Restriction map for pF1KB7235
 (Linear) (Six-base) MAPSORT of: KIBB7235.seq  Check: 267  from: 1  to: 1905



KIBB7235.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

Acc65I G'GTAC_C

Cuts at:      0     980    1905 
   Size:        980     925
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     433     677     907    1905 
   Size:        133     300     244     230     998

  Fragments arranged by size: 

                 998     300     244     230     133
 
AflIII A'CryG_T

Cuts at:      0     272    1905 
   Size:        272    1633
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     981    1568    1905 
   Size:        981     587     337

  Fragments arranged by size: 

                 981     587     337
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1682    1714    1905 
   Size:       1682      32     191

  Fragments arranged by size: 

                1682     191      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     195     610    1905 
   Size:        195     415    1295

  Fragments arranged by size: 

                1295     415     195
 
ApaI G_GGCC'C

Cuts at:      0      15    1332    1905 
   Size:         15    1317     573

  Fragments arranged by size: 

                1317     573      15
 
AvaI C'yCGr_G

Cuts at:      0    1113    1324    1331    1905 
   Size:       1113     211       7     574

  Fragments arranged by size: 

                1113     574     211       7
 
AvrII C'CTAG_G

Cuts at:      0    1614    1905 
   Size:       1614     291
 
BamHI G'GATC_C

Cuts at:      0     808    1151    1905 
   Size:        808     343     754

  Fragments arranged by size: 

                 808     754     343
 
BanI G'GyrC_C

Cuts at:      0     980    1905 
   Size:        980     925
 
BanII G_rGCy'C

Cuts at:      0      15     110    1141    1332    1426    1651    1905 
   Size:         15      95    1031     191      94     225     254

  Fragments arranged by size: 

                1031     254     225     191      95      94      15
 
BbvCI CC'TCA_GC

Cuts at:      0    1510    1537    1905 
   Size:       1510      27     368

  Fragments arranged by size: 

                1510     368      27
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     257     291    1905 
   Size:        257      34    1614

  Fragments arranged by size: 

                1614     257      34
 
BglII A'GATC_T

Cuts at:      0    1199    1905 
   Size:       1199     706
 
BlpI GC'TnA_GC

Cuts at:      0     635    1905 
   Size:        635    1270
 
Bme1580I G_kGCm'C

Cuts at:      0      15     960    1332    1822    1905 
   Size:         15     945     372     490      83

  Fragments arranged by size: 

                 945     490     372      83      15
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     170     401    1905 
   Size:        170     231    1504

  Fragments arranged by size: 

                1504     231     170
 
BmtI G_CTAG'C

Cuts at:      0     929    1905 
   Size:        929     976
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     721     863    1188    1212    1333    1366    1440 
   Size:        458     263     142     325      24     121      33      74

Cuts at:   1440    1905 
   Size:        465

  Fragments arranged by size: 

                 465     458     325     263     142     121      74      33
                  24
 
Bpu10I CC'TnA_GC

Cuts at:      0    1510    1537    1645    1905 
   Size:       1510      27     108     260

  Fragments arranged by size: 

                1510     260     108      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1252    1444    1456    1905 
   Size:       1252     192      12     449

  Fragments arranged by size: 

                1252     449     192      12
 
BsaAI yAC'GTr

Cuts at:      0     273    1905 
   Size:        273    1632
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     836     866    1123    1153    1682    1712    1905 
   Size:        836      30     257      30     529      30     193

  Fragments arranged by size: 

                 836     529     257     193      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28     478    1349    1905 
   Size:         28     450     871     556

  Fragments arranged by size: 

                 871     556     450      28
 
BseYI C'CCAG_C

Cuts at:      0     458    1503    1693    1905 
   Size:        458    1045     190     212

  Fragments arranged by size: 

                1045     458     212     190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1538    1588    1905 
   Size:        304    1234      50     317

  Fragments arranged by size: 

                1234     317     304      50
 
BsiHKAI G_wGCw'C

Cuts at:      0     636    1426    1905 
   Size:        636     790     479

  Fragments arranged by size: 

