Restriction map for pF1KB7187
 (Linear) (Six-base) MAPSORT of: KIBB7187.seq  Check: 3823  from: 1  to: 1617



KIBB7187.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1181    1617 
   Size:       1181     436
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1585    1617 
   Size:       1585      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     202     493    1617 
   Size:        202     291    1124

  Fragments arranged by size: 

                1124     291     202
 
ApaI G_GGCC'C

Cuts at:      0      25     932    1617 
   Size:         25     907     685

  Fragments arranged by size: 

                 907     685      25
 
ApoI r'AATT_y

Cuts at:      0    1384    1617 
   Size:       1384     233
 
AvaI C'yCGr_G

Cuts at:      0      24     400     931    1617 
   Size:         24     376     531     686

  Fragments arranged by size: 

                 686     531     376      24
 
BanI G'GyrC_C

Cuts at:      0     228     289     589    1617 
   Size:        228      61     300    1028

  Fragments arranged by size: 

                1028     300     228      61
 
BanII G_rGCy'C

Cuts at:      0      25     487     514     602     932    1282    1406    1447 
   Size:         25     462      27      88     330     350     124      41

Cuts at:   1447    1617 
   Size:        170

  Fragments arranged by size: 

                 462     350     330     170     124      88      41      27
                  25
 
BbeI G_GCGC'C

Cuts at:      0     232    1617 
   Size:        232    1385
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1591    1617 
   Size:       1591      26
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     990    1024    1617 
   Size:        990      34     593

  Fragments arranged by size: 

                 990     593      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85      94    1082    1617 
   Size:         85       9     988     535

  Fragments arranged by size: 

                 988     535      85       9
 
BglII A'GATC_T

Cuts at:      0     669    1617 
   Size:        669     948
 
BlpI GC'TnA_GC

Cuts at:      0     481    1617 
   Size:        481    1136
 
Bme1580I G_kGCm'C

Cuts at:      0      25     592     908     932    1617 
   Size:         25     567     316      24     685

  Fragments arranged by size: 

                 685     567     316      25      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     920    1617 
   Size:        920     697
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     616     762    1038    1617 
   Size:        364     252     146     276     579

  Fragments arranged by size: 

                 579     364     276     252     146
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542    1333    1480    1576    1617 
   Size:        542     791     147      96      41

  Fragments arranged by size: 

                 791     542     147      96      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     724     869    1617 
   Size:        724     145     748

  Fragments arranged by size: 

                 748     724     145
 
BsaHI Gr'CG_yC

Cuts at:      0     217     229    1617 
   Size:        217      12    1388

  Fragments arranged by size: 

                1388     217      12
 
BsaWI w'CCGG_w

Cuts at:      0    1388    1617 
   Size:       1388     229
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1036    1066    1617 
   Size:       1036      30     551

  Fragments arranged by size: 

                1036     551      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     341     847     850     859    1528    1531    1617 
   Size:        341     506       3       9     669       3      86

  Fragments arranged by size: 

                 669     506     341      86       9       3       3
 
BseYI C'CCAG_C

Cuts at:      0     202     486    1617 
   Size:        202     284    1131

  Fragments arranged by size: 

                1131     284     202
 
BsiHKAI G_wGCw'C

Cuts at:      0    1406    1473    1617 
   Size:       1406      67     144

  Fragments arranged by size: 

                1406     144      67
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     487     514     592     602     908     932    1282 
   Size:         25     462      27      78      10     306      24     350

Cuts at:   1282    1406    1447    1473    1617 
   Size:        124      41      26     144

  Fragments arranged by size: 

                 462     350     306     144     124      78      41      27
                  26      25      24      10
 
BspEI T'CCGG_A

Cuts at:      0    1388    1617 
   Size:       1388     229
 
BspHI T'CATG_A

Cuts at:      0    1310    1617 
   Size:       1310     307
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1181    1352    1617 
   Size:       1181     171     265

  Fragments arranged by size: 

                1181     265     171
 
BsrDI GCAATG_nn'

Cuts at:      0    1433    1617 
   Size:       1433     184
 
BsrFI r'CCGG_y

Cuts at:      0      93     187     286     606    1109    1617 
   Size:         93      94      99     320     503     508

  Fragments arranged by size: 

                 508     503     320      99      94      93
 
BssSI C'ACGA_G

Cuts at:      0     391    1617 
   Size:        391    1226
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1073    1617 
   Size:       1073     544
 
BstBI TT'CG_AA

Cuts at:      0     638    1617 
   Size:        638     979
 
BstEII G'GTnAC_C

Cuts at:      0     282     689    1570    1617 
   Size:        282     407     881      47

  Fragments arranged by size: 

                 881     407     282      47
 
BstYI r'GATC_y

Cuts at:      0     669    1617 
   Size:        669     948
 
Bsu36I CC'TnA_GG

Cuts at:      0     431    1015    1617 
   Size:        431     584     602

  Fragments arranged by size: 

                 602     584     431
 
BtgI C'CryG_G

Cuts at:      0    1274    1617 
   Size:       1274     343
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     368     993    1617 
   Size:        368     625     624

  Fragments arranged by size: 

                 625     624     368
 
BtsI GCAGTG_nn'

