Restriction map for pF1KB7195
 (Circular) (Six-base) MAPSORT of: pF1KB7195.seq  Check: 8827  from: 1  to: 3500



pF1KB7195  3500 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:    332     382     538     332 
   Size:         50     156    3294

  Fragments arranged by size: 

                3294     156      50
 
Acc65I G'GTAC_C

Cuts at:    516     516 
   Size:       3500
 
AclI AA'CG_TT

Cuts at:   3340    3340 
   Size:       3500
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1325    1757    2504    2772    1325 
   Size:        432     747     268    2053

  Fragments arranged by size: 

                2053     747     432     268
 
AflIII A'CryG_T

Cuts at:    310     852    1956    2933     310 
   Size:        542    1104     977     877

  Fragments arranged by size: 

                1104     977     877     542
 
AgeI A'CCGG_T

Cuts at:   1888    1888 
   Size:       3500
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    174     174 
   Size:       3500
 
AlwNI CAG_nnn'CTG

Cuts at:    470     805    2372     470 
   Size:        335    1567    1598

  Fragments arranged by size: 

                1598    1567     335
 
ApaI G_GGCC'C

Cuts at:    464     464 
   Size:       3500
 
ApaLI G'TGCA_C

Cuts at:   2270    2270 
   Size:       3500
 
ApoI r'AATT_y

Cuts at:    504    2853    3109     504 
   Size:       2349     256     895

  Fragments arranged by size: 

                2349     895     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3500
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3500
 
AvaI C'yCGr_G

Cuts at:    520     520 
   Size:       3500
 
BamHI G'GATC_C

Cuts at:    525     525 
   Size:       3500
 
BanI G'GyrC_C

Cuts at:    516     930    1178    1213     516 
   Size:        414     248      35    2803

  Fragments arranged by size: 

                2803     414     248      35
 
BanII G_rGCy'C

Cuts at:    464     514    1544     464 
   Size:         50    1030    2420

  Fragments arranged by size: 

                2420    1030      50
 
BbeI G_GCGC'C

Cuts at:    934    1182     934 
   Size:        248    3252
 
BbsI GAAGACnn'nnnn_

Cuts at:    118     118 
   Size:       3500
 
BciVI GTATCCnnnnn_n'

Cuts at:   1398    2159    3500    1398 
   Size:        761    1341    1398

  Fragments arranged by size: 

                1398    1341     761
 
BclI T'GATC_A

Cuts at:   1020    1020 
   Size:       3500
 
BglII A'GATC_T

Cuts at:   1015    3052    1015 
   Size:       2037    1463
 
BlpI GC'TnA_GC

Cuts at:    612     612 
   Size:       3500
 
Bme1580I G_kGCm'C

Cuts at:    353     464    1125    1218    2274     353 
   Size:        111     661      93    1056    1579

  Fragments arranged by size: 

                1579    1056     661     111      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    385     396     712     793    1128    3272     385 
   Size:         11     316      81     335    2144     613

  Fragments arranged by size: 

                2144     613     335     316      81      11
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1867    1867 
   Size:       3500
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    103     103 
   Size:       3500
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    676    1555    2047    2345     676 
   Size:        879     492     298    1831

  Fragments arranged by size: 

                1831     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3204    3204 
   Size:       3500
 
BsaAI yAC'GTr

Cuts at:    853     853 
   Size:       3500
 
BsaBI GATnn'nnATC

Cuts at:      6    1014    3051       6 
   Size:       1008    2037     455

  Fragments arranged by size: 

                2037    1008     455
 
BsaHI Gr'CG_yC

Cuts at:    931    1179    1881    3377     931 
   Size:        248     702    1496    1054

  Fragments arranged by size: 

                1496    1054     702     248
 
BsaWI w'CCGG_w

Cuts at:    689    1210    1888    2162    2309    2720     689 
   Size:        521     678     274     147     411    1469

  Fragments arranged by size: 

                1469     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    285     463     925    1683    2260    2756     285 
   Size:        178     462     758     577     496    1029

