Restriction map for pF1KB7197
 (Circular) (Six-base) MAPSORT of: pF1KB7197.seq  Check: 3685  from: 1  to: 4229



pF1KB7197  4229 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:   1267    1267 
   Size:       4229
 
Acc65I G'GTAC_C

Cuts at:   1245    1245 
   Size:       4229
 
AclI AA'CG_TT

Cuts at:   4069    4069 
   Size:       4229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1125    2054    2486    3233    3501    1125 
   Size:        929     432     747     268    1853

  Fragments arranged by size: 

                1853     929     747     432     268
 
AfeI AGC'GCT

Cuts at:    195    1087     195 
   Size:        892    3337
 
AflIII A'CryG_T

Cuts at:   1581    2685    3662    1581 
   Size:       1104     977    2148

  Fragments arranged by size: 

                2148    1104     977
 
AgeI A'CCGG_T

Cuts at:   2617    2617 
   Size:       4229
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    493     714     493 
   Size:        221    4008
 
AlwNI CAG_nnn'CTG

Cuts at:   1534    3101    1534 
   Size:       1567    2662
 
ApaLI G'TGCA_C

Cuts at:   2999    2999 
   Size:       4229
 
ApoI r'AATT_y

Cuts at:   1233    3582    3838    1233 
   Size:       2349     256    1624

  Fragments arranged by size: 

                2349    1624     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4229
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4229
 
AvaI C'yCGr_G

Cuts at:    680    1249     680 
   Size:        569    3660
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    603     636     603 
   Size:         33    4196
 
BamHI G'GATC_C

Cuts at:   1254    1254 
   Size:       4229
 
BanI G'GyrC_C

Cuts at:    130     199     777     910     930    1245    1659    1907    1942 
   Size:         69     578     133      20     315     414     248      35

Cuts at:   1942     130 
   Size:       2417

  Fragments arranged by size: 

                2417     578     414     315     248     133      69      35
                  20
 
BanII G_rGCy'C

Cuts at:    853    1243    2273     853 
   Size:        390    1030    2809

  Fragments arranged by size: 

                2809    1030     390
 
BbeI G_GCGC'C

Cuts at:    134     203     914     934    1663    1911     134 
   Size:         69     711      20     729     248    2452

  Fragments arranged by size: 

                2452     729     711     248      69      20
 
BbsI GAAGACnn'nnnn_

Cuts at:    355     355 
   Size:       4229
 
BciVI GTATCCnnnnn_n'

Cuts at:   2127    2888    4229    2127 
   Size:        761    1341    2127

  Fragments arranged by size: 

                2127    1341     761
 
BclI T'GATC_A

Cuts at:    608    1749     608 
   Size:       1141    3088
 
BglII A'GATC_T

Cuts at:    749    1744    3781     749 
   Size:        995    2037    1197

  Fragments arranged by size: 

                2037    1197     995
 
BlpI GC'TnA_GC

Cuts at:   1341    1341 
   Size:       4229
 
Bme1580I G_kGCm'C

Cuts at:    653     782    1854    1947    3003     653 
   Size:        129    1072      93    1056    1879

  Fragments arranged by size: 

                1879    1072    1056     129      93
 
BmgBI CAC'GTC

Cuts at:    446     446 
   Size:       4229
 
BmrI ACTGGGnnnn_n'

Cuts at:    741    1441    1522    1857    4001     741 
   Size:        700      81     335    2144     969

  Fragments arranged by size: 

                2144     969     700     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2596    2596 
   Size:       4229
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    691     691 
   Size:       4229
 
Bpu10I CC'TnA_GC

Cuts at:    384     384 
   Size:       4229
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1405    2284    2776    3074    1405 
   Size:        879     492     298    2560

  Fragments arranged by size: 

                2560     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3933    3933 
   Size:       4229
 
BsaAI yAC'GTr

Cuts at:   1582    1582 
   Size:       4229
 
BsaBI GATnn'nnATC

Cuts at:      6     613    1743    3780       6 
   Size:        607    1130    2037     455

  Fragments arranged by size: 

                2037    1130     607     455
 
BsaHI Gr'CG_yC

Cuts at:    131     170     200     911     931    1660    1908    2610    4106 
   Size:         39      30     711      20     729     248     702    1496

Cuts at:   4106     131 
   Size:        254

  Fragments arranged by size: 

                1496     729     711     702     254     248      39      30
                  20
 
BsaWI w'CCGG_w

Cuts at:    332    1418    1939    2617    2891    3038    3449     332 
   Size:       1086     521     678     274     147     411    1112

  Fragments arranged by size: 

                1112    1086     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    190     300     715     721    1023    1132    1654    2412    2989 
   Size:        110     415       6     302     109     522     758     577

Cuts at:   2989    3485     190 
   Size:        496     934

  Fragments arranged by size: 

                 934     758     577     522     496     415     302     110
                 109       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    991    1170     991 
   Size:        179    4050
 
BsiEI CG_ry'CG

Cuts at:    104     281     978    1122    1771    1817    3025     104 
   Size:        177     697     144     649      46    1208    1308

