Restriction map for pF1KB7273
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AccIII      AclI        AgeI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AspI        AvaI        
AviII       BamHI       BbrPI       BbvCI       BcgI        
BcnI        BglII       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       BspEI       
BsrBI       BsrFI       BssHII      BssSI       BstBI       
Cfr10I      CpoI        DraII       DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoO109I    FseI        
FspI        KspI        MluI        MroI        NaeI        
NciI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AatII        1184  GACGTC               GACGT^C
Acc65I       3807  GGTACC               G^GTACC
AccII        3812  CGCG                 CG^CG
AflII        2749  CTTAAG               C^TTAAG
AhdI           62  GACTGTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3143  CCTCAGG              CC^T[ACGT]AGG
AseI         1818  ATTAAT               AT^TAAT
AsnI         1818  ATTAAT               AT^TAAT
Asp718I      3807  GGTACC               G^GTACC
AspEI          62  GACTGTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        3076  CCTAGG               C^CTAGG
BanII        3424  GAGCCC               G[GA]GC[CT]^C
BbeI            9  GGCGCC               GGCGC^C
BfrI         2749  CTTAAG               C^TTAAG
BglI         3128  GCCGACATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3076  CCTAGG               C^CTAGG
BlpI         1914  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1914  GCTGAGC              GC^T[ACGT]AGC
BsaAI        3759  TACGTG               [CT]AC^GT[GA]
BsaI           69  GGTCTC               GGTCTC(1/5)
Bsp1407I     3956  TGTACA               T^GTACA
BspDI        1279  ATCGAT               AT^CGAT
BspLU11I      521  ACATGT               A^CATGT
BspMI         945  ACCTGC               ACCTGC(4/8)
BsrGI        3956  TGTACA               T^GTACA
Bst1107I     2641  GTATAC               GTA^TAC
BstAPI        268  GCACAATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3160  GGTCACC              G^GT[ACGT]ACC
BstPI        3160  GGTCACC              G^GT[ACGT]ACC
BstUI        3812  CGCG                 CG^CG
BstXI        2272  CCAATTGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2641  GTATAC               GTA^TAC
Bsu36I       3143  CCTCAGG              CC^T[ACGT]AGG
CelII        1914  GCTGAGC              GC^T[ACGT]AGC
CfoI           10  GCGC                 GCG^C
ClaI         1279  ATCGAT               AT^CGAT
DraIII       2765  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2981  CCATGG               C^C[GA][CT]GG
Eam1105I       62  GACTGTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3143  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      3160  GGTCACC              G^GT[ACGT]ACC
EcoRV         715  GATATC               GAT^ATC
FauI           57  GCGGG                CCCGC(4/6)
HaeII           9  GGCGCC               [GA]GCGC^[CT]
HapII         456  CCGG                 C^CGG
HhaI           10  GCGC                 GCG^C
HinP1I         10  GCGC                 G^CGC
HindIII      3198  AAGCTT               A^AGCTT
HpaI         1830  GTTAAC               GTT^AAC
HpaII         456  CCGG                 C^CGG
KasI            9  GGCGCC               G^GCGCC
KpnI         3807  GGTACC               GGTAC^C
MspI          456  CCGG                 C^CGG
MvnI         3812  CGCG                 CG^CG
NarI            9  GGCGCC               GG^CGCC
NcoI         2981  CCATGG               C^CATGG
NdeI         1840  CATATG               CA^TATG
PshBI        1818  ATTAAT               AT^TAAT
SalI         1187  GTCGAC               G^TCGAC
SfoI            9  GGCGCC               GGC^GCC
SmlI         2749  CTTAAG               C^T[CT][GA]AG
SphI         1527  GCATGC               GCATG^C
SspBI        3956  TGTACA               T^GTACA
StuI         3115  AGGCCT               AGG^CCT
XbaI         1641  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         1187  GTCGAC               GT^[AC][GT]AC
             2641  GTATAC              
AcyI            9  GGCGCC               G[GA]^CG[CT]C
             1184  GACGTC              
AflIII        521  ACATGT               A^C[GA][CT]GT
             3760  ACGTGT              
AlwNI        3278  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3327  CAGCTCCTG           
Asp700I       676  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2589  GAATAATTTC          
AspHI        1519  GAGCAC               G[AT]GC[AT]^C
             3826  GTGCTC              
BalI          938  TGGCCA               TGG^CCA
             1419  TGGCCA              
BciVI         376  GTATCC               GTATCC(6/5)
             1543  GTATCC              
BsaBI        3313  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3505  GATGATGATC          
BsaHI           9  GGCGCC               G[GA]^CG[CT]C
             1184  GACGTC              
BsiHKAI      1519  GAGCAC               G[AT]GC[AT]^C
             3826  GTGCTC              
BsmFI          60  GGGAC                GGGAC(10/14)
             1335  GGGAC               
BsrDI         258  CATTGC               GCAATG(2/0)
              972  CATTGC              
EaeI          938  TGGCCA               [CT]^GGCC[GA]
             1419  TGGCCA              
EcoRI        1321  GAATTC               G^AATTC
             3888  GAATTC              
HgaI         1963  GACGC                GACGC(5/10)
             3813  GCGTC               
Hin1I           9  GGCGCC               G[GA]^CG[CT]C
             1184  GACGTC              
HincII       1187  GTCGAC               GT[CT]^[GA]AC
             1830  GTTAAC              
HindII       1187  GTCGAC               GT[CT]^[GA]AC
             1830  GTTAAC              
MamI         3313  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3505  GATGATGATC          
MluNI         938  TGGCCA               TGG^CCA
             1419  TGGCCA              
MscI          938  TGGCCA               TGG^CCA
             1419  TGGCCA              
PstI          947  CTGCAG               CTGCA^G
             2671  CTGCAG              
ScaI         1636  AGTACT               AGT^ACT
             2544  AGTACT              
XcmI          241  CCAATTGATAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2269  CCACCAATTGAATGG     
XmnI          676  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2589  GAATAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanI        BbsI        BbvI        
BclI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       Bsp1286I    BspHI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        EarI        EcoNI       
EcoRII      EcoT14I     EcoT22I     FbaI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MslI        MspA1I      MunI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PvuII       RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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