  Fragments arranged by size: 

                1029     758     577     496     462     178
 
BsiEI CG_ry'CG

Cuts at:    104    1042    1088    2296     104 
   Size:        938      46    1208    1308

  Fragments arranged by size: 

                1308    1208     938      46
 
BsiHKAI G_wGCw'C

Cuts at:    363     514    1292    1482    2274     363 
   Size:        151     778     190     792    1589

  Fragments arranged by size: 

                1589     792     778     190     151
 
Bsp1286I G_dGCh'C

Cuts at:    353     363     464     514    1125    1218    1292    1482    1544 
   Size:         10     101      50     611      93      74     190      62

Cuts at:   1544    2274     353 
   Size:        730    1579

  Fragments arranged by size: 

                1579     730     611     190     101      93      74      62
                  50      10
 
BspEI T'CCGG_A

Cuts at:   2720    2720 
   Size:       3500
 
BspHI T'CATG_A

Cuts at:   2676    2676 
   Size:       3500
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    550     764    1066    1447     550 
   Size:        214     302     381    2603

  Fragments arranged by size: 

                2603     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1792    1846    3330    3495      50 
   Size:       1742      54    1484     165      55

  Fragments arranged by size: 

                1742    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    188    1412     188 
   Size:       1224    2276
 
BsrFI r'CCGG_y

Cuts at:   1498    1679    1888    1498 
   Size:        181     209    3110

  Fragments arranged by size: 

                3110     209     181
 
BssSI C'ACGA_G

Cuts at:   1771    2129    1771 
   Size:        358    3142
 
BstAPI GCAn_nnn'nTGC

Cuts at:    877     877 
   Size:       3500
 
BstBI TT'CG_AA

Cuts at:   1861    1861 
   Size:       3500
 
BstXI CCAn_nnnn'nTGG

Cuts at:    270     270 
   Size:       3500
 
BstYI r'GATC_y

Cuts at:      1     267     525     992    1015    1350    1596    2608    2694 
   Size:        266     258     467      23     335     246    1012      86

Cuts at:   2694    2706    3052       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     266     258     246
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:    333     383     333 
   Size:         50    3450
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1450    1631    1794    1450 
   Size:        181     163    3156

  Fragments arranged by size: 

                3156     181     163
 
BtsI GCAGTG_nn'

Cuts at:    743     743 
   Size:       3500
 
DraI TTT'AAA

Cuts at:    499     499 
   Size:       3500
 
DraIII CAC_nnn'GTG

Cuts at:    242     404     242 
   Size:        162    3338
 
DrdI GACnn_nn'nnGTC

Cuts at:   1206    2064    1206 
   Size:        858    2642
 
EaeI y'GGCC_r

Cuts at:    395    1085    1259    1650    1677    3022     395 
   Size:        690     174     391      27    1345     873

  Fragments arranged by size: 

                1345     873     690     391     174      27
 
EagI C'GGCC_G

Cuts at:   1085    1085 
   Size:       3500
 
EarI CTCTTCn'nnn_

Cuts at:   1523    1733    1523 
   Size:        210    3290
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1708    2018    2164    3310    1708 
   Size:        310     146    1146    1898

  Fragments arranged by size: 

                1898    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    512     512 
   Size:       3500
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    103    1325    1757    2504    2772     103 
   Size:       1222     432     747     268     831

  Fragments arranged by size: 

                1222     831     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    282     460     639     282 
   Size:        178     179    3143

  Fragments arranged by size: 

                3143     179     178
 
EcoRI G'AATT_C

Cuts at:    504     504 
   Size:       3500
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1519    1519 
   Size:       3500
 
FspI TGC'GCA

Cuts at:   1281    1281 
   Size:       3500
 
HaeII r_GCGC'y

Cuts at:    934    1182    2204    3183     934 
   Size:        248    1022     979    1251

  Fragments arranged by size: 

                1251    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1342    1372    1374    1404    1342 
   Size:         30       2      30    3438

