Restriction map for pF1KB7200
 (Linear) (Six-base) MAPSORT of: KIBB7200.seq  Check: 7096  from: 1  to: 1293



KIBB7200.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     345    1256    1293 
   Size:        345     911      37

  Fragments arranged by size: 

                 911     345      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1104    1293 
   Size:       1104     189
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     519    1293 
   Size:        519     774
 
AlwNI CAG_nnn'CTG

Cuts at:      0     723     735    1293 
   Size:        723      12     558

  Fragments arranged by size: 

                 723     558      12
 
ApaI G_GGCC'C

Cuts at:      0     114     121     162     262    1293 
   Size:        114       7      41     100    1031

  Fragments arranged by size: 

                1031     114     100      41       7
 
ApaLI G'TGCA_C

Cuts at:      0     358     524    1293 
   Size:        358     166     769

  Fragments arranged by size: 

                 769     358     166
 
AvaI C'yCGr_G

Cuts at:      0     113     893    1293 
   Size:        113     780     400

  Fragments arranged by size: 

                 780     400     113
 
BanI G'GyrC_C

Cuts at:      0     237     929    1293 
   Size:        237     692     364

  Fragments arranged by size: 

                 692     364     237
 
BanII G_rGCy'C

Cuts at:      0     114     121     162     262     376     568     835    1293 
   Size:        114       7      41     100     114     192     267     458

  Fragments arranged by size: 

                 458     267     192     114     114     100      41       7
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     411     612    1293 
   Size:        411     201     681

  Fragments arranged by size: 

                 681     411     201
 
BclI T'GATC_A

Cuts at:      0     383    1293 
   Size:        383     910
 
BglI GCCn_nnn'nGGC

Cuts at:      0     769     778    1293 
   Size:        769       9     515

  Fragments arranged by size: 

                 769     515       9
 
BglII A'GATC_T

Cuts at:      0     144    1085    1293 
   Size:        144     941     208

  Fragments arranged by size: 

                 941     208     144
 
Bme1580I G_kGCm'C

Cuts at:      0     114     121     162     252     262     362     528     560 
   Size:        114       7      41      90      10     100     166      32

Cuts at:    560     690    1293 
   Size:        130     603

  Fragments arranged by size: 

                 603     166     130     114     100      90      41      32
                  10       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     422     515    1293 
   Size:        422      93     778

  Fragments arranged by size: 

                 778     422      93
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     271     388     685     745     763    1043    1072 
   Size:         96     175     117     297      60      18     280      29

Cuts at:   1072    1252    1293 
   Size:        180      41

  Fragments arranged by size: 

                 297     280     180     175     117      96      60      41
                  29      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     628    1293 
   Size:        412     216     665

  Fragments arranged by size: 

                 665     412     216
 
BsaWI w'CCGG_w

Cuts at:      0     923    1293 
   Size:        923     370
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     266     296     680     710     817     847    1293 
   Size:        266      30     384      30     107      30     446

  Fragments arranged by size: 

                 446     384     266     107      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     159     274     688    1293 
   Size:        159     115     414     605

  Fragments arranged by size: 

                 605     414     159     115
 
BseYI C'CCAG_C

Cuts at:      0      70     105     255     375     781     876    1040    1261 
   Size:         70      35     150     120     406      95     164     221

Cuts at:   1261    1293 
   Size:         32

  Fragments arranged by size: 

                 406     221     164     150     120      95      70      35
                  32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     489    1156    1293 
   Size:         68     421     667     137

  Fragments arranged by size: 

                 667     421     137      68
 
BsiHKAI G_wGCw'C

Cuts at:      0     362     528     568    1293 
   Size:        362     166      40     725

  Fragments arranged by size: 

                 725     362     166      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     121     162     252     262     362     376     528 
   Size:        114       7      41      90      10     100      14     152

Cuts at:    528     560     568     690     835    1293 
   Size:         32       8     122     145     458

  Fragments arranged by size: 

                 458     152     145     122     114     100      90      41
                  32      14      10       8       7
 
BspEI T'CCGG_A

Cuts at:      0     923    1293 
   Size:        923     370
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     110     345    1256    1293 
   Size:        110     235     911      37

  Fragments arranged by size: 

                 911     235     110      37
 
BsrFI r'CCGG_y

Cuts at:      0     492    1293 
   Size:        492     801
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     738    1293 
   Size:        738     555
 
BstBI TT'CG_AA

Cuts at:      0     623    1293 
   Size:        623     670
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     781    1293 
   Size:        781     512
 
BstYI r'GATC_y

Cuts at:      0     144    1085    1293 
   Size:        144     941     208

  Fragments arranged by size: 

                 941     208     144
 
BtgI C'CryG_G

Cuts at:      0     945    1220    1293 
   Size:        945     275      73

  Fragments arranged by size: 

                 945     275      73
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     182    1293 
   Size:        182    1111
 
