Restriction map for pF1KB7235
 (Circular) (Six-base) MAPSORT of: pF1KB7235.seq  Check: 1012  from: 1  to: 5018



pF1KB7235  5018 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:   2056    2056 
   Size:       5018
 
Acc65I G'GTAC_C

Cuts at:   1088    2034    1088 
   Size:        946    4072
 
AclI AA'CG_TT

Cuts at:   4858    4858 
   Size:       5018
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    241     541     785    1015    2843    3275    4022    4290     241 
   Size:        300     244     230    1828     432     747     268     969

  Fragments arranged by size: 

                1828     969     747     432     300     268     244     230
            
 
AflIII A'CryG_T

Cuts at:    380    2370    3474    4451     380 
   Size:       1990    1104     977     947

  Fragments arranged by size: 

                1990    1104     977     947
 
AgeI A'CCGG_T

Cuts at:   3406    3406 
   Size:       5018
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1089    1676    1089 
   Size:        587    4431
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1790    1822    1790 
   Size:         32    4986
 
AlwNI CAG_nnn'CTG

Cuts at:    303     718    2323    3890     303 
   Size:        415    1605    1567    1431

  Fragments arranged by size: 

                1605    1567    1431     415
 
ApaI G_GGCC'C

Cuts at:    123    1440     123 
   Size:       1317    3701
 
ApaLI G'TGCA_C

Cuts at:   3788    3788 
   Size:       5018
 
ApoI r'AATT_y

Cuts at:   2022    4371    4627    2022 
   Size:       2349     256    2413

  Fragments arranged by size: 

                2413    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5018
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5018
 
AvaI C'yCGr_G

Cuts at:   1221    1432    1439    2038    1221 
   Size:        211       7     599    4201

  Fragments arranged by size: 

                4201     599     211       7
 
AvrII C'CTAG_G

Cuts at:   1722    1722 
   Size:       5018
 
BamHI G'GATC_C

Cuts at:    916    1259    2043     916 
   Size:        343     784    3891

  Fragments arranged by size: 

                3891     784     343
 
BanI G'GyrC_C

Cuts at:   1088    2034    2448    2696    2731    1088 
   Size:        946     414     248      35    3375

  Fragments arranged by size: 

                3375     946     414     248      35
 
BanII G_rGCy'C

Cuts at:    123     218    1249    1440    1534    1759    2032    3062     123 
   Size:         95    1031     191      94     225     273    1030    2079

  Fragments arranged by size: 

                2079    1031    1030     273     225     191      95      94
            
 
BbeI G_GCGC'C

Cuts at:   2452    2700    2452 
   Size:        248    4770
 
BbvCI CC'TCA_GC

Cuts at:   1618    1645    1618 
   Size:         27    4991
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     365     399      90 
   Size:         34     241      34    4709

  Fragments arranged by size: 

                4709     241      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2916    3677    5018    2916 
   Size:        761    1341    2916

  Fragments arranged by size: 

                2916    1341     761
 
BclI T'GATC_A

Cuts at:   2538    2538 
   Size:       5018
 
BglII A'GATC_T

Cuts at:   1307    2533    4570    1307 
   Size:       1226    2037    1755

  Fragments arranged by size: 

                2037    1755    1226
 
BlpI GC'TnA_GC

Cuts at:    743    2130     743 
   Size:       1387    3631
 
Bme1580I G_kGCm'C

Cuts at:    123    1068    1440    1930    2643    2736    3792     123 
   Size:        945     372     490     713      93    1056    1349

  Fragments arranged by size: 

                1349    1056     945     713     490     372      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    278     509    2230    2311    2646    4790     278 
   Size:        231    1721      81     335    2144     506

  Fragments arranged by size: 

                2144    1721     506     335     231      81
 
BmtI G_CTAG'C

Cuts at:   1037    1037 
   Size:       5018
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3385    3385 
   Size:       5018
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    566     829     971    1296    1320    1441    1474    1548     566 
   Size:        263     142     325      24     121      33      74    4036

  Fragments arranged by size: 

                4036     325     263     142     121      74      33      24
            
 
Bpu10I CC'TnA_GC

Cuts at:   1618    1645    1753    1618 
   Size:         27     108    4883

  Fragments arranged by size: 

                4883     108      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2194    3073    3565    3863    2194 
   Size:        879     492     298    3349

  Fragments arranged by size: 

