Restriction map for pF1KB7197
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BamHI       
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaI        BseAI       BseRI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        Eam1105I    EcoNI       
EcoRI       EcoRV       EcoT22I     FseI        HindIII     
HinfI       HpaI        KpnI        KspI        MfeI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AocI          527  CCTCAGG              CC^T[ACGT]AGG
AvaI          572  CCCGGG               C^[CT]CG[GA]G
AviII         719  TGCGCA               TGC^GCA
BalI          290  TGGCCA               TGG^CCA
BanII         741  GGGCTC               G[GA]GC[CT]^C
BbsI          240  GAAGAC               GAAGAC(2/6)
BclI          500  TGATCA               T^GATCA
BfaI           71  CTAG                 C^TAG
BglII         641  AGATCT               A^GATCT
BpmI          598  CTCCAG               CTGGAG(16/14)
BpuAI         240  GAAGAC               GAAGAC(2/6)
BsaBI         501  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         224  TCCGGT               [AT]^CCGG[AT]
BsmAI         334  GAGAC                GTCTC(1/5)
BsmBI         334  GAGACG               CGTCTC(1/5)
BsoBI         572  CCCGGG               C^[CT]CG[GA]G
BsrBI          40  CCGCTC               CCGCTC(-3/-3)
BssHII        116  GCGCGC               G^CGCGC
BstAPI       1001  GCAAGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        633  GGTGACC              G^GT[ACGT]ACC
BstPI         633  GGTGACC              G^GT[ACGT]ACC
BstYI         641  AGATCT               [GA]^GATC[CT]
Bsu36I        527  CCTCAGG              CC^T[ACGT]AGG
DraIII        687  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        527  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       633  GGTGACC              G^GT[ACGT]ACC
FbaI          500  TGATCA               T^GATCA
FspI          719  TGCGCA               TGC^GCA
HincII        430  GTTGAC               GT[CT]^[GA]AC
HindII        430  GTTGAC               GT[CT]^[GA]AC
MaeI           71  CTAG                 C^TAG
MamI          501  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          641  AGATCT               [GA]^GATC[CT]
MluNI         290  TGGCCA               TGG^CCA
MscI          290  TGGCCA               TGG^CCA
NaeI           19  GCCGGC               GCC^GGC
NgoMIV         19  GCCGGC               G^CCGGC
NotI         1010  GCGGCCGC             GC^GGCCGC
NspI          593  GCATGC               [GA]CATG^[CT]
PpuMI         518  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI           31  CTGCAG               CTGCA^G
PvuII         615  CAGCTG               CAG^CTG
SapI           42  GCTCTTC              GCTCTTC(1/4)
SfcI           31  CTGCAG               C^T[GA][CT]AG
SgrAI          18  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          572  CCCGGG               CCC^GGG
SphI          593  GCATGC               GCATG^C
XhoII         641  AGATCT               [GA]^GATC[CT]
XmaCI         572  CCCGGG               C^CCGGG
XmaI          572  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Aor51HI        85  AGCGCT               AGC^GCT
              977  AGCGCT              
AspHI         370  GTGCTC               G[AT]GC[AT]^C
              946  GTGCTC              
BsgI          898  CTGCAC               GTGCAG(16/14)
             1077  CTGCAC              
BsiHKAI       370  GTGCTC               G[AT]GC[AT]^C
              946  GTGCTC              
BsrI          623  ACTGG                ACTGG(1/-1)
              600  CCAGT               
BstF5I        675  CATCC                GGATG(2/0)
              828  CATCC               
DpnII         501  GATC                 ^GATC
              642  GATC                
DraII         464  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              518  GGGTCCT             
DsaI          182  CCGTGG               C^C[GA][CT]GG
             1052  CCACGG              
EagI          170  CGGCCG               C^GGCCG
             1011  CGGCCG              
EarI           43  CTCTTC               CTCTTC(1/4)
              655  CTCTTC              
EclXI         170  CGGCCG               C^GGCCG
             1011  CGGCCG              
Eco47III       85  AGCGCT               AGC^GCT
              977  AGCGCT              
Eco52I        170  CGGCCG               C^GGCCG
             1011  CGGCCG              
EcoO109I      464  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              518  GGGTCCT             
EcoT14I       488  CCAAGG               C^C[AT][AT]GG
              746  CCAAGG              
FokI          675  CATCC                GGATG(9/13)
              828  CATCC               
Ksp632I        43  CTCTTC               CTCTTC(1/4)
              655  CTCTTC              
MaeII         200  ACGT                 A^CGT
              337  ACGT                
MboI          501  GATC                 ^GATC
              642  GATC                
MspA1I        615  CAGCTG               C[AC]G^C[GT]G
              880  CCGCTG              
NdeII         501  GATC                 ^GATC
              642  GATC                
Sau3AI        501  GATC                 ^GATC
              642  GATC                
StyI          488  CCAAGG               C^C[AT][AT]GG
              746  CCAAGG              
TspRI         584  CAGTG                CA[GC]TG[ACGT][ACGT]^
              723  CAGTG               
XcmI          320  CCAAGTACCTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              545  CCATCATGGTCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaII       BanI        BbeI        BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsiEI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrFI       BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        EaeI        EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        KasI        MaeIII      MboII       MnlI        
MslI        MspI        MvaI        MvnI        MwoI        
NarI        NciI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       SfaNI       SfoI        TaqI        
TseI        Tsp45I      TthHB8I     
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