Restriction map for pF1KB7212
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
MaeI        MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SalI        SapI        SbfI        SexAI       
SfcI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          262  GTCTAC               GT^[AC][GT]AC
AfaI           64  GTAC                 GT^AC
AlwI          198  GATCC                GGATC(4/5)
Asp700I       177  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         223  GAGCAC               G[AT]GC[AT]^C
BanI          107  GGCGCC               G^G[CT][GA]CC
BbeI          107  GGCGCC               GGCGC^C
BcnI          201  CCGGG                CC^[GC]GG
BglI          119  GCCGCGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           46  GCTAAGC              GC^T[ACGT]AGC
BmyI          223  GAGCAC               G[AGT]GC[ACT]^C
BpmI          187  CTGGAG               CTGGAG(16/14)
Bpu1102I       46  GCTAAGC              GC^T[ACGT]AGC
BseRI         157  GAGGAG               GAGGAG(10/8)
BsiHKAI       223  GAGCAC               G[AT]GC[AT]^C
BsmAI         160  GAGAC                GTCTC(1/5)
BsmFI         203  GGGAC                GGGAC(10/14)
Bsp1286I      223  GAGCAC               G[AGT]GC[ACT]^C
BsrFI          96  GCCGGC               [GA]^CCGG[CT]
BstEII        243  GGTCACC              G^GT[ACGT]ACC
BstPI         243  GGTCACC              G^GT[ACGT]ACC
BstXI         251  CCATGGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII          46  GCTAAGC              GC^T[ACGT]AGC
Cfr10I         96  GCCGGC               [GA]^CCGG[CT]
Cfr13I        282  GGGCC                G^G[ACGT]CC
DdeI           47  CTAAG                C^T[ACGT]AG
DpnII         198  GATC                 ^GATC
EcoO65I       243  GGTCACC              G^GT[ACGT]ACC
EcoT14I       251  CCATGG               C^C[AT][AT]GG
HgaI          205  GACGC                GACGC(5/10)
HinfI         162  GACTC                G^A[ACGT]TC
KasI          107  GGCGCC               G^GCGCC
MaeII         194  ACGT                 A^CGT
MboI          198  GATC                 ^GATC
NaeI           96  GCCGGC               GCC^GGC
NarI          107  GGCGCC               GG^CGCC
NciI          201  CCGGG                CC^[GC]GG
NcoI          251  CCATGG               C^CATGG
NdeII         198  GATC                 ^GATC
NgoMIV         96  GCCGGC               G^CCGGC
NlaIII        252  CATG                 CATG^
NlaIV         107  GGCGCC               GG[ACGT]^[ACGT]CC
PleI          162  GACTC                GAGTC(4/5)
RsaI           64  GTAC                 GT^AC
Sau3AI        198  GATC                 ^GATC
Sau96I        282  GGGCC                G^G[ACGT]CC
ScaI           63  AGTACT               AGT^ACT
SfoI          107  GGCGCC               GGC^GCC
SmlI          271  CTCAAG               C^T[CT][GA]AG
StyI          251  CCATGG               C^C[AT][AT]GG
XmnI          177  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI          107  GGCGCC               G[GA]^CG[CT]C
              205  GACGCC              
BsaHI         107  GGCGCC               G[GA]^CG[CT]C
              205  GACGCC              
BstF5I        102  CATCC                GGATG(2/0)
              255  GGATG               
EaeI            6  TGGCCG               [CT]^GGCC[GA]
               98  CGGCCA              
FauI           20  GCGGG                CCCGC(4/6)
              167  GCGGG               
FokI          102  CATCC                GGATG(9/13)
              255  GGATG               
Hin1I         107  GGCGCC               G[GA]^CG[CT]C
              205  GACGCC              
HphI           89  TCACC                GGTGA(8/7)
              245  TCACC               
KspI            9  CCGCGG               CCGC^GG
              120  CCGCGG              
MaeIII        211  GTGAC                ^GT[ACGT]AC
              244  GTCAC               
MslI          246  CACCGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              252  CATGGATGTG          
MspA1I          9  CCGCGG               C[AC]G^C[GT]G
              120  CCGCGG              
SacII           9  CCGCGG               CCGC^GG
              120  CCGCGG              
SfaNI          86  GCATC                GCATC(5/9)
              137  GCATC               
Tsp45I        211  GTGAC                ^GT[GC]AC
              244  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AccII       AciI        BsaJI       BssKI       BstNI       
BstUI       Cac8I       CfoI        DsaI        EcoRII      
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MnlI        MspI        
MvaI        MvnI        MwoI        ScrFI       
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