                3349     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1360    1552    1564    4722    1360 
   Size:        192      12    3158    1656

  Fragments arranged by size: 

                3158    1656     192      12
 
BsaAI yAC'GTr

Cuts at:    381    2371     381 
   Size:       1990    3028
 
BsaBI GATnn'nnATC

Cuts at:      6    2532    4569       6 
   Size:       2526    2037     455

  Fragments arranged by size: 

                2526    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2449    2697    3399    4895    2449 
   Size:        248     702    1496    2572

  Fragments arranged by size: 

                2572    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2207    2728    3406    3680    3827    4238    2207 
   Size:        521     678     274     147     411    2987

  Fragments arranged by size: 

                2987     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    944     974    1231    1261    1790    1820     944 
   Size:         30     257      30     529      30    4142

  Fragments arranged by size: 

                4142     529     257      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    136     586    1457     136 
   Size:        450     871    3697

  Fragments arranged by size: 

                3697     871     450
 
BseYI C'CCAG_C

Cuts at:    566    1611    1801    2443    3201    3778    4274     566 
   Size:       1045     190     642     758     577     496    1310

  Fragments arranged by size: 

                1310    1045     758     642     577     496     190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    412    1646    1696     412 
   Size:       1234      50    3734

  Fragments arranged by size: 

                3734    1234      50
 
BsiEI CG_ry'CG

Cuts at:    104    2560    2606    3814     104 
   Size:       2456      46    1208    1308

  Fragments arranged by size: 

                2456    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    744    1534    2032    2810    3000    3792     744 
   Size:        790     498     778     190     792    1970

  Fragments arranged by size: 

                1970     792     790     778     498     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    418     418 
   Size:       5018
 
Bsp1286I G_dGCh'C

Cuts at:    123     218     744    1068    1249    1440    1534    1759    1930 
   Size:         95     526     324     181     191      94     225     171

Cuts at:   1930    2032    2643    2736    2810    3000    3062    3792     123 
   Size:        102     611      93      74     190      62     730    1349

  Fragments arranged by size: 

                1349     730     611     526     324     225     191     190
                 181     171     102      95      94      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   4238    4238 
   Size:       5018
 
BspHI T'CATG_A

Cuts at:   4194    4194 
   Size:       5018
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    691     953    1718    2068    2282    2584    2965     691 
   Size:        262     765     350     214     302     381    2744

  Fragments arranged by size: 

                2744     765     381     350     302     262     214
 
BsrBI CCG'CTC

Cuts at:     50    1597    3310    3364    4848    5013      50 
   Size:       1547    1713      54    1484     165      55

  Fragments arranged by size: 

                1713    1547    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    982    1967    2930     982 
   Size:        985     963    3070

  Fragments arranged by size: 

                3070     985     963
 
BsrFI r'CCGG_y

Cuts at:    504    3016    3197    3406     504 
   Size:       2512     181     209    2116

  Fragments arranged by size: 

                2512    2116     209     181
 
BssHII G'CGCG_C

Cuts at:   1491    1491 
   Size:       5018
 
BssSI C'ACGA_G

Cuts at:   1533    3289    3647    1533 
   Size:       1756     358    2904

  Fragments arranged by size: 

                2904    1756     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1608    2395    1608 
   Size:        787    4231
 
BstBI TT'CG_AA

Cuts at:   3379    3379 
   Size:       5018
 
BstEII G'GTnAC_C

Cuts at:    132    1209    1590     132 
   Size:       1077     381    3560

  Fragments arranged by size: 

                3560    1077     381
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1352    1352 
   Size:       5018
 
BstYI r'GATC_y

Cuts at:      1     630     893     916    1259    1307    1580    2043    2510 
   Size:        629     263      23     343      48     273     463     467

Cuts at:   2510    2533    2868    3114    4126    4212    4224    4570       1 
   Size:         23     335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     629     467     463     449     346     343     335
                 273     263     246      86      48      23      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:    601    1735     601 
   Size:       1134    3884
 
BtgI C'CryG_G

Cuts at:    316     530     653    1934     316 
   Size:        214     123    1281    3400

  Fragments arranged by size: 

                3400    1281     214     123
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2968    3149    3312    2968 
   Size:        181     163    4674

  Fragments arranged by size: 

                4674     181     163
 
BtsI GCAGTG_nn'

Cuts at:    965    1742    2261     965 
   Size:        777     519    3722

  Fragments arranged by size: 

