Restriction map for pF1KB7186
 (Linear) (Six-base) MAPSORT of: KIBB7186.seq  Check: 363  from: 1  to: 1311



KIBB7186.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1169    1311 
   Size:       1169     142
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     849    1121    1311 
   Size:        849     272     190

  Fragments arranged by size: 

                 849     272     190
 
AflIII A'CryG_T

Cuts at:      0     914    1311 
   Size:        914     397
 
AleI CACnn'nnGTG

Cuts at:      0     508    1311 
   Size:        508     803
 
AlwNI CAG_nnn'CTG

Cuts at:      0     426     645     744     872    1311 
   Size:        426     219      99     128     439

  Fragments arranged by size: 

                 439     426     219     128      99
 
ApaI G_GGCC'C

Cuts at:      0     163    1307    1311 
   Size:        163    1144       4

  Fragments arranged by size: 

                1144     163       4
 
ApaLI G'TGCA_C

Cuts at:      0    1295    1311 
   Size:       1295      16
 
ApoI r'AATT_y

Cuts at:      0    1117    1311 
   Size:       1117     194
 
AvaI C'yCGr_G

Cuts at:      0      18     124     139     155     162    1311 
   Size:         18     106      15      16       7    1149

  Fragments arranged by size: 

                1149     106      18      16      15       7
 
BanII G_rGCy'C

Cuts at:      0      25     110     125     140     156     163     179     787 
   Size:         25      85      15      15      16       7      16     608

Cuts at:    787     911    1307    1311 
   Size:        124     396       4

  Fragments arranged by size: 

                 608     396     124      85      25      16      16      15
                  15       7       4
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     820    1311 
   Size:        820     491
 
BlpI GC'TnA_GC

Cuts at:      0     427     520     991    1311 
   Size:        427      93     471     320

  Fragments arranged by size: 

                 471     427     320      93
 
Bme1580I G_kGCm'C

Cuts at:      0     163     200    1260    1299    1307    1311 
   Size:        163      37    1060      39       8       4

  Fragments arranged by size: 

                1060     163      39      37       8       4
 
BmgBI CAC'GTC

Cuts at:      0     456    1311 
   Size:        456     855
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     320    1227    1311 
   Size:        320     907      84

  Fragments arranged by size: 

                 907     320      84
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      90    1311 
   Size:         90    1221
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     640     925    1066    1311 
   Size:        302     338     285     141     245

  Fragments arranged by size: 

                 338     302     285     245     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204    1311 
   Size:        204    1107
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     320     570    1311 
   Size:        320     250     741

  Fragments arranged by size: 

                 741     320     250
 
BsaAI yAC'GTr

Cuts at:      0     915    1238    1311 
   Size:        915     323      73

  Fragments arranged by size: 

                 915     323      73
 
BsaWI w'CCGG_w

Cuts at:      0     110    1036    1311 
   Size:        110     926     275

  Fragments arranged by size: 

                 926     275     110
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     300     330    1311 
   Size:        300      30     981

  Fragments arranged by size: 

                 981     300      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     346    1055    1311 
   Size:        346     709     256

  Fragments arranged by size: 

                 709     346     256
 
BseYI C'CCAG_C

Cuts at:      0     546     932    1021    1176    1311 
   Size:        546     386      89     155     135

  Fragments arranged by size: 

                 546     386     155     135      89
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1311 
   Size:       1006     305
 
BsiEI CG_ry'CG

Cuts at:      0     889    1311 
   Size:        889     422
 
BsiHKAI G_wGCw'C

Cuts at:      0     787    1034    1299    1311 
   Size:        787     247     265      12

  Fragments arranged by size: 

                 787     265     247      12
 
BsmI GAATG_Cn'

Cuts at:      0     538    1311 
   Size:        538     773
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     446    1311 
   Size:        446     865
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     110     125     140     156     163     179     200 
   Size:         25      85      15      15      16       7      16      21

Cuts at:    200     787     911    1034    1260    1299    1307    1311 
   Size:        587     124     123     226      39       8       4

  Fragments arranged by size: 

                 587     226     124     123      85      39      25      21
                  16      16      15      15       8       7       4
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     815    1169    1311 
   Size:        815     354     142

  Fragments arranged by size: 

                 815     354     142
 
BsrBI CCG'CTC

Cuts at:      0     339    1311 
   Size:        339     972
 
BsrGI T'GTAC_A

Cuts at:      0     470    1311 
   Size:        470     841
 
BssSI C'ACGA_G

Cuts at:      0     616     730    1311 
   Size:        616     114     581

  Fragments arranged by size: 

                 616     581     114
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     375     645    1311 
   Size:        375     270     666

  Fragments arranged by size: 

                 666     375     270
 
BstYI r'GATC_y

Cuts at:      0     360    1199    1311 
   Size:        360     839     112

  Fragments arranged by size: 

                 839     360     112
 
Bsu36I CC'TnA_GG

Cuts at:      0     587    1311 
   Size:        587     724
 
BtgI C'CryG_G

Cuts at:      0     385     893    1260    1311 
   Size:        385     508     367      51

  Fragments arranged by size: 

                 508     385     367      51
 
BtsI GCAGTG_nn'

Cuts at:      0     842    1299    1311 
   Size:        842     457      12

  Fragments arranged by size: 

