Restriction map for pF1KB7200
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeIII      MamI        MfeI        
MluI        MseI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     XbaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccIII        923  TCCGGA               T^CCGGA
AfaI          715  GTAC                 GT^AC
AlwI          455  GGATC                GGATC(4/5)
Asp700I       615  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          464  TGGCCA               TGG^CCA
BclI          383  TGATCA               T^GATCA
BfaI          226  CTAG                 C^TAG
BsaWI         923  TCCGGA               [AT]^CCGG[AT]
BseAI         923  TCCGGA               T^CCGGA
BsmFI         874  GTCCC                GGGAC(10/14)
BspEI         923  TCCGGA               T^CCGGA
BsrFI         492  GCCGGC               [GA]^CCGG[CT]
BstAPI        732  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         622  TTCGAA               TT^CGAA
BstXI         774  CCACCTGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        492  GCCGGC               [GA]^CCGG[CT]
EaeI          464  TGGCCA               [CT]^GGCC[GA]
FbaI          383  TGATCA               T^GATCA
HinfI        1167  GAGTC                G^A[ACGT]TC
HphI         1019  TCACC                GGTGA(8/7)
MaeI          226  CTAG                 C^TAG
MaeII         461  ACGT                 A^CGT
MluNI         464  TGGCCA               TGG^CCA
MroI          923  TCCGGA               T^CCGGA
MscI          464  TGGCCA               TGG^CCA
MslI         1101  CACACGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          492  GCCGGC               GCC^GGC
NcoI          945  CCATGG               C^CATGG
NgoMIV        492  GCCGGC               G^CCGGC
NspV          622  TTCGAA               TT^CGAA
PleI         1167  GAGTC                GAGTC(4/5)
RsaI          715  GTAC                 GT^AC
SacI          564  GAGCTC               GAGCT^C
SapI          625  GAAGAGC              GCTCTTC(1/4)
SfuI          622  TTCGAA               TT^CGAA
StuI           13  AGGCCT               AGG^CCT
XmnI          615  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Alw44I        358  GTGCAC               G^TGCAC
              524  GTGCAC              
AlwNI         718  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              730  CAGCAGCTG           
ApaLI         358  GTGCAC               G^TGCAC
              524  GTGCAC              
AvaI          113  CCCGGG               C^[CT]CG[GA]G
              893  CCCGGG              
BanI          237  GGCACC               G^G[CT][GA]CC
              929  GGCACC              
BbsI          418  GTCTTC               GAAGAC(2/6)
              619  GTCTTC              
BglI          763  GCCATGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              772  GCCCACCTGGC         
BglII         144  AGATCT               A^GATCT
             1085  AGATCT              
BpuAI         418  GTCTTC               GAAGAC(2/6)
              619  GTCTTC              
BsmAI         695  GTCTC                GTCTC(1/5)
              720  GTCTC               
BsoBI         113  CCCGGG               C^[CT]CG[GA]G
              893  CCCGGG              
BstYI         144  AGATCT               [GA]^GATC[CT]
             1085  AGATCT              
DsaI          945  CCATGG               C^C[GA][CT]GG
             1220  CCGTGG              
EarI          171  CTCTTC               CTCTTC(1/4)
              625  GAAGAG              
FauI          155  GCGGG                CCCGC(4/6)
             1188  GCGGG               
Ksp632I       171  CTCTTC               CTCTTC(1/4)
              625  GAAGAG              
MflI          144  AGATCT               [GA]^GATC[CT]
             1085  AGATCT              
NspI         1004  ACATGC               [GA]CATG^[CT]
             1106  GCATGT              
PflMI         663  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              975  CCACAACATGG         
PstI          205  CTGCAG               CTGCA^G
              352  CTGCAG              
SfcI          205  CTGCAG               C^T[GA][CT]AG
              352  CTGCAG              
SmaI          113  CCCGGG               CCC^GGG
              893  CCCGGG              
SmlI          427  CTCAAG               C^T[CT][GA]AG
              643  CTCAAG              
TaqI          150  TCGA                 T^CGA
              623  TCGA                
TthHB8I       150  TCGA                 T^CGA
              623  TCGA                
Van91I        663  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              975  CCACAACATGG         
XhoII         144  AGATCT               [GA]^GATC[CT]
             1085  AGATCT              
XmaCI         113  CCCGGG               C^CCGGG
              893  CCCGGG              
XmaI          113  CCCGGG               C^CCGGG
              893  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        ApaI        AspHI       AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        TspRI       
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