  Fragments arranged by size: 

                3438      30      30       2
 
HincII GTy'rAC

Cuts at:    539     539 
   Size:       3500
 
KasI G'GCGC_C

Cuts at:    930    1178     930 
   Size:        248    3252
 
KpnI G_GTAC'C

Cuts at:    520     520 
   Size:       3500
 
MluI A'CGCG_T

Cuts at:   2933    2933 
   Size:       3500
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2171    2355    2171 
   Size:        184    3316
 
MscI TGG'CCA

Cuts at:    397    1261     397 
   Size:        864    2636
 
MslI CAynn'nnrTG

Cuts at:   1489    1616    1489 
   Size:        127    3373
 
MspA1I CmG'CkG

Cuts at:    611     925    1285    2298    2543     611 
   Size:        314     360    1013     245    1568

  Fragments arranged by size: 

                1568    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1681    1681 
   Size:       3500
 
NarI GG'CG_CC

Cuts at:    931    1179     931 
   Size:        248    3252
 
NgoMIV G'CCGG_C

Cuts at:   1679    1679 
   Size:       3500
 
NspI r_CATG'y

Cuts at:    314     553    1053    1960     314 
   Size:        239     500     907    1854

  Fragments arranged by size: 

                1854     907     500     239
 
PciI A'CATG_T

Cuts at:    310    1956     310 
   Size:       1646    1854
 
PmeI GTTT'AAAC

Cuts at:    499     499 
   Size:       3500
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2639    2671    2639 
   Size:         32    3468
 
PpuMI rG'GwC_Cy

Cuts at:    282     282 
   Size:       3500
 
PspOMI G'GGCC_C

Cuts at:    460     460 
   Size:       3500
 
PstI C_TGCA'G

Cuts at:    547    1232     547 
   Size:        685    2815
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3500
 
PvuII CAG'CTG

Cuts at:    925    1285     925 
   Size:        360    3140
 
RsrII CG'GwC_CG

Cuts at:   1695    1695 
   Size:       3500
 
SacI G_AGCT'C

Cuts at:    514     514 
   Size:       3500
 
SalI G'TCGA_C

Cuts at:    537     537 
   Size:       3500
 
SapI GCTCTTCn'nnn_

Cuts at:   1523    1733    1523 
   Size:        210    3290
 
SbfI CC_TGCA'GG

Cuts at:    547     547 
   Size:       3500
 
ScaI AGT'ACT

Cuts at:   3017    3017 
   Size:       3500
 
SfcI C'TryA_G

Cuts at:     33     543    1228    2221    2412      33 
   Size:        510     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     510     191
 
SfoI GGC'GCC

Cuts at:    932    1180     932 
   Size:        248    3252
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3500
 
SmaI CCC'GGG

Cuts at:    522     522 
   Size:       3500
 
SmlI C'TyrA_G

Cuts at:    655    1570    2062    2324     655 
   Size:        915     492     262    1831

  Fragments arranged by size: 

                1831     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3500
 
SphI G_CATG'C

Cuts at:    553    1053     553 
   Size:        500    3000
 
StuI AGG'CCT

Cuts at:    455     455 
   Size:       3500
 
StyI C'CwwG_G

Cuts at:    634     988     634 
   Size:        354    3146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    415    1083    1216    1675    1884     415 
   Size:        668     133     459     209    2031

  Fragments arranged by size: 

                2031     668     459     209     133
 
TatI w'GTAC_w

Cuts at:    442    3015     442 
   Size:       2573     927
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3017    3049    3017 
   Size:         32    3468
 
Tth111I GACn'n_nGTC

Cuts at:   1297    1297 
   Size:       3500
 
XbaI T'CTAG_A

Cuts at:    531     531 
   Size:       3500
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    409     995     409 
   Size:        586    2914
 
XmaI C'CCGG_G

Cuts at:    520     520 
   Size:       3500
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PmeI
     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII   Bsu36I     BtgI     ClaI    CspCI    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI
    PshAI     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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