Restriction map for pF1KB7273
 (Linear) (Six-base) MAPSORT of: KIBB7273.seq  Check: 4168  from: 1  to: 3996



KIBB7273.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AatII G_ACGT'C

Cuts at:      0    1188    3996 
   Size:       1188    2808
 
AccI GT'mk_AC

Cuts at:      0    1188    2642    3996 
   Size:       1188    1454    1354

  Fragments arranged by size: 

                1454    1354    1188
 
Acc65I G'GTAC_C

Cuts at:      0    3807    3996 
   Size:       3807     189
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15    1607    1730    2827    3275    3685    3996 
   Size:         15    1592     123    1097     448     410     311

  Fragments arranged by size: 

                1592    1097     448     410     311     123      15
 
AflII C'TTAA_G

Cuts at:      0    2749    3996 
   Size:       2749    1247
 
AflIII A'CryG_T

Cuts at:      0     521    3760    3996 
   Size:        521    3239     236

  Fragments arranged by size: 

                3239     521     236
 
AhdI GACnn_n'nnGTC

Cuts at:      0      67    3996 
   Size:         67    3929
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1544    3996 
   Size:       1544    2452
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3283    3332    3996 
   Size:       3283      49     664

  Fragments arranged by size: 

                3283     664      49
 
ApoI r'AATT_y

Cuts at:      0    1160    1265    1321    1471    1493    1940    2442    2451 
   Size:       1160     105      56     150      22     447     502       9

Cuts at:   2451    2523    2564    2628    2690    2903    3480    3768    3888 
   Size:         72      41      64      62     213     577     288     120

Cuts at:   3888    3996 
   Size:        108

  Fragments arranged by size: 

                1160     577     502     447     288     213     150     120
                 108     105      72      64      62      56      41      22
                   9
 
AseI AT'TA_AT

Cuts at:      0    1819    3996 
   Size:       1819    2177
 
AvrII C'CTAG_G

Cuts at:      0    3076    3996 
   Size:       3076     920
 
BanI G'GyrC_C

Cuts at:      0       9    1026    1354    3807    3996 
   Size:          9    1017     328    2453     189

  Fragments arranged by size: 

                2453    1017     328     189       9
 
BanII G_rGCy'C

Cuts at:      0    3428    3996 
   Size:       3428     568
 
BbeI G_GCGC'C

Cuts at:      0      13    3996 
   Size:         13    3983
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     232     791    1103    1766    2815    3996 
   Size:        232     559     312     663    1049    1181

  Fragments arranged by size: 

                1181    1049     663     559     312     232
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     387    1554    3996 
   Size:        387    1167    2442

  Fragments arranged by size: 

                2442    1167     387
 
BclI T'GATC_A

Cuts at:      0     528     656    1397    3465    3996 
   Size:        528     128     741    2068     531

  Fragments arranged by size: 

                2068     741     531     528     128
 
BfrBI ATG'CAT

Cuts at:      0     179    1531    2507    3996 
   Size:        179    1352     976    1489

  Fragments arranged by size: 

                1489    1352     976     179
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3134    3996 
   Size:       3134     862
 
BlpI GC'TnA_GC

Cuts at:      0    1915    3996 
   Size:       1915    2081
 
BmgBI CAC'GTC

Cuts at:      0    1015    2350    3996 
   Size:       1015    1335    1646

  Fragments arranged by size: 

                1646    1335    1015
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3724    3996 
   Size:       3724     272
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     426    3554    3996 
   Size:        426    3128     442

  Fragments arranged by size: 

                3128     442     426
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1214    2918    3341    3653    3845    3996 
   Size:       1214    1704     423     312     192     151

  Fragments arranged by size: 

                1704    1214     423     312     192     151
 
Bpu10I CC'TnA_GC

Cuts at:      0     825    2222    3996 
   Size:        825    1397    1774

  Fragments arranged by size: 

                1774    1397     825
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      75    3996 
   Size:         75    3921
 
BsaAI yAC'GTr

Cuts at:      0    3761    3996 
   Size:       3761     235
 
BsaBI GATnn'nnATC

Cuts at:      0    3317    3509    3996 
   Size:       3317     192     487

  Fragments arranged by size: 

                3317     487     192
 
BsaHI Gr'CG_yC

Cuts at:      0      10    1185    3996 
   Size:         10    1175    2811

  Fragments arranged by size: 

                2811    1175      10
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1187    1217    1793    1823    3783    3813    3996 
   Size:       1187      30     576      30    1960      30     183

  Fragments arranged by size: 

                1960    1187     576     183      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     226    2889    2970    3428    3431    3440    3530 
   Size:         52     174    2663      81     458       3       9      90

Cuts at:   3530    3533    3773    3996 
   Size:          3     240     223

  Fragments arranged by size: 

                2663     458     240     223     174      90      81      52
                   9       3       3
 
BseYI C'CCAG_C

Cuts at:      0     185    3996 
   Size:        185    3811
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1469    2027    2642    3996 
   Size:       1469     558     615    1354

  Fragments arranged by size: 