Cuts at:      0      43    1086    1617 
   Size:         43    1043     531

  Fragments arranged by size: 

                1043     531      43
 
DraIII CAC_nnn'GTG

Cuts at:      0    1073    1617 
   Size:       1073     544
 
EaeI y'GGCC_r

Cuts at:      0     245    1152    1271    1617 
   Size:        245     907     119     346

  Fragments arranged by size: 

                 907     346     245     119
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     251     824     836    1132    1312    1617 
   Size:        122     129     573      12     296     180     305

  Fragments arranged by size: 

                 573     305     296     180     129     122      12
 
EcoICRI GAG'CTC

Cuts at:      0    1404    1617 
   Size:       1404     213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     616     762    1038    1585    1617 
   Size:        364     252     146     276     547      32

  Fragments arranged by size: 

                 547     364     276     252     146      32
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1188    1510    1617 
   Size:       1188     322     107

  Fragments arranged by size: 

                1188     322     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     363     396     928    1019    1181    1617 
   Size:         21     342      33     532      91     162     436

  Fragments arranged by size: 

                 532     436     342     162      91      33      21
 
EcoRV GAT'ATC

Cuts at:      0     618    1617 
   Size:        618     999
 
HaeII r_GCGC'y

Cuts at:      0     232    1617 
   Size:        232    1385
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234    1036    1068    1405    1437    1504    1536 
   Size:        202      32     802      32     337      32      67      32

Cuts at:   1536    1617 
   Size:         81

  Fragments arranged by size: 

                 802     337     202      81      67      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0     228    1617 
   Size:        228    1389
 
MslI CAynn'nnrTG

Cuts at:      0    1454    1617 
   Size:       1454     163
 
MspA1I CmG'CkG

Cuts at:      0     199     350     449    1617 
   Size:        199     151      99    1168

  Fragments arranged by size: 

                1168     199     151      99
 
NaeI GCC'GGC

Cuts at:      0      95     189    1617 
   Size:         95      94    1428

  Fragments arranged by size: 

                1428      95      94
 
NarI GG'CG_CC

Cuts at:      0     229    1617 
   Size:        229    1388
 
NgoMIV G'CCGG_C

Cuts at:      0      93     187    1617 
   Size:         93      94    1430

  Fragments arranged by size: 

                1430      94      93
 
NspI r_CATG'y

Cuts at:      0     964    1617 
   Size:        964     653
 
PasI CC'CwG_GG

Cuts at:      0     685    1617 
   Size:        685     932
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     202     956    1617 
   Size:        202     754     661

  Fragments arranged by size: 

                 754     661     202
 
PfoI T'CCnGG_A

Cuts at:      0     739    1484    1617 
   Size:        739     745     133

  Fragments arranged by size: 

                 745     739     133
 
PpuMI rG'GwC_Cy

Cuts at:      0     396    1617 
   Size:        396    1221
 
PspOMI G'GGCC_C

Cuts at:      0      21     928    1617 
   Size:         21     907     689

  Fragments arranged by size: 

                 907     689      21
 
PstI C_TGCA'G

Cuts at:      0     269    1192    1617 
   Size:        269     923     425

  Fragments arranged by size: 

                 923     425     269
 
SacI G_AGCT'C

Cuts at:      0    1406    1617 
   Size:       1406     211
 
SapI GCTCTTCn'nnn_

Cuts at:      0     122    1312    1617 
   Size:        122    1190     305

  Fragments arranged by size: 

                1190     305     122
 
SexAI A'CCwGG_T

Cuts at:      0    1163    1617 
   Size:       1163     454
 
SfcI C'TryA_G

Cuts at:      0     265     437    1188    1617 
   Size:        265     172     751     429

  Fragments arranged by size: 

                 751     429     265     172
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      85    1617 
   Size:         85    1532
 
SfoI GGC'GCC

Cuts at:      0     230    1617 
   Size:        230    1387
 
SmaI CCC'GGG

Cuts at:      0      26     933    1617 
   Size:         26     907     684

  Fragments arranged by size: 

                 907     684      26
 
SmlI C'TyrA_G

Cuts at:      0     557    1348    1495    1555    1617 
   Size:        557     791     147      60      62

  Fragments arranged by size: 

                 791     557     147      62      60
 
SphI G_CATG'C

Cuts at:      0     964    1617 
   Size:        964     653
 
SrfI GCCC'GGGC

Cuts at:      0      26    1617 
   Size:         26    1591
 
StuI AGG'CCT

Cuts at:      0     320    1617 
   Size:        320    1297
 
StyI C'CwwG_G

Cuts at:      0     503     515    1617 
   Size:        503      12    1102

  Fragments arranged by size: 

                1102     503      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     981    1466    1617 
   Size:        981     485     151

  Fragments arranged by size: 

                 981     485     151
 
XmaI C'CCGG_G

Cuts at:      0      24     931    1617 
   Size:         24     907     686

  Fragments arranged by size: 

                 907     686      24
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BbeI     BbsI     BcgI     BglI    BglII     BlpI Bme1580I     BmrI
     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
    HaeII    Hin4I     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI     PstI     SacI
     SapI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SphI
     SrfI     StuI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI    BmgBI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BsrBI    BsrGI   BssHII    BstXI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoRI     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI
     ScaI     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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