                3722     777     519
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1535    1570    1950    1985    1535 
   Size:         35     380      35    4568

  Fragments arranged by size: 

                4568     380      35      35
 
DraI TTT'AAA

Cuts at:   2017    2017 
   Size:       5018
 
DrdI GACnn_nn'nnGTC

Cuts at:   2724    3582    2724 
   Size:        858    4160
 
EaeI y'GGCC_r

Cuts at:   2603    2777    3168    3195    4540    2603 
   Size:        174     391      27    1345    3081

  Fragments arranged by size: 

                3081    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2603    2603 
   Size:       5018
 
EarI CTCTTCn'nnn_

Cuts at:    130     266     421    1106    1112    3041    3251     130 
   Size:        136     155     685       6    1929     210    1897

  Fragments arranged by size: 

                1929    1897     685     210     155     136       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3226    3536    3682    4828    3226 
   Size:        310     146    1146    3416

  Fragments arranged by size: 

                3416    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1532    2030    1532 
   Size:        498    4520
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    241     541     566     785     829     971    1015    1296    1320 
   Size:        300      25     219      44     142      44     281      24

Cuts at:   1320    1441    1474    1548    2843    3275    4022    4290     241 
   Size:        121      33      74    1295     432     747     268     969

  Fragments arranged by size: 

                1295     969     747     432     300     281     268     219
                 142     121      74      44      44      33      25      24
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1204    1833    1204 
   Size:        629    4389
 
EcoO109I rG'GnC_Cy

Cuts at:    120     246    1436    1448    1522    1719    1949    2157     120 
   Size:        126    1190      12      74     197     230     208    2981

  Fragments arranged by size: 

                2981    1190     230     208     197     126      74      12
            
 
EcoRI G'AATT_C

Cuts at:   2022    2022 
   Size:       5018
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3037    3037 
   Size:       5018
 
FspI TGC'GCA

Cuts at:    877    2799     877 
   Size:       1922    3096
 
HaeII r_GCGC'y

Cuts at:    283    1625    2452    2700    3722    4701     283 
   Size:       1342     827     248    1022     979     600

  Fragments arranged by size: 

                1342    1022     979     827     600     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    453     485     880     912    1359    1391    1933    1965    2860 
   Size:         32     395      32     447      32     542      32     895

Cuts at:   2860    2890    2892    2922     453 
   Size:         30       2      30    2549

  Fragments arranged by size: 

                2549     895     542     447     395      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   2057    2057 
   Size:       5018
 
HindIII A'AGCT_T

Cuts at:    797     797 
   Size:       5018
 
KasI G'GCGC_C

Cuts at:   2448    2696    2448 
   Size:        248    4770
 
KpnI G_GTAC'C

Cuts at:   1092    2038    1092 
   Size:        946    4072
 
MluI A'CGCG_T

Cuts at:   4451    4451 
   Size:       5018
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1520    3689    3873    1520 
   Size:       2169     184    2665

  Fragments arranged by size: 

                2665    2169     184
 
MscI TGG'CCA

Cuts at:   2779    2779 
   Size:       5018
 
MslI CAynn'nnrTG

Cuts at:   1201    3007    3134    1201 
   Size:       1806     127    3085

  Fragments arranged by size: 

                3085    1806     127
 
MspA1I CmG'CkG

Cuts at:    300     318     426     566     666     873    1488    2129    2443 
   Size:         18     108     140     100     207     615     641     314

Cuts at:   2443    2803    3816    4061     300 
   Size:        360    1013     245    1257

  Fragments arranged by size: 

                1257    1013     641     615     360     314     245     207
                 140     108     100      18
 
NaeI GCC'GGC

Cuts at:    506    3199     506 
   Size:       2693    2325
 
NarI GG'CG_CC

Cuts at:   2449    2697    2449 
   Size:        248    4770
 
NcoI C'CATG_G

Cuts at:    530    1934     530 
   Size:       1404    3614
 
NgoMIV G'CCGG_C

Cuts at:    504    3197     504 
   Size:       2693    2325
 
NheI G'CTAG_C

Cuts at:   1033    1033 
   Size:       5018
 
NspI r_CATG'y

Cuts at:    360    1687    2071    2571    3478     360 
   Size:       1327     384     500     907    1900

  Fragments arranged by size: 