                1469    1354     615     558
 
BsiHKAI G_wGCw'C

Cuts at:      0    1523    3830    3996 
   Size:       1523    2307     166

  Fragments arranged by size: 

                2307    1523     166
 
Bsp1286I G_dGCh'C

Cuts at:      0    1523    3428    3830    3996 
   Size:       1523    1905     402     166

  Fragments arranged by size: 

                1905    1523     402     166
 
BspHI T'CATG_A

Cuts at:      0     915    1133    1478    2321    3468    3996 
   Size:        915     218     345     843    1147     528

  Fragments arranged by size: 

                1147     915     843     528     345     218
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     954    3996 
   Size:        954    3042
 
BsrDI GCAATG_nn'

Cuts at:      0     257     971    3996 
   Size:        257     714    3025

  Fragments arranged by size: 

                3025     714     257
 
BsrGI T'GTAC_A

Cuts at:      0    3956    3996 
   Size:       3956      40
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     274    3996 
   Size:        274    3722
 
BstEII G'GTnAC_C

Cuts at:      0    3160    3996 
   Size:       3160     836
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2279    3996 
   Size:       2279    1717
 
BstYI r'GATC_y

Cuts at:      0    1197    1851    2057    3318    3627    3996 
   Size:       1197     654     206    1261     309     369

  Fragments arranged by size: 

                1261    1197     654     369     309     206
 
BstZ17I GTA'TAC

Cuts at:      0    2643    3996 
   Size:       2643    1353
 
Bsu36I CC'TnA_GG

Cuts at:      0    3144    3996 
   Size:       3144     852
 
BtgI C'CryG_G

Cuts at:      0    2981    3996 
   Size:       2981    1015
 
BtsI GCAGTG_nn'

Cuts at:      0    2668    3996 
   Size:       2668    1328
 
ClaI AT'CG_AT

Cuts at:      0    1280    3996 
   Size:       1280    2716
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1892    1927    3996 
   Size:       1892      35    2069

  Fragments arranged by size: 

                2069    1892      35
 
DraI TTT'AAA

Cuts at:      0     425     987    2730    2837    3996 
   Size:        425     562    1743     107    1159

  Fragments arranged by size: 

                1743    1159     562     425     107
 
DraIII CAC_nnn'GTG

Cuts at:      0    2770    3996 
   Size:       2770    1226
 
EaeI y'GGCC_r

Cuts at:      0     938    1419    3996 
   Size:        938     481    2577

  Fragments arranged by size: 

                2577     938     481
 
EarI CTCTTCn'nnn_

Cuts at:      0     119     140     851    1628    3416    3449    3638    3996 
   Size:        119      21     711     777    1788      33     189     358

  Fragments arranged by size: 

                1788     777     711     358     189     119      33      21
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3602    3996 
   Size:       3602     394
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15    1214    1607    1730    2827    2918    3275    3341 
   Size:         15    1199     393     123    1097      91     357      66

Cuts at:   3341    3653    3685    3845    3996 
   Size:        312      32     160     151

  Fragments arranged by size: 

                1199    1097     393     357     312     160     151     123
                  91      66      32      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      82    3122    3801    3996 
   Size:         82    3040     679     195

  Fragments arranged by size: 

                3040     679     195      82
 
EcoRI G'AATT_C

Cuts at:      0    1321    3888    3996 
   Size:       1321    2567     108

  Fragments arranged by size: 

                2567    1321     108
 
EcoRV GAT'ATC

Cuts at:      0     717    3996 
   Size:        717    3279
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     700    3402    3996 
   Size:        700    2702     594

  Fragments arranged by size: 

                2702     700     594
 
HaeII r_GCGC'y

Cuts at:      0      13    3996 
   Size:         13    3983
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      53      85     219     251     256     288    2316    2348 
   Size:         53      32     134      32       5      32    2028      32

Cuts at:   2348    3214    3246    3423    3455    3996 
   Size:        866      32     177      32     541

  Fragments arranged by size: 

                2028     866     541     177     134      53      32      32
                  32      32      32      32       5
 
HincII GTy'rAC

Cuts at:      0    1189    1832    3996 
   Size:       1189     643    2164

  Fragments arranged by size: 

                2164    1189     643
 
HindIII A'AGCT_T

Cuts at:      0    3198    3996 
   Size:       3198     798
 
HpaI GTT'AAC

Cuts at:      0    1832    3996 
   Size:       1832    2164
 
KasI G'GCGC_C

Cuts at:      0       9    3996 
   Size:          9    3987
 
KpnI G_GTAC'C

Cuts at:      0    3811    3996 
   Size:       3811     185
 
MfeI C'AATT_G

Cuts at:      0     242    1069    2189    2273    3996 
   Size:        242     827    1120      84    1723

  Fragments arranged by size: 

                1723    1120     827     242      84
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2064    3187    3996 
   Size:       2064    1123     809

  Fragments arranged by size: 

                2064    1123     809
 
MscI TGG'CCA

Cuts at:      0     940    1421    3996 
   Size:        940     481    2575

  Fragments arranged by size: 

