694     726    1573    1605    2045    2077    2473    2505 
   Size:        694      32     847      32     440      32     396      32

Cuts at:   2505    2968    3000    3440    3472    3753 
   Size:        463      32     440      32     281

  Fragments arranged by size: 

                 847     694     463     440     440     396     281      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1512    3753 
   Size:       1512    2241
 
HindIII A'AGCT_T

Cuts at:      0    1043    1967    3753 
   Size:       1043     924    1786

  Fragments arranged by size: 

                1786    1043     924
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2308    3546    3753 
   Size:       2308    1238     207

  Fragments arranged by size: 

                2308    1238     207
 
MscI TGG'CCA

Cuts at:      0     754    2527    2716    3753 
   Size:        754    1773     189    1037

  Fragments arranged by size: 

                1773    1037     754     189
 
MslI CAynn'nnrTG

Cuts at:      0     748     988    1226    1442    2518    3118    3124    3179 
   Size:        748     240     238     216    1076     600       6      55

Cuts at:   3179    3753 
   Size:        574

  Fragments arranged by size: 

                1076     748     600     574     240     238     216      55
                   6
 
MspA1I CmG'CkG

Cuts at:      0     102     173     179     246     338     356     362     734 
   Size:        102      71       6      67      92      18       6     372

Cuts at:    734     858    1000    2470    2754    3580    3701    3753 
   Size:        124     142    1470     284     826     121      52

  Fragments arranged by size: 

                1470     826     372     284     142     124     121     102
                  92      71      67      52      18       6       6
 
NaeI GCC'GGC

Cuts at:      0      72     885    3753 
   Size:         72     813    2868

  Fragments arranged by size: 

                2868     813      72
 
NcoI C'CATG_G

Cuts at:      0     620    1704    2528    2742    3753 
   Size:        620    1084     824     214    1011

  Fragments arranged by size: 

                1084    1011     824     620     214
 
NgoMIV G'CCGG_C

Cuts at:      0      70     883    3753 
   Size:         70     813    2870

  Fragments arranged by size: 

                2870     813      70
 
NspI r_CATG'y

Cuts at:      0    1978    2850    3753 
   Size:       1978     872     903

  Fragments arranged by size: 

                1978     903     872
 
PacI TTA_AT'TAA

Cuts at:      0    1760    3753 
   Size:       1760    1993
 
PasI CC'CwG_GG

Cuts at:      0    1636    3753 
   Size:       1636    2117
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     771     963    3012    3753 
   Size:        771     192    2049     741

  Fragments arranged by size: 

                2049     771     741     192
 
PfoI T'CCnGG_A

Cuts at:      0     326    1049    1148    1864    3753 
   Size:        326     723      99     716    1889

  Fragments arranged by size: 

                1889     723     716     326      99
 
PsiI TTA'TAA

Cuts at:      0    2291    3753 
   Size:       2291    1462
 
PspOMI G'GGCC_C

Cuts at:      0     282    1031    3753 
   Size:        282     749    2722

  Fragments arranged by size: 

                2722     749     282
 
PstI C_TGCA'G

Cuts at:      0     728     908    1196    3753 
   Size:        728     180     288    2557

  Fragments arranged by size: 

                2557     728     288     180
 
PvuII CAG'CTG

Cuts at:      0     246    1000    2470    2754    3753 
   Size:        246     754    1470     284     999

  Fragments arranged by size: 

                1470     999     754     284     246
 
SacI G_AGCT'C

Cuts at:      0       8    1325    1822    3753 
   Size:          8    1317     497    1931

  Fragments arranged by size: 

                1931    1317     497       8
 
SacII CC_GC'GG

Cuts at:      0    3581    3753 
   Size:       3581     172
 
SapI GCTCTTCn'nnn_

Cuts at:      0     829     838    3753 
   Size:        829       9    2915

  Fragments arranged by size: 

                2915     829       9
 
ScaI AGT'ACT

Cuts at:      0    2503    3568    3753 
   Size:       2503    1065     185

  Fragments arranged by size: 

                2503    1065     185
 
SexAI A'CCwGG_T

Cuts at:      0     956    3753 
   Size:        956    2797
 
SfcI C'TryA_G

Cuts at:      0     438     724     843     904    1192    3753 
   Size:        438     286     119      61     288    2561

  Fragments arranged by size: 

                2561     438     288     286     119      61
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     880    3753 
   Size:        880    2873
 
SmaI CCC'GGG

Cuts at:      0     287    3753 
   Size:        287    3466
 
SmlI C'TyrA_G

Cuts at:      0     600     631    3753 
   Size:        600      31    3122

  Fragments arranged by size: 

                3122     600      31
 
StuI AGG'CCT

Cuts at:      0     782    2636    3134    3753 
   Size:        782    1854     498     619

  Fragments arranged by size: 

                1854     782     619     498
 
StyI C'CwwG_G

Cuts at:      0     620    1704    2528    2690    2742    3753 
   Size:        620    1084     824     162      52    1011

  Fragments arranged by size: 

                1084    1011     824     620     162      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     738     778    2383    2915    3089    3711    3753 
   Size:        738      40    1605     532     174     622      42

  Fragments arranged by size: 

                1605     738     622     532     174      42      40
 
TatI w'GTAC_w

Cuts at:      0     728    2501    2759    2790    3566    3753 
   Size:        728    1773     258      31     776     187

  Fragments arranged by size: 

                1773     776     728     258     187      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     333     365    1824    1856    3753 
   Size:        333      32    1459      32    1897

  Fragments arranged by size: 

                1897    1459     333      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1287    3753 
   Size:       1287    2466
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     768    1083    1662    2724    3753 
   Size:        768     315     579    1062    1029

  Fragments arranged by size: 

                1062    1029     768     579     315
 
XmaI C'CCGG_G

Cuts at:      0     285    3753 
   Size:        285    3468
 
XmnI GAAnn'nnTTC

Cuts at:      0    1723    3753 
   Size:       1723    2030
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI     BclI     BglI    BglII Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    HaeII    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     PacI     PasI    PflMI     PfoI     PsiI   PspOMI
     PstI    PvuII     SacI    SacII     SapI     ScaI    SexAI     SfcI
     SfiI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI    ApaLI     AscI
    AsiSI    AvrII     BaeI     BbeI    BciVI    BfrBI     BlpI     BmrI
     BmtI     BplI    BsiWI    BsmBI    BsrGI   BssHII    BstBI  BstZ17I
     ClaI   DraIII EcoO109I    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SbfI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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