Restriction map for pF1KB7245
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BbeI        BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       CfoI        ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tsp509I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          100  GTCTAC               GT^[AC][GT]AC
AlwNI         224  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          864  CCTGAGG              CC^T[ACGT]AGG
AspHI          64  GAGCTC               G[AT]GC[AT]^C
AspI           31  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           59  CTCGGG               C^[CT]CG[GA]G
AvaII         249  GGACC                G^G[AT]CC
BanI          325  GGTGCC               G^G[CT][GA]CC
BbrPI          18  CACGTG               CAC^GTG
BbvCI         672  GCTGAGG              CCTCAGC(-5/-2)
BsaAI          18  CACGTG               [CT]AC^GT[GA]
BsiHKAI        64  GAGCTC               G[AT]GC[AT]^C
BsoBI          59  CTCGGG               C^[CT]CG[GA]G
BspMI         715  ACCTGC               ACCTGC(4/8)
BsrDI         369  CATTGC               GCAATG(2/0)
BsrFI         911  ACCGGC               [GA]^CCGG[CT]
Bsu36I        864  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        911  ACCGGC               [GA]^CCGG[CT]
DsaI          329  CCATGG               C^C[GA][CT]GG
EaeI          819  CGGCCA               [CT]^GGCC[GA]
EarI          219  CTCTTC               CTCTTC(1/4)
Eco81I        864  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       329  CCATGG               C^C[AT][AT]GG
HindIII       805  AAGCTT               A^AGCTT
Ksp632I       219  CTCTTC               CTCTTC(1/4)
MaeII          19  ACGT                 A^CGT
MspA1I        475  CAGCTG               C[AC]G^C[GT]G
MwoI          489  GCTCCCACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          329  CCATGG               C^CATGG
NspI          878  ACATGC               [GA]CATG^[CT]
PflFI          31  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         312  CCACTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           57  GACTC                GAGTC(4/5)
PmaCI          18  CACGTG               CAC^GTG
PmlI           18  CACGTG               CAC^GTG
PvuII         475  CAGCTG               CAG^CTG
SacI           64  GAGCTC               GAGCT^C
ScaI          549  AGTACT               AGT^ACT
SmlI          592  CTCAAG               C^T[CT][GA]AG
StyI          329  CCATGG               C^C[AT][AT]GG
TfiI          154  GATTC                G^A[AT]TC
Tth111I        31  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        312  CCACTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          236  CCAAAATGCTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI          363  CCGC                 CCGC(-3/-1)
              390  CCGC                
AfaI          177  GTAC                 GT^AC
              550  GTAC                
BanII          46  GGGCTC               G[GA]GC[CT]^C
               64  GAGCTC              
BbsI           37  GTCTTC               GAAGAC(2/6)
              334  GTCTTC              
BcnI          380  CCCGG                CC^[GC]GG
              776  CCCGG               
BfaI          205  CTAG                 C^TAG
              244  CTAG                
BglII         299  AGATCT               A^GATCT
              596  AGATCT              
BmyI           46  GGGCTC               G[AGT]GC[ACT]^C
               64  GAGCTC              
BpmI           67  CTCCAG               CTGGAG(16/14)
              583  CTGGAG              
BpuAI          37  GTCTTC               GAAGAC(2/6)
              334  GTCTTC              
BseRI         253  CTCCTC               GAGGAG(10/8)
              637  CTCCTC              
BsgI          564  CTGCAC               GTGCAG(16/14)
              717  CTGCAC              
BsmAI         555  GAGAC                GTCTC(1/5)
              906  GAGAC               
Bsp1286I       46  GGGCTC               G[AGT]GC[ACT]^C
               64  GAGCTC              
BspHI         404  TCATGA               T^CATGA
              830  TCATGA              
BstYI         299  AGATCT               [GA]^GATC[CT]
              596  AGATCT              
HinfI          57  GACTC                G^A[ACGT]TC
              154  GATTC               
MaeI          205  CTAG                 C^TAG
              244  CTAG                
MflI          299  AGATCT               [GA]^GATC[CT]
              596  AGATCT              
NciI          380  CCCGG                CC^[GC]GG
              776  CCCGG               
RcaI          404  TCATGA               T^CATGA
              830  TCATGA              
RsaI          177  GTAC                 GT^AC
              550  GTAC                
SfaNI         486  GATGC                GCATC(5/9)
              669  GATGC               
XhoII         299  AGATCT               [GA]^GATC[CT]
              596  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MslI        MspI        MvaI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TseI        
Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com