  Fragments arranged by size: 

                1308    1208     697     649     177     144      46
 
BsiHKAI G_wGCw'C

Cuts at:    482    1058    1243    2021    2211    3003     482 
   Size:        576     185     778     190     792    1708

  Fragments arranged by size: 

                1708     792     778     576     190     185
 
BsmBI CGTCTCn'nnnn_

Cuts at:    436     436 
   Size:       4229
 
Bsp1286I G_dGCh'C

Cuts at:    482     653     782     853    1058    1243    1854    1947    2021 
   Size:        171     129      71     205     185     611      93      74

Cuts at:   2021    2211    2273    3003     482 
   Size:        190      62     730    1708

  Fragments arranged by size: 

                1708     730     611     205     190     185     171     129
                  93      74      71      62
 
BspEI T'CCGG_A

Cuts at:   3449    3449 
   Size:       4229
 
BspHI T'CATG_A

Cuts at:   3405    3405 
   Size:       4229
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1279    1493    1795    2176    1279 
   Size:        214     302     381    3332

  Fragments arranged by size: 

                3332     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     150    2521    2575    4059    4224      50 
   Size:        100    2371      54    1484     165      55

  Fragments arranged by size: 

                2371    1484     165     100      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2141    2141 
   Size:       4229
 
BsrFI r'CCGG_y

Cuts at:    114     127     977    2227    2408    2617     114 
   Size:         13     850    1250     181     209    1726

  Fragments arranged by size: 

                1726    1250     850     209     181      13
 
BssHII G'CGCG_C

Cuts at:    224     224 
   Size:       4229
 
BssSI C'ACGA_G

Cuts at:   2500    2858    2500 
   Size:        358    3871
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1115    1606    1115 
   Size:        491    3738
 
BstBI TT'CG_AA

Cuts at:   2590    2590 
   Size:       4229
 
BstEII G'GTnAC_C

Cuts at:    741     741 
   Size:       4229
 
BstYI r'GATC_y

Cuts at:      1     749    1254    1721    1744    2079    2325    3337    3423 
   Size:        748     505     467      23     335     246    1012      86

Cuts at:   3423    3435    3781       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     748     505     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    636     636 
   Size:       4229
 
BtgI C'CryG_G

Cuts at:    107     290    1160     107 
   Size:        183     870    3176

  Fragments arranged by size: 

                3176     870     183
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    534    1002    2179    2360    2523     534 
   Size:        468    1177     181     163    2240

  Fragments arranged by size: 

                2240    1177     468     181     163
 
BtsI GCAGTG_nn'

Cuts at:    698     837    1472     698 
   Size:        139     635    3455

  Fragments arranged by size: 

                3455     635     139
 
DraI TTT'AAA

Cuts at:   1228    1228 
   Size:       4229
 
DraIII CAC_nnn'GTG

Cuts at:    800     800 
   Size:       4229
 
DrdI GACnn_nn'nnGTC

Cuts at:   1935    2793    1935 
   Size:        858    3371
 
EaeI y'GGCC_r

Cuts at:    278     353     398    1119    1814    1988    2379    2406    3751 
   Size:         75      45     721     695     174     391      27    1345

Cuts at:   3751     278 
   Size:        756

  Fragments arranged by size: 

                1345     756     721     695     391     174      75      45
                  27
 
EagI C'GGCC_G

Cuts at:    278    1119    1814     278 
   Size:        841     695    2693

  Fragments arranged by size: 

                2693     841     695
 
EarI CTCTTCn'nnn_

Cuts at:    157     769    2252    2462     157 
   Size:        612    1483     210    1924

  Fragments arranged by size: 

                1924    1483     612     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    114    1084    2437    2747    2893    4039     114 
   Size:        970    1353     310     146    1146     304

  Fragments arranged by size: 

                1353    1146     970     310     304     146
 
EcoICRI GAG'CTC

Cuts at:   1241    1241 
   Size:       4229
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    691    1125    2054    2486    3233    3501     691 
   Size:        434     929     432     747     268    1419

  Fragments arranged by size: 

                1419     929     747     434     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    573     627    1368     573 
   Size:         54     741    3434

  Fragments arranged by size: 

                3434     741      54
 
EcoRI G'AATT_C

Cuts at:   1233    1233 
   Size:       4229
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1107    2248    1107 
   Size:       1141    3088
 
FspI TGC'GCA

Cuts at:    829    2010     829 
   Size:       1181    3048
 
HaeII r_GCGC'y

Cuts at:    134     185     197     203     236     914     934    1089    1217 
   Size:         51      12       6      33     678      20     155     128

Cuts at:   1217    1663    1911    2933    3912     134 
   Size:        446     248    1022     979     451

  Fragments arranged by size: 

                1022     979     678     451     446     248     155     128
                  51      33      20      12       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2071    2101    2103    2133    2071 
   Size:         30       2      30    4167

  Fragments arranged by size: 

                4167      30      30       2
 
HincII GTy'rAC

Cuts at:    540    1268     540 
   Size:        728    3501
 
KasI G'GCGC_C

Cuts at:    130     199     910     930    1659    1907     130 
   Size:         69     711      20     729     248    2452