                 790     636     479
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     310    1905 
   Size:        310    1595
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     110     636     960    1141    1332    1426    1651 
   Size:         15      95     526     324     181     191      94     225

Cuts at:   1651    1822    1905 
   Size:        171      83

  Fragments arranged by size: 

                 526     324     225     191     181     171      95      94
                  83      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     583     845    1610    1905 
   Size:        583     262     765     295

  Fragments arranged by size: 

                 765     583     295     262
 
BsrBI CCG'CTC

Cuts at:      0    1489    1905 
   Size:       1489     416
 
BsrDI GCAATG_nn'

Cuts at:      0     874    1859    1905 
   Size:        874     985      46

  Fragments arranged by size: 

                 985     874      46
 
BsrFI r'CCGG_y

Cuts at:      0     396    1905 
   Size:        396    1509
 
BssHII G'CGCG_C

Cuts at:      0    1383    1905 
   Size:       1383     522
 
BssSI C'ACGA_G

Cuts at:      0    1425    1905 
   Size:       1425     480
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1500    1905 
   Size:       1500     405
 
BstEII G'GTnAC_C

Cuts at:      0      24    1101    1482    1905 
   Size:         24    1077     381     423

  Fragments arranged by size: 

                1077     423     381      24
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1244    1905 
   Size:       1244     661
 
BstYI r'GATC_y

Cuts at:      0     522     785     808    1151    1199    1472    1905 
   Size:        522     263      23     343      48     273     433

  Fragments arranged by size: 

                 522     433     343     273     263      48      23
 
Bsu36I CC'TnA_GG

Cuts at:      0     493    1627    1905 
   Size:        493    1134     278

  Fragments arranged by size: 

                1134     493     278
 
BtgI C'CryG_G

Cuts at:      0     208     422     545    1826    1905 
   Size:        208     214     123    1281      79

  Fragments arranged by size: 

                1281     214     208     123      79
 
BtsI GCAGTG_nn'

Cuts at:      0     857    1634    1905 
   Size:        857     777     271

  Fragments arranged by size: 

                 857     777     271
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1427    1462    1842    1877    1905 
   Size:       1427      35     380      35      28

  Fragments arranged by size: 

                1427     380      35      35      28
 
EarI CTCTTCn'nnn_

Cuts at:      0      22     158     313     998    1004    1905 
   Size:         22     136     155     685       6     901

  Fragments arranged by size: 

                 901     685     155     136      22       6
 
EcoICRI GAG'CTC

Cuts at:      0    1424    1905 
   Size:       1424     481
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     433     458     677     721     863     907    1188 
   Size:        133     300      25     219      44     142      44     281

Cuts at:   1188    1212    1333    1366    1440    1905 
   Size:         24     121      33      74     465

  Fragments arranged by size: 

                 465     300     281     219     142     133     121      74
                  44      44      33      25      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1096    1725    1905 
   Size:       1096     629     180

  Fragments arranged by size: 

                1096     629     180
 
EcoO109I rG'GnC_Cy

Cuts at:      0      12     138    1328    1340    1414    1611    1841    1905 
   Size:         12     126    1190      12      74     197     230      64

  Fragments arranged by size: 

                1190     230     197     126      74      64      12      12
            
 
FspI TGC'GCA

Cuts at:      0     769    1905 
   Size:        769    1136
 
HaeII r_GCGC'y

Cuts at:      0     175    1517    1905 
   Size:        175    1342     388

  Fragments arranged by size: 

                1342     388     175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     345     377     772     804    1251    1283    1825    1857 
   Size:        345      32     395      32     447      32     542      32

Cuts at:   1857    1905 
   Size:         48

  Fragments arranged by size: 

                 542     447     395     345      48      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     689    1905 
   Size:        689    1216
 
KpnI G_GTAC'C

Cuts at:      0     984    1905 
   Size:        984     921
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1412    1905 
   Size:       1412     493
 
MslI CAynn'nnrTG

Cuts at:      0    1093    1905 
   Size:       1093     812
 
MspA1I CmG'CkG

Cuts at:      0     192     210     318     458     558     765    1380    1905 
   Size:        192      18     108     140     100     207     615     525

  Fragments arranged by size: 