                2575     940     481
 
MslI CAynn'nnrTG

Cuts at:      0     487     664    1526    1844    2096    3996 
   Size:        487     177     862     318     252    1900

  Fragments arranged by size: 

                1900     862     487     318     252     177
 
MspA1I CmG'CkG

Cuts at:      0     162     415     836    2355    3996 
   Size:        162     253     421    1519    1641

  Fragments arranged by size: 

                1641    1519     421     253     162
 
NarI GG'CG_CC

Cuts at:      0      10    3996 
   Size:         10    3986
 
NcoI C'CATG_G

Cuts at:      0    2981    3996 
   Size:       2981    1015
 
NdeI CA'TA_TG

Cuts at:      0    1841    3996 
   Size:       1841    2155
 
NsiI A_TGCA'T

Cuts at:      0     181    1533    2509    3996 
   Size:        181    1352     976    1487

  Fragments arranged by size: 

                1487    1352     976     181
 
NspI r_CATG'y

Cuts at:      0     525    1082    1531    3996 
   Size:        525     557     449    2465

  Fragments arranged by size: 

                2465     557     525     449
 
PciI A'CATG_T

Cuts at:      0     521    3996 
   Size:        521    3475
 
PfoI T'CCnGG_A

Cuts at:      0    3105    3996 
   Size:       3105     891
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3554    3586    3996 
   Size:       3554      32     410

  Fragments arranged by size: 

                3554     410      32
 
PsiI TTA'TAA

Cuts at:      0    3682    3996 
   Size:       3682     314
 
PstI C_TGCA'G

Cuts at:      0     951    2675    3996 
   Size:        951    1724    1321

  Fragments arranged by size: 

                1724    1321     951
 
PvuII CAG'CTG

Cuts at:      0     162     415     836    2355    3996 
   Size:        162     253     421    1519    1641

  Fragments arranged by size: 

                1641    1519     421     253     162
 
SalI G'TCGA_C

Cuts at:      0    1187    3996 
   Size:       1187    2809
 
SapI GCTCTTCn'nnn_

Cuts at:      0     140     851    3416    3996 
   Size:        140     711    2565     580

  Fragments arranged by size: 

                2565     711     580     140
 
ScaI AGT'ACT

Cuts at:      0    1638    2546    3996 
   Size:       1638     908    1450

  Fragments arranged by size: 

                1638    1450     908
 
SfcI C'TryA_G

Cuts at:      0     278     947    1808    2671    3996 
   Size:        278     669     861     863    1325

  Fragments arranged by size: 

                1325     863     861     669     278
 
SfoI GGC'GCC

Cuts at:      0      11    3996 
   Size:         11    3985
 
SmlI C'TyrA_G

Cuts at:      0    2749    3996 
   Size:       2749    1247
 
SphI G_CATG'C

Cuts at:      0    1531    3996 
   Size:       1531    2465
 
SspI AAT'ATT

Cuts at:      0    1568    2247    2802    3996 
   Size:       1568     679     555    1194

  Fragments arranged by size: 

                1568    1194     679     555
 
StuI AGG'CCT

Cuts at:      0    3117    3996 
   Size:       3117     879
 
StyI C'CwwG_G

Cuts at:      0     190    1143    2981    3076    3485    3802    3880    3996 
   Size:        190     953    1838      95     409     317      78     116

  Fragments arranged by size: 

                1838     953     409     317     190     116      95      78
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     254    1172    2827    3179    3996 
   Size:        254     918    1655     352     817

  Fragments arranged by size: 

                1655     918     817     352     254
 
TatI w'GTAC_w

Cuts at:      0     396    1636    2046    2544    3956    3996 
   Size:        396    1240     410     498    1412      40

  Fragments arranged by size: 

                1412    1240     498     410     396      40
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     952     984    1796    1828    3783    3815    3996 
   Size:        952      32     812      32    1955      32     181

  Fragments arranged by size: 

                1955     952     812     181      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1641    3996 
   Size:       1641    2355
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     248    2276    3996 
   Size:        248    2028    1720

  Fragments arranged by size: 

                2028    1720     248
 
XmnI GAAnn'nnTTC

Cuts at:      0     680    2593    3996 
   Size:        680    1913    1403

  Fragments arranged by size: 

                1913    1403     680
 
ZraI GAC'GTC

Cuts at:      0    1186    3996 
   Size:       1186    2810
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AhdI     AlfI
    AlwNI     ApoI     AseI    AvrII     BanI    BanII     BbeI     BbsI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EarI     EciI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NsiI     NspI     PciI     PfoI     PpiI
     PsiI     PstI    PvuII     SalI     SapI     ScaI     SfcI     SfoI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI     AgeI     AleI     AloI     ApaI    ApaLI
     AscI    AsiSI     AvaI     BaeI    BamHI    BbvCI     BcgI    BglII
 Bme1580I     BmtI    BpuEI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII    BssSI    BstBI    BtgZI     DrdI
     EagI  EcoICRI EcoO109I     FseI     FspI    FspAI     MluI     NaeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI     PmeI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XhoI     XmaI
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