                1900    1327     907     500     384
 
PciI A'CATG_T

Cuts at:   3474    3474 
   Size:       5018
 
PfoI T'CCnGG_A

Cuts at:   1131    1131 
   Size:       5018
 
PmeI GTTT'AAAC

Cuts at:   2017    2017 
   Size:       5018
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1790    1822    4157    4189    1790 
   Size:         32    2335      32    2619

  Fragments arranged by size: 

                2619    2335      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    246    1448    1522    1719    1949     246 
   Size:       1202      74     197     230    3315

  Fragments arranged by size: 

                3315    1202     230     197      74
 
PspOMI G'GGCC_C

Cuts at:    119    1436     119 
   Size:       1317    3701
 
PstI C_TGCA'G

Cuts at:    701     872    1749    2065    2750     701 
   Size:        171     877     316     685    2969

  Fragments arranged by size: 

                2969     877     685     316     171
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5018
 
PvuII CAG'CTG

Cuts at:    300     566     666     873    1488    2443    2803     300 
   Size:        266     100     207     615     955     360    2515

  Fragments arranged by size: 

                2515     955     615     360     266     207     100
 
RsrII CG'GwC_CG

Cuts at:   3213    3213 
   Size:       5018
 
SacI G_AGCT'C

Cuts at:   1534    2032    1534 
   Size:        498    4520
 
SacII CC_GC'GG

Cuts at:    319     319 
   Size:       5018
 
SalI G'TCGA_C

Cuts at:   2055    2055 
   Size:       5018
 
SanDI GG'GwC_CC

Cuts at:   1448    1448 
   Size:       5018
 
SapI GCTCTTCn'nnn_

Cuts at:   3041    3251    3041 
   Size:        210    4808
 
SbfI CC_TGCA'GG

Cuts at:   2065    2065 
   Size:       5018
 
ScaI AGT'ACT

Cuts at:   4535    4535 
   Size:       5018
 
SfcI C'TryA_G

Cuts at:     33     697     868    1138    1668    1745    2061    2746    3739 
   Size:        664     171     270     530      77     316     685     993

Cuts at:   3739    3930      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     685     664     530     316     270     191
                 171      77
 
SfoI GGC'GCC

Cuts at:   2450    2698    2450 
   Size:        248    4770
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5018
 
SmaI CCC'GGG

Cuts at:   1223    2040    1223 
   Size:        817    4201
 
SmlI C'TyrA_G

Cuts at:   2173    3088    3580    3842    2173 
   Size:        915     492     262    3349

  Fragments arranged by size: 

                3349     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5018
 
SphI G_CATG'C

Cuts at:    360    1687    2071    2571     360 
   Size:       1327     384     500    2807

  Fragments arranged by size: 

                2807    1327     500     384
 
StuI AGG'CCT

Cuts at:    500     705     500 
   Size:        205    4813
 
StyI C'CwwG_G

Cuts at:    530     986    1041    1518    1586    1722    1763    1934    2152 
   Size:        456      55     477      68     136      41     171     218

Cuts at:   2152    2506     530 
   Size:        354    3042

  Fragments arranged by size: 

                3042     477     456     354     218     171     136      68
                  55      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    426     523    1358    1947    1978    2601    2734    3193    3402 
   Size:         97     835     589      31     623     133     459     209

Cuts at:   3402     426 
   Size:       2042

  Fragments arranged by size: 

                2042     835     623     589     459     209     133      97
                  31
 
TatI w'GTAC_w

Cuts at:   4533    4533 
   Size:       5018
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    944     976    1318    1333    1350    1365    4535    4567     944 
   Size:         32     342      15      17      15    3170      32    1395

  Fragments arranged by size: 

                3170    1395     342      32      32      17      15      15
            
 
Tth111I GACn'n_nGTC

Cuts at:   2815    2815 
   Size:       5018
 
XbaI T'CTAG_A

Cuts at:   2049    2049 
   Size:       5018
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1295    1320    1997    2513    1295 
   Size:         25     677     516    3800

  Fragments arranged by size: 

                3800     677     516      25
 
XmaI C'CCGG_G

Cuts at:   1221    2038    1221 
   Size:        817    4201
 
XmnI GAAnn'nnTTC

Cuts at:    226     448     226 
   Size:        222    4796
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PciI     PfoI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AscI     BaeI
     BbsI    BfrBI     BglI    BmgBI    BsiWI     BsmI    BsrGI  BstZ17I
     ClaI   DraIII    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI    PshAI
     PsiI    PspXI     PsrI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     ZraI
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