                 615     525     207     192     140     108     100      18
            
 
NaeI GCC'GGC

Cuts at:      0     398    1905 
   Size:        398    1507
 
NcoI C'CATG_G

Cuts at:      0     422    1826    1905 
   Size:        422    1404      79

  Fragments arranged by size: 

                1404     422      79
 
NgoMIV G'CCGG_C

Cuts at:      0     396    1905 
   Size:        396    1509
 
NheI G'CTAG_C

Cuts at:      0     925    1905 
   Size:        925     980
 
NspI r_CATG'y

Cuts at:      0     252    1579    1905 
   Size:        252    1327     326

  Fragments arranged by size: 

                1327     326     252
 
PfoI T'CCnGG_A

Cuts at:      0    1023    1905 
   Size:       1023     882
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1682    1714    1905 
   Size:       1682      32     191

  Fragments arranged by size: 

                1682     191      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     138    1340    1414    1611    1841    1905 
   Size:        138    1202      74     197     230      64

  Fragments arranged by size: 

                1202     230     197     138      74      64
 
PspOMI G'GGCC_C

Cuts at:      0      11    1328    1905 
   Size:         11    1317     577

  Fragments arranged by size: 

                1317     577      11
 
PstI C_TGCA'G

Cuts at:      0     593     764    1641    1905 
   Size:        593     171     877     264

  Fragments arranged by size: 

                 877     593     264     171
 
PvuII CAG'CTG

Cuts at:      0     192     458     558     765    1380    1905 
   Size:        192     266     100     207     615     525

  Fragments arranged by size: 

                 615     525     266     207     192     100
 
SacI G_AGCT'C

Cuts at:      0    1426    1905 
   Size:       1426     479
 
SacII CC_GC'GG

Cuts at:      0     211    1905 
   Size:        211    1694
 
SanDI GG'GwC_CC

Cuts at:      0    1340    1905 
   Size:       1340     565
 
SfcI C'TryA_G

Cuts at:      0     589     760    1030    1560    1637    1905 
   Size:        589     171     270     530      77     268

  Fragments arranged by size: 

                 589     530     270     268     171      77
 
SmaI CCC'GGG

Cuts at:      0    1115    1905 
   Size:       1115     790
 
SphI G_CATG'C

Cuts at:      0     252    1579    1905 
   Size:        252    1327     326

  Fragments arranged by size: 

                1327     326     252
 
StuI AGG'CCT

Cuts at:      0     392     597    1905 
   Size:        392     205    1308

  Fragments arranged by size: 

                1308     392     205
 
StyI C'CwwG_G

Cuts at:      0     422     878     933    1410    1478    1614    1655    1826 
   Size:        422     456      55     477      68     136      41     171

Cuts at:   1826    1905 
   Size:         79

  Fragments arranged by size: 

                 477     456     422     171     136      79      68      55
                  41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     318     415    1250    1839    1870    1905 
   Size:        318      97     835     589      31      35

  Fragments arranged by size: 

                 835     589     318      97      35      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     836     868    1210    1225    1242    1257    1905 
   Size:        836      32     342      15      17      15     648

  Fragments arranged by size: 

                 836     648     342      32      17      15      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1187    1212    1889    1905 
   Size:       1187      25     677      16

  Fragments arranged by size: 

                1187     677      25      16
 
XmaI C'CCGG_G

Cuts at:      0    1113    1905 
   Size:       1113     792
 
XmnI GAAnn'nnTTC

Cuts at:      0     118     340    1905 
   Size:        118     222    1565

  Fragments arranged by size: 

                1565     222     118
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI     ApaI     AvaI
    AvrII    BamHI     BanI    BanII    BbvCI     BcgI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI    CspCI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FspI    HaeII    Hin4I  HindIII     KpnI     MmeI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SfcI
     SmaI     SphI     StuI     StyI    TaqII     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI     BbeI
     BbsI    BciVI     BclI    BfrBI     BglI    BmgBI     BplI    BpuEI
    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrGI    BstBI  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoRI    EcoRV     FalI     FseI    FspAI
   HincII     HpaI     KasI     MfeI     MluI     MscI     NarI     NdeI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XhoI     ZraI
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