Restriction map for pF1KB7261
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AfaI        AflII       AflIII      AgeI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacII       SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          328  GTCGAC               GT^[AC][GT]AC
AccIII        555  TCCGGA               T^CCGGA
AhdI          331  GACCCCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         331  GACCCCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         191  GAGCTC               G[AT]GC[AT]^C
AvaI          223  CCCGAG               C^[CT]CG[GA]G
BalI          455  TGGCCA               TGG^CCA
BanII         191  GAGCTC               G[GA]GC[CT]^C
BciVI          37  GGATAC               GTATCC(6/5)
BcnI          123  CCGGG                CC^[GC]GG
BsaWI         555  TCCGGA               [AT]^CCGG[AT]
BseAI         555  TCCGGA               T^CCGGA
BsgI          384  CTGCAC               GTGCAG(16/14)
BsiHKAI       191  GAGCTC               G[AT]GC[AT]^C
BsoBI         223  CCCGAG               C^[CT]CG[GA]G
BspEI         555  TCCGGA               T^CCGGA
BsrFI         388  ACCGGC               [GA]^CCGG[CT]
BssSI         402  CACGAG               CACGAG(-5/-1)
BstAPI        386  GCACCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        388  ACCGGC               [GA]^CCGG[CT]
Eam1105I      331  GACCCCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII        328  GTCGAC               GT[CT]^[GA]AC
HindII        328  GTCGAC               GT[CT]^[GA]AC
MluNI         455  TGGCCA               TGG^CCA
MroI          555  TCCGGA               T^CCGGA
MscI          455  TGGCCA               TGG^CCA
MseI          490  TTAA                 T^TAA
MslI          576  CACGGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        278  CCGCTG               C[AC]G^C[GT]G
NciI          123  CCGGG                CC^[GC]GG
NspI          216  GCATGT               [GA]CATG^[CT]
PflMI          48  CCATGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          191  GAGCTC               GAGCT^C
SalI          328  GTCGAC               G^TCGAC
Tru9I         490  TTAA                 T^TAA
Tsp509I       488  AATT                 ^AATT
TspRI         408  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I         48  CCATGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AluI          192  AGCT                 AG^CT
              270  AGCT                
AlwI           72  GATCC                GGATC(4/5)
              111  GATCC               
BmyI          191  GAGCTC               G[AGT]GC[ACT]^C
              220  GTGCCC              
BpmI          130  CTCCAG               CTGGAG(16/14)
              142  CTGGAG              
BseRI          25  CTCCTC               GAGGAG(10/8)
              514  GAGGAG              
BsmFI         125  GGGAC                GGGAC(10/14)
              274  GTCCC               
Bsp1286I      191  GAGCTC               G[AGT]GC[ACT]^C
              220  GTGCCC              
BstF5I        120  CATCC                GGATG(2/0)
              579  GGATG               
BstYI          71  AGATCC               [GA]^GATC[CT]
              110  AGATCC              
CfoI          530  GCGC                 GCG^C
              536  GCGC                
DdeI          187  CTGAG                C^T[ACGT]AG
              212  CTAAG               
DraII           4  AGGACCT              [GA]G^G[ACGT]CC[CT]
              125  GGGACCT             
EaeI           56  TGGCCG               [CT]^GGCC[GA]
              455  TGGCCA              
EarI          252  GAAGAG               CTCTTC(1/4)
              568  GAAGAG              
EcoO109I        4  AGGACCT              [GA]G^G[ACGT]CC[CT]
              125  GGGACCT             
EcoT14I         8  CCTTGG               C^C[AT][AT]GG
              458  CCAAGG              
FokI          120  CATCC                GGATG(9/13)
              579  GGATG               
HgaI          431  GCGTC                GACGC(5/10)
              446  GCGTC               
HhaI          530  GCGC                 GCG^C
              536  GCGC                
HinP1I        530  GCGC                 G^CGC
              536  GCGC                
Ksp632I       252  GAAGAG               CTCTTC(1/4)
              568  GAAGAG              
MaeII         337  ACGT                 A^CGT
              470  ACGT                
MflI           71  AGATCC               [GA]^GATC[CT]
              110  AGATCC              
PpuMI           4  AGGACCT              [GA]G^G[AT]CC[CT]
              125  GGGACCT             
SfaNI         119  GCATC                GCATC(5/9)
              260  GCATC               
StyI            8  CCTTGG               C^C[AT][AT]GG
              458  CCAAGG              
XhoII          71  AGATCC               [GA]^GATC[CT]
              110  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AvaII       BbvI        BsaJI       
BsiYI       BslI        BssKI       BstNI       BstUI       
Cac8I       Cfr13I      DpnII       EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       TaqI        TfiI        TseI        TthHB8I     
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