                 842     457      12
 
DraIII CAC_nnn'GTG

Cuts at:      0    1266    1311 
   Size:       1266      45
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     460    1311 
   Size:        460     851
 
EaeI y'GGCC_r

Cuts at:      0     722     886    1311 
   Size:        722     164     425

  Fragments arranged by size: 

                 722     425     164
 
EagI C'GGCC_G

Cuts at:      0     886    1311 
   Size:        886     425
 
EarI CTCTTCn'nnn_

Cuts at:      0     144     421    1311 
   Size:        144     277     890

  Fragments arranged by size: 

                 890     277     144
 
EcoICRI GAG'CTC

Cuts at:      0     785    1311 
   Size:        785     526
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     640     849     925    1066    1121    1311 
   Size:        302     338     209      76     141      55     190

  Fragments arranged by size: 

                 338     302     209     190     141      76      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159     296    1303    1304    1311 
   Size:        159     137    1007       1       7

  Fragments arranged by size: 

                1007     159     137       7       1
 
HaeII r_GCGC'y

Cuts at:      0     244    1055    1311 
   Size:        244     811     256

  Fragments arranged by size: 

                 811     256     244
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     300     332     345     377     585     617     785     817 
   Size:        300      32      13      32     208      32     168      32

Cuts at:    817    1311 
   Size:        494

  Fragments arranged by size: 

                 494     300     208     168      32      32      32      32
                  13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     993    1311 
   Size:        993     318
 
MscI TGG'CCA

Cuts at:      0     724    1311 
   Size:        724     587
 
MslI CAynn'nnrTG

Cuts at:      0     508    1311 
   Size:        508     803
 
MspA1I CmG'CkG

Cuts at:      0     118     387     645     990    1180    1311 
   Size:        118     269     258     345     190     131

  Fragments arranged by size: 

                 345     269     258     190     131     118
 
NspI r_CATG'y

Cuts at:      0     471    1311 
   Size:        471     840
 
PasI CC'CwG_GG

Cuts at:      0     633     922    1311 
   Size:        633     289     389

  Fragments arranged by size: 

                 633     389     289
 
PfoI T'CCnGG_A

Cuts at:      0     902    1311 
   Size:        902     409
 
PmlI CAC'GTG

Cuts at:      0     915    1238    1311 
   Size:        915     323      73

  Fragments arranged by size: 

                 915     323      73
 
PspOMI G'GGCC_C

Cuts at:      0     159    1303    1311 
   Size:        159    1144       8

  Fragments arranged by size: 

                1144     159       8
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     832     864    1311 
   Size:        832      32     447

  Fragments arranged by size: 

                 832     447      32
 
PstI C_TGCA'G

Cuts at:      0     650     812    1010    1166    1311 
   Size:        650     162     198     156     145

  Fragments arranged by size: 

                 650     198     162     156     145
 
PvuII CAG'CTG

Cuts at:      0     645     990    1180    1311 
   Size:        645     345     190     131

  Fragments arranged by size: 

                 645     345     190     131
 
SacI G_AGCT'C

Cuts at:      0     787    1311 
   Size:        787     524
 
SacII CC_GC'GG

Cuts at:      0     388    1311 
   Size:        388     923
 
SapI GCTCTTCn'nnn_

Cuts at:      0     144     421    1311 
   Size:        144     277     890

  Fragments arranged by size: 

                 890     277     144
 
SexAI A'CCwGG_T

Cuts at:      0     560    1311 
   Size:        560     751
 
SfcI C'TryA_G

Cuts at:      0     646     808    1006    1162    1311 
   Size:        646     162     198     156     149

  Fragments arranged by size: 

                 646     198     162     156     149
 
SmaI CCC'GGG

Cuts at:      0      20     126     157    1311 
   Size:         20     106      31    1154

  Fragments arranged by size: 

                1154     106      31      20
 
SmlI C'TyrA_G

Cuts at:      0     183    1311 
   Size:        183    1128
 
SrfI GCCC'GGGC

Cuts at:      0      20     126    1311 
   Size:         20     106    1185

  Fragments arranged by size: 

                1185     106      20
 
StyI C'CwwG_G

Cuts at:      0     951    1109    1190    1311 
   Size:        951     158      81     121

  Fragments arranged by size: 

                 951     158     121      81
 
TatI w'GTAC_w

Cuts at:      0     470    1311 
   Size:        470     841
 
Tth111I GACn'n_nGTC

Cuts at:      0     312    1311 
   Size:        312     999
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     900     919    1311 
   Size:        900      19     392

  Fragments arranged by size: 

                 900     392      19
 
XmaI C'CCGG_G

Cuts at:      0      18     124     155    1311 
   Size:         18     106      31    1156

  Fragments arranged by size: 

                1156     106      31      18
 
XmnI GAAnn'nnTTC

Cuts at:      0     862    1311 
   Size:        862     449
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    BanII     BbsI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrGI    BssSI   BstAPI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NspI     PasI     PfoI
     PmlI   PspOMI     PsrI     PstI    PvuII     SacI    SacII     SapI
    SexAI     SfcI     SmaI     SmlI     SrfI     StyI     TatI  Tth111I
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BmtI   Bpu10I    BsaBI    BsaHI    BsiWI    BspEI    BspHI
    BsrDI    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI     EciI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PvuI    RsrII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI
     ZraI
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