BtsI GCAGTG_nn'

Cuts at:      0     217     496     574     648    1293 
   Size:        217     279      78      74     645

  Fragments arranged by size: 

                 645     279     217      78      74
 
EaeI y'GGCC_r

Cuts at:      0     464    1293 
   Size:        464     829
 
EarI CTCTTCn'nnn_

Cuts at:      0     177     620    1293 
   Size:        177     443     673

  Fragments arranged by size: 

                 673     443     177
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     673    1144    1293 
   Size:        673     471     149

  Fragments arranged by size: 

                 673     471     149
 
EcoICRI GAG'CTC

Cuts at:      0     566    1293 
   Size:        566     727
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     271     388     685     745     763    1043    1072 
   Size:         96     175     117     297      60      18     280      29

Cuts at:   1072    1104    1252    1293 
   Size:         32     148      41

  Fragments arranged by size: 

                 297     280     175     148     117      96      60      41
                  32      29      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     110     117     158     259     865     900    1293 
   Size:        110       7      41     101     606      35     393

  Fragments arranged by size: 

                 606     393     110     101      41      35       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     817     849    1293 
   Size:        817      32     444

  Fragments arranged by size: 

                 817     444      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     848    1293 
   Size:        848     445
 
MscI TGG'CCA

Cuts at:      0     466    1293 
   Size:        466     827
 
MslI CAynn'nnrTG

Cuts at:      0    1105    1293 
   Size:       1105     188
 
MspA1I CmG'CkG

Cuts at:      0      74     204     210     735    1040    1071    1293 
   Size:         74     130       6     525     305      31     222

  Fragments arranged by size: 

                 525     305     222     130      74      31       6
 
NaeI GCC'GGC

Cuts at:      0     494    1293 
   Size:        494     799
 
NcoI C'CATG_G

Cuts at:      0     945    1293 
   Size:        945     348
 
NgoMIV G'CCGG_C

Cuts at:      0     492    1293 
   Size:        492     801
 
NspI r_CATG'y

Cuts at:      0    1008    1110    1293 
   Size:       1008     102     183

  Fragments arranged by size: 

                1008     183     102
 
PasI CC'CwG_GG

Cuts at:      0     121    1293 
   Size:        121    1172
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     669     981    1293 
   Size:        669     312     312

  Fragments arranged by size: 

                 669     312     312
 
PfoI T'CCnGG_A

Cuts at:      0     287    1049    1293 
   Size:        287     762     244

  Fragments arranged by size: 

                 762     287     244
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1219    1251    1293 
   Size:       1219      32      42

  Fragments arranged by size: 

                1219      42      32
 
PspOMI G'GGCC_C

Cuts at:      0     110     117     158     258    1293 
   Size:        110       7      41     100    1035

  Fragments arranged by size: 

                1035     110     100      41       7
 
PstI C_TGCA'G

Cuts at:      0     209     356    1293 
   Size:        209     147     937

  Fragments arranged by size: 

                 937     209     147
 
PvuII CAG'CTG

Cuts at:      0      74     204     210     735    1293 
   Size:         74     130       6     525     558

  Fragments arranged by size: 

                 558     525     130      74       6
 
SacI G_AGCT'C

Cuts at:      0     568    1293 
   Size:        568     725
 
SapI GCTCTTCn'nnn_

Cuts at:      0     620    1293 
   Size:        620     673
 
SfcI C'TryA_G

Cuts at:      0     205     352    1293 
   Size:        205     147     941

  Fragments arranged by size: 

                 941     205     147
 
SmaI CCC'GGG

Cuts at:      0     115     895    1293 
   Size:        115     780     398

  Fragments arranged by size: 

                 780     398     115
 
SmlI C'TyrA_G

Cuts at:      0     427     643    1293 
   Size:        427     216     650

  Fragments arranged by size: 

                 650     427     216
 
StuI AGG'CCT

Cuts at:      0      15    1293 
   Size:         15    1278
 
StyI C'CwwG_G

Cuts at:      0      38      45     800     861     945    1293 
   Size:         38       7     755      61      84     348

  Fragments arranged by size: 

                 755     348      84      61      38       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     102     414     468     543    1293 
   Size:        102     312      54      75     750

  Fragments arranged by size: 

                 750     312     102      75      54
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     680     712    1293 
   Size:        680      32     581

  Fragments arranged by size: 

                 680     581      32
 
XmaI C'CCGG_G

Cuts at:      0     113     893    1293 
   Size:        113     780     400

  Fragments arranged by size: 

                 780     400     113
 
XmnI GAAnn'nnTTC

Cuts at:      0     619    1293 
   Size:        619     674
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI
    BanII     BbsI     BclI     BglI    BglII Bme1580I     BmrI     BpmI
    BpuEI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrFI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI     PpiI   PspOMI     PstI    PvuII
     SacI     SapI     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TstI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BlpI
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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