Restriction map for pF1KB7187
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BciVI       BclI        BfrI        BlnI        BsaAI       
BsaBI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrBI       
BsrGI       BssHII      Bst1107I    BstXI       BstZ17I     
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MscI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsaI        RsrII       SacII       SalI        SbfI        
ScaI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII       1388  TCCGGA               T^CCGGA
AcsI         1384  AAATTC               [GA]^AATT[CT]
AlwI         1462  GATCC                GGATC(4/5)
ApoI         1384  AAATTC               [GA]^AATT[CT]
BbeI          228  GGCGCC               GGCGC^C
BbsI         1598  GTCTTC               GAAGAC(2/6)
BcgI         1001  GCAGCAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         669  AGATCT               A^GATCT
BlpI          480  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      480  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1598  GTCTTC               GAAGAC(2/6)
BsaWI        1388  TCCGGA               [AT]^CCGG[AT]
BseAI        1388  TCCGGA               T^CCGGA
BspEI        1388  TCCGGA               T^CCGGA
BspHI        1310  TCATGA               T^CATGA
BsrDI        1434  CATTGC               GCAATG(2/0)
BssSI         391  CACGAG               CACGAG(-5/-1)
BstAPI       1067  GCACTGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         637  TTCGAA               TT^CGAA
BstYI         669  AGATCT               [GA]^GATC[CT]
CelII         480  GCTGAGC              GC^T[ACGT]AGC
DraIII       1068  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1274  CCGTGG               C^C[GA][CT]GG
EcoRV         616  GATATC               GAT^ATC
HaeII         228  GGCGCC               [GA]GCGC^[CT]
HgaI          217  GCGTC                GACGC(5/10)
KasI          228  GGCGCC               G^GCGCC
MflI          669  AGATCT               [GA]^GATC[CT]
MroI         1388  TCCGGA               T^CCGGA
MslI         1450  CACCCAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          228  GGCGCC               GG^CGCC
NspI          960  GCATGC               [GA]CATG^[CT]
NspV          637  TTCGAA               TT^CGAA
PpuMI         395  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1310  TCATGA               T^CATGA
SacI         1402  GAGCTC               GAGCT^C
SexAI        1163  ACCTGGT              A^CC[AT]GGT
SfiI           78  GGCCTCTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          228  GGCGCC               GGC^GCC
SfuI          637  TTCGAA               TT^CGAA
SphI          960  GCATGC               GCATG^C
StuI          318  AGGCCT               AGG^CCT
TfiI          784  GAATC                G^A[AT]TC
XhoII         669  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          216  GGCGTC               G[GA]^CG[CT]C
              228  GGCGCC              
AlwNI         197  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              488  CAGCTTCTG           
AocI          430  CCTGAGG              CC^T[ACGT]AGG
             1014  CCTCAGG             
ApaI           21  GGGCCC               GGGCC^C
              928  GGGCCC              
AspHI        1402  GAGCTC               G[AT]GC[AT]^C
             1469  GTGCTC              
BsaHI         216  GGCGTC               G[GA]^CG[CT]C
              228  GGCGCC              
BsaI          730  GAGACC               GGTCTC(1/5)
              875  GAGACC              
BsiHKAI      1402  GAGCTC               G[AT]GC[AT]^C
             1469  GTGCTC              
BsmAI         730  GAGAC                GTCTC(1/5)
              875  GAGAC               
BspMI        1343  ACCTGC               ACCTGC(4/8)
             1190  GCAGGT              
Bsu36I        430  CCTGAGG              CC^T[ACGT]AGG
             1014  CCTCAGG             
DpnII         670  GATC                 ^GATC
             1462  GATC                
Eco81I        430  CCTGAGG              CC^T[ACGT]AGG
             1014  CCTCAGG             
EcoNI        1184  CCTTCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1506  CCTGAAAGAGG         
EcoT14I       503  CCAAGG               C^C[AT][AT]GG
              515  CCTTGG              
Hin1I         216  GGCGTC               G[GA]^CG[CT]C
              228  GGCGCC              
MboI          670  GATC                 ^GATC
             1462  GATC                
MseI         1526  TTAA                 T^TAA
             1612  TTAA                
NaeI           93  GCCGGC               GCC^GGC
              187  GCCGGC              
NdeII         670  GATC                 ^GATC
             1462  GATC                
NgoMIV         93  GCCGGC               G^CCGGC
              187  GCCGGC              
PflMI         196  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              950  CCAGTCGGTGG         
PstI          265  CTGCAG               CTGCA^G
             1188  CTGCAG              
SapI          127  GAAGAGC              GCTCTTC(1/4)
             1305  GCTCTTC             
Sau3AI        670  GATC                 ^GATC
             1462  GATC                
SmaI           24  CCCGGG               CCC^GGG
              931  CCCGGG              
StyI          503  CCAAGG               C^C[AT][AT]GG
              515  CCTTGG              
Tru9I        1526  TTAA                 T^TAA
             1612  TTAA                
Tsp509I      1385  AATT                 ^AATT
             1609  AATT                
Van91I        196  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              950  CCAGTCGGTGG         
XmaCI          24  CCCGGG               C^CCGGG
              931  CCCGGG              
XmaI           24  CCCGGG               C^CCGGG
              931  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DraII       EaeI        
EarI        EcoO109I    EcoO65I     EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PleI        Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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