  Fragments arranged by size: 

                2452     729     711     248      69      20
 
KpnI G_GTAC'C

Cuts at:   1249    1249 
   Size:       4229
 
MluI A'CGCG_T

Cuts at:   3662    3662 
   Size:       4229
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2900    3084    2900 
   Size:        184    4045
 
MscI TGG'CCA

Cuts at:    400    1990     400 
   Size:       1590    2639
 
MslI CAynn'nnrTG

Cuts at:    343     529     661     667     796     889    2218    2345     343 
   Size:        186     132       6     129      93    1329     127    2227

  Fragments arranged by size: 

                2227    1329     186     132     129     127      93       6
            
 
MspA1I CmG'CkG

Cuts at:    725     990    1340    1654    2014    3027    3272     725 
   Size:        265     350     314     360    1013     245    1682

  Fragments arranged by size: 

                1682    1013     360     350     314     265     245
 
NaeI GCC'GGC

Cuts at:    129    2410     129 
   Size:       2281    1948
 
NarI GG'CG_CC

Cuts at:    131     200     911     931    1660    1908     131 
   Size:         69     711      20     729     248    2452

  Fragments arranged by size: 

                2452     729     711     248      69      20
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4229
 
NgoMIV G'CCGG_C

Cuts at:    127    2408     127 
   Size:       2281    1948
 
NotI GC'GGCC_GC

Cuts at:   1119    1119 
   Size:       4229
 
NspI r_CATG'y

Cuts at:    705    1282    1782    2689     705 
   Size:        577     500     907    2245

  Fragments arranged by size: 

                2245     907     577     500
 
PciI A'CATG_T

Cuts at:   2685    2685 
   Size:       4229
 
PmeI GTTT'AAAC

Cuts at:   1228    1228 
   Size:       4229
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3368    3400    3368 
   Size:         32    4197
 
PpuMI rG'GwC_Cy

Cuts at:    627     627 
   Size:       4229
 
PstI C_TGCA'G

Cuts at:    143    1276    1961     143 
   Size:       1133     685    2411

  Fragments arranged by size: 

                2411    1133     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4229
 
PvuII CAG'CTG

Cuts at:    725    1654    2014     725 
   Size:        929     360    2940

  Fragments arranged by size: 

                2940     929     360
 
RsrII CG'GwC_CG

Cuts at:   2424    2424 
   Size:       4229
 
SacI G_AGCT'C

Cuts at:   1243    1243 
   Size:       4229
 
SalI G'TCGA_C

Cuts at:   1266    1266 
   Size:       4229
 
SapI GCTCTTCn'nnn_

Cuts at:    157    2252    2462     157 
   Size:       2095     210    1924

  Fragments arranged by size: 

                2095    1924     210
 
SbfI CC_TGCA'GG

Cuts at:   1276    1276 
   Size:       4229
 
ScaI AGT'ACT

Cuts at:   3746    3746 
   Size:       4229
 
SfcI C'TryA_G

Cuts at:     33     139    1272    1957    2950    3141      33 
   Size:        106    1133     685     993     191    1121

  Fragments arranged by size: 

                1133    1121     993     685     191     106
 
SfoI GGC'GCC

Cuts at:    132     201     912     932    1661    1909     132 
   Size:         69     711      20     729     248    2452

  Fragments arranged by size: 

                2452     729     711     248      69      20
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4229
 
SgrAI Cr'CCGG_yG

Cuts at:    127     127 
   Size:       4229
 
SmaI CCC'GGG

Cuts at:    682    1251     682 
   Size:        569    3660
 
SmlI C'TyrA_G

Cuts at:   1384    2299    2791    3053    1384 
   Size:        915     492     262    2560

  Fragments arranged by size: 

                2560     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4229
 
SphI G_CATG'C

Cuts at:    705    1282    1782     705 
   Size:        577     500    3152

  Fragments arranged by size: 

                3152     577     500
 
StyI C'CwwG_G

Cuts at:    107     596     854    1363    1717     107 
   Size:        489     258     509     354    2619

  Fragments arranged by size: 

                2619     509     489     354     258
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1812    1945    2404    2613    1812 
   Size:        133     459     209    3428

  Fragments arranged by size: 

                3428     459     209     133
 
TatI w'GTAC_w

Cuts at:   3744    3744 
   Size:       4229
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3746    3778    3746 
   Size:         32    4197
 
Tth111I GACn'n_nGTC

Cuts at:   2026    2026 
   Size:       4229
 
XbaI T'CTAG_A

Cuts at:   1260    1260 
   Size:       4229
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    435     660    1724     435 
   Size:        225    1064    2940

  Fragments arranged by size: 

                2940    1064     225
 
XmaI C'CCGG_G

Cuts at:    680    1249     680 
   Size:        569    3660
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NspI     PciI
     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII    BbvCI     BcgI    BfrBI     BglI     BmtI    BsaXI    BseRI
    BsiWI     BsmI    BsrGI    BstXI  BstZ17I     ClaI    CspCI    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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